The use of probiotics is a strategy employed to improve host health status and to prevent infectious diseases. The current study was aimed at investigating the diversity of lactic acid bacteria (LAB) ...and Bacillus species in the gastrointestinal tracts of wild marine fishes, as well as the beneficial effects of Lactococcus lactis WFLU12 as a host-derived probiotics in olive flounder. In marine fishes, wild olive flounder and rock bream were shown to be good sources of LAB and Bacillus isolation, respectively. Some isolates, including the strain WFLU12, have shown stronger inhibitory activity against various aquatic bacterial pathogens and more tolerance to low pH and bile acids compared to some strains isolated from sources other than marine. Lc. lactis WFLU12 was found to confer to olive flounder protection against streptococcosis caused by Streptococcus parauberis through competitive exclusion and increased innate immune responses. Interestingly, the natural infection rate in the probiotic fed group (33%=10/30) was significantly lower than that in the control group (60%=18/30). None of the nisin Z and colicin V-producing probiotic-fed fish were naturally infected by S. parauberis during the feeding period. In addition, more importantly, this promising probiotic strain significantly promoted fish growth along with better feed conversion and specific growth rate. This study demonstrates that the use of host-derived probiotics can offer a significant advantage in terms of optimum survival and function in the gastrointestinal tract of the intended host.
In this study, host-derived probiotic strain outperforms elimination of pathogen through competitive exclusion in the gastrointestinal tract and increased innate immune responses. More importantly, this promising probiotic strain significantly promoted fish growth along with better feed conversion. This study will provide insight into how optimal probiotics should be selected and developed. It might facilitate the replacement of commercial fish probiotic products originated from terrestrial sources with host-derived probiotics in the near future.
•From the gastrointestinal tract of wild fish, various lactic acid bacteria (LAB) and bacillus were isolated and explored.•Host-derived probiotic, Lc. lactis WFLU12 was found to confer to olive flounder protection against streptococcosis disease.•Examination of natural infection and artificial in vivo screening might be included in a selection of probiotic candidate.•Preliminary genome analysis showed consistent genes encoding for antagonistic activity and viable ability in fish intestine.
Mechanisms of Pulmonary Vein Reconnection After Radiofrequency Ablation of Atrial Fibrillation
Introduction
Pulmonary vein reconnection (PVR) is an important cause of AF recurrence after ablation. ...With the advent of force sensing catheters, catheter–tissue contact can be determined quantitatively. Since contact force (CF) plays a major role in determining the characteristics of RF lesion, we prospectively assessed the mechanisms of PVR with regard to catheter‐contact and lesion distances in patients undergoing AF ablation.
Methods and Results
Forty symptomatic AF patients underwent wide circumferential PV isolation (PVI) with SmartTouch™ CF catheter. The exact locations of acute PVI and spontaneous or adenosine‐provoked PVR were annotated on CARTO. One thousand nine hundred and twenty‐six RF lesions isolated 153 PVs. PVR occurred in 35 (23%) PVs: 22 (63%) adenosine‐provoked and 13 (37%) spontaneous. CF was significantly lower at PVR versus PVI sites for RF lesions within 6 mm from these sites: mean CF 5 versus 11 g (P < 0.0001) and force–time integral (FTI) 225 versus 415 gs (P < 0.0001); 86% of PVR occurred with a mean CF < 10 g (FTI < 400 gs); and the remaining 14% occurred at ablation sites with a long interlesion distance (≥5 mm) despite mean CF ≥ 10 g. Eighty percent of PVR sites were located anteriorly. There were no significant differences in regard to arrhythmia freedom between the patients without (69%) versus with PVR (67%; P = 1.0).
Conclusions
Acutely durable PVI can be achieved when RF lesions are delivered with a mean CF ≥ 10 g and an interlesion distance <5 mm. The majority of PVR occur anteriorly due to inadequate CF or long interlesion distances.
Malignant melanoma accounts for about 1–3% of all malignancies in the West, especially in the United States. More than 9000 people die each year. In general, it is difficult to characterize a skin ...lesion from a photograph. In this paper, we propose a deep learning-based computer-aided diagnostic algorithm for the classification of malignant melanoma and benign skin tumors from RGB channel skin images. The proposed deep learning model constitutes a tumor lesion segmentation model and a classification model of malignant melanoma. First, U-Net was used to classify skin lesions in dermoscopy images. We implement an algorithm to classify malignant melanoma and benign tumors using skin lesion images and expert labeling results from convolutional neural networks. The U-Net model achieved a dice similarity coefficient of 81.1% compared to the expert labeling results. The classification accuracy of malignant melanoma reached 80.06%. As a result, the proposed AI algorithm is expected to be utilized as a computer-aided diagnostic algorithm to help early detection of malignant melanoma.
