Patients with retinal diseases may exhibit serious complications that cause severe visual impairment owing to a lack of awareness of retinal diseases and limited medical resources. Understanding how ...artificial intelligence (AI) is used to make predictions and perform relevant analyses is a very active area of research on retinal diseases. In this study, the relevant Science Citation Index (SCI) literature on the AI of retinal diseases published from 2012 to 2021 was integrated and analyzed.
The aim of this study was to gain insights into the overall application of AI technology to the research of retinal diseases from set time and space dimensions.
Citation data downloaded from the Web of Science Core Collection database for AI in retinal disease publications from January 1, 2012, to December 31, 2021, were considered for this analysis. Information retrieval was analyzed using the online analysis platforms of literature metrology: Bibliometrc, CiteSpace V, and VOSviewer.
A total of 197 institutions from 86 countries contributed to relevant publications; China had the largest number and researchers from University College London had the highest H-index. The reference clusters of SCI papers were clustered into 12 categories. "Deep learning" was the cluster with the widest range of cocited references. The burst keywords represented the research frontiers in 2018-2021, which were "eye disease" and "enhancement."
This study provides a systematic analysis method on the literature regarding AI in retinal diseases. Bibliometric analysis enabled obtaining results that were objective and comprehensive. In the future, high-quality retinal image-forming AI technology with strong stability and clinical applicability will continue to be encouraged.
Background Exosomes as the main therapeutic vectors of mesenchymal stem cells (MSC) for inflammatory bowel disease (IBD) treatment and its mechanism remain unexplored. Tumor necrosis factor-alpha ...stimulated gene 6 (TSG-6) is a glycoprotein secreted by MSC with the capacities of tissue repair and immune regulation. This study aimed to explore whether TSG-6 is a potential molecular target of exosomes derived from MSCs (MSCs-Exo) exerting its therapeutic effect against colon inflammation and repairing mucosal tissue. Methods Two separate dextran sulfate sodium (DSS) and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced IBD mouse models were intraperitoneally administered MSCs-Exo extracted from human umbilical cord MSC (hUC-MSC) culture supernatant. Effects of MSCs-Exo on intestinal inflammation, colon barrier function, and proportion of T cells were investigated. We explored the effects of MSCs-Exo on the intestinal barrier and immune response with TSG-6 knockdown. Moreover, recombinant human TSG-6 (rhTSG-6) was administered exogenously and colon inflammation severity in mice was evaluated. Results Intraperitoneal injection of MSCs-Exo significantly ameliorated IBD symptoms and reduced mortality rate. The protective effect of MSCs-Exo on intestinal barrier was demonstrated evidenced by the loss of goblet cells and intestinal mucosa permeability, thereby improving the destruction of tight junctions (TJ) structures and microvilli, as well as increasing the expression of TJ proteins. Microarray analysis revealed that MSCs-Exo administration downregulated the level of pro-inflammatory cytokines and upregulated the anti-inflammatory cytokine in colon tissue. MSCs-Exo also modulated the response of Th2 and Th17 cells in the mesenteric lymph nodes (MLN). Reversely, knockdown of TSG-6 abrogated the therapeutic effect of MSCs-Exo on mucosal barrier maintenance and immune regulation, whereas rhTSG-6 administration showed similar efficacy to that of MSCs-Exo. Conclusions Our findings suggested that MSCs-Exo protected against IBD through restoring mucosal barrier repair and intestinal immune homeostasis via TSG-6 in mice. Keywords: Inflammatory bowel disease, Mesenchymal stem cells, Exosome, Tumor necrosis factor-alpha-stimulated gene 6, Intestinal barrier
The previous studies of human gametogenesis and embryogenesis have left many unanswered questions, which hinders the understanding of the physiology of these two vital processes and the development ...of diagnosis and treatment strategies for related diseases. Although many results have been obtained from animal studies, particularly mouse research, the results cannot be fully applied to humans due to species differences in physiology and pathology. However, due to ethical and material limitations, the direct study of human gametes and embryos is very difficult. The emergence and rapid development of organoids allow the construction of organoid systems that simulate gametogenesis and embryogenesis
, and many studies have successfully established organoid systems for some parts of or even the entire processes of gametogenesis and embryogenesis. These studies typically start with the establishment of mouse models and then modify these models to obtain human organoid models. These organoid models can be used to obtain a better understanding of the signaling pathways, molecular mechanisms, genetics, and epigenetic changes involved in gametogenesis and embryogenesis and could also be applied to clinical applications, such as drug screening. Here, we discuss the formation of primordial stem cell-like cells (PGCLCs), and in vitro-induced gametes and embryoids using pluripotent stem cells (PSCs). We also analyze their applications and limitations.
