Biomarker testing has gradually become standard of care in precision oncology to help physicians select optimal treatment for patients. Compared to single-gene or small gene panel testing, ...comprehensive genomic profiling (CGP) has emerged as a more time- and tissue-efficient method. This study demonstrated in-depth analytical validation of K-4CARE, a CGP assay that integrates circulating tumor DNA (ctDNA) tracking for residual cancer surveillance.
The assay utilized a panel of 473 cancer-relevant genes with a total length of 1.7 Mb. Reference standards were used to evaluate limit of detection (LOD), concordance, sensitivity, specificity and precision of the assay to detect single nucleotide variants (SNVs), small insertion/deletions (Indels), gene amplification and fusion, microsatellite instability (MSI) and tumor mutational burden (TMB). The assay was then benchmarked against orthogonal methods using 155 clinical samples from 10 cancer types. In selected cancers, top tumor-derived somatic mutations, as ranked by our proprietary algorithm, were used to detect ctDNA in the plasma.
For detection of somatic SNVs and Indels, gene fusion and amplification, the assay had sensitivity of >99%, 94% and >99% respectively, and specificity of >99%. Detection of germline variants also achieved sensitivity and specificity of >99%. For TMB measurement, the correlation coefficient between whole-exome sequencing and our targeted panel was 97%. MSI analysis when benchmarked against polymerase chain reaction method showed sensitivity of 94% and specificity of >99%. The concordance between our assay and the TruSight Oncology 500 assay for detection of somatic variants, TMB and MSI measurement was 100%, 89%, and 98% respectively. When CGP-informed mutations were used to personalize ctDNA tracking, the detection rate of ctDNA in liquid biopsy was 79%, and clinical utility in cancer surveillance was demonstrated in 2 case studies.
K-4CARE™ assay provides comprehensive and reliable genomic information that fulfills all guideline-based biomarker testing for both targeted therapy and immunotherapy. Integration of ctDNA tracking helps clinicians to further monitor treatment response and ultimately provide well-rounded care to cancer patients.
Hereditary cancer syndromes (HCS) are responsible for 5-10% of cancer cases. Genetic testing to identify pathogenic variants associated with cancer predisposition has not been routinely available in ...Vietnam. Consequently, the prevalence and genetic landscape of HCS remain unknown.
1165 Vietnamese individuals enrolled in genetic testing at our laboratory in 2020. We performed analysis of germline mutations in 17 high- and moderate- penetrance genes associated with HCS by next generation sequencing.
A total of 41 pathogenic variants in 11 genes were detected in 3.2% individuals. The carrier frequency was 4.2% in people with family or personal history of cancer and 2.6% in those without history. The percentage of mutation carriers for hereditary colorectal cancer syndromes was 1.3% and for hereditary breast and ovarian cancer syndrome was 1.6%.
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mutations were the most prevalent with the positive rate of 1.3% in the general cohort and 5.1% in breast or ovarian cancer patients. Most of
mutations located at the BRCA C-terminus domains and the top recurrent mutation was NM_007294.3:c.5251C>T (p.Arg1751Ter). One novel variant NM_000038.6(APC):c.6665C>A (p.Pro2222His) was found in a breast cancer patient with a strong family history of cancer. A case study of hereditary cancer syndrome was illustrated to highlight the importance of genetic testing.
This is the first largest analysis of carrier frequency and mutation spectrum of HCS in Vietnam. The findings demonstrate the clinical significance of multigene panel testing to identify carriers and their at-risk relatives for better cancer surveillance and management strategies.
Due to the low melting temperature, the glazes based on the Bi2O3-B2O3-ZnO system are used as coatings on the surface of industrial glass substrates. Moreover, the composition of these coatings does ...not contain PbO, meeting the optical and environmental properties requirements. In this study, TiO2 was used in the Bi2O3-B2O3-ZnO glaze system to improve its photocatalytic ability. This can be considered a four – component glass system Bi2O3-B2O3-ZnO-TiO2. The heating microscopy results show that the melting temperature of the glaze system is 606 °C. The Fourier transform infrared spectroscopy results show that the TiO2 polyhedra are located independently in the structure without participating in forming a glass network. Thanks to that, the photocatalytic properties of TiO2 are maintained. The X-ray diffraction patterns results show that the formed TiO2 nanocrystals are rutile and anatase crystals. The results of determining the band gap energy using UV-Vis show that the band gap energy of the base glaze system increases with the addition of TiO2. The methylene blue decomposition results also showed that the ability to decompose organic increased when TiO2 was added to the glaze coating. The characteristics such as melting temperature, microstructure, and photocatalytic capacity of Bi2O3-B2O3-ZnO-TiO2 white glazes (5 and 10 % weight of TiO2) also were indicated in this paper.
This paper introduces a method for producing red copper glaze by adding copper oxide (CuO) and silicon carbide (SiC) additives to the base glaze. SiC created a reducing environment in situ and ...allowed the glaze to be sintered in an oxidizing furnace environment. Nanocrystals are the determinants of the red color of the glaze. The CuO reduction reaction temperature range of SiC produces a reducing environment in the glaze as detected by the method (DSC). The functional group and phase of nanocrystals were determined by Fourier transform infrared (FT-IR) and X-ray diffraction (XRD) spectroscopy.
