Objetivos: Investigar os desfechos perinatais de gestantes com diagnóstico de pré-eclâmpsia grave. Métodos: Estudo transversal desenvolvido em uma maternidade-escola, com amostra de 157 ...fetos/neonatos de gestantes com diagnóstico de pré-eclâmpsia grave. Para análise dos dados foram calculados os valores de estatísticas descritiva com uso do SPSS 21.0. Resultados: Em 22,3% dos casos a gestação evoluiu com diagnóstico de restrição de crescimento intra-uterino. O óbito fetal teve incidência de 7,6%. Dos neonatos, 48% nasceram com idade inferior a 37 semanas e mais da metade (56,7%) foram classificados como de baixo peso. O índice de APGAR no primeiro e quinto minutos foram compatíveis com anóxia moderada. 20,4% dos recém-nascidos necessitaram de reanimação e 18,5% foram admitidos na unidade de terapia intensiva neonatal. Conclusões: A pré-eclâmpsia repercutiu com a presença da restrição de crescimento intrauterino, óbito fetal, prematuridade, baixo peso, necessidade de reanimação neonatal e admissão na unidade de cuidados intensivos.
Miocardiopatia de Takotsubo pós-parto: relato de caso Sousa, Vera Cristina Pereira de; Pinto, Ana Patrícia Lopes; Lopes, Ana Paula Calado ...
Revista Brasileira de ginecologia e obstetrícia,
11/2015, Letnik:
37, Številka:
11
Journal Article
Recenzirano
Odprti dostop
Ocorrendo habitualmente após um stress físico ou emocional intenso, a miocardiopatia de Takotsubo caracteriza-se por disfunção aguda e transitória do segmento apical do ventrículo esquerdo, ...mimetizando uma síndrome coronária aguda. Essa é uma síndrome rara, na qual o diagnóstico diferencial assume particular importância. Um elevado nível de suspeição é essencial, pelo que obstetras e o restante da equipe devem estar preparados para diagnosticar e lidar com esse evento inesperado. O tratamento é fundamentalmente de suporte, verificando-se reversão espontânea e completa das alterações num intervalo de dias a semanas. A ocorrência de complicações podem ditar um prognóstico menos benigno. Apresentamos o caso de uma mulher de 39 anos, sem antecedentes relevantes. Submetida à cesariana por suspeita de sofrimento fetal. Terminada a intervenção iniciou quadro de bradicardia e precordialgia, com edema pulmonar. Apresentava alterações de enzimas cardíacas e do electrocardiograma. O ecocardiograma revelou disfunção sistólica do ventrículo esquerdo com hipocinésia de toda a parede anterior. O cateterismo cardíaco excluiu doença coronária obstrutiva.
The repertoire of microRNAs (miRNAs) in thyroid carcinomas starts to be elucidated. Among differentiated thyroid carcinomas (DTCs), papillary thyroid carcinoma (PTC) is the most frequent. The ...assessment of miRNAs expression may contribute to refine the pre-surgical diagnosis in order to obtain a personalized and more effective treatment for patients.
This study aims to evaluate (1) the miRNAs in a series of DTCs, and their association with the presence of selected genetic mutations in order to improve diagnosis and predict the biologic behavior of DTC/PTC. (2) The reliability of molecular tests in Ultrasound-guided Fine Needle Aspiration Cytology (US-FNAC) for a more precise preoperative diagnosis.
This series includes 176 samples (98 cytology and 78 histology samples) obtained from 106 patients submitted to surgery, including 13 benign lesions (controls) and 93 DTCs (cases). The microRNA expression was assessed for miR-146b, miR-221, miR-222, and miR-15a through quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). The results were analyzed by the 2
method, using miR16 as an endogenous control. Regarding PTC diagnosis, the discriminative ability of miRNAs expression was assessed by the area under the Receiver Operating Characteristic Curve (AUC). In PTCs, the association of miRNAs expression, clinicopathological features, and genetic mutations (
,
and
) was evaluated.
All the analyzed miRNAs presented a tendency to be overexpressed in DTCs/PTCs when compared with benign lesions, both in cytology and histology samples. In cytology, miRNAs expression levels were higher in malignant tumors than in benign tumors. In histology, the discriminative abilities regarding PTC diagnosis were as follows: miR-146b (AUC 0.94, 95% CI 0.87-1), miR-221 (AUC 0.79, 95% CI 0.68-0.9), miR-222 (AUC 0.76, 95% CI 0.63-0.89), and miR-15a (AUC 0.85, 95% CI 0.74-0.97). miR-146b showed 89% sensitivity (se) and 87% specificity (sp); miR-221 se = 68.4, sp = 90; miR-222 se = 73, sp = 70; and mi-R15a se = 72, sp = 80. MicroRNAs were associated with worst-prognosis clinicopathological characteristics in PTCs (
< 0.05), particularly for miR-222. Our data reveal a significant association between higher expression levels of miR-146b, miR-221, and miR-222 in the presence of the
mutation (
< 0.001) and miR-146b (
= 0.016) and miR-221 (
= 0.010) with the
mutation, suggesting an interplay of these mutations with miRNAs expression. Despite this study having a relatively small sample size, overexpression of miRNAs in cytology may contribute to a more precise preoperative diagnosis. The miRNAs presented a good discriminative ability in PTC diagnosis. The association between the miRNAs expression profile and genetic alterations can be advantageous for an accurate diagnosis of DTCs/PTCs in FNAC.
