Supermassive black hole binaries (SMBHBs) at sub-parsec separations should be common in galactic nuclei, as a result of frequent galaxy mergers. Hydrodynamical simulations of circum-binary discs ...predict strong periodic modulation of the mass accretion rate on time-scales comparable to the orbital period of the binary. As a result, SMBHBs may be recognized by the periodic modulation of their brightness. We conducted a statistical search for periodic variability in a sample of 35 383 spectroscopically confirmed quasars in the photometric data base of the Palomar Transient Factory (PTF). We analysed Lomb–Scargle periodograms and assessed the significance of our findings by modelling each individual quasar's variability as a damped random walk (DRW). We identified 50 quasars with significant periodicity beyond the DRW model, typically with short periods of a few hundred days. We find 33 of these to remain significant after a re-analysis of their periodograms including additional optical data from the intermediate-PTF and the Catalina Real-Time Transient Survey. Assuming that the observed periods correspond to the redshifted orbital periods of SMBHBs, we conclude that our findings are consistent with a population of unequal-mass SMBHBs, with a typical mass ratio as low as q ≡ M
2/M
1 ≈ 0.01.
We present 1201 galaxies at 0.05 < z < 0.45 that host tidal features in the first ∼200 deg2 of imaging from the Hyper Suprime-Cam Subaru Strategic Program (HSC-SSP). We select these galaxies from a ...sample of 21,208 galaxies with spectroscopic redshifts drawn from the Sloan Digital Sky Survey (SDSS) spectroscopic campaigns. Of these galaxies, we identify 214 shell systems and 987 stream systems. For 575 of these systems, we are additionally able to measure the (g−i) colors of the tidal features. We find evidence for star formation in a subset of the streams, with the exception of streams around massive ellipticals, and find that stream host galaxies span the full range of stellar masses in our sample. Galaxies that host shells are predominantly red and massive: we find that observable shells form more frequently around ellipticals than around disk galaxies of the same stellar mass. Although the majority of the shells in our sample are consistent with being formed by minor mergers, 15% 4.4% of shell host galaxies have (g−i) colors as red as their host galaxy, consistent with being formed by major mergers. These "red shell" galaxies are preferentially aligned with the major axis of the host galaxy, as previously predicted from simulations. We suggest that although the bulk of the observable shell population originates from fairly minor mergers, which preferentially form shells that are not aligned with the major axis of the galaxy, major mergers produce a significant number of observable shells.
In a companion paper by Koposov et al., RR Lyrae from Gaia Data Release 2 are used to demonstrate that stars in the Orphan stream have velocity vectors significantly misaligned with the stream track, ...suggesting that it has received a large gravitational perturbation from a satellite of the Milky Way. We argue that such a mismatch cannot arise due to any realistic static Milky Way potential and then explore the perturbative effects of the Large Magellanic Cloud (LMC). We find that the LMC can produce precisely, the observed motion-track mismatch and we therefore use the Orphan stream to measure the mass of the Cloud. We simultaneously fit the Milky Way and LMC potentials and infer that a total LMC mass of |$1.38^{+0.27}_{-0.24} \times 10^{11}\, \rm {M_\odot}$| is required to bend the Orphan stream, showing for the first time that the LMC has a large and measurable effect on structures orbiting the Milky Way. This has far-reaching consequences for any technique which assumes that tracers are orbiting a static Milky Way. Furthermore, we measure the Milky Way mass within 50 kpc to be |$3.80^{+0.14}_{-0.11}\times 10^{11} \, \mathrm{M}_\odot$|. Finally, we use these results to predict that, due to the reflex motion of the Milky Way in response to the LMC, the outskirts of the Milky Way’s stellar halo should exhibit a bulk, upwards motion.
Abstract
We use astrometry, broad-band photometry, and variability information from the Data Release 2 of ESA’s Gaia mission (GDR2) to identify members of the Orphan Stream (OS) across the whole sky. ...The stream is traced above and below the celestial equator and in both Galactic hemispheres, thus increasing its visible length to ∼210° equivalent to ∼150 kpc in physical extent. Taking advantage of the large number of RR Lyrae stars in the OS, we extract accurate distances and proper motions across the entire stretch of the tidal debris studied. As delineated by the GDR2 RR Lyrae, the stream exhibits two prominent twists in its shape on the sky which are accompanied by changes in the tangential motion. We complement the RR Lyrae maps with those created using GDR2 Red Giants and the DECam Legacy Survey Main Sequence Turn-Off stars. The behaviour of the OS track on the sky is consistent across all three tracers employed. We detect a strong non-zero motion in the across-stream direction for a substantial portion of the stream. Such a misalignment between the debris track and the streaming velocity cannot be reproduced in a static gravitational potential and signals an interaction with a massive perturber.
