An integrated geochemical, morphological and structural analysis was applied to a basin filled with clayey sediments in southern Italy (Ofanto Valley) to delineate tectonic features. More than 100 ...soil-gas samples were collected and analysed for CO
2, Rn and He, and the resulting distribution was compared with the location and orientation of field-observed brittle deformations (faults and fractures), and air-photo interpreted morphotectonic features. The results show that the highest helium, radon and CO
2 values occur preferentially along elongated zones similar to the most representative trends obtained by geomorphological and mesostructural analyses, i.e. anti-Apennine, Apennine and, secondarily, N–S orientations. Furthermore, the development of geostatistical techniques has allowed the semi-quantitative evaluation of the anisotropic soil-gas distribution. The gas-distribution pattern is considered to result from the combination of the anisotropic distribution of fracture traces and the randomly distributed background field. The correspondence between soil-gas distribution and geomorphological/mesostructural features, as well as the results from the geostatistical analysis, suggest that gas leakage towards the surface is controlled by the same structural pattern which also created some morphological features. This technique has been shown to be a useful tool for neotectonic studies; this is especially true in basins filled with clayey sediments, as soil gas is even able to define the leakage of deep-seated gases along tectonic discontinuities which have no surface expression.
Extubation failure (EF) refers to the inability to maintain spontaneous breathing after removal of endotracheal tube. The aim of this review is to identify the best parameter to predict EF in adult ...intensive care patients.
We searched for publications in PubMed (2000-2016). Studies of patients intubated and mechanically ventilated for more than 24 hours were included and divided in groups basing on the extubation method. 2x2 tables were performed to evaluate the sensitivity, specificity and the predictive values only for those parameters investigated in more than three studies. Studies were divided in groups, basing on time required to define EF (<24 hours, <72 or >72 hours), and EF percentage was calculated for each group.
On 443 potentially studies, 26 were included. Rapid Shallow Breathing Index (RSBI) and cough strength parameters were found in more than three studies. RSBI or cough strength parameter showed a sensitivity of 20-88.8% or 55.5-85.2%, a specificity of 68.5-94.8% or 24-49%, a positive predictive value (PPV) of 39.5-66.6% or 24-49% and a negative predictive value of 98-82% or 89.5-96.4%, respectively. EF rate was 12.5%, 15.3% and 22% in patients evaluated within 24 hours, 72 hours and over 72 hours, respectively.
This review shows that all parameters used to predict EF have a low PPV. Therefore, the limitation of use of such predictive tests may prolong unnecessarily the intubation and increase the unfavorable outcome. A prospective study involving all variables could be useful to predict the EF in ICU.
Although CO2 capture and storage in deep, offshore reservoirs is a proven technology, as illustrated by over 15 years of operation of the Sleipner site in the Norwegian North Sea, potential leakage ...from such sites into the overlying water column remains a concern for some stakeholders. Therefore, we are obliged to carefully assess our ability to predict and monitor the migration, fate, and potential ecosystem impact of any leaked CO2. The release of bubbles from the sea floor, their upward movement, and their dissolution into the surrounding water controls the initial boundary conditions, and thus an understanding of the behavior of CO2 bubbles is critical to address such issues related to monitoring and risk assessment. The present study describes results from an in situ experiment conducted in 12 m deep marine water near the extinct volcanic island of Panarea (Italy). Bubbles of a controlled size were created using natural CO2 released from the sea floor, and their evolution during ascent in the water column was monitored via both video and chemical measurements. The obtained results were modelled and a good fit was obtained, showing the potential of the model as a predictive tool. These preliminary results and an assessment of the difficulties encountered are examined and will be used to improve experimental design for the subsequent phase of this research.
Monitoring of the water column in the vicinity of offshore Carbon Capture and Storage (CCS) sites is needed to ensure site integrity and to protect the surrounding marine ecosystem. In this regard, ...the use of continuous, autonomous systems is considered greatly advantageous due to the costs and limitations of periodic, ship-based sampling campaigns. While various geochemical monitoring tools have been developed their elevated costs and complexities mean that typically only one unit can be deployed at a time, yielding single point temporal data but no spatial data. To address this the authors have developed low-cost pCO2 sensors (GasPro-pCO2) that are small, robust, stable, and which have a low power consumption, characteristics which allow for the deployment of numerous units to monitor the spatial-temporal distribution of pCO2, temperature, and water pressure in surface water environments. The present article details the results of three field deployments at the natural, CO2-leaking site near Panarea, Island. While the first consisted of 6 probes placed on the sea floor for a 2.5 month period, the other two involved the deployment of 20 GasPro units along a transect through the water column in the vicinity of active CO2 seeps over 2 – 4 days. Results show both transport and mixing processes and highlight the dynamic nature of the leakage-induced marine geochemical anomalies. Implications for monitoring programs as well as potential impacts are discussed.
