The inhibitory effects of isoliensinine (IL), a bisbenzylisoquinoline alkaloid extracted from the seed embryo of the traditional chinese medicinal herb Nelumbo nucifera Gaertn, on the proliferation ...of porcine coronary arterial smooth muscle cells (CASMCs) induced by angiotensin II(Ang II) and its mechanisms of action were investigated. Counting cultured cell number, MTT assay, immunohistochemical method and Western blot were adopted. Ang II 0.1 μmol l
− 1
significantly evoked CASMC proliferation by 42%, which could be dose-dependently inhibited by IL 0.01-3 μmol l
− 1
and the percentage of inhibition of IL 0.1 μmol l
− 1
was 25%. Irbesartan (Irb) 0.1 μmol l
− 1
inhibited CASMC proliferation by 22%. IL or Irb 0.1 μmol l
− 1
decreased Ang II-induced overexpression of Platelet-derived growth factor (PDGF)-β and basic fibroblast growth factor (bFGF), respectively. Both of them also declined c-fos, c-myc and hsp70 overexpression, respectively. At the same concentration, the inhibitory effects of IL on PDGF-β were even stronger than those of Irb (P < 0.05). In summary, the data showed that IL possesses an anti-proliferative effect, which is related to the decrease of the overexpression of growth factors PDGF-β, bFGF, proto-oncogene c-fos, c-myc and hsp70.
To evaluate the effects of artemisinin on proliferation, cell cycle and apoptosis of gallbladder cancer cells.
Gallbladder carcinoma cell lines(GBC-SD and NOZ)were cultured in vitro. The effects of ...artemisinin in different concentration on proliferation of the two cell lines in vitro were examined using MTT assay. The cell cycle distribution of GBC-SD and NOZ cells 24 h after treatments with artemisinin(20 μmol/L) were examined using flow cytometry. The apoptosis of GBC-SD and NOZ cells 24 h after treatments with artemisinin (20 μmol/L) were examined using Annexin V/PI staining.The expressions of p-ERK1/2, CDK4, cyclin D1, p16, cytochrome C and caspase-3 were examined by Western blot assay. t-test and one way ANOVA were used to evaluate the differences between two groups and more than two groups, respectively.
The cell proliferation was significantly inhibited by artemisinin, the IC50 of artemisinin against GBC-SD and NOZ cells were 14.05 μmol/L and 12.42 μmol/L, respectively.Artemisinin induced cycle arrest,
The trace mineral selenium inhibits cancer development in a variety of experimental animal models. We used an in vivo canine model to evaluate the effects of dietary selenium supplementation on DNA ...damage in prostate tissue and on apoptosis in prostate epithelial cells. Sexually intact elderly male beagle dogs were randomly assigned to receive an unsupplemented diet (control group) or diets that were supplemented with selenium (treatment group), either as selenomethionine or as high-selenium yeast at 3 μg/kg or 6 μg/kg body weight per day for 7 months. The extent of DNA damage in prostate cells and in peripheral blood lymphocytes, as determined by the alkaline comet assay, was lower among the selenium-supplemented dogs than among the control dogs (prostate P<.001; peripheral blood lymphocytes P = .003; analysis of variance) but was not associated with the activity of the antioxidant enzyme glutathione peroxidase in plasma. The median number of terminal deoxynucleotidyl transferase–mediated dUTP nick end-labeling-positive (i.e., apoptotic) prostate epithelial cells was 3.7 (interquartile range = 1.1–7.6) for the selenium-supplemented dogs and 1.7 (interquartile range = 0.2–2.8) for the control dogs ( P = .04, Mann–Whitney U test). These data suggest that dietary selenium supplementation decreases DNA damage and increases epithelial cell apoptosis within the aging canine prostate.