Graham et al. discovered a supermassive black hole binary (SMBHB) candidate and identified the detected 5.2 yr period of the optical variability as the orbital period of the binary. Hydrodynamical ...simulations predict multiple periodic components for the variability of SMBHBs, thus raising the possibility that the true period of the binary is different from 5.2 yr. We analyse the periodogram of PG1302 and find no compelling evidence for additional peaks. We also point out that, despite the 5.2 yr peak being significant if a single source is considered, further analysis is required to account for the fact that PG1302 was selected among a large sample of 247 000 quasars. We derive upper limits on any additional periodic modulations in the available data, by modelling the light curve as the sum of stochastic noise and the known 5.2 yr periodic component, and injecting additional sinusoidal signals. We find that, with the current data, we would be able to detect with high significance (false alarm probability <1 per cent) secondary periodic terms, with periods in the range predicted by the simulations, if the amplitude of the variability was at least ∼0.06 mag (compared to 0.14 mag for the main sinusoid). A three-year follow-up monitoring campaign with weekly observations can increase the sensitivity for detecting secondary peaks by ≈50 per cent, and would allow a more robust test of predictions from hydrodynamical simulations.
Altered intestinal dendritic cell (DC) function underlies dysregulated T-cell responses to bacteria in Crohn's disease (CD) but it is unclear whether composition of the intestinal microbiota impacts ...local DC function. We assessed the relationship between DC function with disease activity and intestinal microbiota in patients with CD.
Surface expression of Toll-like receptor (TLR)-2, TLR-4, and spontaneous intracellular interleukin (IL)-10, IL-12p40, IL-6 production by freshly isolated DC were analyzed by multicolor flow cytometry of cells extracted from rectal tissue of 10 controls and 28 CD patients. Myeloid DC were identified as CD11c(+) HLA-DR(+lin-/dim) cells (lin = anti-CD3, CD14, CD16, CD19, CD34). Intestinal microbiota were analyzed by fluorescent in situ hybridization of fecal samples with oligonucleotide probes targeting 16S rRNA of bifidobacteria, bacteroides-prevotella, C. coccoides-E. rectale, and Faecalibacterium prausnitzii.
DC from CD produced higher amounts of IL-12p40 and IL-6 than control DC. IL-6(+) DC were associated with the CD Activity Index (r = 0.425; P = 0.024) and serum C-reactive protein (CRP) (r = 0.643; P = 0.004). DC expression of TLR-4 correlated with disease activity. IL-12p40(+) DC correlated with ratio of bacteroides: bifidobacteria (r = 0.535, P = 0.003). IL-10(+) DC correlated with bifidobacteria, and IL-6(+) DC correlated negatively with F. prausnitzii (r = -0.50; P = 0.008). The amount of TLR-4 on DC correlated negatively with the concentration of F. prausnitzii.
IL-6 production by intestinal DC is increased in CD and correlates with disease activity and CRP. Bacterially driven local IL-6 production by intestinal DC may overcome regulatory activity, resulting in unopposed effector function and tissue damage. Intestinal DC function may be influenced by the composition of the commensal microbiota.
Tidally disrupted globular clusters are transformed into thin, dynamically cold streams of stars that are extremely valuable tracers of the large- and small-scale distribution of mass in the Galaxy. ...Using data from the Gaia second data release combined with Pan-STARRS photometry, we present a sample of highly probable members of the longest cold stream in the Milky Way, GD-1. The resulting map of GD-1: (1) extends the apparent length of the stream by 20°, (2) reveals plausible locations for the progenitor, (3) detects high-contrast gaps along the stream, and (4) indicates the existence of stream members perturbed off the main stream track. These discoveries are only possible because of the exquisite astrometry from Gaia, which permits a clean separation of the stream from Milky Way stars. The additional length and a proper treatment of the progenitor will aid in dynamical modeling of GD-1 for mapping the large-scale dark matter distribution. The complex morphology of the stream points to a turbulent history; detailed phase-space properties of the perturbed stream members could potentially constrain dark matter substructure in the Milky Way.