G protein-coupled receptors (GPCR) play a crucial role in the regulation of the immune response by, e.g., chemokines, PGs, and beta(2)-adrenergic agonists. The responsiveness of these GPCRs is turned ...off by the family of G protein-coupled receptor kinases (GRK1-6). These kinases act by phosphorylating the GPCR in an agonist-dependent manner, resulting in homologous desensitization of the receptor. Although GRKs are widely expressed throughout the body, leukocytes express relatively high levels of GRKs, in particular GRK2, -3, and -6. We investigated whether in vivo the inflammatory disease adjuvant arthritis (AA) induces changes in GRK expression and function in the immune system. In addition, we analyzed whether the systemic effects of AA also involve changes in GRKs in nonimmune organs. At the peak of the inflammatory process, we observed a profound down-regulation of GRK2, -3, and -6 in splenocytes and mesenteric lymph node cells from AA rats. Interestingly, no changes in GRK were observed in thymocytes and in nonimmune organs such as heart and pituitary. During the remission phase of AA, GRK levels in spleen and mesenteric lymph nodes are returning to baseline levels. The decrease in GRK2 at the peak of AA is restricted to CD45RA(+) B cells and CD4(+) T cells, and was not observed in CD8(+) T cells. In conclusion, we demonstrate in this study, for the first time, that an inflammatory process in vivo induces a tissue-specific down-regulation of GRKs in the immune system.
As stated in The Lancet Public Health editorial, "No public health without planetary health", the future health of the planet and human health are inextricably linked. For this reason, global ...citizens, practitioners, and professionals, especially those involved in Public Health, must be equipped to address and understand the field of Planetary Health (PH), which looks at the complex connections associated with disruptions to natural systems and resulting impacts on human health. Strategies aimed at incorporating PH education into high schools and academic curricula are required to build capacity for future national and local PH leadership. One of the most relevant tools used to achieve these goals is the PH Education Framework, designed by the Planetary Health Alliance (PHA). This framework considers five foundational domains as essential for PH knowledge, values, and practice, and has been currently applied by different institutions involved in PH. The Italian Institute for PH (IIPH) applied this framework to a school-based project for education in urban health, which will be better described in the workshop presentation. The PH Education Framework domains were used for the development of four interactive sessions oriented to raise students' interest on the topic and stimulate active participation during and after the intervention, also with simple pro-environmental behaviours. The experience was shown to be fruitful for Public Health residents as well, who were deeply involved in and led the interactive sessions held in the high school. By means of a qualitative assessment, residents demonstrated to have increased their self-confidence, knowledge and leadership skills in PH. Further research and applications of the PH Education Framework are needed in order to increase evidence and awareness in PH and strengthen PH collaboration in Europe and beyond.
Recent evidence indicates that Chlamydophila psittaci (Cp) may establish chronic infections, which may promote autoimmunity and/or B cell lymphoproliferation.
The presence of a subclinical Cp ...infection was investigated in 293 patients with chronic inflammatory polyarthritis, including 175 patients with rheumatoid factor (RF)-positive and/or anti-CCP-positive rheumatoid arthritis (RA) and 118 with seronegative polyarthritis (46 RF-negative/anti-CCP-negative RA, 36 psoriatic arthritis and 36 undifferentiated spondyloarthritis). One hundred and eighty-five healthy controls were also investigated. The presence of Cp infection was assessed in peripheral blood mononuclear cells using several PCR protocols targeting different regions of the Cp genome (16S-23S spacer rRNA, OMP-A, and Gro-EL). The DNA of other Chlamydia species (C. Pneumoniae and C. Trachomatis) was also investigated. Amplicons were sequenced to confirm the specificity of PCR products.
The presence of a subclinical chronic Cp infection was observed in a significantly higher percentage of patients with chronic polyarthritis (38/293; 13%) compared to healthy controls (1/185, 0.5%; OR=27.4, 95%CI:3.73-201.6, p<0.0001). Furthermore, the prevalence of Cp was higher in seronegative polyarthritis (23/118; 19.5%) than in seropositive RA patients (15/175; 7.4%; OR=2.58, 95%CI: 1.28-5.19, p=0.0078). The highest prevalence of Cp infection was found in RF/anti-CCP double-negative RA patients (13/46, 28.3%), followed by patients with psoriatic arthritis (6/36; 16.7%). No differences in age, sex, disease duration and undergoing therapies were noticed between Cp-positive and Cp-negative patients; nor between seropositive and seronegative patients.
Cp may be an infectious trigger possibly involved in the pathogenesis of a fraction of inflammatory polyarthritis, particularly in seronegative patients.
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