AIM: Climate change and other anthropogenic global change drivers act in complex, mutually exacerbating ways to alter the abundance and distribution of species. In South Africa, pied crows Corvus ...albus have increased in numbers and range in recent decades. Popular opinion links these changes to urbanisation and infrastructure development, but there has been no empirical test of this idea. We aimed to clarify the drivers of pied crow population changes in South Africa. LOCATION: South Africa. METHODS: We used publicly available long‐term datasets, the Southern African Bird Atlas Project and University of Delaware Gridded Climate Database, and spatial data from government bodies, to assess relationships between pied crow population and range changes, land use, infrastructure, urbanisation and climate change. RESULTS: Pied crow numbers have increased significantly in the past three decades, but rate of increase varied geographically, with crows declining in the northeast and increasing in the south‐west of South Africa. Pied crow population changes were strongly correlated with climate change. Crows have benefited most from climate warming in the shrubland biomes of south‐western South Africa. Pied crows are tree nesters, and within these shrublands, there is a strong positive relationship between the rate of population increase and the density of powerline infrastructure, which may facilitate pied crows’ increase by providing nesting sites. MAIN CONCLUSIONS: Pied crow numbers have increased in response to climate warming, with their spread facilitated by electrical infrastructure in south‐western South Africa, providing a clear example of compound influence of multiple global change drivers promoting a significant change in species range and reporting rate. Pied crows are generalist predators and there is popular concern about their ecological impact in areas where increases have occurred. We highlight the importance of understanding the ecosystem‐level implications of increased numbers of pied crows in South Africa's shrubland biomes.
We present clumps of dust emission from Herschel observations of the Large Magellanic Cloud (LMC) and their physical and statistical properties. We catalog cloud features seen in the dust emission ...from Herschel observations of the LMC, the Magellanic type irregular galaxy closest to the Milky Way, and compare these features with HI catalogs from the ATCA+Parkes HI survey. Using an automated cloud-finding algorithm, we identify clouds and clumps of dust emission and examine the cumulative mass distribution of the detected dust clouds. The mass of cold dust is determined from physical parameters that we derive by performing spectral energy distribution fits to 250, 350, and 500 micronm emission from SPIRE observations using DUSTY and GRASIL radiative transfer calculation with dust grain size distributions for graphite/silicate in low-metallicity extragalactic environments. The dust cloud mass spectrum follows a power law distribution with an exponent of gamma=-1.8 for clumps larger than 400 solar mass and is similar to the HI mass distribution. This is expected from the theory of ISM structure in the vicinity of star formation.
Membranous nephropathy results from subepithelial antigen-antibody complex deposition along the glomerular basement membrane. Although PLA2R, THSD7A, and NELL-1 account for a majority (about 80%) of ...the target antigens, the target antigen in the remaining cases is not known. Using laser microdissection of PLA2R-negative glomeruli of patients with membranous nephropathy followed by mass spectrometry we identified a unique protein, Semaphorin 3B, in three cases. Mass spectrometry failed to detect Semaphorin-3B in 23 PLA2R-associated cases of membranous nephropathy and 88 controls. Semaphorin 3B in all three cases was localized to granular deposits along the glomerular basement membrane by immunohistochemistry. Next, an additional eight cases of Semaphorin 3B-associated membranous nephropathy were identified in three validation cohorts by immunofluorescence microscopy. In four of 11 cases, kidney biopsy also showed tubular basement membrane deposits of IgG on frozen sections. Confocal microscopy showed that both IgG and Semaphorin 3B co-localized to the glomerular basement membrane. Western blot analysis of five available sera showed reactivity to reduced Semaphorin 3B in four of four patients with active disease and no reactivity in one patient in clinical remission; there was also no reactivity in control sera. Eight of the 11 cases of Semaphorin 3B-associated membranous nephropathy were pediatric cases. Furthermore, in five cases, the disease started at or below the age of two. Thus, Semaphorin 3B-associated membranous nephropathy appears to be a distinct type of disease; more likely to be present in pediatric patients.
