Iron is essential for life. Many enzymes require iron for appropriate function. However, dysregulation of intracellular iron homeostasis produces excessive reactive oxygen species (ROS) via the ...Fenton reaction and causes devastating effects on cells, leading to ferroptosis, an iron-dependent cell death. In order to protect against harmful effects, the intracellular system regulates cellular iron levels through iron regulatory mechanisms, including hepcidin-ferroportin, divalent metal transporter 1 (DMT1)-transferrin, and ferritin-nuclear receptor coactivator 4 (NCOA4). During iron deficiency, DMT1-transferrin and ferritin-NCOA4 systems increase intracellular iron levels via endosomes and ferritinophagy, respectively. In contrast, repleting extracellular iron promotes cellular iron absorption through the hepcidin-ferroportin axis. These processes are regulated by the iron-regulatory protein (IRP)/iron-responsive element (IRE) system and nuclear factor erythroid 2-related factor 2 (Nrf2). Meanwhile, excessive ROS also promotes neuroinflammation by activating the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). NF-κB forms inflammasomes, inhibits silent information regulator 2-related enzyme 1 (SIRT1), and induces pro-inflammatory cytokines (IL-6, TNF-α, and IL-1β). Furthermore, 4-hydroxy-2,3-
-nonenal (4-HNE), the end-product of ferroptosis, promotes the inflammatory response by producing amyloid-beta (Aβ) fibrils and neurofibrillary tangles in Alzheimer's disease, and alpha-synuclein aggregation in Parkinson's disease. This interplay shows that intracellular iron homeostasis is vital to maintain inflammatory homeostasis. Here, we review the role of iron homeostasis in inflammation based on recent findings.
Escherichia coli O157:H7 and Shigella flexneri are well-known food-borne pathogens causing severe food poisoning at low infectious doses. Bacteriophages have been approved for food applications by ...the US Food and Drug Administration (FDA) and have been suggested as natural food preservatives to control specific food-borne pathogens. To develop a novel natural food preservative against E. coli O157:H7 and S. flexneri, a new bacteriophage needs to be isolated and characterized.
Bacteriophage HY01 infecting both E. coli O157:H7 and S. flexneri was isolated from a swine fecal sample. HY01 belongs to the family Myoviridae and is stable under various temperature and pH conditions. One-step growth curve analysis showed relatively short eclipse and latent periods as well as large burst size. The 167-kb genome sequence of HY01 was sequenced, and a comparative genome analysis with T4 for non-O157:H7 E. coli suggests that the receptor recognition protein of HY01 plays an important role in determination of host recognition and specificity. In addition, food applications using edible cabbage were conducted with two E. coli O157:H7 strains (ATCC 43890 and ATCC 43895), showing that treatment with HY01 inhibits these clinical and food isolates with >2 log reductions in bacterial load during the first 2 h of incubation.
HY01 can inhibit both E. coli O157:H7 and S. flexneri with large burst size and stability under stress conditions. The ability of HY01 to infect both E. coli O157:H7 and S. flexneri may be derived from the presence of two different host specificity-associated tail genes in the genome. Food applications revealed the specific ability of HY01 to inhibit both pathogens in food, suggesting its potential as a novel biocontrol agent or novel natural food preservative against E. coli O157:H7 and potentially S. flexneri.
Exceptional challenges have confronted the rational design of colorimetric sensors for saturated aliphatic hydrocarbons (SAHCs). The main reasons for this difficulty are the extremely nonpolar nature ...of these targets and their lack of functional groups that can interact with probes. By taking advantage of a mechanochromic conjugated polydiacetylene (PDA) and the hydrocarbon‐induced swelling properties of polydimethylsiloxane (PDMS), a sensor film that enables simple, colorimetric differentiation between a variety of C5 to C14 aliphatic hydrocarbons is fabricated. The unprecedented PDA–PDMS composite sensor undergoes a blue‐to‐red colorimetric transition on a timescale that is dependent on the chain length of the hydrocarbon target. In addition, the development of the red color is directly proportional to the swelling ratio of the film. This straightforward approach enables naked‐eye differentiation between n‐pentane and n‐heptane. The versatility of the sensor system is demonstrated by using it for the colorimetric determination of kerosene in adulterated diesel oil. Finally, the observation that a PDA microcrystal in the film undergoes significant expansion and tearing in concert with a blue‐to‐red colorimetric transition during the swelling process provides direct evidence for the mechanism for the mechanochromic behavior of the PDA.
