In patients with chronic myeloid leukemia who do not reach a (near) complete cytogenetic response upon treatment, the disease progresses over months to several years from an indolent, chronic phase ...into a rapidly fatal blast crisis. Events that are responsible for this transformation process are largely unknown. To identify changes occurring in the course of the disease, we performed cDNA subtraction on sequentially stored peripheral blood mononuclear cell pellets, collected from a single CML patient throughout the course of disease. Thirty-three differentially expressed sequences were identified, of which 28 corresponded to known genes. Quantitative PCR confirmed cDNA subtraction results for 24 of 25 tested genes. Differential expression levels ranged from <2- to 106-fold. The sequences that were identified include genes encoding for several apoptosis related proteins, cell cycle proteins, interleukins/chemokines, heat shock proteins and a DNA repair enzyme (see table). Thus far, expression levels of eight genes have been tested in sequential samples of three to six additional CML patients, gathered throughout their disease course. PBCEF, IL8, IL6, CCL4 and YWHAZ show comparable patterns as the index patient; expression levels of APEX, NXF1 and PRG1 were consistent with those of the index patient in only a part of the additional patients. More patients are being tested on these and remaining genes. We conclude that this set of genes can be considered as a valuable starting point for further research on the causes of disease transformation in CML.
Name of geneAccession No.Fold difference (Q-PCR)diagnosis > blast crisis:PRG1NM_00272714IVNS1ABPNM_006469<2MPONM_000250NDblast crisis > diagnosis:EMR1NM_001974<2CD96NM_1981963GAS2NM_00525612CHI3L2NM_00400011late chronic phase > blast crisis:IL8NM_0005842S100A8NM_00296416FPR1NM_00202951LYZNM_000239NDMEN1AF001893**blast crisis > late chronic phase:MMRNNM_0073516NACSINNM_01525224LARSNM_0201177FLJ25286*NM_152546NDC5orf13***NM_004772NDC15orf15***NM_016304NDFLJ10849*NM_018243NDdiagnosis > late chronic phase:DNAJB1NM_00614535HSPA8NM_0065975NXF1NM_0063622DC2NM_021227<2APEX1NM_0806484RPS6NM_001010<2EEF1A1NM_0014023FLJ25286*NM_152546NDlate chronic phase > diagnosis:SATNM_0029707CCL4NM_002984106YWHAZNM_1456903PBEF1NM_1827907IL6NM_00060042IL8NM_0005849* Genes hypothetically encoding proteins, ** Reverse to subtraction result, ***orf: open reading frame
The LHCb detector has undergone a major upgrade for LHC Run 3. This Upgrade I detector facilitates operation at higher luminosity and utilises full-detector information at the LHC collision rate, ...critically including the use of vertex information. A new vertex locator system, the VELO Upgrade, has been constructed. The core element of the new VELO are the double-sided pixelated hybrid silicon detector modules which operate in vacuum close to the LHC beam in a high radiation environment. The construction and quality assurance tests of these modules are described in this paper. The modules incorporate 200 \mum thick, n-on-p silicon sensors bump-bonded to 130 \nm technology ASICs. These are attached with high precision to a silicon microchannel substrate that uses evaporative CO\(_2\) cooling. The ASICs are controlled and read out with flexible printed circuits that are glued to the substrate and wire-bonded to the chips. The mechanical support of the module is given by a carbon fibre plate, two carbon fibre rods and an aluminium plate. The sensor attachment was achieved with an average precision of 21 \(\mathrm{\mu m}\), more than 99.5\% of all pixels are fully functional, and a thermal figure of merit of 3 \mathrm{Kcm^{2}W^{-1}}$ was achieved. The production of the modules was successfully completed in 2021, with the final assembly and installation completed in time for data taking in 2022.
Illustrations p. vii -- Introduction p. 1 -- Part I. Beyond the Maternal Ideal p. 25 -- Part II. "Bad Mothers" : Caretaking, Class, and Maternal Violence p. 101 -- Part III. Maternity and Difference ...: Nation, Race, and Empire p. 181 -- Part IV. The Maternal Body p. 271 -- Contributors 335 -- Index 339
Eight nonreproductive female Drakensberg crag lizards (Pseudocordylus melanotus melanotus) were each fed diets of mealworms and calcium capsules with various calcium levels. Excreta were collected ...and analyzed for calcium and uric acid content. The amount of calcium in the feces was calculated. The lizards appeared to be able to maintain calcium balance at calcium intakes equivalent to 1.4-5.6% calcium in the dry matter of feed. Calcium balance was maintained by adapting intestinal calcium absorption.
APPARATUS Klaver, N S; Kuizenga, K; Ballast, A ...
Anaesthesia,
07/2007, Letnik:
62, Številka:
7
Journal Article
Recenzirano
The Laryngeal Tube S(TM) and the LMA-ProSeal(R) are supraglottic instruments with an improved airway seal and a drainage tube to protect against regurgitation and to facilitate passage of a gastric ...tube. We compared the feasibility of these two instruments in a randomised, controlled clinical trial. One hundred and sixty patients were randomly allocated to undergo insertion of a Laryngeal Tube S (n = 82) or an LMA-ProSeal (n = 78). All insertions were carried out by first-month anaesthesia residents. Success rates were not significantly different: Laryngeal Tube S 89%, LMA-ProSeal 95%. There was also no significant difference in leak pressure or insertion time. Insertion time decreased significantly when we compared the first with the last 10 insertions. Gastric tube placement was successful in all patients in the Laryngeal Tube S group, but failed in 12 patients in the LMA-ProSeal group (p < 0.001). Dysphagia was reported by 22% of Laryngeal Tube S group and 3% of LMA-ProSeal group (p = 0.001). These findings demonstrate the applicability of the devices and a learning effect in the hands of anaesthesia residents with limited experience. PUBLICATION ABSTRACT
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