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We report observations of the 2(12)-1(01) rotational transition of the 13C isotopic species of cyclopropenylidene (C3H2) toward TMC-1, Sgr B2, and IRC +10216 using the laboratory rest frequencies ...which have recently become available. Our detections allow estimates to be made of the fractional abundance of the unsubstituted similar species in these sources. The fractional abundance relative to H2, f(C3H2), is 1-2 x 10(-8) in TMC-1, and this is similar to the abundance of HCN, one of the more abundant organic molecules in the interstellar medium. In IRC +10216 f(C3H2) is one order of magnitude greater than in TMC-1. The 12C species in Sgr B2 shows a self-absorbed profile and the relative abundance of C3H2 estimated to be about an order of magnitude less than in TMC-1.
•PBK models have helped to facilitate quantitative in vitro to in vivo extrapolation.•PBK modelling has the potential to play a significant role in reducing animal testing.•It is critical to assess ...the validity of PBK models built using non-animal data.•A framework is needed for communicating characteristics and results of PBK modelling.
The fields of toxicology and chemical risk assessment seek to reduce, and eventually replace, the use of animals for the prediction of toxicity in humans. In this context, physiologically based kinetic (PBK) modelling based on in vitro and in silico kinetic data has the potential to a play significant role in reducing animal testing, by providing a methodology capable of incorporating in vitro human data to facilitate the development of in vitro to in vivo extrapolation of hazard information. In the present article, we discuss the challenges in: 1) applying PBK modelling to support regulatory decision making under the toxicology and risk-assessment paradigm shift towards animal replacement; 2) constructing PBK models without in vivo animal kinetic data, while relying solely on in vitro or in silico methods for model parameterization; and 3) assessing the validity and credibility of PBK models built largely using non-animal data. The strengths, uncertainties, and limitations of PBK models developed using in vitro or in silico data are discussed in an effort to establish a higher degree of confidence in the application of such models in a regulatory context. The article summarises the outcome of an expert workshop hosted by the European Commission Joint Research Centre (EC-JRC) – European Union Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM), on “Physiologically-Based Kinetic modelling in risk assessment – reaching a whole new level in regulatory decision-making” held in Ispra, Italy, in November 2016, along with results from an international survey conducted in 2017 and recently reported activities occurring within the PBK modelling field. The discussions presented herein highlight the potential applications of next generation (NG)-PBK modelling, based on new data streams.
Memory T cells are thought to rely on oxidative phosphorylation and short-lived effector T cells on glycolysis. Here, we investigated how T cells arrive at these states during an immune response. To ...understand the metabolic state of rare, early-activated T cells, we adapted mass cytometry to quantify metabolic regulators at single-cell resolution in parallel with cell signaling, proliferation, and effector function. We interrogated CD8+ T cell activation in vitro and in response to Listeria monocytogenes infection in vivo. This approach revealed a distinct metabolic state in early-activated T cells characterized by maximal expression of glycolytic and oxidative metabolic proteins. Cells in this transient state were most abundant 5 days post-infection before rapidly decreasing metabolic protein expression. Analogous findings were observed in chimeric antigen receptor (CAR) T cells interrogated longitudinally in advanced lymphoma patients. Our study demonstrates the utility of single-cell metabolic analysis by mass cytometry to identify metabolic adaptations of immune cell populations in vivo and provides a resource for investigations of metabolic regulation of immune responses across a variety of applications.
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•A mass cytometry approach quantifies metabolic proteins in single cells in vivo•Early-activated T cells exhibit simultaneous peak oxidative and glycolytic activity•CD8+ T cells transit through this transient state prior to differentiation•CAR T cells exhibit an analogous transient program upon infusion into patients
Levine, Hiam-Galvez, et al. develop a mass-cytometry-based approach to quantify metabolic protein expression in single cells in vivo, revealing a distinct metabolic state early after CD8+ T cell activation characterized by simultaneous expression of glycolytic and oxidative proteins. This approach provides a resource for the study of metabolic regulation across a variety of applications.
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