Rabbit production holds significant relevance in modern agriculture due to its potential as a sustainable source of high-quality protein and efficient feed conversion, contributing to food security ...and economic diversification. Nevertheless, studies incorporating feto-maternal monitoring in this species are uncommon. This review gathers research on the monitoring and evaluation of factors affecting rabbit gestation, providing a better understanding of the causes of prenatal development abnormalities. These include studies regarding how chronic maternal hypertension, gestational diabetes, maternal stress, ectopic gestation, maternal uterine ischemia and fetal hypoxia, intrauterine growth restriction, superfetation, maternal age, maternal nutritional status, maternal physical condition, maternal and embryonic genotype, and the intrauterine location of rabbit fetuses can potentially impact rabbits’ reproduction and maternal and fetal health. Among other monitoring techniques, ultrasonography, considered one of the best tools for diagnosing pregnancy and conducting follow-up, is also reviewed. Details on measurable fetal-development parameters in rabbits and precautions to be considered before and during the examination are also provided. Additional studies are required to understand why some events occur and their consequences throughout gestation, allowing the determination of new biomarkers or cut-offs that can be helpful for early diagnosis and improve reproductive efficiency.
Introduction: A bioterrorist attack is the intentional release of pathogenic micro-organisms, such as viruses, bacteria, or their toxins, with the aim of causing illness or death in people, animals, ...or plants. In this study, we investigated the knowledge and practices related to bioterrorism preparedness in Central Portugal. Methods: A descriptive cross-sectional study was performed with a convenience sample in the population of Aveiro, Central Portugal, to assess their knowledge about bioterrorism, self-perceived preparation to act in case of bioterrorism and pet owners’ preparation. An online validated questionnaire was completed by 198 participants from January to February 2020. Results: In this study, 46.0% of the respondents answered that they knew nothing about bioterrorism or had never heard about the possibility of bioterrorist attacks. In the case of an attack, 77.8% participants did not consider themselves prepared to act, and 62.1% did not know how to use personal protective equipment. More than half of the respondents (60.6%) were not familiar with the local emergency response system in response to catastrophes/bioterrorist attacks. Almost all respondents (95.6%) assigned high importance to drinking water and food for pets, but only 22.9% of respondents attributed high importance to pet carrier boxes, an item essential for cat evacuation. Conclusion: This is the first survey of this kind in Portugal concerning bioterrorism preparedness in citizens and animals. Results suggest that Portuguese knowledge is limited, and people have inadequate preparedness for a bioterrorist attack. These results reinforce the importance of further studies to better understand the existing gaps in knowledge of Portuguese citizens, strengthen the need to adopt the One Health concept in preparedness plans and emphasize the crucial role of health education in prevention.
Paratuberculosis, or Johne's disease, caused by
subsp.
(MAP), is a chronic granulomatous enteritis affecting both domestic and wild ruminants. The agent was also found in wild mammals such as wild ...boar (
); however, the role of wild mammals in the epidemiology of MAP is unclear. During the research period, 941 free-ranging wild boar (
) legally hunted in two locations in the central-eastern region of Portugal were examined. Ninety-seven wild boars exhibited one or more gross lesions and were tested for the presence of
subsp.
using acid-fast staining, mycobacterial culture, polymerase chain reaction (PCR), and histopathological examination. Forty-five animals (46.4%, 95% CI: 36.5-56.3%) were identified as infected, as indicated by positive results in culture and/or PCR. The findings revealed that the most significant risk factor was being a juvenile compared to yearlings and adults (OR = 10.2, 95% CI: 2.2-48.0). Based on our results, 37.9% (
= 11) of the infected animals were considered suitable for human consumption. Our findings offer novel insights into mycobacterial infections in wild boar populations in Portugal and suggest that wild boar could be a source of human infection if zoonotic potential is considered.
