To identify the questing tick populations in urban and suburban areas from the city of Lugo (NW Spain), ticks were collected monthly by flagging. The presence of Borrelia spp., Rickettsia spp. and ...Anaplasma phagocytophilum also was determined by polymerase chain reaction (PCR) and sequence analysis. Overall, 342 questing ticks were collected; the tick abundance was higher in suburban (95.9%) than in urban areas (4.1%). Ixodes frontalis was the most abundant (86.5%); 88.5% were larvae, 11.1% nymphs and 0.3% adults. All development stages of I. ricinus (7.3%) and adults of Rhipicephalus sanguineus sensu lato (5.8%) and Dermacentor reticulatus (0.3%) were found. Rickettsia spp. (31.9%) was more prevalent than Borrelia spp. (2.7%); no ticks were positive to A. phagocytophilum. Six Rickettsia species were identified (R. slovaca, R. monacensis, R. massiliae, R. raoultii, R. sibirica subsp. mongolitimonae and R. aeschielmanii); Candidatus Rickettsia rioja and two novel Rickettsia species also were detected. In addition, Borrelia turdi (1.8%) and B. valaisiana (0.9%) were identified in Ixodes ticks. This is the first report of R. slovaca in R. sanguineus s.l. and of R. monacensis, R. raoultii, R. slovaca, R. sibirica subsp. mongolitimonae and Ca. R. rioja in I. frontalis. Since most of the pathogens detected are zoonotic, their presence in these areas may have implications for public health.
A higher number of ticks was collected in suburban than in urban areas, being Ixodes frontalis the most commonly detected species.
The results have significant implications for public health since most Rickettsia spp. detected are zoonotic.
This is the first report of the detection of Borrelia spp. and Rickettsia spp. DNA some species in questing I. frontalis.
A mathematical model was evaluated to predict the essential oil recovery by steam distillation of rosemary (
Rosmarinus officinalis L.), basil (
Ocimum basilicum L.), and lavender (
Lavandula dentata ...L.). The aim of the work was to obtain yield experimental data to compare with a mathematical model used in the simulation of essential oils extraction by steam distillation. The extraction model is based on single plate particle description on mass transfer. This model requires only one adjustable parameter, effective diffusion coefficient (
D), to predict the essential oil yield experimental curves.
Many teaching institutions worldwide are working on distance learning applications. In this field, remote laboratories are enabling intensive use of university facilities, while aiding the work of ...professors and students. The present paper introduces a platform designed to be used in industrial automation practical work. The platform is communicable through the Internet, includes programmable logic controllers that can be programmed with the open software CoDeSys, and has various sensors and actuators used in industrial installations. The various experimental stations of the platform, and the proposed laboratory exercises are described. The results of having used the platform with students are discussed.
BackgroundHigh alert medications (HAM) are drugs that bear a heightened risk of causing significant patient harm when used in error. The Institute for Safe Medication Practices (ISMP) created and ...periodically updates a list of potential HAM for acute care settings. The Joint Commission requires that hospitals have their own list of HAM and a process in place for managing those medications.PurposeTo evaluate the degree of knowledge of HAM among health professionals at a university hospital.Material and methodsThis was a descriptive cross sectional study in a university hospital with 1200 beds. The survey remained open for a period of 2 months between February and March 2016. The survey was developed by consensus with a panel of 5 hospital pharmacists and the quality coordinator. The survey was disseminated to prescribers, pharmacy and nursing staff throughout the hospital via email communication in order to assess differences across professionals. The survey was anonymous and voluntary. Responders were unpaid. The survey consisted of 4 questions with 4 response options and 1 correct answer. The first question assessed the knowledge of the definition of HAM. The second and third questions were about differences between HAM and others medications. The fourth question was knowledge of the list of HAM in poster format, developed by the pharmacy department of the hospital, placed in all hospital units. There was also a section for suggestions. The estimated response time was 5 min. Descriptive statistics were used to report the results of the survey.ResultsThe survey was sent to a total of 771 health professionals. 131 responses (17%) were received. Among the professionals who responded, 60% were physicians, 25% nurses and 15% pharmacists. 81.5% of survey participants were able to correctly define a HAM, 50% did not identify the HAM (70.3% were physicians, 23.5% nurses and 6.2% pharmacist). 74% of survey participants were unaware of the existence of a HAM poster in their hospital unit. For the suggestions section, 70% called for training sessions regarding HAM.ConclusionBased on the results obtained from the survey, we found that there was a good degree of knowledge deficiency about HAM. Further education is needed regarding HAM to guarantee patient safety.References and/or acknowledgementsISMP.No conflict of interest
▶ GC×GC-TOF-MS separated and characterised the composition of B. sarmientoi essential oil. ▶ Essential oil and pure guaiol, bulnesol and hanamyol proved to be effective antifungal agents with ...antifeedant effect. ▶ Neither B. sarmientoi oil nor the tested oil components showed phytotoxic activity.