Exosomes (EXO) are extracellular vesicles with lipid bilayer membrane structure containing noncoding RNA, DNA, and other molecules which mediate biological functions. The importance of EXO derived ...from mesenchymal stem cells (MSCs) has been underlined in cardiovascular diseases. However, the functional role of long non-coding RNA (lncRNA) released by MSCs-EXO on atherosclerosis (AS) was unknown. We aimed to investigate the effects of lncRNA fetal-lethal non-coding developmental regulatory RNA (FENDRR) released from MSC-derived EXO on AS. The accumulation of oxidized low-density lipoprotein (oxLDL) caused AS in mice and damage to human vascular endothelial cells (HUV-EC-C). MSC-EXO restored HUV-EC-C activity and alleviated arterial injury. LncRNA microarrays revealed that FENDRR was delivered to cells and tissues by MSC-EXO. FENDRR bound to microRNA (miR)-28 to regulate TEA domain transcription factor 1 (TEAD1) expression. Moreover, FENDRR knockdown exacerbated cell injury and arterial injury in mice. miR-28 inhibitor reversed the effects of FENDRR silencing and reduced atherosclerotic plaque formation. While loss of TEAD1 mitigated the effect of miR-28 inhibitor and accentuated HUV-EC-C injury in vitro and AS symptoms in vivo. Our results demonstrated that MSC-EXO secreted FENDRR to treat AS. FENDRR competed with TEAD1 to bind to miR-28, thereby reducing HUV-EC-C injury and atherosclerotic plaque formation.
Nitric oxide (NO) has attracted significant attention as a stellar molecule. Presently, the study of NO has penetrated every field of life science, and NO is widely distributed in various tissues and ...organs. This review demonstrates the importance of NO in both male and female reproductive processes in numerous ways, such as in neuromodulation, follicular and oocyte maturation, ovulation, corpus luteum degeneration, fertilization, implantation, pregnancy maintenance, labor and menstrual cycle regulation, spermatogenesis, sperm maturation, and reproduction. However, the mechanism of action of some NO is still unknown, and understanding its mechanism may contribute to the clinical treatment of some reproductive diseases.
Polyurethane elastomer (PUE), which is widely used in coatings for construction, transportation, electronics, aerospace, and other fields, has excellent physical properties. However, polyurethane ...elastomers are flammable, which limits their daily use, so the flame retardancy of polyurethane elastomers is very important. Reactive flame retardants have the advantages of little influence on the physical properties of polymers and low tendency to migrate out. Due to the remarkable needs of non-halogenated flame retardants, phosphorus flame retardant has gradually stood out as the main alternative. In this review, we focus on the fire safety of PUE and provide a detailed overview of the current molecular design and mechanisms of reactive phosphorus-containing, as well as P-N synergistic, flame retardants in PUE. From the structural characteristics, several basic aspects of PUE are overviewed, including thermal performance, combustion performance, and mechanical properties. In addition, the perspectives on the future advancement of phosphorus-containing flame-retarded polyurethane elastomers (PUE) are also discussed. Based on the past research, this study provides prospects for the application of flame-retarded PUE in the fields of self-healing materials, bio-based materials, wearable electronic devices, and solid-state electrolytes.
In this paper, we study how to apply a periodic driving field to control stable spin tunneling in a non-Hermitian spin-orbit (SO) coupled bosonic double-well system. By means of a high-frequency ...approximation, we obtain the analytical Floquet solutions and their associated quasienergies and thus construct the general non-Floquet solutions of the dissipative SO coupled bosonic system. Based on detailed analysis of the Floquet quasienergy spectrum, the profound effect of system parameters and the periodic driving field on the stability of spin-dependent tunneling is investigated analytically and numerically for both balanced and unbalanced gain-loss between two wells. Under balanced gain and loss, we find that the stable spin-flipping tunneling is preferentially suppressed with the increase of gain-loss strength. When the ratio of Zeeman field strength to periodic driving frequency Ω/ω is even, there is a possibility that continuous stable parameter regions will exist. When Ω/ω is odd, nevertheless, only discrete stable parameter regions are found. Under unbalanced gain and loss, whether Ω/ω is even or odd, we can get parametric equilibrium conditions for the existence of stable spin tunneling. The results could be useful for the experiments of controlling stable spin transportation in a non-Hermitian SO coupled system.