Mangrove wetlands have been rapidly diminishing because of human pressures worldwide. The Guangdong Province in South China, which has the largest area of mangrove wetlands in the nation, is under ...severe threat as a result of rapid urbanization and economic development. In this paper, comparisons were made between optical Landsat TM images and Radarsat fine-mode images for estimating wetland biomass. Regression and analytical models were used to establish the relationships between remote sensing data and wetland biomass. The optimal parameter values for the analytical model were determined using genetic algorithms. Experiments indicate that the models using Radarsat fine-mode images have significant accuracy improvement in terms of Root Mean-Square Error (RMSE) whereas the use of the single Normalized Difference Vegetation Index (NDVI) may produce serious errors in biomass estimation. The Radarsat images can obtain more accurate trunk information about mangrove forests because of higher resolution and side-looking geometry. The use of genetic algorithms can help to decompose backscatter into vegetation and soil backscattering, which is very useful for ecological modelling.
Thyroid associated ophthalmopathy is a complex autoimmune disease characterized by inflammation of the orbital tissue, hyaluronan deposition and tissue remodeling due to a skewed production of ...preadipocytic fibroblasts and increased adipogenesis in the late stage. Its progression is strongly related to the functional status of thyroid. So far, there is no consensus on staging, grading and treatment worldwide owning to the complexity of its clinical manifestations and therapies. However, it is of great importance to accurately judge its status and make individualized therapeutic regimen. Therefore, we propose how to standardize the TAO diagnosis and treatment.
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Telomerase reverse transcriptase (hTERT) is the key determinant of telomerase activity and plays a crucial role in cellular immortalization and oncogenesis. It will be a promising target for cancer ...gene therapy. We constructed a novel replicative adenovirus CNHK300 in which hTERT promoter with three extra E-boxes downstream of the promoter was introduced and used to regulate adenoviral E1a gene, and studied its properties of selective replication in cancer cells and antitumoral activity.
Luciferase assay was used to detect hTERT promoter activity. The selective replication of CNHK300 in cancer cells was investigated by E1a Western blot and green fluorescent protein (GFP) reporter gene assay. The antitumoral activity of CNHK300 and its toxicity were measured on animal models.
Luciferase assay showed that introducing extra E-boxes downstream of hTERT promoter is beneficial to decreasing the promoter activity in normal cells without affecting its strong activity in cancer cells. Experiments in vitro and in vivo demonstrated that CNHK300 can selectively target to hTERT-positive cancer cells and replicate in them, resulting in oncolytic or antitumoral effect. CNHK300 is superior to ONYX-015 in terms of selective replication and oncolytic or antitumoral effect. The toxicity assay showed no signs of toxicity to liver cells even at the higher dosage of CNHK300 in vivo.
The hTERT promoter-controlled, replication-competent adenovirus CNHK300 is a promising system for targeted cancer gene therapy.
A total of 86 representative samples of diesel oil were selected to determine the solidifying point. These samples covered a wide range and the concentration is more evenly distributed. The near ...infrared spectroscopy (NIR) method to determine the diesel solidifying point was successfully designed. Multiple linear regression method was chosen to establish the calibration model. The regression coefficients, partial regression sum of squares, partial correlation coefficients, the total estimated standard deviation, correlation coefficient, residual sum of squares, and other model parameters were calculated. The repeatability of the NIR method is better than the standard method. It can be seen that this NIR method to determine the diesel solidifying is feasible and repeatable.
Due to the limits of size and weight of probes, analysis and design of the low-energy escaping orbit play a significant role in saving the energy in space exploration. In this paper, we research the ...mechanism of the evolution of the probe orbital energy and develop a design method for the low-energy escaping orbits in the Earth–Moon system. A dynamic model accounting for the Sun–Earth–Moon-probe elliptical four-body problem is presented by considering Moon’s eccentricity and Sun’s direct gravitational influence. Considering the influences from phase of Earth, Moon and Sun, the equations of the probe orbital energy and its variation are derived and theoretical analysis is implemented based on corresponding energy expressions. Then, the Poincaré mapping technique is utilized to search two types of low-energy families of escaping orbits and the results of numerical simulation confirm the theoretical predictions. The dynamic model proposed in this paper is more accurate and has practical value comparing with the circular restricted three-body problem, and the escaping strategy can save over 25 % of energy relative to the hyperbolic escaping.
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