Bulnesia sarmientoi Lorentz ex Griseb volatile oil was characterized by GC×GC-TOF-MS analysis. Major components were guaiol and bulnesol, followed by hanamyol. The enhanced sensitivity and superior resolution of GC×GC resulted in the identification of thus-far unreported oil constituents as β-guaiene, guaioxide, elemol, germacrene-B, eudesm-5-en-11-ol, γ-eudesmol, α-eudesmol and (−)-hanamyol. The insecticidal effect of B. sarmientoi oil and its main constituents (guaiol, bulnesol and hanamyol) on Spodoptera littoralis, Rhopalosiphum padi and Myzus persicae was studied. Guaiol affected the aphids in a dose-response fashion, showing low efficiency, while bulnesol and hanamyol were inactive. Both the oil and its constituents were also assayed for antifungal action against Fusarium spp. and phytotoxicity to Lactuca sativa. Among the pure compounds tested, bulnesol had a low-moderate effect on Fusarium moniliforme while hanamyol had a strong effect on Fusarium solani. Neither the oil nor the tested compounds affected L. sativa germination or radicle length, indicating that B. sarmientoi is not phytotoxic.
Preservation of blood vessel integrity, which is critical for normal physiology and organ function, is controlled at multiple levels, including endothelial junctions. However, the mechanism that ...controls the adequate assembly of endothelial cell junctions is not fully defined. Here, we uncover TAp73 transcription factor as a vascular architect that orchestrates transcriptional programs involved in cell junction establishment and developmental blood vessel morphogenesis and identify Angiomotin (AMOT) as a TAp73 direct transcriptional target. Knockdown of p73 in endothelial cells not only results in decreased Angiomotin expression and localization at intercellular junctions, but also affects its downstream function regarding Yes-associated protein (YAP) cytoplasmic sequestration upon cell–cell contact. Analysis of adherens junctional morphology after p73-knockdown in human endothelial cells revealed striking alterations, particularly a sharp increase in serrated junctions and actin bundles appearing as stress fibers, both features associated with enhanced barrier permeability. In turn, stabilization of Angiomotin levels rescued those junctional defects, confirming that TAp73 controls endothelial junction dynamics, at least in part, through the regulation of Angiomotin. The observed defects in monolayer integrity were linked to hyperpermeability and reduced transendothelial electric resistance. Moreover, p73-knockout retinas showed a defective sprout morphology coupled with hemorrhages, highlighting the physiological relevance of p73 regulation in the maintenance of vessel integrity in vivo
.
We propose a new model in which TAp73 acts as a vascular architect integrating transcriptional programs that will impinge with Angiomotin/YAP signaling to maintain junctional dynamics and integrity, while balancing endothelial cell rearrangements in angiogenic vessels.
A high-granularity timing detector for the ATLAS phase-II upgrade Casado, M.P.; Adam Bourdarios, C.; Belfkir, M. ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
2022, Letnik:
1032
Journal Article
Recenzirano
Odprti dostop
The large increase of pileup interactions is one of the main experimental challenges for the HL-LHC physics programme. A powerful new way to mitigate the effects of pileup is to use high-precision ...timing information to distinguish between collisions occurring close in space but well-separated in time. A High-Granularity Timing Detector, based on low gain avalanche detector technology, is therefore proposed for the ATLAS Phase-II upgrade. Covering the pseudorapidity region between 2.4 and 4.0, this device will improve the detector physics performance in the forward region. The typical number of hits per track in the detector was optimized so that the target average time resolution per track for a minimum-ionising particle is 30 ps at the start of lifetime, increasing to 50 ps at the end of HL-LHC operation. The high-precision timing information improves the pileup reduction to improve the forward object reconstruction, complementing the capabilities of the upgraded Inner Tracker (ITk) in the forward regions of ATLAS and leading to an improved performance for both jet and lepton reconstruction. These improvements in object reconstruction performance translate into sensitivity gains and enhance the reach of the ATLAS physics programme at the HL-LHC. In addition, the HGTD offers unique capabilities for the online and offline luminosity determination, an important requirement for precision physics measurements.
Myoblast C2C12 cells cultured in the presence of FGF2 actively proliferate and showed a differentiation-defective phenotype compared with cells cultured in low serum or in the presence of insulin. ...These FGF2 effects are associated with sustained activation of p44/p42-MAPK and lack of activation of AKT. Here we demonstrate that Sprouty-2, a protein involved in the negative feedback of receptor tyrosine kinase signaling, when stably overexpressed in C2C12 cells and in the presence of FGF2 produces growth arrest (precluding the expression of PCNA and the phosphorylation of retinoblastoma and inducing the expression of p21(CIP)) and myogenesis (multinucleated myotubes formation, induction of creatine kinase and expression of myosin heavy chain protein). These events were accompanied by repression of p44/p42-MAPK and activation of AKT. When C2C12 cells were stably transfected with a Sprouty-2 (Y55F) mutant defective in inhibiting p44/p42-MAPK activation by FGF, myoblasts in the presence of FGF continue to grow and completely fail to form myotubes. This work is the first evidence of the contribution of sprouty genes to myogenic differentiation in the presence of FGF2.
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