Although Carnobacterium maltaromaticum has been used as a probiotic in fish, it was reported to cause disease for the first time in Korea. The objective of this study was to understand the ...differences between pathogenic and non‐pathogenic strains. Pathogenicity was tested by challenging rainbow trout with C. maltaromaticum ATCC35586 and 18ISCm isolated from diseased fish, and DSM20342 isolated from a dairy product. We also compared 24 genomes of C. maltaromaticum strains plus the genome of our isolate 18ISCm sequenced in this study. Only the strains from diseased fish caused high mortality with severe histopathological changes. Although all strains shared more than 90% of Ko_id, wecC and xtmA were found only in strains from diseased fish. Interestingly, only strains from diseased fish harboured two wecC paralogs involved in the production of D‐mannosaminuronic acid which is a major component of a well‐known virulence factor, teichuronic acid. Two wecC paralogs of 18ISCm were increased when they were co‐cultured with trout blood cells, suggesting that wecC genes might play a role in virulence. The results of this study show that strains isolated from diseased fish are different from strains derived from food in terms of pathogenicity to fish and the presence of virulence‐related genes.
Red sea bream iridovirus (RSIV) is an important aquatic virus that causes high mortality in marine fish. RSIV infection mainly spreads through horizontal transmission via seawater, and its early ...detection could help prevent disease outbreaks. Although quantitative PCR (qPCR) is a sensitive and rapid method for detecting RSIV, it cannot differentiate between infectious and inactive viruses. Here, we aimed to develop a viability qPCR assay based on propidium monoazide (PMAxx), which is a photoactive dye that penetrates damaged viral particles and binds to viral DNA to prevent qPCR amplification, to distinguish between infectious and inactive viruses effectively. Our results demonstrated that PMAxx at 75 μM effectively inhibited the amplification of heat-inactivated RSIV in viability qPCR, allowing the discrimination of inactive and infectious RSIV. Furthermore, the PMAxx-based viability qPCR assay selectively detected the infectious RSIV in seawater more efficiently than the conventional qPCR and cell culture methods. The reported viability qPCR method will help prevent the overestimation of red sea bream iridoviral disease caused by RSIV. Furthermore, this non-invasive method will aid in establishing a disease prediction system and in epidemiological analysis using seawater.
Viral hemorrhagic septicemia virus (VHSV) is a rhabdovirus that causes high mortality in cultured flounder. Naturally occurring VHSV strains vary greatly in virulence. Until now, little has been ...known about genetic alterations that affect the virulence of VHSV in flounder. We recently reported the full-genome sequences of 18 VHSV strains. In this study, we determined the virulence of these 18 VHSV strains in flounder and then the assessed relationships between differences in the amino acid sequences of the 18 VHSV strains and their virulence to flounder. We identified one amino acid substitution in the phosphoprotein (P) (Pro55-to-Leu substitution in the P protein; PP55L) that is specific to highly virulent strains. This PP55L substitution was maintained stably after 30 cell passages. To investigate the effects of the PP55L substitution on VHSV virulence in flounder, we generated a recombinant VHSV carrying PP55L (rVHSV-P) from rVHSV carrying P55 in the P protein (rVHSV-wild). The rVHSV-P produced high level of viral RNA in cells and showed increased growth in cultured cells and virulence in flounder compared to the rVHSV-wild. In addition, rVHSV-P significantly inhibited the induction of the IFN1 gene in both cells and fish at 6 h post-infection. An RNA-seq analysis confirmed that rVHSV-P infection blocked the induction of several IFN-related genes in virus-infected cells at 6 h post-infection compared to rVHSV-wild. Ectopic expression of PP55L protein resulted in a decrease in IFN induction and an increase in viral RNA synthesis in rVHSV-wild-infected cells. Taken together, our results are the first to identify that the P55L substitution in the P protein enhances VHSV virulence in flounder. The data from this study add to the knowledge of VHSV virulence in flounder and could benefit VHSV surveillance efforts and the generation of a VHSV vaccine.
To investigate the pattern of nodal metastasis, morbidity, recurrence rates of papillary thyroid carcinoma (PTC), and parathyroid hormone (PTH) responses following neck dissection (ND) plus total ...thyroidectomy (TT).
While hypoparathyroidism develops after TT plus ND, little is known of postoperative PTH response.
