The General Antiparticle Spectrometer (GAPS) is a balloon-borne experiment that aims to measure low-energy cosmic-ray antiparticles. GAPS has developed a new antiparticle identification technique ...based on exotic atom formation caused by incident particles, which is achieved by ten layers of Si(Li) detector tracker in GAPS. The conventional analysis uses the physical quantities of the reconstructed incident and secondary particles. In parallel with this, we have developed a complementary approach based on deep neural networks. This paper presents a new convolutional neural network (CNN) technique. A three-dimensional CNN takes energy depositions as three-dimensional inputs and learns to identify their positional/energy correlations. The combination of the physical quantities and the CNN technique is also investigated. The findings show that the new technique outperforms existing machine learning-based methods in particle identification.
The purpose of this study is to understand the structure of the issues related to use of ambulance services by the elderly，and to obtain insights for strategies for solving the issues. ...Semi-structured individual or focus-group interviews were conducted on 216 healthcare professionals from 31 institutions in Kobe，including 68 physicians，59 nurses，6 policy makers. Thematic analyses were performed. The major themes identified included： 1） unclear preferences of patients about future medical care，2） barriers to ACP implementation，3） unavailability of sharing health information about ACP preference and physical conditions of patients，4） lack of cooperation among emergency department，community medical services，and long-term care facilities，and 5） insufficient local resources and public involvement． The proposals for solving these issues included; 1） clarification of the definitions，components，and evaluation methods of ACP suitable for the Japanese ，2） establishment of electronic sharing system about medical information including ACP of patients ，3） reduction of unwanted or unnecessary emergency transportation and hospitalization through cooperation of emergency department，primary care services，and long-term care facilities，and 4） fostering culture and system to think together with the residents and work toward a better society.
The General Antiparticle Spectrometer (GAPS) is an upcoming balloon mission to measure low-energy cosmic-ray antinuclei during at least three ~35-day Antarctic flights. With its large geometric ...acceptance and novel exotic atom-based particle identification, GAPS will detect ~500 cosmic antiprotons per flight and produce a precision cosmic antiproton spectrum in the kinetic energy range of ~0.07-0.21 GeV/n at the top of the atmosphere. With these high statistics extending to lower energies than any previous experiment, and with complementary sources of experimental uncertainty compared to traditional magnetic spectrometers, the GAPS antiproton measurement will be sensitive to dark matter, primordial black holes, and cosmic ray propagation. The antiproton measurement will also validate the GAPS antinucleus identification technique for the antideuteron and antihelium rare-event searches. This analysis demonstrates the GAPS sensitivity to cosmic-ray antiprotons using a full instrument simulation and event reconstruction, and including solar and atmospheric effects.
Microbial pathogens strategically acquire metabolites from their hosts during infection. Here we show that the host can intervene to prevent such metabolite loss to pathogens. ...Phosphorylation-dependent regulation of sugar transport protein 13 (STP13) is required for antibacterial defense in the plant Arabidopsis thaliana. STP13 physically associates with the flagellin receptor flagellin-sensitive 2 (FLS2) and its co-receptor BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 (BAK1). BAK1 phosphorylates STP13 at threonine 485, which enhances its monosaccharide uptake activity to compete with bacteria for extracellular sugars. Limiting the availability of extracellular sugar deprives bacteria of an energy source and restricts virulence factor delivery. Our results reveal that control of sugar uptake, managed by regulation of a host sugar transporter, is a defense strategy deployed against microbial infection. Competition for sugar thus shapes host-pathogen interactions.
Aim: Risk of cardiovascular disease is increased in patients with diabetes mellitus (DM). Cholesterol metabolism (hepatic synthesis and intestinal absorption) is known to be associated with ...cardiovascular risk. Next, we examined the association of DM with cholesterol absorption/synthesis.Methods: The CACHE Consortium, which is comprised of 13 research groups in Japan possessing data of lathosterol (Latho, synthesis marker) and campesterol (Campe, absorption marker) measured by gas chromatography, compiled the clinical data using the REDCap system. Among the 3597 records, data from 2944 individuals were used for several analyses including this study. Results: This study analyzed data from eligible 2182 individuals including 830 patients with DM; 42.2% were female, median age was 59 years, and median HbA1c of patients with DM was 7.0%. There was no difference in Latho between DM and non-DM individuals. Campe and Campe/Latho ratio were significantly lower in DM individuals than in non-DM individuals. When the associations of glycemic control markers with these markers were analyzed with multivariable-adjusted regression model using restricted cubic splines, Campe and Campe/Latho ratio showed inverse associations with glucose levels and HbA1c. However, Latho showed an inverted U-shaped association with plasma glucose, whereas Latho showed a U-shaped association with HbA1c. These associations remained even after excluding statin and/or ezetimibe users.Conclusion: We demonstrated that DM and hyperglycemia were independent factors for lower cholesterol absorption marker levels regardless of statin/ezetimibe use.
