Activity recognition systems are a large field of research and development, currently with a focus on advanced machine learning algorithms, innovations in the field of hardware architecture, and on ...decreasing the costs of monitoring while increasing safety. This article concentrates on the applications of activity recognition systems and surveys their state of the art. We categorize such applications into active and assisted living systems for smart homes, healthcare monitoring applications, monitoring and surveillance systems for indoor and outdoor activities, and tele-immersion applications. Within these categories, the applications are classified according to the methodology used for recognizing human behavior, namely, based on visual, non-visual, and multimodal sensor technology. We provide an overview of these applications and discuss the advantages and limitations of each approach. Additionally, we illustrate public data sets that are designed for the evaluation of such recognition systems. The article concludes with a comparison of the existing methodologies which, when applied to real-world scenarios, allow to formulate research questions for future approaches.
The hexosamine pathway (HP) is a key anabolic pathway whose product uridine 5'-diphospho-N-acetyl-D-glucosamine (UDP-GlcNAc) is an essential precursor for glycosylation processes in mammals. It ...modulates the ER stress response and HP activation extends lifespan in Caenorhabditis elegans. The highly conserved glutamine fructose-6-phosphate amidotransferase 1 (GFAT-1) is the rate-limiting HP enzyme. GFAT-1 activity is modulated by UDP-GlcNAc feedback inhibition and via phosphorylation by protein kinase A (PKA). Molecular consequences of GFAT-1 phosphorylation, however, remain poorly understood. Here, we identify the GFAT-1 R203H substitution that elevates UDP-GlcNAc levels in C. elegans. In human GFAT-1, the R203H substitution interferes with UDP-GlcNAc inhibition and with PKA-mediated Ser205 phosphorylation. Our data indicate that phosphorylation affects the interactions of the two GFAT-1 domains to control catalytic activity. Notably, Ser205 phosphorylation has two discernible effects: it lowers baseline GFAT-1 activity and abolishes UDP-GlcNAc feedback inhibition. PKA controls the HP by uncoupling the metabolic feedback loop of GFAT-1.
Chiral alcohols constitute important building blocks that can be produced enantioselectively by using nicotinamide adenine dinucleotide (phosphate) NAD(P)H‐dependent oxidoreductases. For NAD(P)H ...regeneration, electricity delivers the cheapest reduction equivalents. Enzymatic electrosynthesis suffers from cofactor and enzyme instability, whereas microbial electrosynthesis (MES) exploits whole cells. Here, we demonstrate MES by using resting Escherichia coli as biocatalytic chassis for a production platform towards fine chemicals through electric power. This chassis was exemplified for the synthesis of chiral alcohols by using a NADPH‐dependent alcohol dehydrogenase from Lactobacillus brevis for synthesis of (R)‐1‐phenylethanol from acetophenone. The E. coli strain and growth conditions affected the performance. Maximum yields of (39.4±5.7) % at a coulombic efficiency of (50.5±6.0) % with enantiomeric excess >99 % was demonstrated at a rate of (83.5±13.9) μm h−1, confirming the potential of MES for synthesis of high‐value compounds.
Chiral building blocks from electric power: By using resting Escherichia coli as biocatalytic chassis a production platform towards chiral alcohols is developed. This is exemplified for the enantioselective synthesis of (R)‐1‐phenylethanol from acetophenone.
SUMMARY
We present palaeo- and rock magnetic results from a well-dated, 21 m-thick, Late Pleistocene continental sedimentary section located in southern Germany. Rock magnetic measurements reveal a ...complex magnetic mineralogy dominated by low coercivity minerals likely related to single domain biogenic magnetite and biogenic or early diagenetic greigite. In the lower part of the section also detrital haematite is present. The stable remanence shows predominantly normal polarity with two marked deviations at ∼1280-1200 cm and at 886 cm profile depth. Whereas the lower excursion is well established by several samples and documented also by detrital haematite, the upper one is only represented by a single sample and revealed by magnetite and greigite. Using the radiocarbon-based age model for the section, the lower excursion yields an age of 42.8–41.3 ka cal BP and is interpreted to represent the Laschamps geomagnetic excursion. The increased abundance of greigite in the upper part of the section, especially in the sample responsible for the upper anomalous direction, renders the interpretation of an actual excursion problematic due to the reducing environment necessary for the greigite formation.
