Phyllanthus species plants are a rich source of phenolics and widely used due to their medicinal properties. A liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed using ...high-pressure liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HPLC-ESI-QTOFMS/MS) for the identification and characterization of quercetin, kaempferol, ellagic acid and their derivatives in ethanolic extracts of Phyllanthus species. The chromatographic separation was carried out on Thermo Betasil C8 column (250 mm×4.5 mm, 5 μm) using 0.1% formic acid in water and 0.1% formic acid in methanol as the mobile phase. The identification of diagnostic fragment ions and optimization of collision energies were carried out using 21 reference standards. Totally 51 compounds were identified which include 21 compounds identifiedand characterized unambiguously by comparison with their authentic standards and the remaining 30 were tentatively identified and characterized in ethanolic extracts of P. emblica, P. fraternus, P. amarus and P.niruri.
Environmental biotechnology is the use of biotechnology to develop and regulate biological systems for the remediation of environmental contamination. Nature has gifted ample material for remediation ...of its resources, among which chitosan is one of the most important and largely available biomaterial globally. Chitosan is a biopolymer obtained by deacetylation of chitin extracted from marine waste and its applications from drug delivery to food additives are broadly available. Chitosan exhibit several properties such as availability, low cost, high biocompatibility, and biodegradability. These properties make it biologically and chemically acceptable for use in various fields. Due to some limitations of pure chitosan, there has been a growing interest in modifying the chitosan in order to improve the original properties and widen the applications of pure phase chitosan. Various modified forms of chitosan and their associated applications are reviewed here with emphasis on their use in environmental remediation. The demand of chitosan in the global industrial market is growing which is briefly explained in this paper. Chitosan is used for water purification since a long time and still progress is going on for making it more efficient in the removal process. It can be used as a flocculent and coagulant, as an adsorbent for removing the contaminants like heavy metals, dyes, pesticides, antibiotics, biological contaminants from wastewater. Soil remediation using chitosan material is explained in this review. Various other applications such as drug delivery, food additives, tissue engineering are thoroughly reviewed.
•Chitosan exhibit numerous properties which are used in ample of applications.•Biotechnology develops natural systems for environmental remediation.•Hydroxyl and amine groups on chitosan responsible for different modifications.•Application of chitosan for environmental remediation has been compiled here.
Non-thermal plasma (NTP) is one of the most promising minimal processing methods for the food industry. However up until recently there are limited studies which would report the application and ...effect of NTP on processed seafood. The objective of this review is to highlight the recent findings and advancements in the application of NTP within the fish and other seafood industry, including direct application of fresh and dried fish and seafood with as well as indirect application of plasma activated water and seafood industry wastewater purification. The article also summarizes the effect of plasma treatment on microbiological quality, physicochemical and sensory properties and oxidation rate of treated fish and seafood. NTP has high potential to be used within various fields of seafood industry. It is especially effective in treatment of dried seafood products, but the use of plasma activated water during various processing steps such as seafood washing could be also beneficial. Moreover NTP could also be used as a cost effective and environmentally friendly method for seafood wastewater purification.
The present paper is an attempt to put forward a simple thermodynamic model for the computation and prediction of the cohesive energy, melting temperature and Debye temperature for nanosolids ...considering the size, shape, relaxation and packing factors, all at one place for a proper understanding of the thermal stability of nanosolids. We have selected Ag, Al, W, Co and Mo nanoparticles for the study of cohesive energy; Au, Ag, Al and Sn for melting temperature and Co, Au and Fe nanoparticles for Debye temperature in different shapes. We have found that these thermophysical properties decrease as particles are downsized and the shape loses the sphericity. The available experimental data having a close consistency with the computed results proves the validity of the present theoretical exercise. The present theoretical model for the thermophysical properties is a potentially strong candidate for the computation of the properties in the absence of experimental data.
Organic thin film transistor (OTFT) based device modeling and circuit application is a rapidly emerging research area. Taking cognizance of this fact, our paper reviews various basic to advanced OTFT ...structures, their performance parameters, materials of individual OTFT layers, their molecular structures, OTFT charge transport phenomena, and fabrication techniques. The performance of p- and n-type conducting polymer and small molecule organic semiconductors are reviewed primarily in terms of field effect mobility, current on/off ratio, and operating voltage for various OTFT structures. Moreover, different organic/inorganic materials for realizing the dielectric layer, electrodes, and the substrate in an OTFT are analyzed. Some of the compact models that are essential for predicting and optimizing the device performance are described that takes into account the mobility enhancement factor and channel length modulation. A detailed study of the single gate, dual gate, vertical channel, and cylindrical gate OTFT structures is carried out. Furthermore, the paper discusses some of the interesting and upcoming applications of organic transistors such as inverters, light emitting diodes (LEDs), RFID tags, and DNA sensors. Although organic transistors boast of a bright future with a wide spectrum of applications, but they still face several challenges in terms of mobility, voltage swings, noise margins, sub-threshold slope, stability, etc., that needs to be resolved to make them a commercially sustainable and viable technology.