Calcium silicate is produced from a mixture of silica sand powder, lime, paper pulp, and Portland cement hydrothermally steamed at 180 °C for about 16 h. This material is considered environmentally ...friendly and is popular in countries around the world. In this study, quartz sand was replaced with residual waste sludge from water-filtration plants to produce calcium silicate materials. Nowadays, the residual waste sludge from water-filtration plants is an environmental problem that needs to be treated. The results of determining the properties showed that a sample using 10 w/% residual waste sludge gave the best replacement. This sample had a bending strength of 10.95 MPa, a volumetric density of 1.57 g/cm3, and water absorption of 23.67 %. The results of the analysis of the mineral composition (by X-ray diffraction analysis and Fourier-transform infrared spectroscopy) and microstructure (by scanning electron microscopy) showed that all samples formed tobermorite and xonotlite minerals. The tobermorite and the xonotlite are hydro-silicate-calcium minerals characteristic of calcium silicate materials, which are the synthesis products of chemical reactions of SiO2, CaO, and H2O under hydrothermal conditions. Samples using 5–10 w/% of waste residual sludge have even higher mechanical strength than samples without. Therefore, using waste residual sludge from water-filtration plants to replace part of the sand in producing calcium silicate materials can be considered an effective method to treat environmental problems caused by waste residual sludge.
Treatment of the sludge from water-purification plants is becoming more and more urgent due to the inability to increase its storage area. To avoid CO2 emissions, the use of non-Portland cement ...binders is recommended. The application of geopolymerization of waste sludge (WS) from water-purification plants is a novel solution. Curing conditions including high temperature, pressure or microwaves enhance the formation of geopolymer bonds. This paper presents the results of a research on the treatment of the WS of the Thu Duc water-purification plant (Vietnam) with the geopolymerization method. Solid phases were prepared by mixing the WS and fly ash (FA). The FA proportions of the solid phases were (10, 40, 70) w/%. The alkali-activated solution (AAS) was a mixture of a 40 w/% NaOH 6M solution and 60 w/% water glass (WG: Na2O.nSiO2 with n = 1.75 and volumetric density r = 1.40 kg/L). The geopolymer materials were mixtures containing an 80 w/% solid phase and a 20 w/% liquid phase of the AAS. Geopolymer samples were formed in a cylindrical steel mold with a diameter of 10 mm at a high pressure. The samples were cured in a 112 W microwave oven for 30 s or in a dryer at 110 °C for 24 h. The compressive strength and volumetric density of both sample groups were determined and compared to each other. The formation of geopolymer bonds was investigated using XRD, FTIR and SEM.
Lead-free Bi2O3 - B2O3 - ZnO glass is a commonly synthesized glass system at low temperatures for coating applications formaterials such as ceramic, glass, metal, etc. TiO2 or Ag/TiO2 composite are ...usually photocatalyst for antimicrobial application.
In this study, TiO2 or Ag/TiO2 were combined with the Bi2O3 - B2O3 - ZnO glass system by the technique of rapid cooling ofmolten glass at 1200oC. The influence of the TiO2 or Ag/TiO2 on the optical characteristics of the Bi2O3 - B2O3 - ZnO glasssystem was performed by using X-ray diffraction, Fourier Transform Infrared Spectroscopy, and Ultraviolet-VisibleSpectroscopy techniques. The analytical results showed that the band gap energy of the Bi2O3 - B2O3 - ZnO glass system hada low value. The band gap energy of the system increased with the addition of TiO2 and decreased slightly with the additionof Ag/TiO2. In addition, when Ti2O or Ag/TiO2 were added, the glass system appeared crystals such as anatase, rutile and silveroxide on an amorphous background. These crystals cause the system's transmittance to decrease and reflectance to increase.
However, the appearance of these crystals promises that Bi2O3 - B2O3 - ZnO glass materials can be applied as antibacterialmaterials. The antibacterial property is due to the photocatalytic effect of TiO2 and the ability to kill bacteria Ag2O. KCI Citation Count: 0
Rice husk is a waste product of agricultural production of rice. Every year, the agricultural production of Vietnam emits about10 million tons of rice husks. Rice husks are usually treated by burning ...method or discharged into rivers, causing manynegative impacts on the environment. In this study, the rice husk/silica gel ratio components were investigated to form SiC/SiO2-C composite in a CO2 environment at 1000 oC for 60 min. The products after pyrolysis were determined SiC, SiO2, andfree C contents according to ISO 21068-2: 2008 standards. The functional groups and mineral composition of composites wereanalyzed by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The microstructure and elementdistribution of them were observed by scanning electron microscope (SEM) and Energy-dispersive X-ray (EDX) mapping. Theresults showed that the optimum rice husk/silica gel ratio to form SiC/SiO2-C was 1.4/1. The sample after pyrolysis hadchemical composition including 14% SiC, 63% SiO2, 23% free C (%wt.) and the phase composition including amorphouscarbon, β-SiC, 6H-SiC, 4H-SiC, cristobalite. Composite SiC/SiO2-C can apply to advanced friction systems. This can be seenas an effective method to treat rice husk waste and improved the economic value of rice plants. KCI Citation Count: 0
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