By taking advantage of a mechanochromic conjugated polydiacetylene (PDA) and the hydrocarbon‐induced swelling properties of polydimethylsiloxane (PDMS), a sensor film that enables colorimetric differentiation among saturated aliphatic hydrocarbons (SAHCs) is developed. The unprecedented PDA–PDMS composite sensor undergoes a blue‐to‐red colorimetric transition that is dependent on the chain length of the hydrocarbon target.
Vehicle-IT convergence technology is a rapidly rising paradigm of modern vehicles, in which an electronic control unit (ECU) is used to control the vehicle electrical systems, and the controller area ...network (CAN), an in-vehicle network, is commonly used to construct an efficient network of ECUs. Unfortunately, security issues have not been treated properly in CAN, although CAN control messages could be life-critical. With the appearance of the connected car environment, in-vehicle networks (e.g., CAN) are now connected to external networks (e.g., 3G/4G mobile networks), enabling an adversary to perform a long-range wireless attack using CAN vulnerabilities. In this paper we show that a long-range wireless attack is physically possible using a real vehicle and malicious smartphone application in a connected car environment. We also propose a security protocol for CAN as a countermeasure designed in accordance with current CAN specifications. We evaluate the feasibility of the proposed security protocol using CANoe software and a DSP-F28335 microcontroller. Our results show that the proposed security protocol is more efficient than existing security protocols with respect to authentication delay and communication load.
Blood continually contributes to the maintenance of homeostasis of the body and contains information regarding the health state of an individual. However, current hematological analyses predominantly ...rely on a limited number of CD markers and morphological analysis. In this work, differentially sensitive fluorescent compounds based on TCF scaffolds are introduced that are designed for fluorescent phenotyping of blood. Depending on their structures, TCF compounds displayed varied responses to reactive oxygen species, biothiols, redox‐related biomolecules, and hemoglobin, which are the primary influential factors within blood. Contrary to conventional CD marker‐based analysis, this unbiased fluorescent phenotyping method produces diverse fingerprints of the health state. Precise discrimination of blood samples from 37 mice was demonstrated based on their developmental stages, ranging from 10 to 19 weeks of age. Additionally, this fluorescent phenotyping method enabled the differentiation between drugs with distinct targets, serving as a simple yet potent tool for pharmacological analysis to understand the mode of action of various drugs.
A differential sensing approach is demonstrated to distinguish distinct aging status of mouse using TCF probes. Despite there is no reliable CD markers selective for aging progress, cross‐reactivity of TCF probes generates unique fingerprint of aging by blood cell analysis.
Although carbon nanotubes (CNTs) are used to reinforce aluminum alloys, the underlying strengthening mechanisms have not yet been clarified. Here, we focus on elucidating several of these mechanisms ...including load transfer, generation of dislocations from thermal mismatch, and the Orowan looping system to explain the enhanced mechanical properties of CNT-reinforced Al. The sample preparation procedure involves mechanically pulverizing Al in paraffin oil mixed with CNTs dispersed in dichloroethane. The mixture was then sintered and melt-blended to produce solid samples. The formation of covalent bonds (Al4C3) between Al and CNT walls was confirmed by X-ray diffraction, Raman spectroscopy, and transmission electron microscope measurements. The yield strength and tensile strength were improved by 60% and 23%, respectively, with the addition of 0.2 wt% CNTs. These enhancements corroborate well with the predicted values from the multiple strengthening mechanisms.
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