The food safety criteria that have been incorporated in European regulation (EC) No2073/2005 (Official Journal of the European Union L, 338, 2005, 1), for Listeria monocytogenes in ready‐to eat (RTE) ...foods, specify a maximum allowable concentration of 100 CFU g⁻¹or ml⁻¹. Some factors such as pH, salt and modified atmosphere packaging (MAP) are used to prevent the growth of L. monocytogenes in order to comply with the limit. Interactions between background microflora (BM) and L. monocytogenes may limit the growth of L. monocytogenes. The aim of this study was to investigate the mechanisms behind the observed inhibition by natural BM of the growth of L. monocytogenes in ready‐to‐eat diced poultry meat whose pH and water activity were favourable to its growth. The dynamics of L. monocytogenes and natural BM were therefore monitored in mono‐culture and co‐culture experiments with various combinations of contamination levels. In the absence of BM, the growth potential of L. monocytogenes depended only on the initial inoculum. With both BM and L. monocytogenes, whatever the combination of concentrations studied, the growth potentials of L. monocytogenes were lower than in a mono‐culture through a partial Jameson effect. Thus, the use‐by date of this product can be optimized by using models that take into account interactions with BM. SIGNIFICANCE AND IMPACT OF THE STUDY: The study of the growth of Listeria monocytogenes in a diced poultry meat, a matrix whose pH and water activity characteristics are favourable to L. monocytogenes growth, showed that it was inhibited by natural background microflora. This highlights the importance of knowing the product's composition, and in particular the natural background microflora, which can impact the use‐by date.
Filamin A (FlnA) is a ubiquitous actin binding protein which anchors various transmembrane proteins to the cell cytoskeleton and provides a scaffold to many cytoplasmic signaling proteins involved in ...actin cytoskeleton remodeling in response to mechanical stress and cytokines stimulation. Although the vast majority of FlnA binding partners interact with the carboxy-terminal immunoglobulin like (Igl) repeats of FlnA, little is known on the role of the amino-N-terminal repeats. Here, using cardiac mitral valvular dystrophy associated FlnA–G288R and P637Q mutations located in the N-terminal Igl repeat 1 and 4 respectively as a model, we identified a new role of FlnA N-terminal repeats in small Rho-GTPases regulation. Using FlnA-deficient melanoma and HT1080 cell lines as expression systems we showed that FlnA mutations reduce cell spreading and migration capacities. Furthermore, we defined a signaling network in which FlnA mutations alter the balance between RhoA and Rac1 GTPases activities in favor of RhoA and provided evidences for a role of the Rac1 specific GTPase activating protein FilGAP in this process. Together our work ascribed a new role to the N-terminal repeats of FlnA in Small GTPases regulation and supports a conceptual framework for the role of FlnA mutations in cardiac valve diseases centered around signaling molecules regulating cellular actin cytoskeleton in response to mechanical stress.
•FlnA G288R and P637Q restore melanoma cells cortical actin cytoskeleton.•FlnA G288R and P637Q impede cell adhesion, spreading and migration.•FlnA G288R and P637Q deregulate the balance of GTPases.•RhoA–Rac1 deregulation determines spreading deficiencies.•FilGAP is involved in the deregulation and spreading deficiencies.
The production of muons from heavy-flavour hadron decays in p-Pb collisions at $\sqrt{{\textit s}_{\rm NN}}=5.02$ TeV was studied for $2 < p_{\rm T} < 16$ GeV/$c$ with the ALICE detector at the CERN ...LHC. The measurement was performed at forward (p-going direction) and backward (Pb-going direction) rapidity, in the ranges of rapidity in the center-of-mass system (cms) $2.03<y_{\rm cms}<3.53$ and $-4.46<y_{\rm cms}<-2.96$, respectively. The production cross sections and nuclear modification factors are presented as a function of transverse momentum ($p_{\rm T}$). At forward rapidity, the nuclear modification factor is compatible with unity while at backward rapidity, in the interval $2.5<p_{\rm T}<3.5$ GeV/$c$, it is above unity by more than 2$\sigma$. The ratio of the forward-to-backward production cross sections is also measured in the overlapping interval $2.96 < \vert y_{\rm cms} \vert < 3.53$ and is smaller than unity by 3.7$\sigma$ in $2.5<p_{\rm T}<3.5$ GeV/$c$. The data are described by model calculations including cold nuclear matter effects.