The estrogen-related receptor alpha (ESRRA) is an orphan nuclear receptor (NR) that significantly influences cellular metabolism. ESRRA is predominantly expressed in metabolically-active tissues and ...regulates the transcription of metabolic genes, including those involved in mitochondrial turnover and autophagy. Although ESRRA activity is well-characterized in several types of cancer, recent reports suggest that it also has an important role in metabolic diseases. This minireview focuses on the regulation of cellular metabolism and function by ESRRA and its potential as a target for the treatment of metabolic disorders.
Endophytic actinobacteria play an important role in growth promotion and development of host plant by producing enormous quantities of novel bioactive natural products. In the present investigation, ...169 endophytic actinobacteria were isolated from endospheric tissues of Rhynchotoechum ellipticum. Based on their antimicrobial potential, 81 strains were identified by 16rRNA gene analysis, which were taxonomically grouped into 15 genera. All identified strains were screened for their plant growth promoting attributes and, for the presence of modular polyketide synthases (PKSI, PKSII and nonribosomal peptide synthetase (NRPS) gene clusters to correlate the biosynthetic genes with their functional properties. Expression studies and antioxidant potential for four representative strains were evaluated using qRT-PCR and DPPH assay respectively. Additionally, six antibiotics (erythromycin, ketoconazole, fluconazole, chloramphenicol, rifampicin and miconazole) and nine phenolic compounds (catechin, kaempferol, chebulagic acid, chlorogenic acid, Asiatic acid, ferulic acid, arjunic acid, gallic acid and boswellic acid) were detected and quantified using UHPLC-QqQ
-MS/MS. Furthermore, three strains (BPSAC77, 121 and 101) showed the presence of the anticancerous compound paclitaxel which was reported for the first time from endophytic actinobacteria. This study provides a holistic picture, that endophytic actinobacteria are rich bacterial resource for bioactive natural products, which has a great prospective in agriculture and pharmaceutical industries.
Disruption of Akt and Erk-mediated signal transduction significantly contributes in the pathogenesis of various neurodegenerative diseases (NDs), such as Parkinson’s disease, Alzheimer’s diseases, ...Huntington’s disease, and many others. These regulatory proteins serve as the regulator of cell survival, motility, transcription, metabolism, and progression of the cell cycle. Therefore, targeting Akt and Erk pathway has been proposed as a reasonable approach to suppress ND progression. This review has emphasized on involvement of Akt/Erk cascade in the neurodegeneration. Akt has been reported to regulate neuronal toxicity through its various substrates like FOXos, GSK3β, and caspase-9 etc. Akt is also involved with PI3K in signaling pathway to mediate neuronal survival. ERK is another kinase which also regulates proliferation, differentiation, and survival of the neural cell. There has also been much progress in developing a therapeutic molecule targeting Akt and Erk signaling. Therefore, improved understanding of the molecular mechanism behind the regulatory aspect of Akt and Erk networks can make strong impact on exploration of the neurodegenerative disease pathogenesis.
The present study, deals with the estimation of degrees of contamination, ecological and human health risk of heavy metals (As, Cd, Cr, Cu, Mn, Ni, Pb, and Zn) in sediments, surface water and fishes, ...which were collected from middle stretch of Damodar river and ponds at Asansol, which receives outfall of various coal-based industries. Metal content in the premonsoon season was higher than the postmonsoon, due to influx of rainwater. The heavy metal pollution indices (HPI) at some locations was observed up to 1.45 times than recommended value and the cadmium (Cd) was found dominating metal for high HPI value. The Cd concentration in surface water and pore water varied from 2.8 to 14.9 µg/L and 15.3-57.0 µg/L, respectively, which was up to 6 times higher than the permissible limit. Ecological risk assessment for sediments illustrates 'moderate to considerable ecological risk', especially because of Cd. Hazard index (HI) calculated to identify potential human health risk by dermal exposure of surface water was <0.1, indicating 'negligible non-cancer risk' for all age group of people. However, HI varied from 0.73 to 1.49 for adult and 1.37-2.78 by consumption of fishes indicates children have higher 'non-cancer risk' than adult.
Diabetic foot complications are the most common occurring problems throughout the globe, resulting in devastating economic crises for the patients, families and society. Diabetic foot ulcers (DFUs) ...have a neuropathic origin with a progressive prevalence rate in developing countries compared with developed countries among diabetes mellitus patients. Diabetic patients that are of greatest risk of ulcers may easily be diagnosed with foot examination. Economic burden may be carefully examined. The budget costing must include both the clinical and social impact of the patients.
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