Rotating Rayleigh–Bénard convection is investigated numerically with the use of an asymptotic model that captures the rapidly rotating, small Ekman number limit, $Ek \rightarrow 0$. The Prandtl ...number ($Pr$) and the asymptotically scaled Rayleigh number ($\widetilde {Ra} = Ra Ek^{4/3}$, where $Ra$ is the typical Rayleigh number) are varied systematically. For sufficiently vigorous convection, an inverse kinetic energy cascade leads to the formation of a pair of large-scale vortices of opposite polarity, in agreement with previous studies of rapidly rotating convection. With respect to the kinetic energy, we find a transition from convection dominated states to a state dominated by large-scale vortices at an asymptotically reduced (small-scale) Reynolds number of $\widetilde {Re} \approx 6$ ($\widetilde {Re} = Re Ek^{1/3}$, where $Re$ is the Reynolds number associated with vertical flows) for all investigated values of $Pr$. The ratio of the depth-averaged kinetic energy to the kinetic energy of the convection reaches a maximum at $\widetilde {Re} \approx 24$, then decreases as $\widetilde {Ra}$ is increased. This decrease in the relative kinetic energy of the large-scale vortices is associated with a decrease in the convective correlations with increasing Rayleigh number. The scaling behaviour of the convective flow speeds is studied; although a linear scaling of the form $\widetilde {Re} \sim \widetilde {Ra}/Pr$ is observed over a limited range in Rayleigh number and Prandtl number, a clear departure from this scaling is observed at the highest accessible values of $\widetilde {Ra}$. Calculation of the forces present in the governing equations shows that the ratio of the viscous force to the buoyancy force is an increasing function of $\widetilde {Ra}$, that approaches unity over the investigated range of parameters.
Somatic Symptom Disorder Kurlansik, Stuart L; Maffei, Mario S
American family physician,
2016-Jan-01, Letnik:
93, Številka:
1
Journal Article
Recenzirano
With the release of the Diagnostic and Statistical Manual of Mental Disorders, 5th ed., the diagnostic category previously known as somatoform disorders is now called somatic symptom and related ...disorders. The revisions were intended to increase their relevance in the primary care setting. The main feature of this disorder is a patient's concern with physical symptoms that he or she attributes to a nonpsychiatric disease. Primary care physicians often treat patients who manifest symptoms for which there are no biologic cause, and patients with somatic symptom disorder may be subjected to unnecessary testing and procedures. As a result, appropriate diagnosis is essential. Screening instruments are useful in determining the presence of somatic symptom disorder. It is important for the primary care physician to schedule regular appointments, establish a strong therapeutic alliance, acknowledge and legitimize the patient's symptoms, and limit diagnostic testing or referrals to subspecialists. Proven treatments include cognitive behavior therapy, mindfulness-based therapy, and pharmacotherapy. The use of selective serotonin reuptake inhibitors or tricyclic antidepressants has been effective in alleviating symptoms. Referral to a mental health professional may be necessary when treatment by the primary care physician is ineffective.
Menopausal disorders may include shorter-term symptoms, such as hot flushes and night sweats (vasomotor symptoms, VMS) and longer-term chronic conditions such as cardiovascular disease (CVD), ...osteoporosis, and cognitive impairment. Initially, no clear link between the shorter-term symptoms and longer-term chronic conditions was evident and these disorders seemed to occur independently from each other. However, there is a growing body of evidence demonstrating that VMS may be a biomarker for chronic disease. In this review, the association between VMS and a range of chronic postmenopausal conditions including CVD, osteoporosis, and cognitive decline is discussed. Prevention of CVD in women, as for men, should be started early, and effective management of chronic disease in postmenopausal women has to start with the awareness that VMS during menopause are harbingers of things to come and should be treated accordingly.
Most investigations into cancer cell drug response are performed with cells cultured on flat (2D) tissue culture plastic. Emerging research has shown that the presence of a three-dimensional (3D) ...extracellular matrix (ECM) is critical for normal cell behavior including migration, adhesion, signaling, proliferation and apoptosis. In this study we investigate differences between cancer cell signaling in 2D culture and a 3D ECM, employing real-time, live cell tracking to directly observe U2OS human osteosarcoma and MCF7 human breast cancer cells embedded in type 1 collagen gels. The activation of the important PI3K signaling pathway under these different growth conditions is studied, and the response to inhibition of both PI3K and mTOR with PI103 investigated. Cells grown in 3D gels show reduced proliferation and migration as well as reduced PI3K pathway activation when compared to cells grown in 2D. Our results quantitatively demonstrate that a collagen ECM can protect U2OS cells from PI103. Overall, our data suggests that 3D gels may provide a better medium for investigation of anti-cancer drugs than 2D monolayers, therefore allowing better understanding of cellular response and behavior in native like environments.
