Cord blood is a rich source of primitive hemopoietic stem cells. In clinical hematology it is transplanted instead of bone marrow or peripheral blood stem cells. The Institute of Hematology and Blood ...Transfusion in Warsaw has had a Cord Blood Bank (WACB) since 1997. WACB collaborates with Eurocord Transplant Group and with Bone Marrow Donors Worldwide transfering data on frozen CB intended for transplantation. During 1997–2000, a total of 159 unrelated and 29 related cord blood units were collected. More than 70% of transplants of CB were perfomed in pediatric recipiens it is necessary to reduce its volume of storage of CB units. Separation techniques reduce sample volume to ±20 ml. Sedimentation methods reduce the number of RBC to be infused. RBC depletion reduces the risk of incompatibile reaction. Sedimentation reduces side-effects of the DMSO (dimethyl sulfoxide) cytotoxity (DMSO volume is reduced to 4 ml).
The aim of our study was to evaluate methods of isolating leukocytes from cord blood within a closed system. Two methods of isolation have been tested: 6% hydroxyethyl starch in 0,9% NaCI and 3% gelatin in 0,9% NaCI (Gelafundine, Braun). Centrifugation and sedimentation methods have been used. The final volume of cord blood was 20 ml for each unit. The best results were obtained for sedimentation. With 3% gelatin sedimentation 75,1% WBC and 81,3% CD34+ were recovered, while the waste of RBC was 97,2%; with 6% HES sedimentation the results were: 65%; 90,3%, and 80,5% respectively. The results of our centrifugation methods revealed a great loss of progenitor cells (approx. 40%). 6% HES and 3% gelatin are approved for clinical use, therefore sedimentation methods based on these media are safe for recipients. Furthermore, the use of closed system recommended by Eurocord Transplant, prevents bacterial contamination.
•Coloured potatoes contained 3-times higher of total phenolic content than yellow-fleshed.•The predominating phenolic acids in potato were chlorogenic acid and its isomers.•The production process of ...dried potato dice affects the content of phenolic acids.•Dried potato dice obtained from yellow-fleshed potatoes did not contain of phenolic acids.•Coloured dried potato dice contained about 4% of total phenolic content of the raw material.
The purpose of the research was to examine the effect of the laboratory production of dried potato dice on the content of phenolic compounds in one yellow-fleshed potato variety and four blue-fleshed potatoes varieties. Coloured-flesh potato varieties were characterised by about three times higher amount of total phenolic content than traditional yellow-fleshed ones. The predominating phenolic acids in potato were chlorogenic acid and its isomers, which account about 90% of total phenolic content in tubers. The phenolic acid content decreased by 80% after peeling the blue-fleshed potatoes and by 60% after peeling the yellow variety. The dried potato dice obtained from yellow-fleshed potatoes had no content of phenolic acids but produced from colour-fleshed potatoes contained about 4% of the original phenolic content of the raw material. Chlorogenic acid amounted about 97% of total phenolic acid content, and the rest was neochlorogenic acid.
Background and Objectives
The competent authority (CA) responsible for external inspections of Polish blood establishments (BEs) and supervision of the quality system is the Institute of Haematology ...and Transfusion Medicine (IHTM). Before the implementation of the European Blood Inspection System (EuBIS) classification of non‐compliance, the IHTM inspections were conducted according to national guidelines and the non‐compliance‐related recommendations were based on the inspectors' own experience and interpretation of the observed problems. Since 2009, IHTM inspections were already performed according to EuBIS guidelines. The study assessed the impact of the EuBIS classification on the IHTM recommendations. We assumed that the implementation of consistent assessment criteria contributed to the upgrading of the quality of BE inspections.
Materials and Methods
BE‐inspection protocols; 30 from 2009 to 2010 and 61 from 2016 to 2019. Non‐compliance‐related recommendations were classified according to the seriousness of non‐compliances (critical, major, other significant, and observation) and also to the area of BE activity (documentation, organisation of work, qualification and validation, pathway from donor qualification to blood component‐issue, quality control of blood components, adverse events and reactions).
Results
The recommendations mostly referred to document‐keeping and work organisation and were distributed as follows: 2009–2 critical (others unclassified), 2010–1‐13 major, 4–25 other significant and 1–7 suggestions, 2016–2019–3‐9 critical, 90–196 major, and 157–297 other significant as well as 14–22 suggestions.
Conclusion
Polish BEs still require: integrated document management, analysis of IHTM recommendations, implementation of corrective and preventive measures and personnel training in identifying similar non‐compliances in other procedures.