Of 155 PTC patients, 82 underwent TT plus bilateral central ND with/without lateral ND, while 73 underwent TT alone. The nodal metastasis pattern was determined and the recurrence, morbidity, and postoperative levels of serum calcium and PTH were compared between 2 groups.
Of the 82 node dissection patients, metastatic nodes were present in the central neck of 51 (62.2%) and the lateral neck of 21 (25.6%) patients, most frequently in the ipsilateral and pretracheal central nodes and lateral jugular nodes. Four regional recurrences (2.6%) were found in 3 patients of the no node dissection group and one of the node dissection group (P = 0.37) during the follow-up lasting a mean 52 months. Overall morbidity and hypocalcemia was higher in the node dissection group than the no node dissection group (41 of 82, 50%; vs. 9 of 73, 12.3%; P < 0.001; 25 of 82, 30.5%; vs. 7 of 73, 9.6%; P = 0.001). Serum PTH levels significantly decreased immediately postoperatively in the node dissection group and remained low for several weeks thereafter.
Serum PTH levels were significantly reduced following ND in PTC patients. Our data suggest that, when performing therapeutic ND plus TT, particular effort should be made to preserve the parathyroid glands and to monitor their function.
Rock bream iridovirus (RBIV) is a notorious agent that causes high mortality in aquaculture of rock bream (
). Despite severity of this virus, no transcriptomic studies on RBIV-infected rock bream ...that can provide fundamental information on protective mechanism against the virus have been reported so far. This study aimed to investigate physiological mechanisms between host and RBIV through transcriptomic changes in the spleen based on RNA-seq. Depending on infection intensity and sampling time point, fish were divided into five groups: uninfected healthy fish at week 0 as control (0C), heavy infected fish at week 0 (0H), heavy mixed RBIV and bacterial infected fish at week 0 (0MH), uninfected healthy fish at week 3 (3C), and light infected fish at week 3 (3L). We explored clusters from 35,861 genes with Fragments Per Kilo-base of exon per Million mapped fragments (FPKM) values of 0.01 or more through signed co-expression network analysis using WGCNA package. Nine of 22 modules were highly correlated with viral infection (|gene significance (GS) vs. module membership (MM) |> 0.5,
-value < 0.05). Expression patterns in selected modules were divided into two: heavy infected (0H and 0MH) and control and light-infected groups (0C, 3C, and 3L). In functional analysis, genes in two positive modules (5448 unigenes) were enriched in cell cycle, DNA replication, transcription, and translation, and increased glycolysis activity. Seven negative modules (3517 unigenes) built in this study showed significant decreases in the expression of genes in lymphocyte-mediated immune system, antigen presentation, and platelet activation, whereas there was significant increased expression of endogenous apoptosis-related genes. These changes lead to RBIV proliferation and failure of host defense, and suggests the importance of blood cells such as thrombocytes and B cells in rock bream in RBIV infection. Interestingly, a hub gene, pre-mRNA processing factor 19 (PRPF19) showing high connectivity (kME), and expression of this gene using qRT-PCR was increased in rock bream blood cells shortly after RBIV was added. It might be a potential biomarker for diagnosis and vaccine studies in rock bream against RBIV. This transcriptome approach and our findings provide new insight into the understanding of global rock bream-RBIV interactions including immune and pathogenesis mechanisms.
Due to the outbreak of new viruses in recent years, the need for contactless medical systems has significantly increased for monitoring, treating, managing, and preventing diseases. Systems are ...constantly being developed to replace conventional biosignal measuring methods with contactless medical systems. Several systems that substitute attachable sensors in conventional systems have been developed to measure diverse biosignals using images captured with visible cameras. Most non-contact biometric systems have certain limitations, for example, they can only measure a single biosignal, such as the heart rate, oxygen saturation, or blood pressure. They are also easily affected by external factors such as lighting conditions and noises. Furthermore, a blood pressure measuring system based on the pulse transit time requires physical contact and accuracy depends on the measurement location. This study examined a system that provides highly accurate biosignals to overcome these limitations of conventional biosignal measurement systems. This study proposes a biosignal measurement system that uses an infra-red camera to reduce visible light noise. The proposed system uses light sources outside the visible range and measures the amplified reflected light using a near infra-red camera to calculate the heart rate and oxygen saturation. The blood flow value is calculated by the measured heart rate and oxygen saturation. Based on measured data, blood pressure is estimated without contact with the body. We compared the proposed system with an existing non-contact blood pressure measurement system. The proposed system showed an average error rate of 2.07% in blood pressure measurement, which increased by 3.86% compared to the conventional system. Also, this study enabled the early detection of pressure ulcers, which is difficult to measure quantitatively.
PDF