Extracellular matrix (ECM) remodeling is a key component of cell migration and tumor metastasis, and has been associated with cancer progression. Despite the importance of matrix remodeling, ...systematic and quantitative studies on the process have largely been lacking. Furthermore, it remains unclear if the disrupted tensional homeostasis characteristic of malignancy is due to initially altered ECM and tissue properties, or to the alteration of the tissue by tumor cells. To explore these questions, we studied matrix remodeling by two different prostate cancer cell lines in a three-dimensional collagen system. Over one week, we monitored structural changes in gels of varying collagen content using confocal reflection microscopy and quantitative image analysis, tracking metrics of fibril fraction, pore size, and fiber length and diameter. Gels that were seeded with no cells (control), LNCaP cells, and DU-145 cells were quantitatively compared. Gels with higher collagen content initially had smaller pore sizes and higher fibril fractions, as expected. However, over time, LNCaP- and DU-145-populated matrices showed different structural properties compared both to each other and to the control gels, with LNCaP cells appearing to favor microenvironments with lower collagen fiber fractions and larger pores than DU-145 cells. We posit that the DU-145 cells' preference for denser matrices is due to their higher invasiveness and proteolytic capabilities. Inhibition of matrix proteases resulted in reduced fibril fractions for high concentration gels seeded with either cell type, supporting our hypothesis. Our novel quantitative results probe the dynamics of gel remodeling in three dimensions and suggest that prostate cancer cells remodel their ECM in a synergistic manner that is dependent on both initial matrix properties as well as their invasiveness.
The Surface Expression of Deep Columnar Flows Holdenried‐Chernoff, D.; Maffei, S.; Jackson, A.
Geochemistry, geophysics, geosystems,
June 2020, 2020-06-00, 20200601, 2020-06-01, Letnik:
21, Številka:
6
Journal Article
Recenzirano
Odprti dostop
Studies of the Earth's secular variation have blossomed in recent decades, due to the availability of brand‐new satellite data. In particular, flow inversions at the core surface that incorporate the ...effects of rapid rotation a priori have been able to provide new insight into flows with time scales as short as a few years. These models have been mostly developed in a cylindrical representation, while core inversion methods are based on a spherical surface representation of the flow. This technical brief links band‐limited expressions for the flow coefficients in a cylindrical representation and those in a spherical surface representation and demonstrates that these also remain band limited, with equal degrees of freedom. Correct regularity conditions on the stream function ensure a regular representation of these flows on the spherical surface. A triangular truncation rule for the cylindrical representation that is applicable for “isotropic resolution” is also presented. The equations we derive will be of use in future data‐assimilation work employing this cylindrical flow representation and may prove helpful for conventional core‐inversion strategies.
Key Points
We present an exact band‐limited relationship between a cylindrical flow representation and its spherical surface counterpart
Introduction
Delusions are a key feature of schizophrenia psychopathology. From a phenomenological approach, Jaspers (1913) differentiates between “primary” or true schizophrenic delusions, defined ...as an unmediated phenomenon that cannot be understood in terms of prior psychological origin or motivation, and “secondary” delusions, understandable from the patient’s mood state or personality. Primary delusions have been considered the hallmark of reality distorsion dimension in schizophrenia, disregarding a possible affective patwhay to delusional belief.
Objectives
The present study was aimed at elucidating the psychopathological trajectories to delusion in schizophrenia through the investigation of both affective and schizotypal trait dispositions.
Methods
Seventy-eight participants affected by schizophrenia were administered the Peters Delusional Inventory (PDI), the Positive and Negative Affective Scale (PANAS), the Experience of Shame Scale (ESS), the Referential Thinking Scale (REF), the Magical Ideation Scale (MIS) and the Perceptual Aberration Scale (PAS).
Results
The severity of delusional ideation (PDI) was positively related to both affective (PANAS positive dimension, ESS) and schizotypal traits (MIS, PAS and REF). Moreover, referential thinking (REF) mediated the relationship between “magical ideation” (MIS) and delusions severity (Fig. 1), whereas experience of shame (ESS) was a moderating factor in the between referential thinking and delusion severity (Fig. 2).
Conclusions
The study findings suggest that in schizophrenia patients, severity of delusions is underpinned by an intertwining of both affective and schizotypal pathways.
Disclosure
No significant relationships.
Purpose. Oral bisphosphonates (BPs) are the most commonly used medications for osteoporosis (OP), but their poor gastrointestinal (GI) absorption and tolerance hamper compliance. Intramuscular (IM) ...neridronate (NE), an amino-BP, is an easy-to-administer, effective, and safe alternative to oral BPs. We assessed the 6-year effects of monthly IM NE on bone mineral density (BMD) and bone turnover biomarkers (BMs) in postmenopausal OP. Methods. This single-center, prospective study enrolled postmenopausal osteoporotic outpatients with gastric intolerance to BPs (based on Tuscany Region’s law GRT n. 836 20/10/2008). They received 25 mg IM NE once a month (with vitamin D and calcium if necessary) for 6 years. BMD was evaluated at lumbar spine (L1-L4), femoral neck (FN), and total femur (TF) at baseline (BL) and every 12 months afterwards. At BL, month 3, and every 12 months after BL, total and ionized calcium, vitamin D, parathyroid hormone 1-84, bone alkaline phosphatase (BALP), osteocalcin, and N- and C-terminal telopeptides were assayed. Results. Overall, 60 women (mean age: 62.3±7.5 years) received monthly IM NE for 6 years, with vitamin D and calcium supplementation in 81.3% of cases. Compared to BL, BMD increased significantly already after 1 year at all sites (4.5±0.9% for L1-L4, 4.5±0.8% for TF, and 2.1±0.6% for FN, P≤0.05), and the changes were maintained over time, whereas FN further improved up to year 3 and remained stable afterwards (P≤0.05). All BMs, except for total calcium and BALP, progressively decreased over time (P≤0.05). No fractures and significant adverse events were reported. Conclusion. The monthly administration of IM NE represents a manageable and effective option, in terms of BMD and bone BM improvement, for the long-term treatment of postmenopausal OP women with gastric intolerance to BPs. This trial is registered with ClinicalTrials.gov Identifier: NCT03699150.
PDF