Aim: Blood cholesterol absorption and synthesis biomarkers predict cardiovascular risk. This study aimed to determine the values of serum non-cholesterol sterol markers lathosterol (Latho), ...campesterol (Campe), and sitosterol (Sito) in healthy individuals and factors affecting these markers.Methods: The CACHE Consortium compiled clinical data, including serum Latho (cholesterol synthesis marker), and Campe and Sito (cholesterol absorption markers), by a gas chromatography method in 2944 individuals. Healthy subjects were selected by excluding those with prior cardiovascular disease, diabetes mellitus, hypertension, chronic kidney disease, familial hypercholesterolemia, sitosterolemia, current smokers, those with low (＜17 kg/m2) or high (≥ 30 kg/m2) body mass index (BMI), and those with treatment for dyslipidemia or hyperuricemia. Nonlinear regression stratified by sex was used to examine the associations of cholesterol metabolism markers with age, BMI, and serum lipid levels.Results: Of 479 individuals selected, 59.4% were female; the median age was 48 years in females and 50 years in males. The three markers showed positively skewed distributions, and sex differences were present. Age was associated positively with Latho, inversely with Campe, but not significantly with Sito. BMI was associated positively with Latho, but not significantly with Campe or Sito. High-density lipoprotein cholesterol (HDL-C) was positively associated with Campe and Sito, but not significantly with Latho. Non-HDL-C was positively associated with the three markers.Conclusion: Our study results in the healthy subjects help to interpret the non-cholesterol sterol markers for cardiovascular risk assessment in patients with cardiovascular risk factors.
Aim: Serum levels of cholesterol absorption and synthesis markers are known to be associated with cardiovascular risk. Individuals with reduced kidney function or chronic kidney disease (CKD) are at ...an increased risk for cardiovascular disease. Hence, we examined the relationship between estimated glomerular filtration rate (eGFR) and serum markers of cholesterol absorption and synthesis.Methods: The CACHE (Cholesterol Absorption and Cholesterol synthesis in High-risk patiEnts) Consortium, comprised of 13 research groups in Japan possessing data of lathosterol (Latho, synthesis marker) and campesterol (Campe, absorption marker) measured via gas chromatography, compiled the clinical data using the REDCap system. Among the 3597 records, data from 2944 individuals were utilized for five analyses including this CKD analysis.Results: This study analyzed data from 2200 individuals including 522 hemodialysis patients; 42.3% were female, the median age was 58 years, and the median eGFR was 68.9 mL/min/1.73 m2. Latho, Campe, and Campe/Latho ratio were significantly different when compared across CKD stages. When the associations of eGFR with these markers were assessed with multivariable nonlinear regression models, Latho, Campe, and Campe/Latho ratio showed positive, inverse, and inverse associations with eGFR. These associations were significantly modified by sex, the presence/absence of diabetes mellitus, and the presence/absence of statin use.Conclusion: We showed that individuals with lower eGFR have lower cholesterol synthesis marker levels and higher cholesterol absorption marker levels in this large sample.
Aim: Serum levels of cholesterol absorption and synthesis markers are known to be associated with cardiovascular risk. Familial hypercholesterolemia (FH) is a well-known inherited disorder presenting ...elevated low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) levels and premature coronary disease. In this study, we aim to examine the differences in terms of serum markers of cholesterol metabolism between FH and non-FH individuals and to examine their associations with serum lipid levels.Methods: In this study, we utilized data on serum markers of cholesterol metabolism, namely, lathosterol (Latho, synthesis marker), campesterol (Campe, absorption marker), and sitosterol (Sito, absorption marker) measured by gas chromatography of the CACHE consortium, which comprised of 13 research groups in Japan. Clinical data were compiled using REDCap system. Among the 2944 individuals in the CACHE population, we selected individuals without lipid-lowering medications and hemodialysis patients for this CACHE study FH analysis. Multivariable adjustment was performed to assess the associations. Results: In this study, we analyzed data from 51 FH patients and 1924 non-FH individuals. After adjustment for possible confounders, the FH group was shown to have significantly higher Campe and Sito concentrations and insignificantly higher Latho concentrations than the non-FH group. These marker concentrations showed nonlinear associations with TC in the FH group. Campe/Latho and Sito/Latho ratios were significantly higher in the FH group than in the non-FH group.Conclusion: FH group had significantly elevated serum Campe and Sito concentrations and insignificantly elevated Latho concentrations; thus, intestinal cholesterol absorption relative to hepatic cholesterol synthesis was suggested to be elevated in patients with FH. Serum Latho, Campe, and Sito concentrations showed nonlinear associations with TC in the FH group.
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