Listeria monocytogenes is a foodborne pathogen. A source of infection can be artisanal cheeses. Identification of the Listeria species is important for the protection of public health and the food ...industry. This study aimed to examine artisanal cheeses for the presence of L. monocytogenes and the effectiveness of the MALDI-TOF MS method in the identification of the L. monocytogenes isolates. A total of 370 samples of artisanal cheeses were examined. L. monocytogenes was found in 23 cheese samples (6.2%). The reliability of L. monocytogenes identification achieved by MALDI-TOF MS was varied, and the vast majority of the isolates (27/32) were identified only to the secure genus, probable species level. This study showed that (i) the occurrence of L. monocytogenes in the artisanal cheeses was at a higher level than that in the other EU countries, (ii) the standard of species identification of L. monocytogenes isolates from artisanal cheeses achieved by MALDI-TOF MS was not satisfactory and (iii) the presence of L. monocytogenes in artisanal cheeses remains a problem with regard to the food safety criterion and a potential public health risk.
The study focused on reconstruction of the extreme hydrological changes within the bog surrounding the lake Płotycze near Sobibór over the past 200 years. The site has been located within the reserve ...„Three Lakes”, in the Sobibór Landscape Park. The main aim was to determine the intensity of these changes and to determine the extent to which allogeneic and indigenous factors resulted in the functioning of peat bog. Analysis consisted of 0.60 m peat core extracted from the northwestern part of the bog, including the youngest periods in the history of the bog. This section, as follows directly from historical data, is also a carrier of data on intensive human impact on the area. As a research method testate amoebae analysis was used. These organisms belong to the dominant group of peat microorganisms. The results obtained enabled to determine two main paleohydrology phases in the last 200 years of the peat bog history. These phases have been correlated with historical data and with the results of previous studies carried out in the peat bog, i.e. reconstruction of water level changes based on data mapping using GIS tools and the study of plant communities.
Diabetes and Nephrotic syndrome (NS) promote plaque-related gingivitis and yeast-like fungal infections. The study assesses the impact of Candida spp. and general disease- or treatment-related ...factors on plaque-related gingivitis severity in children and adolescents with Nephrotic syndrome /diabetes.
Body mass index (BMI), BMI standard deviation score, and oral cavity (Plaque Index--PLI, Gingival Index--GI, mucosa status, presence and Candida enzymatic activity) were assessed in 96 patients (32 with NS: 30- immunosuppressive treatment, 35--type 1 diabetes, and 29 generally healthy), aged; 3-18 years. Laboratory included cholesterol and triglyceride measurements; in diabetic subjects- glycated haemoglobin, in NS: total protein, albumin, creatinine, haemoglobin, haematocrit, white cell count, urinary protein excretion. Medical records supplied information on disease duration and treatment. A statistical analysis was performed; Kendall Tau coefficient, chi-square test, t-test, and multiple regression analysis ( P < 0.05).
Candida spp. often occurred in healthy patients, but oral candidiasis was found only in the NS and diabetes groups (9.37% and 11.43%). Gingivitis occurred more frequently in patients with NS/diabetes. Gingivitis severity was correlated with PLI, age, and yeast enzyme activity in NS--to with immunosuppressive treatment with >1 drug, drug doses, treatment duration, lipid disorders, and BMI; in diabetes, with blood glucose and glycated haemoglobin >8%.
Poor hygiene control is the main cause of gingivitis. Gingivitis severity is most likely related to age, lipid disorders and increase in body mass. Candida spp., in uncompensated diabetes and in those using immunosuppressive treatment, might intensify plaque-related gingivitis.
This article summarizes the evidence for management of attention-deficit/hyperactivity disorder (ADHD) using chronic aerobic physical activity (PA). Known studies comparing chronic aerobic PA to at ...least one control group are listed; uncontrolled studies and studies of non-aerobic PA are not considered. Key challenges to conducting chronic PA studies with children and youth with ADHD are summarized. After condensing information from widely varying studies, measures, and research designs, conclusions are stated in broad brush stroke terms. Preliminary evidence supports PA as beneficial for ADHD symptoms, executive function, and motor abilities. Social, emotional, and behavioral outcomes also may benefit. The preliminary state of the evidence supports PA as an adjunctive treatment for ADHD at this time, but the body and sophistication of the research to date is insufficient at present to support PA as a stand-alone treatment. Critical directions for future research are discussed.
artykuł w: Annales Universitatis Mariae Curie-Skłodowska, Sectio B, Geographia, Geologia, Mineralogia et Petrographia Vol. 70 (2015), 2, s. 45-61
streszcz. ang.
tyt. równol. w jęz. ang.: The use of ...testate amoebae analysis to reconstruct hydrological changes of Płotycze near Sobibor
Article in: Annales Universitatis Mariae Curie-Skłodowska, Sectio B, Geographia, Geologia, Mineralogia et Petrovol. 70 (2015), 2, p. 45-61
tit. equilol. In English. EnglishUżycie amebae testate analyzes reconstruct hydrological changes of Płotycze near Sobibor
artykuł w: Annales Universitatis Mariae Curie-Skłodowska, Sectio B, Geographia, Geologia, Mineralogia et Petrographia Vol. 70 (2015), 2, s. 45-61
tyt. równol. w jęz. ang.: The use of testate amoebae analysis to reconstruct hydrological changes of Płotycze near Sobibor
Pseudoachondroplasia (PSACH) is one of the most frequent skeletal dysplasias and results exclusively from mutations in cartilage oligomeric matrix protein (COMP). COMP is a large pentameric ...glycoprotein found in cartilage, tendon and ligament. It consists of an amino-terminal oligomerisation domain, followed by 4 EGF-like repeats, 8 TSP type 3 repeats and a globular C-terminal domain (CTD). Most of the mutations found to date cluster in the type 3 repeats region (85%) and the cell-matrix pathology of these mutations has been studied in detail. It has been shown that abnormal COMP protein is retained in the rER of chondrocytes together with other extracellular matrix (ECM) proteins postulated to interact with COMP, and eventually these form large lamellar inclusion bodies, which finally result in cell death and a reduction in the number of viable cells in the tissue. Pathomolecular mechanism of PSACH resulting from CTD mutations (15%) remains largely unknown due to the difficulty in obtaining suitable pathological material from the affected patients. When patients' material is not available the study of animal models of human diseases allows insight into the mechanism of the disease that otherwise would not be possible. A targeting construct containing a mutated comp gene was generated using a Female Mouse (129S6/SvEvTac) PAC Library. The DNA was integrated into the mouse genome by gene targeting in R1 ES cells (129Sv) and the recombinant cells were microinjected into C57BL blastocysts to yield high quality chimeric mice. After successful germline transmission F2 mice were bred together, yielding offspring in a Mendelian distribution. The generated knock-in mice were analysed by weight monitoring, bone measurements, histology, immunohistochemistry and electron microscopy. T583M COMP mouse is the first mouse model of pseudoachondroplasia and mutant animals grow significantly slower than their littermates. Mice homozygous for the mutation are normal at birth but with age develop a mild pelvic malformation and by 9 weeks have significantly shorter long bones with normal skull measurements. In the cartilage growth plate of mice homozygous for the mutation the chondrocytes are sparser and more disorganised, whilst COMP is reduced in the territorial matrix. These data demonstrate that mice carrying a T583M mutation have a phenotype similar to mild pseudoachondroplasia and they can be used to further investigate the pathomolecular mechanisms of pseudoachondroplasia resulting from a C-terminal COMP mutation.
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