•DPSIR theory is used to analyze the coupling coordination mechanism among systems.•An evaluation index system of coupling coordinated development is proposed.•A CCD evaluation model optimized by ...information entropy is further constructed.•The characteristics of CCD in time and spatial dimension are analyzed.•Optimal strategies for the provinces in the YREB are put forward.
The interaction between regional energy, economy and ecology system has the characteristics of high correlation and complexity. In-depth study of the relationship between the three systems is of great significance for regional sustainable development. In this paper, DPSIR (driving forces, pressures, states, impacts and responses) theory is innovatively introduced to analyze the coupling coordination mechanism among systems. On this basis, an evaluation system for the coupling coordination development of regional energy-economy-ecology system is proposed. A coupling coordination degree (CCD) evaluation model optimized by information entropy is further constructed, and the panel data of 11 provinces along the Yangtze River Economic Belt (YREB) from 2008 to 2017 are used for empirical analysis of this region. The results show that the development of the energy and economy systems in the YREB has strong similarities, while the evaluation values of ecology systems in the provinces mainly fluctuate or first descend then rise. The development of the ecology system is influenced by the development of energy system and economy system on the one hand, and also by regional policies on the other hand. The CCD on the time dimension is given priority to with fluctuations rise development momentum, on the spatial dimension is roughly east high and west low development pattern. And the main factors restricting the coupling coordinated development of the YREB are different in the east, central and west. The three eastern provinces all belong to the type of ecology lag, while the four provinces in the central region belong to economy lagged. The development of energy and economy systems in the four western provinces lags behind their ecology systems. In addition, this paper proposes optimal strategies for the provinces, in which the middle and upper reaches of the Yangtze River should reduce energy consumption per unit of GDP and accelerate the transformation from traditional economy to green economy. The downstream region should improve the ecological carrying capacity of the resources and environment, and promote the economic conversion rate of scientific and technological achievements. The research process and results show that the combination of DPSIR theory and CCD model can provide a general tool for determining the coupling coordinated effect between subsystems.
Hemiptera, the largest non-holometabolous order of insects, represents approximately 7% of metazoan diversity. With extraordinary life histories and highly specialized morphological adaptations, ...hemipterans have exploited diverse habitats and food sources through approximately 300 Myr of evolution. To elucidate the phylogeny and evolutionary history of Hemiptera, we carried out the most comprehensive mitogenomics analysis on the richest taxon sampling to date covering all the suborders and infraorders, including 34 newly sequenced and 94 published mitogenomes. With optimized branch length and sequence heterogeneity, Bayesian analyses using a site-heterogeneous mixture model resolved the higher-level hemipteran phylogeny as (Sternorrhyncha, (Auchenorrhyncha, (Coleorrhyncha, Heteroptera))). Ancestral character state reconstruction and divergence time estimation suggest that the success of true bugs (Heteroptera) is probably due to angiosperm coevolution, but key adaptive innovations (e.g. prognathous mouthpart, predatory behaviour, and haemelytron) facilitated multiple independent shifts among diverse feeding habits and multiple independent colonizations of aquatic habitats.
•Analyze the synergy of CO2 emission factors of energy-intensive industries via EMD.•Synergy of CO2 emission factors: long-term trend, short-term volatility.•Elastic coefficient model is used to ...reveal the heterogeneity of the 10 factors.•Six industries are divided into “stable and balanced” and “sensitive and uneven”.
