We perform landslide susceptibility zonation with slope units using three digital elevation models (DEMs) of varying spatial resolution of the Ubaye Valley (South French Alps). In so doing, we ...applied a recently developed algorithm automating slope unit delineation, given a number of parameters, in order to optimize simultaneously the partitioning of the terrain and the performance of a logistic regression susceptibility model. The method allowed us to obtain optimal slope units for each available DEM spatial resolution. For each resolution, we studied the susceptibility model performance by analyzing in detail the relevance of the conditioning variables. The analysis is based on landslide morphology data, considering either the whole landslide or only the source area outline as inputs. The procedure allowed us to select the most useful information, in terms of DEM spatial resolution, thematic variables and landslide inventory, in order to obtain the most reliable slope unit-based landslide susceptibility assessment.
GIS-based deterministic models may be used for landslide susceptibility mapping over large areas. However, such efforts require specific strategies to (i) keep computing time at an acceptable level, ...and (ii) parameterize the geotechnical data. We test and optimize the performance of the GIS-based, 3-D slope stability model r.slope.stability in terms of computing time and model results. The model was developed as a C- and Python-based raster module of the open source software GRASS GIS and considers the 3-D geometry of the sliding surface. It calculates the factor of safety (FoS) and the probability of slope failure (Pf) for a number of randomly selected potential slip surfaces, ellipsoidal or truncated in shape. Model input consists of a digital elevation model (DEM), ranges of geotechnical parameter values derived from laboratory tests, and a range of possible soil depths estimated in the field. Probability density functions are exploited to assign Pf to each ellipsoid. The model calculates for each pixel multiple values of FoS and Pf corresponding to different sliding surfaces. The minimum value of FoS and the maximum value of Pf for each pixel give an estimate of the landslide susceptibility in the study area. Optionally, r.slope.stability is able to split the study area into a defined number of tiles, allowing parallel processing of the model on the given area. Focusing on shallow landslides, we show how multi-core processing makes it possible to reduce computing times by a factor larger than 20 in the study area. We further demonstrate how the number of random slip surfaces and the sampling of parameters influence the average value of Pf and the capacity of r.slope.stability to predict the observed patterns of shallow landslides in the 89.5 km2 Collazzone area in Umbria, central Italy.
Choloylglycine hydrolase (CGH), a bile salt hydrolase, has been annotated in all the available genomes of Brucella species. We obtained the Brucella CGH in recombinant form and demonstrated in vitro ...its capacity to cleave glycocholate into glycine and cholate. Brucella abortus 2308 (wild type) and its isogenic Δcgh deletion mutant exhibited similar growth rates in tryptic soy broth in the absence of bile. In contrast, the growth of the Δcgh mutant was notably impaired by both 5% and 10% bile. The bile resistance of the complemented mutant was similar to that of the wild-type strain. In mice infected through the intragastric or the intraperitoneal route, splenic infection was significantly lower at 10 and 20 days postinfection in animals infected with the Δcgh mutant than in those infected with the wild-type strain. For both routes, no differences in spleen CFU were found between animals infected with the wild-type strain and those infected with the complemented mutant. Mice immunized intragastrically with recombinant CGH mixed with cholera toxin (CGH+CT) developed a specific mucosal humoral (immunoglobulin G IgG and IgA) and cellular (interleukin-2) immune responses. Fifteen days after challenge by the same route with live B. abortus 2308 cells, splenic CFU counts were 10-fold lower in mice immunized with CGH+CT than in mice immunized with CT or phosphate-buffered saline. This study shows that CGH confers on Brucella the ability to resist the antimicrobial action of bile salts. The results also suggest that CGH may contribute to the ability of Brucella to infect the host through the oral route.
Systematic and timely documentation of triggered (i.e. event) landslides is fundamental to build extensive datasets worldwide that may help define and/or validate trends in response to climate ...change. More in general, preparation of landslide inventories is a crucial activity since it provides the basic data for any subsequent analysis. In this work we present an event landslide inventory map (E-LIM) that was prepared through a systematic reconnaissance field survey in about 1 month after an extreme rainfall event hit an area of about 5000 km
in the Marche-Umbria regions (central Italy). The inventory reports evidence of 1687 triggered landslides in an area of ~550 km
. All slope failures were classified according to type of movement and involved material, and documented with field pictures, wherever possible. The database of the inventory described in this paper as well as the collection of selected field pictures associated with each feature is publicly available at figshare.
We describe a semi-automatic procedure for the classification of satellite imagery into landslide or no landslide categories, aimed at preparing event landslide inventory maps. The two-steps ...procedure requires knowledge of the occurrence of a landslide event, availability of a pre- and post- event pseudo-stereo pair and a digital elevation model. The first step consists in the evaluation of a discriminant function, applied to a combination of well-known change detection indices tuned on landslide spectral response. The second step is devoted to discriminant function classification, aimed at distinguishing the only landslide class, through an improvement of the usual 'thresholding' method. We devised a multi-threshold classification, in which thresholding is applied separately in small subsets of the scene. We show that using slope units as topographic-aware subsets produces best classification performance when compared to the ground truth of a landslide inventory prepared by visual interpretation. The method proved to be superior to the use of a single threshold and to any multi-threshold procedure based on topography-blind subdivisions of the scene, especially in the validation stage. We argue that the improved classification performance and limited training requirements represent a step forward towards an automatic, real-time landslide mapping from satellite imagery.
Several bacterial pathogens have TIR domain-containing proteins that contribute to their pathogenesis. We identified a second TIR-containing protein in Brucella spp. that we have designated BtpB. We ...show it is a potent inhibitor of TLR signaling, probably via MyD88. BtpB is a novel Brucella effector that is translocated into host cells and interferes with activation of dendritic cells. In vivo mouse studies revealed that BtpB is contributing to virulence and control of local inflammatory responses with relevance in the establishment of chronic brucellosis. Together, our results show that BtpB is a novel Brucella effector that plays a major role in the modulation of host innate immune response during infection.
PDF