The production cross sections of the prompt charmed mesons ${\rm D^0}$, ${\rm D^+}$, ${\rm D^{*+}}$ and ${\rm D_s^+}$ were measured at mid-rapidity in proton-proton collisions at a centre-of-mass ...energy $\sqrt{s}=7$ TeV with the ALICE detector at the Large Hadron Collider (LHC). D mesons were reconstructed from their decays ${\rm D}^0 \to {\rm K}^-\pi^+$, ${\rm D}^+\to {\rm K}^-\pi^+\pi^+$, ${\rm D}^{*+} \to {\rm D}^0 \pi^+$, ${\rm D_s^{+}\to \phi\pi^+\to K^-K^+\pi^+}$, and their charge conjugates. With respect to previous measurements in the same rapidity region, the coverage in transverse momentum ($p_{\rm T}$) is extended and the uncertainties are reduced by a factor of about two. The accuracy on the estimated total $\rm c\overline c$ production cross section is likewise improved. The measured $p_{\rm T}$-differential cross sections are compared with the results of three perturbative QCD calculations.
The inclusive J/$\psi$ production has been studied in Pn-Pb and pp collisions at the centre-of-mass energy per nucleon pair $\sqrt{s_{\rm NN}}=5.02$ TeV, using the ALICE detector at the CERN LHC. The ...J/$\psi$ meson is reconstructed, in the centre-of-mass rapidity interval $2.5<y<4$ and in the transverse-momentum range $p_{\rm T}<12$ GeV/$c$, via its decay to a muon pair. In this Letter, we present results on the inclusive J/$\psi$ cross section in pp collisions at $\sqrt{s}=5.02$ TeV and on the nuclear modification factor $R_{\rm AA}$. The latter is presented as a function of the centrality of the collision and, for central collisions, as a function of the transverse momentum $p_{\rm T}$ of the J/$\psi$. The measured $R_{\rm AA}$ values indicate a suppression of the J/$\psi$ in nuclear collisions and are then compared to our previous results obtained in Pb-Pb collisions at $\sqrt{s_{\rm NN}}=2.76$ TeV. The ratio of the $R_{\rm AA}$ values at the two energies is also computed and compared to calculations of statistical and dynamical models. The numerical value of the ratio for central events (0-10\% centrality) is $1.17 \pm 0.04 {\rm{(stat)}}\pm 0.20 {\rm{(syst)}}$. In central events, as a function of $p_{\rm T}$, a slight increase of $R_{\rm AA}$ with collision energy is visible in the region $2<p_{\rm T}<6$ GeV/$c$. Theoretical calculations provide a good description of the measurements, within uncertainties.
The production of beauty hadrons was measured via semi-leptonic decays at mid-rapidity with the ALICE detector at the LHC in the transverse momentum interval $1<p_{\rm T}<8$ GeV/$c$ in minimum-bias ...p-Pb collisions at $\sqrt{s_{\rm NN}}=5.02$ TeV and in $1.3<p_{\rm T}<8$ GeV/$c$ in the 20% most central Pb-Pb collisions at $\sqrt{s_{\rm NN}}=2.76$ TeV. The pp reference spectra at $\sqrt{s}=5.02$ TeV and $\sqrt{s}=2.76$ TeV, needed for the calculation of the nuclear modification factors $R_{\rm pPb}$ and $R_{\rm PbPb}$, were obtained by a pQCD-driven scaling of the cross section of electrons from beauty-hadron decays measured at $\sqrt{s}=7$ TeV. The $R_{\rm PbPb}$ is about 0.7 with an uncertainty of about 30% in the interval $3<p_{\rm T}<6$ GeV/$c$ and 0.47 with an uncertainty of 25% in $6<p_{\rm T}<8$ GeV/$c$ in Pb-Pb collisions. Below $p_{\rm T}=3$ GeV/$c$, the $R_{\rm PbPb}$ values increase with decreasing transverse momentum with systematic uncertainties of 30-45%. The $R_{\rm pPb}$ is consistent with unity within systematic uncertainties of about 20% at high $p_{\rm T}$, increasing at low $p_{\rm T}$, and is well described by theoretical calculations that include cold nuclear matter effects in p-Pb collisions. The measured $R_{\rm pPb}$ and these calculations indicate that cold nuclear matter effects are small at high transverse momentum also in Pb-Pb collisions. Therefore, the observed reduction of $R_{\rm PbPb}$ below unity for high $p_{\rm T}$ can be ascribed to an effect of the hot and dense medium formed in Pb-Pb collisions.