Carbon emission is a global problem that countries around the world are paying attention to and urgently need to be resolved. Under the requirements of China's high-quality economic development and the carbon peaking and carbon neutrality goals, energy-intensive industries, as a key link in the industrial chain and the main source of China's CO2 emissions, have attracted much attention. This paper made a statistical analysis of the scale development of six energy-intensive industries, and explored the fluctuation characteristics of their carbon emissions. The empirical mode decomposition (EMD) model was further introduced to analyze the and synergistic effect among the driving factors of carbon emission, revealing the synergistic mechanism of long-term trend and short-term volatility of factors. Then, the elastic sensitivity analysis model of carbon emissions in energy-intensive industries was constructed to study the heterogeneity of driving effects of factor indicators. From the perspective of factors driving, this article reveals the heterogeneity of the driving effects of ten key factor indicators including economic level (GDP), urbanization level (UP), industrial structure (IS), technological innovation (TI), and environmental regulation (ER); from the perspective of industry development, this paper summarized the “stable and balanced” and “sensitive and uneven” trends presented by the heterogeneity of the six energy-intensive industries. Combining the characteristics of industry emission fluctuations, driving factors’ synergies and heterogeneity, from the perspective of short-term and long-term development, four targeted emission reduction control strategies were proposed to provide reference for the low-carbon development of energy-intensive industries.
In our previous research, a petroleum degrading bacteria strain Bacillus licheniformis Y-1 was obtained in Dagang Oilfield which had the capability of producing biosurfactant. This biosurfactant was ...isolated and purified in this work. The biosurfactant produced by strain Y-1 had the capability to decrease the surface tension of water from 74.66 to 27.26mN/m, with the critical micelle concentration (CMC) of 40mg/L. The biosurfactant performed not only excellent stabilities against pH, temperature and salinity, but also great emulsifying activities to different kinds of oil, especially the crude oil. According to the results of FT-IR spectrum and 1H NMR spectrum detection, the surfactant was determined to be a cyclic lipopeptide. Furthermore, through the addition of surfactant, the effect of petroleum contaminated soil remediation by fungi got a significant improvement.
•A biosurfactant produced by a petroleum degrading bacteria strain Bacillus licheniformis Y-1 was isolated and purified.•This biosurfactant was determined to have excellent emulsifying activity.•By using FT-IR and 1H NMR, the structure of this biosurfactant was determined to be a cyclic lipopeptide.•Through the addition of this biosurfactant, the effect of petroleum contaminated soil remediation by fungi got a significant improvement.
Blockade of programmed death-ligand 1 (PD-L1) by therapeutic antibodies has shown to be a promising strategy in cancer therapy, yet clinical response in many types of cancer, including prostate ...cancer (PCa), is limited. Tumor cells secrete PD-L1 through exosomes or splice variants, which has been described as a new mechanism for the resistance to PD-L1 blockade therapy in multiple cancers, including PCa. This suggests that cutting off the secretion or expression of PD-L1 might improve the response rate of PD-L1 blockade therapy in PCa treatment. Here we report that p300/CBP inhibition by a small molecule p300/CBP inhibitor dramatically enhanced the efficacy of PD-L1 blockade treatment in a syngeneic model of PCa by blocking both the intrinsic and IFN-γ-induced PD-L1 expression. Mechanistically, p300/CBP could be recruited to the promoter of CD274 (encoding PD-L1) by the transcription factor IRF-1, which induced the acetylation of Histone H3 at CD274 promoter followed by the transcription of CD274. A485, a p300/CBP inhibitor, abrogated this process and cut off the secretion of exosomal PD-L1 by blocking the transcription of CD274, which combined with the anti-PD-L1 antibody to reactivate T cells function for tumor attack. This finding reports a new mechanism of how cancer cells regulate PD-L1 expression through epigenetic factors and provides a novel therapeutic approach to enhance the efficacy of immune checkpoint inhibitors treatment.
In this work, a hydrocarbon-degrading bacterium Y-1 isolated from petroleum contaminated soil in the Dagang Oilfield was investigated for its potential effect in biodegradation of crude oil. ...According to the analysis of 16S rRNA sequences, strain Y-1 was identified as Bacillus licheniformis. The growth parameters such as pH, temperature, and salinity were optimised and 60.2% degradation of crude oil removal was observed in 5days. The strain Y-1 showed strong tolerance to high salinity, alkalinity, and temperature. Emplastic produced by strain Y-1 at high temperatures could be applied as biosurfactant. Gas chromatography analysis demonstrated that the strain Y-1 efficiently degraded different alkanes from crude oil, and the emplastic produced by strain Y-1 promoted the degradation rates of long-chain alkanes when the temperature increased to 55°C. Therefore, strain Y-1 would play an important role in the area of crude oil contaminant bioremediation even in some extreme conditions.
