Summary： Recently, suppressor of cytokine signaling-3 （SOCS3） has been shown to be an inducible endogenous negative regulator of Janus kinase/signal transducers and activators of transcription ...（JAK/STAT） pathway which is relevant in inflammatory response, while its functions in acute liver failure and HBV-induced acute-on-chronic liver failure （HBV-ACLF） have not been fully elucidated. In this study, we explored the role of SOCS3 in the development of mouse hepatitis virus strain 3 （MHV-3）-induced acute liver failure and its expression in liver and peripheral blood mononuclear cells （PBMCs） of patients with HBV-ACLF. Inflammation-related gene expression was detected by real-time PCR, immtmohistochemistry and Western blotting. The correlation between SOCS3 level and liver injury was studied. Our results showed that the SOCS3 expression was significantly elevated in both the liver tissue and PBMCs from patients with HBV-ACLF compared to mild chronic hepatitis B （CHB）. Moreover, a time course study showed that SOCS3 level was increased remarkably in the liver of BALB/cJ mice at 72 h post-infection. Pro-inflammatory cytokines, interleukin （IL）-1 β, IL-6, and tumor necrosis factor （TNF）-α, were also increased significantly at 72 h post-infection. There was a close correlation between hepatic SOCS3 level and IL-6, and the severity of liver injury defined by alanine aminotransferase （ALT） and aspartate aminotransferase （AST） levels, respectively. These data suggested that SOCS3 may play a pivotal role in the pathogenesis of MHV-3-induced acute liver failure and HBV-ACLF.
To develop a system for quick screening of efficient siRNA targeted HBx mRNA.
Using recombination DNA technique, the fusion expression plasmid of HBx and EGFP was constructed, and siRNA expression ...cassettes (SECs) containing U6+1, H1 or tRNA(Val )promoter were prepared via one-step overlapping extension PCR. By co-transfection with recombinant plasmid and SECs into AD293 cell, the inhibition effects on the transient expression of HBx-EGFP fusion protein were analyzed by FACS and semi-quantitated RT-PCR analysis.
(1)HBx-EGFP fusion protein expression plasmid pHBx-EGFP was constructed successfully, which expressed green fluorescence in cell mainly located at plasma or the periphery of nucleus in granules. (2) Co-transfection with recombinant plasmid and SECs into AD293 cells resulted in inhibition of HBx-EGFP expression. SEC-siHBx388 showed significant inhibition effect on HBx-EGFP expression compared with SEC-siHBx271, indicating that siHBx388 is effective siRNA site and could be screened out with our screenin
The thermal conductivity of graphene nanoribbons (layer from 1 to 8 atomic planes) is investigated by using the nonequilibrium molecular dynamics method. We present that the room-temperature thermal ...conductivity decays monotonically with the number of the layers in few-layer graphene. The superiority of zigzag graphene in thermal conductivity is only available in high temperature region and disappears in multi-layer case. It is explained that the phonon spectral shrink in high frequency induces the change of thermal conductivity. It is also reported that single-layer graphene has better ballistic transport property than the multi-layer graphene.
We investigate the thermal conductivity of four types of deformed carbon nanotubes by using the nonequilibrium molecular dynamics method. It is reported that various deformations have different ...influence on the thermal properties of carbon nanotubes. For the bending carbon nanotubes, the thermal conductivity is independent on the bending angle. However, the thermal conductivity increases lightly with XY-distortion and decreases rapidly with Z-distortion. The thermal conductivity does not change with the screw ratio before the breaking of carbon nanotubes but decreases sharply after the critical screw ratio.
Phys. Rev. D 97, 052008 (2018) Using a data sample of $448.1\times10^6$ $\psi(3686)$ events collected with
the BESIII detector operating at the BEPCII, we perform search for the hadronic
transition ...$h_c\rightarrow\pi^+\pi^-J/\psi$ via
$\psi(3686)\rightarrow\pi^0h_c$. No signals of the transition are observed, and
the upper limit on the product branching fraction
$\mathcal{B}(\psi(3686)\rightarrow\pi^0h_c)\mathcal{B}(h_c\rightarrow\pi^+\pi^-J/\psi)$
at the 90% confidence level is determined to be $2.0\times10^{-6}$. This is the
most stringent upper limit to date.
Physics Letters B 774, 252 (2017) Using a data set of 2.93 fb$^{-1}$ taken at a center-of-mass energy
$\sqrt{s}$ = 3.773 GeV with the BESIII detector at the BEPCII collider, we
perform a search for ...an extra U(1) gauge boson, also denoted as a dark photon.
We examine the initial state radiation reactions $e^+e^-\rightarrow
e^+e^-\gamma_{\rm ISR}$ and $e^+e^-\rightarrow \mu^+\mu^-\gamma_{\rm ISR}$ for
this search, where the dark photon would appear as an enhancement in the
invariant mass distribution of the leptonic pairs. We observe no obvious
enhancement in the mass range between 1.5 and 3.4 GeV/$c^{2}$ and set a 90%
confidence level upper limit on the mixing strength of the dark photon and the
Standard Model photon. We obtain a competitive limit in the tested mass range.
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