A detailed study of pseudorapidity densities and multiplicity distributions of primary charged particles produced in proton-proton collisions, at $\sqrt{s} =$ 0.9, 2.36, 2.76, 7 and 8 TeV, in the ...pseudorapidity range $|\eta|<2$, was carried out using the ALICE detector. Measurements were obtained for three event classes: inelastic, non-single diffractive and events with at least one charged particle in the pseudorapidity interval $|\eta|<1$. The use of an improved track-counting algorithm combined with ALICE's measurements of diffractive processes allows a higher precision compared to our previous publications. A KNO scaling study was performed in the pseudorapidity intervals $|\eta|<$ 0.5, 1.0 and 1.5. The data are compared to other experimental results and to models as implemented in Monte Carlo event generators PHOJET and recent tunes of PYTHIA6, PYTHIA8 and EPOS.
The production of charged pions, kaons and (anti)protons has been measured at mid-rapidity ($-0.5 < y < 0$) in p-Pb collisions at $\sqrt{s_{\rm NN}} = 5.02$ TeV using the ALICE detector at the LHC. ...Exploiting particle identification capabilities at high transverse momentum ($p_{\rm T}$), the previously published $p_{\rm T}$ spectra have been extended to include measurements up to 20 GeV/$c$ for seven multiplicity event classes. The $p_{\rm T}$ spectra for pp collisions at $\sqrt{s}=7$ TeV, needed to interpolate a pp reference spectrum, have also been extended up to 20 GeV/$c$ to measure the nuclear modification factor ($R_{\rm pPb}$) in non-single diffractive p-Pb collisions. At intermediate transverse momentum ($2 < p_{\rm T} < 10$ GeV/$c$) the proton-to-pion ratio increases with multiplicity in p-Pb collisions, a similar effect is not present in the kaon-to-pion ratio. The $p_{\rm T}$ dependent structure of such increase is qualitatively similar to those observed in pp and heavy-ion collisions. At high $p_{\rm T}$ ($>10$ GeV/$c$), the particle ratios are consistent with those reported for pp and Pb-Pb collisions at the LHC energies. At intermediate $p_{\rm T}$ the (anti)proton $R_{\rm pPb}$ shows a Cronin-like enhancement, while pions and kaons show little or no nuclear modification. At high $p_{\rm T}$ the charged pion, kaon and (anti)proton $R_{\rm pPb}$ are consistent with unity within statistical and systematic uncertainties.
The elliptic flow of electrons from heavy-flavour hadron decays at mid-rapidity ($|y|$ $<$ 0.7) is measured in Pb-Pb collisions at $\sqrt{s_{\rm NN}} = 2.76$ TeV with ALICE at the LHC. The particle ...azimuthal distribution with respect to the reaction plane can be parametrized with a Fourier expansion, where the second coefficient ($v_{\rm 2}$) represents the elliptic flow. The $v_{\rm 2}$ coefficient of inclusive electrons is measured in three centrality classes (0-10%, 10-20% and 20-40%) with the event plane and the scalar product methods in the transverse momentum ($p_{\rm T}$) intervals 0.5-13 GeV/$c$ and 0.5-8 GeV/$c$, respectively. After subtracting the background, mainly from photon conversions and Dalitz decays of neutral mesons, a positive $v_{\rm 2}$ of electrons from heavy-flavour hadron decays is observed in all centrality classes, with a maximum significance of $5.9\sigma$ in the interval $2 <$ $p_{\rm T}$ $<$ 2.5 GeV/$c$ in semi-central collisions (20-40%). The value of $v_{\rm 2}$ decreases towards more central collisions at low and intermediate $p_{\rm T}$ (0.5 $<$ $p_{\rm T}$ $<$ 3 GeV/$c$). The $v_{\rm 2}$ of electrons from heavy-flavour hadron decays at mid-rapidity is found to be similar to the one of muons from heavy-flavour hadron decays at forward rapidity (2.5 $<$ $y$ $<$ 4). The results are described within uncertainties by model calculations including substantial elastic interactions of heavy quarks with an expanding strongly-interacting medium.
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