The African Grey parrot (Psittacus erithacus) is one of the most popular avian pets in exotic animal practice. In this study, blood samples (n = 459) from healthy African Grey parrots (n = 239) were ...collected for haematological testing. Reference intervals were determined according to the guidelines of the American Society of Veterinary Clinical Pathology with the Reference Value Advisor (v2.1) freeware. The calculated reference intervals (RIs) were: packed cell volume 38–59%; haemoglobin 105–154 g/l; RBC 2.3–3.3 106/µl; MCV 145.5–199.7 fl; MHC 38.3–55.6 pg; MCHC 232–313 g/l; WBC 2.7–12.6 103/µl; heterophils 1.2–10.2 103/µl; lymphocytes 0.8–4.3 103/µl; monocytes 0.0–0.6 103/µl; basophils 0.0–0.1 103/µl; eosinophils 0.0–0.2 103/µl. To the best of our knowledge, this is the first study in psittacines to use such a high number of birds and samples to establish haematological species-specific RIs, providing a valuable tool to professionals dealing with the health of African Grey parrots.
Molecular tests can contribute to improve the preoperative diagnosis of thyroid nodules. Tests available are expensive and not adapted to different populations.
This study aimed to compare the ...cyto-histological genetic profile and to evaluate the reliability of molecular tests using ultrasound-guided fine needle aspiration cytology (US-FNAC) in accurately diagnosing differentiated thyroid carcinomas (DTCs) and predicting biologic behavior of papillary thyroid carcinomas (PTCs).
The series included 259 patients with paired cyto-histological samples totaling 518 samples. The genetic alterations were analyzed via PCR/Sanger sequencing. The association with clinicopathologic features was evaluated in PTCs.
From the 259 patients included, histologies were 50 (19.3%) benign controls and 209 (80.7%) DTC cases, from which 182 were PTCs; cytologies were 5.8% non-diagnostic, 18.2% benign, 39% indeterminate, and 37.1% malignant. In histology, indeterminate nodules (
= 101) were 22.8% benign and 77.2% malignant. Mutation frequencies in cytology and histology specimens were, respectively,
: 3.7% vs. 7.9%;
: 19.5% vs. 25.1%; and
: 11% vs. 17.5%. The overall cyto-histological agreement of the genetic mutations was 94.9%, with Cohen's k = 0.67, and in indeterminate nodules agreement was 95.7%, k = 0.64. The identified mutations exhibited a discriminative ability in diagnosing DTC with a specificity of 100% for
and
, and of 94% for
, albeit with low sensitivity.
and
mutations were associated with aggressive clinicopathological features and tumor progression in PTCs (
< 0.001). The obtained good cyto-histological agreement suggests that molecular analysis via US-FNAC may anticipate the genetic profile and the behavior of thyroid tumors, confirming malignancy and contributing to referring patients to surgery.
Sambucus nigra L., popularly known as elderberry, is renowned for its amazing therapeutic properties, as well as its uses as a food source, in nutraceuticals, and in traditional medicine. This ...study’s aim was to investigate the effects of an elderberry extract (EE) on mice for 29 days, as well as the safety of the extract when used as a natural colorant. Twenty-four FVB/n female mice (n = 6) were randomly assigned to one of four groups: control, 12 mg/mL EE (EE12), 24 mg/mL EE (EE24), or 48 mg/mL EE (EE48). The predominant anthocyanins detected were cyanidin-3-O-sambubioside and cyanidin-3-O-glucoside. Food and drink intake were similar between groups, with the exception of EE48, who drank significantly less compared with the Control. Biochemical analysis of the liver showed that the changes observed in histological analysis had no pathological significance. The EE, at doses of 24 and 48 mg/mL, significantly reduced the oxidative DNA damage compared with the non-supplemented group. The S. nigra extract showed a favorable toxicological profile, affording it potential to be used in the food industry.
This work aimed to define a humane endpoint scoring system able to objectively identify signs of animal suffering in a rat model of type 2 diabetes. Sprague-Dawley male rats were divided into control ...and induced group. The induced animals drink a 10% fructose solution for 14 days. Then, received an administration of streptozotocin (40 mg/kg). Animals' body weight, water and food consumption were recorded weekly. To evaluate animal welfare, a score sheet with 14 parameters was employed. Blood glucose levels were measured at three time points. After seven weeks of initiating the protocol, the rats were euthanized. The induced animals showed weight loss, polyuria, polyphagia, and polydipsia. According to our humane endpoints table, changes in animal welfare became noticeable after the STZ administration. None of the animals hit the critical score limit (four). Data showed that the most effective parameters to assess welfare in this type 2 diabetes rat induction model were dehydration, grooming, posture, abdominal visualization, and stool appearance. The glycemia was significantly higher in the induced group when compared to the controls (
< 0.01). Induced animals' murinometric and nutritional parameters were significantly lower than the controls (
< 0.01). Our findings suggest that in this rat model of type 2 diabetes with STZ-induced following fructose consumption, our list of humane endpoints is suitable for monitoring the animals' welfare.
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