Particulate organic matter (POM) and dissolved organic carbon (DOC) release by six dominant hermatypic coral genera (Acropora, Fungia, Goniastrea, Millepora, Pocillopora and Stylophora) were measured ...under undisturbed conditions by laboratory incubations during four seasonal expeditions to the Northern Red Sea. In addition, the influence of environmental factors (water temperature, light availability and ambient inorganic nutrient concentrations) was evaluated. Particulate organic carbon (POC) and particulate nitrogen (PN) release were always detectable and genus-specific, with Stylophora releasing most POM (6.5 mg POC and 0.5 mg PN m⁻² coral surface area h⁻¹) during all seasons. The fire coral Millepora released significantly less POM (0.3 mg POC and 0.04 mg PN m⁻² coral surface area h⁻¹) than all investigated anthozoan genera. The average POC:PN ratio of POM released by all coral genera was 12 ± 1, indicating high carbon/low nitrogen content of coral-derived organic matter. POM release showed little seasonal variation, but average values of POC and PN release rates correlated with water temperature, light availability and ambient nitrate concentrations. DOC net release and elevated DOC:POC ratios were detectable for Acropora, Goniastrea and Millepora, revealing maximum values for Acropora (30.7 mg DOC m⁻² coral surface area h⁻¹), whilst predominant DOC uptake was observed for Pocillopora, Fungia and Stylophora. Depth-mediated light availability influenced DOC fluxes of Acropora and Fungia, while fluctuations in water temperature and ambient inorganic nutrient concentrations showed no correlation. These comprehensive data provide an important basis for the understanding of coral reef organic matter dynamics and relevant environmental factors.
The European Society of Breast Cancer Specialists (EUSOMA) has fostered a voluntary certification process for breast centres to establish minimum standards and ensure specialist multidisciplinary ...care. Prospectively collected anonymous information on primary breast cancer cases diagnosed and treated in the units is transferred annually to a central EUSOMA data warehouse for continuous monitoring of quality indicators (QIs) to improve quality of care. Units have to comply with the EUSOMA Breast Centre guidelines and are audited by peers. The database was started in 2006 and includes over 110,000 cancers from breast centres located in Germany, Switzerland, Belgium, Austria, The Netherlands, Spain, Portugal and Italy. The aim of the present study is assessing time trends of QIs in EUSOMA-certified breast centres over the decade 2006–2015.
Previously defined QIs were calculated for 22 EUSOMA-certified breast centres (46122 patients) during 2006–2015.
On the average of all units, the minimum standard of care was achieved in 8 of 13 main EUSOMA QIs in 2006 and in all in 2015. All QIs, except removal of at least 10 lymph nodes at axillary clearance and oestrogen receptor–negative tumours (T > 1 cm or N+) receiving adjuvant chemotherapy, improved significantly in this period. The desirable target was reached for two QIs in 2006 and for 7 of 13 QIs in 2015.
The EUSOMA model of audit and monitoring QIs functions well in different European health systems and results in better performance of QIs over the last decade. QIs should be evaluated and adapted on a regular basis, as guidelines change over time.
•The time trends of quality indicators in EUSOMA-certified breast centres over the decade 2006–2015 are evaluated.•The EUSOMA model of audit and monitoring QIs functions well in different European health systems.•Audit and measuring quality indicators result in better performance.
Summary Chronic hepatitis E has been described several times in strongly immunosuppressed HIV-patients. We describe the persistence of HEV-infection in an HIV-patient despite a restored immune ...response. This case demonstrates that HEV-infection can persist in formerly immunosuppressed individuals irrespective of the current immune status. Persisting HEV-infection can lead to chronic inflammation and liver cirrhosis. Physicians should be aware of the possibility of chronic hepatitis E even in patients that are not any longer immunocompromised. However, ribavirin is an efficient treatment option.
Multi‐isotope fingerprints in the bioapatite of archaeological skeletons are mostly superior over single isotope analyses for provenance studies. Gaussian mixture model (GMM) clustering is a novel ...tool for a similarity search among multidimensional data sets and at the same time permits the evaluation of the structural importance of particular isotopic ratios in the data set. We applied three GMM clustering experiments on multi‐isotope fingerprints—stable strontium (Sr), lead (Pb) and oxygen (O) isotopic ratios—established in 217 archaeological animal bones excavated along a specific transect across the European Alps. This reference region had been in use since prehistoric times by humans who crossed the Alps from north to south, and vice versa. The resulting clusters permit a spatial assignment of the specimens with a very high probability, in particular with regard to the geological complexity of the region. A combination of Sr with Pb stable isotopes led to an optimal differentiation between the southern and northern Alpine forelands that cannot be distinguished from each other by 87Sr/86Sr ratios alone, while the contribution of δ18O is not particularly high. The isotopic mapping and subsequent cluster analysis is suitable for the analysis of archaeological human finds and the reconstruction of the direction of transalpine mobility and trade.
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