In recent years, much concern has been addressed over the soil contamination with heavy metals due to rapid industrialization and urbanization. The present study was conducted to investigate ...distribution of potentially hazardous elements (PHEs) (As, Cr, Cu, Ni, Pb and Zn) concentration in soils of Kazipalli, Hyderabad, India. Soil samples from fifty-seven (57) sampling sites were collected from in and around industrial zone and were analysed for their heavy metal contents. Concentrations ranged from 4.4 to 796.3 mg/kg for As, 9.7 to 598.6 mg/kg for Cr, 7.9 to 183.5 mg/kg for Cu, 10.2 to 129.6 mg/kg for Ni, 25.3 to 1830 mg/kg for Pb and 23.8 to 879 mg/kg for Zn. Application of Pearson’s correlation, factor and cluster analysis indicates that heavy metal contamination in soils originates from industrial activities which are of anthropogenic origin. Contamination of soils in the study area was further classified for geoaccumulation index, enrichment factor, contamination factor and contamination degree. The values of pollution index and integrated pollution index indicated that metal pollution levels were in order of As > Pb > Cu > Cr > Zn > Ni. Potential ecological risk indices (PERI, RI) and health risk assessment based on Hazard Quotient, Hazard index and on Average daily doses of individual elements were calculated using exposure parameters for resident population and references from integrated database of USEPA. These results are important for the development of proper management strategies to decrease point and non-point source of pollution by studying different remediation methods.
Undulating landscapes of Chhotanagpur plateau of the Indian state of Jharkhand suffer from soil erosion vulnerability of varying degrees. An investigation was undertaken in some sections of the Upper ...Subarnarekha River Basin falling within this state. An empirical equation known as Universal Soil Loss Equation (USLE) was utilized for estimating the soil loss. Analysis of remote sensing satellite data, digital elevation model (DEM) and geographical information system (GIS)–based geospatial approach together with USLE led to the soil erosion assessment. Erosion vulnerability assessment was performed by analyzing raster grids of topography acquired from Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) Global DEM data. LANDSAT TM and ETM+ satellite data of March 2001 and March 2011 were used for inferring the land use–land cover characteristics of the watershed for these years, respectively. USLE equation was computed within the GIS framework to derive annual soil erosion rates and also the areas with varying degrees of erosion vulnerability. Erosion vulnerability units thus identified covered five severity classes of erosion ranging from very low (0–5 ton ha⁻¹ yr⁻¹) to very severe (> 40 ton ha⁻¹ yr⁻¹). Results indicated an overall increase of erosion in the year 2011 as compared to the erosion computed for the year 2001. Maximum soil erosion rate during the year 2001 was found up to 40 ton ha⁻¹ yr⁻¹, whereas this went up to 49.80 ton ha⁻¹ yr⁻¹ for the year 2011. Factors for the increase in overall erosion could be variation in rainfall, decrease in vegetation or protective land covers and most important but not limited to the increase in built-up or impervious areas as well.
Traumatic brain injury (TBI) is one of the foremost causes of disability and death globally. Prerequisites for successful therapy of disabilities associated with TBI involved improved knowledge of ...the neurobiology of TBI, measurement of quantitative changes in recovery dynamics brought about by therapy, and the translation of quantitative methodologies and techniques that were successful in tracking recovery in preclinical models to human TBI. Frequently used animal models of TBI in research and development include controlled cortical impact, fluid percussion injury, blast injury, penetrating blast brain injury, and weight-drop impact acceleration models. Preclinical models of TBI benefit from controlled injury settings and the best prospects for biometric quantification of injury and therapy-induced gradual recovery from disabilities. Impact acceleration closed head TBI paradigm causes diffuse TBI (DTBI) without substantial focal brain lesions in rats. DTBI is linked to a significant rate of death, morbidity, and long-term disability. DTBI is difficult to diagnose at the time of hospitalization with imaging techniques making it challenging to take prompt therapeutic action. The weight-drop method without craniotomy is an impact acceleration closed head DTBI model that is used to induce mild/moderate diffuse brain injuries in rodents. Additionally, we have characterized neuropathological and neurobehavioral outcomes of the weight-drop model without craniotomy for inducing closed head DTBI of graded severity with a range of mass of weights (50-450 gm). This chapter also discusses techniques and protocols for measuring numerous functional disabilities and pathological changes in the brain brought on by DTBI.
Dynamic loading conditions, such as earthquakes, may result in the generation of high shear strain (>5%) in the soil. Conventionally, dynamic properties of soils are estimated from the tests ...conducted up to a shear strain of 1% by considering Symmetrical Hysteresis Loop (SHL). However, it is commonly observed that the hysteresis loops become progressively asymmetric with increasing shear strain, which leads to the over- or under-estimation of the conventionally evaluated dynamic properties. Hence, it is necessary to adopt a modified methodology of evaluating the dynamic properties of saturated sands based on the actual Asymmetrical Hysteresis Loop (ASHL). Strain-controlled cyclic triaxial tests have been conducted, for a peak shear strain range of 0.015–4.5% at 1Hz loading frequency, on test specimens prepared at different relative density (30–90%) and confining stress (50–150kPa). Although, the shear modulus evaluated considering SHL and ASHL are on close agreement, the damping ratio evaluated considering SHL is approximately 40–70% lesser than that obtained by considering ASHL. Moreover, in contrast to the classical curves as largely applied in geotechnical engineering, a noticeable decrement of the damping ratio is observed beyond 0.75% shear strain.