The application of ethephon was investigated to examine its effects on both ethephon residue and quality properties of chili peppers during pre-harvest ripening with the goal of facilitating maximum ...commercial harvest along with improving color and flavor. A single ethephon treatment significantly increased
L
* and
a
* values and capsanthin concentration, while decreased total chlorophyll contents. Moreover, ethephon treatment induced significant promotion of capsaicin synthesis and reduction of soluble sugar content. While repeated treatments did not increase the total capsaicin content, and the consumption of soluble sugar was accelerated. Additionally, the maximum ethephon residue in chili pepper after ethephon treatment was 21.18 mg kg
−1
, which is lower than the permissible residue level of 50 mg kg
−1
for chili peppers. The ethephon residual decreased with prolonging harvest time of chili peppers. The effects of ethephon treatment on different types of chili peppers were variable. The results of this study indicated that ethephon could hasten the ripening process and increase the quality of chili peppers.
A smartphone-based detection platform for the determination of alkaline phosphatase (ALP) is described. The method is based on the rational design of the stimulus–response of ...7-methoxycoumarin-3-carboxylic acid (7-MC-3-COOH)–functionalized Eu-AMP infinite coordination polymer (ICP) nanoparticles. The blue fluorescence of 7-MC-3-COOH at 403 nm was suppressed, while the red fluorescence of Eu
3+
at 615 nm was sensitized after the formation of 7-MC-3-COOH@Eu-AMP ICP. Upon exposure to ALP, the dephosphorylation of AMP resulted in the destruction of 7-MC-3-COOH@Eu-AMP ICP, and thereby, the blue fluorescence of 7-MC-3-COOH recovered; in the meantime, the sensitized red fluorescence was quenched. With the fluorescence intensity ratio
F
615
/
F
430
as the signal readout, ALP can be detected within a concentration range 0.001 to 0.15 U mL
−1
, and the limit of detection (LOD) was 0.00035 U mL
−1
. Moreover, fluorescence color changes from red to blue could also be recognized by a portable device with the smartphone as a signal reader, and direct point-of-use testing (POUT) for ALP within a concentration range 0.005 to 0.7 U mL
−1
could be realized, with LOD of 0.0015 U mL
−1
. Endowed with high sensitivity and superior reliability, the assay enabled direct monitoring of P-related water eutrophication in a freshwater lake with ALP as an indicator.
Graphical abstract
A smartphone-based platform for point-of-use determination of alkaline phosphatase.
This study provides a new record of nitrogen (N) deposition and ecological response at a remote, high-elevation lake within the ecologically fragile Three River Headwaters Region, northwest China, ...from the early 20
th
Century to the past decade. A multi-proxy investigation of a lake sediment core including analyses of chironomids (non-biting midge), lake sediment geochemistry (δ
15
N, δ
13
C, TN, TOC, OC:TN), pollen, non-pollen palynomorphs and
210
Pb-dating. Results were compared with regional pollution forcings, including fertiliser application, atmospheric nitrate and dust deposition, and a localised multiscale air quality modelling system for N-deposition. Our data reflect a mesotrophic lake for the majority of the 20
th
Century with minimal anthropogenic impact. A shift in the lake conditions is evident after 1985 with increased nutrient enrichment. Low δ
15
N, OC:TN and increasing TN values, together with an increase in chironomid taxa associated with eutrophic conditions and macrophytes are exhibited in the record, particularly since 2002. These changes are consistent with nutrient loading causing a change in trophic status. Redundancy analysis (RDA) and partial RDAs verified TN as an important driver behind the shift in chironomid community composition in recent decades, explaining 22.2% of the variance on its own and 16.9% with other environmental variables partialled out. While temperature change since 1960 was not a primary control on the chironomid community of Lake Bander, a step-change increase in summer temperatures since the 1990s coincided with the disappearance of cold stenotherms from the record and seems to have exacerbated the shift to more productive conditions. This study highlights the reach of airborne N from modern agricultural and industrial activity to remote locations, and demonstrates the utility of palaeolimnological techniques as part of modern ecosystem assessment for conservation.
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