•A bacillus licheniformis strain Y-1 was isolated, and the crude oil degradation rate of 60.2% was got in only 5days.•Strain Y-1 showed strong tolerance to high salinity, alkalinity, and temperature.•Degradation of both short-chain alkanes and long-chain alkanes were obvious.•Emplastic produced by strain Y-1 at high temperature intensified the emulsifying effect.
A magnetic metal-organic framework nanocomposite (magnetic MIL-68(Ga)) was synthesized through a "one pot" reaction and used for heavy metal ion removal. The morphology and elemental properties of ...the nanocomposite were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR), X-ray powder diffraction (XRD), as well as zeta potential. Moreover, the factors affecting the adsorption capacity of the nanocomposite, including time, pH, metal ion type and concentration, were studied. It was found that the adsorption capacity of magnetic MIL-68(Ga) for Pb
and Cu
was 220 and 130 mg/g, respectively. Notably, the magnetic adsorbents could be separated easily using an external magnetic field, regenerated by ethylenediaminetetraacetic acid disodium salt (EDTA-Na
) and reused three times, in favor of practical application. This study provides a reference for the rapid separation and purification of heavy metal ions from wastewater.
We present SeqOthello, an ultra-fast and memory-efficient indexing structure to support arbitrary sequence query against large collections of RNA-seq experiments. It takes SeqOthello only 5 min and ...19.1 GB memory to conduct a global survey of 11,658 fusion events against 10,113 TCGA Pan-Cancer RNA-seq datasets. The query recovers 92.7% of tier-1 fusions curated by TCGA Fusion Gene Database and reveals 270 novel occurrences, all of which are present as tumor-specific. By providing a reference-free, alignment-free, and parameter-free sequence search system, SeqOthello will enable large-scale integrative studies using sequence-level data, an undertaking not previously practicable for many individual labs.
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•Transcription profiling reveals the role of sulfur metabolism in heavy metal capture.•A sulfur metabolism operon is de novo synthesized for constructing synthetic cells.•The ...synthesized operon remodels sulfur metabolism network in large scale.•The synthesized operon remarkably enhances heavy metal-tolerating capacity.•The synthesized operon remarkably promotes heavy metal capture in synthetic cells.
Heavy metal contamination is becoming a global problem threatening human health. Heavy metal removal by engineered microbes by cellular adsorption and uptake is a promising strategy for treatment of heavy metal contamination. However, this strategy is confronted with limited heavy metal-capturing elements. In this study, we performed a transcription profiling-guided strategy for construction of heavy metal-capturing synthetic bacteria. Transcription profiling of a heavy metal-tolerating Cupriavidus taiwanensis strain revealed up-regulation of sulfur metabolism-related operons (e.g., iscSAU and moaEDAB) by Pb2+ and Cd2+. A synthetic Escherichia coli strain, EcSSMO, was constructed by design of a synthetic sulfur metabolism operon (SSMO) based on iscSAU/moaEDAB. Biochemical analysis and X-ray photoelectron spectroscopy (XPS) revealed that the synthetic bacteria had remodeled sulfur metabolism and enhanced heavy metal-tolerating capacity, with higher surviving EcSSMO cells than the surviving control cells Ec0 (not containing SSMO) at 50 mg/L of Pb2+ and Cd2+ (>92 % versus <10 %). Moreover, EcSSMO exhibited much higher heavy metal-capturing capacity than Ec0, removing>90 % of Pb2+ and Cd2+ at 5 mg/L of Pb2+ and Cd2+, and >40 % of both heavy metals even at 50 mg/L of Pb2+ and Cd2+. This study reveals emphasizes feasibility of transcription profiling-guided construction of synthetic organisms by large-scale remodeling metabolic network.
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