•Evaluation of high strain (0.01–4.5%) dynamic properties using cyclic triaxial test.•Development of asymmetrical hysteresis loop under high strain.•Applying a modified methodology to evaluate the dynamic properties for asymmetrical loop.•Damping ratio from asymmetrical loop, D#, is 40–70% higher than that of symmetrical loop, D.•Beyond peak, noticeable decrement in D and D# at a shear strain of 0.5% and 1% respectively.
This study assessed the snow/ice surface area changes over the Zemu Glacier in the Eastern Himalayas. Zemu is considered to be the largest glacier in the Eastern Himalayas located in the Sikkim State ...of India. Change detection in the snow/ice surface areal extent of the Zemu Glacier was delineated from the year 1945 using US Army Map Service-Topographical Sheets and Landsat imageries available from 1987 to 2020. The results obtained solely focus on surface-change delineation using remote sensing satellite data and GIS software. Landsat imagery of the years 1987, 1997, 2009, 2018, and 2020 was used for snow and ice pixel extraction. Normalized Difference Snow Index (NDSI), Snow Cover Index (S3), and new band ratio index were used to extract the pure snow and ice pixels, fresh snow, and debris-covered snow/ice area and pixels mixed with shadow to demarcate the surface area changes. Manual delineation was required and undertaken for better results. A slope raster image was generated from Shuttle Radar Topography Mission (SRTM) digital elevation model (DEM) data to delineate the slope and hill shade. The results illustrate that the snow/ice surface covering the glacier had an area of 113.5 km
2
in the year 1945 which was reduced to 78.31 km
2
in the year 2020 indicating a total snow/ice surface area loss of 31% in 75 years. A loss of 11.45% was observed from 1945 to 1987 in the areal extent, while from 1987 to 2009, a decadal loss of approximately 7% was observed. The surface area loss of 8.46% from 2009 to 2018 led to infer the maximum loss of snow and ice over the glacier body at a rate of 0.94% per year. Between the years 2018 and 2020, the glacier lost 1.08% of its surface area. The Accumulation Area Ratio (AAR) considering the accumulation and ablation areas of the glacier indicated a gradual decline of the accumulation zone in the recent years. Global Land Ice Measurement from Space (GLIMS) program data with RGI version 6.0 was used as a reference to demarcate the areal extent of Zemu Glacier. The study gained above 80% of overall accuracy by generating a confusion matrix in ArcMap. The analysis of this seasonal snow/ice cover showed that there has been a drastic decline in the surface snow/ice cover area over the Zemu Glacier over the years 1987 to 2020 and NDSI; S3 analysis provided improved results to delineate snow/ice cover area over such steep terrain topography of Sikkim Himalaya.
Aggregation-induced emission enhancement (AIEE) of thiolated gold nanoclusters (AuNCs) has emerged as an attractive and alternative strategy to improve their brightness. This study demonstrates ...Ce(iii)-triggered AIEE of glutathione-capped AuNCs (GSH-AuNCs) through the coordination between two carboxylic groups of GSH and Ce(iii). The cluster size and valence state of GSH-AuNCs are almost identical to those of a Ce(iii)-induced assembly of GSH-AuNCs (named Ce(iii)-GSH-AuNCs). More importantly, the as-prepared Ce(iii)-GSH-AuNCs exhibit a higher quantum yield (up to 13%), longer luminescence lifetime, and shorter maximum luminescence peak than GSH-AuNCs. Additionally, Ce(iii)-GSH-AuNCs possess redox-switchable luminescence, high salt stability, and long-term storage stability. These findings provide clear evidence that the Ce(iii)-triggered aggregation of GSH-AuNCs is a crucial factor to improve the luminescence property of GSH-AuNCs. Intriguingly, the presence of adenosine triphosphate (ATP) switches off the luminescence of Ce(iii)-GSH AuNCs through the significant formation of Ce(iii)-ATP complexes. Furthermore, the ATP-induced luminescence quenching of Ce(iii)-GSH-AuNCs can be paired with the alkaline phosphatase (ALP)-ATP system to design a turn-on luminescent probe for ALP; the limit of detection for ALP is estimated to be 0.03 U L-1. Also, the biocompatibility of Ce(iii)-GSH-AuNCs enables the proposed system to detect ALP in human serum and HeLa cells.