Neuronal assemblies within the nervous system produce electrical activity that can be recorded in terms of action potential patterns. Such patterns provide a sensitive endpoint to detect effects of a ...variety of chemical and physical perturbations. They are a function of synaptic changes and do not necessarily involve structural alterations. In vitro neuronal networks (NNs) grown on micro-electrode arrays (MEAs) respond to neuroactive substances as well as the in vivo brain. As such, they constitute a valuable tool for investigating changes in the electrophysiological activity of the neurons in response to chemical exposures. However, the reproducibility of NN responses to chemical exposure has not been systematically documented. To this purpose six independent laboratories (in Europe and in USA) evaluated the response to the same pharmacological compounds (Fluoxetine, Muscimol, and Verapamil) in primary neuronal cultures. Common standardization principles and acceptance criteria for the quality of the cultures have been established to compare the obtained results. These studies involved more than 100 experiments before the final conclusions have been drawn that MEA technology has a potential for standard in vitro neurotoxicity/neuropharmacology evaluation. The obtained results show good intra- and inter-laboratory reproducibility of the responses. The consistent inhibitory effects of the compounds were observed in all the laboratories with the 50% Inhibiting Concentrations (IC(50)s) ranging from: (mean ± SEM, in μM) 1.53 ± 0.17 to 5.4 ± 0.7 (n = 35) for Fluoxetine, 0.16 ± 0.03 to 0.38 ± 0.16 μM (n = 35) for Muscimol, and 2.68 ± 0.32 to 5.23 ± 1.7 (n = 32) for Verapamil. The outcome of this study indicates that the MEA approach is a robust tool leading to reproducible results. The future direction will be to extend the set of testing compounds and to propose the MEA approach as a standard screen for identification and prioritization of chemicals with neurotoxicity potential.
Tetrameric peptide-MHC class I complexes ("tetramers") are proving invaluable as reagents for characterizing immune responses involving CTLs. However, because the TCR can exhibit a degree of ...promiscuity for binding peptide-MHC class I ligands, there is potential for cross-reactivity. Recent reports showing that the TCR/peptide-MHC interaction is dramatically dependent upon temperature led us to investigate the effects of incubation temperature on tetramer staining. We find that tetramers rapidly stain CTLs with high intensity at 37 degrees C. We examine the fine specificity of tetramer staining using a well-characterized set of natural epitope variants. Peptide variants that elicit little or no functional cellular response from CTLs can stain these cells at 4 degrees C but not at 37 degrees C when incorporated into tetramers. These results suggest that some studies reporting tetramer incubations at 4 degrees C could detect cross-reactive populations of CTLs with minimal avidity for the tetramer peptide, especially in the tetramer-low population. For identifying specific CTLs among polyclonal cell populations such as PBLs, incubation with tetramers at 37 degrees C improves the staining intensity of specific CTLs, resulting in improved separation of tetramer-high CD8+ cells. Confocal microscopy reveals that tetramers incubated at 37 degrees C can be rapidly internalized by specific CTLs into vesicles that overlap with the early endocytic compartment. This TCR-specific internalization suggests that coupling of tetramers or analogues with toxins, which are activated only after receptor internalization, may create immunotoxins capable of killing CTLs of single specificities.
Aims. Several kinematic and chemical substructures have been recently found amongst Milky Way halo stars with retrograde motions. It is currently unclear how these various structures are related to ...each other. This Letter aims to shed light on this issue. Methods. We explore the retrograde halo with an augmented version of the Gaia DR2 RVS sample, extended with data from three large spectroscopic surveys, namely RAVE, APOGEE, and LAMOST. In this dataset, we identify several structures using the HDBSCAN clustering algorithm. We discuss their properties and possible links using all the available chemical and dynamical information. Results. In concordance with previous work, we find that stars with Fe/H < −1 have more retrograde motions than those with Fe/H > −1. The retrograde halo contains a mixture of debris from objects like Gaia-Enceladus, Sequoia, and even the chemically defined thick disc. We find that the Sequoia has a smaller range in orbital energies than previously suggested and is confined to high energy. Sequoia could be a small galaxy in itself, but since it overlaps both in integrals-of-motion space and chemical abundance space with the less bound debris of Gaia-Enceladus, its nature cannot yet be fully settled. In the low-energy part of the halo, we find evidence for at least one more distinct structure: Thamnos. Stars in Thamnos are on low-inclination, mildly eccentric retrograde orbits, moving at vϕ ≈ −150 km s−1, and are chemically distinct from the other structures. Conclusions. Even with the excellent Gaia DR2 data, piecing together all the fragments found in the retrograde halo remains challenging. At this point, we are very much in need of large datasets with high-quality high-resolution spectra and tailored high-resolution hydrodynamical simulations of galaxy mergers.
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