Collagen I, the most abundant extracellular matrix (ECM) protein in vertebrate tissues provides mechanical durability to tissue microenvironments and regulates cell function. Its fibrillogenesis in ...biological milieu is predominantly regulated by dermatan sulfate proteoglycans, proteins conjugated with iduronic acid‐containing dermatan sulfate (DS) glycosaminoglycans (GAG). Although DS is known to regulate tissue function through its modulation of Coll I architecture, a precise understanding of the latter remains elusive. We investigated this problem by visualizing the fibrillar pattern of fixed Coll I gels polymerized in the presence of varying concentrations of DS using second harmonic generation microscopy. Measuring mean second harmonic generation signal (which estimates the ordering of the fibrils), and surface occupancy (which estimates the space occupied by fibrils) supported by confocal reflectance microscopy, our observations indicated that the effect on fibril pattern of DS is contextual upon the latter's concentrations: Lower levels of DS resulted in sparse disorganized fibrils; higher levels restore organization, with fibrils occupying greater space. An appropriate change in elasticity as a result of DS levels was also observed through atomic force microscopy. Examination of dye‐based GAG staining and scanning electron microscopy suggested distinct constitutions of Coll I gels when polymerized with higher and lower levels of DS. We observed that adhesion of the invasive ovarian cancer cells SKOV3 decreased for lower DS levels but was partially restored at higher DS levels. Our study shows how the Coll I gel pattern‐tuning of DS is of relevance for understanding its biomaterial applications and possibly, pathophysiological functions.
ABSTRACT
Blazar variability can be described as flaring activities on a wide range of time-scales over a baseline flux level. It is important to detect and distinguish baseline flux changes from ...long-term flare variations, since the former may reflect state transitions caused by a secular change in bulk properties such as the bulk Lorentz factor or the viewing angle. We report such a transition observed in the 11 yr Fermi γ-ray light curve of the blazar 3C 66A, where the baseline flux of ∼1.8 × 10−7 ph cm−2 s−1 persisted for three years and then changed over a month time-scale to ∼0.8 × 10−7 ph cm−2 s−1 and remained in that level for the next eight years. Moreover, there is evidence for a similar shift in baseline flux in the optical band. Broad-band spectral energy distribution modelling for the two activity states reveals that the baseline flux change is consistent with an overall decrease in the Doppler factor, which in turn implies an increase in the jet inclination angle by ∼1° or a decrease in the bulk Lorentz factor by ∼25 per cent. We discuss the implication of such a variation occurring on a month time-scale.
Glioblastoma multiforme (GBM) is the most aggressive form of brain tumor characterized by inter and intra-tumor heterogeneity and complex tumor microenvironment. To uncover the molecular targets in ...this milieu, we systematically identified immune and stromal interactions at the glial cell type level that leverages on RNA-sequencing data of GBM patients from The Cancer Genome Atlas. The perturbed genes between the high vs low immune and stromal scored patients were subjected to weighted gene co-expression network analysis to identify the glial cell type specific networks in immune and stromal infiltrated patients. The intramodular connectivity analysis identified the highly connected genes in each module. Combining it with univariable and multivariable prognostic analysis revealed common vital gene ITGB2, between the immune and stromal infiltrated patients enriched in microglia and newly formed oligodendrocytes. We found following unique hub genes in immune infiltrated patients; COL6A3 (microglia), ITGAM (oligodendrocyte precursor cells), TNFSF9 (microglia), and in stromal infiltrated patients, SERPINE1 (microglia) and THBS1 (newly formed oligodendrocytes, oligodendrocyte precursor cells). To validate these hub genes, we used external GBM patient single cell RNA-sequencing dataset and this identified ITGB2 to be significantly enriched in microglia, newly formed oligodendrocytes, T-cells, macrophages and adipocyte cell types in both immune and stromal datasets. The tumor infiltration analysis of ITGB2 showed that it is correlated with myeloid dendritic cells, macrophages, monocytes, neutrophils, B-cells, fibroblasts and adipocytes. Overall, the systematic screening of tumor microenvironment components at glial cell types uncovered ITGB2 as a potential target in primary GBM.
Display omitted
•In-silico analysis characterising GBM immune-TME at glial cell type level.•WGCNA and survival analysis highlights vital regulators.•Single cell analysis reveals ITGB2 as immune and stromal-related gene in microglia and NFO.•ITGB2 positive correlation with tumour infiltration of immune and stromal cells.
Diabetes is a metabolic disorder and over the past decades, it has become a major cause of morbidity and mortality affecting the youth and middle-aged as it is the fourth leading cause of disease ...related to death. In both type 1 and type 2 diabetes the severe pathogenesis cause micro vascular complications: nephropathy, retinopathy, neuropathy and macro vascular complications: cardiovascular disease, heart attacks and stroke. Under hyperglycemia, activation of different signaling mechanisms such as an increased polyol pathway, advanced-glycation end product formation, activation of Protein Kinase C and hexosamine pathway leads to the over expression of reactive oxygen species and causes pathogenesis of diabetic complications. It is necessary to understand these pathways in diabetic complications causing damage to the secondary system of the body. In the past decade the understanding of these biochemical changes has increased tremendously and various molecules have been exploited as therapeutic targets for diabetic complications as better therapeutic approach. In this review, a brief overview about diabetes mellitus and chronic complications with their current understandings of cellular/molecular mechanisms and targeted therapies along with novel therapeutic strategies is discussed.
PDF
