To investigate the application value of Ki67 and serum CA125 in diagnosing the deep myometrial invasion of endometrial adenocarcinoma.
This study retrospectively analyzed 80 patients with endometrial ...adenocarcinoma, who underwent procedure from January 2018 to June 2021 at Senior Department of Obstetrics & Gynecology, the Seventh Medical Center of PLA General Hospital assigned to the Fourth Medical Center. The general clinical data, serum CA125 and Ki67 levels were compared between the superficial muscular infiltration group and the deep myometrial invasion group. We investigated the application value of Ki67 and serum CA125 in diagnosing the deep myometrial invasion of endometrial adenocarcinoma by the ROC curve.
80 patients were retrospectively analyzed, and 53 cases were superficial muscular infiltration, 27 cases were deep myometrial invasion. There was significant difference in age, tumor diameter, lymph node metastasis, Ki67, serum CA125, p53 status, serum CA125 and Ki67 levels between the two groups (p < 0.05). As high as 35% of Ki67 was the optimal cutoff value for predicting DMI in endometrial adenocarcinoma, and the area under ROC curve was 0.691, the sensitivity and specificity of diagnosis were 88.9% and 56.6%. As high as 43.645 U/ml of serum CA125 was the optimal cutoff value for predicting DMI in endometrial adenocarcinoma, and the area under ROC curve was 0.668, the sensitivity and specificity of diagnosis were 40.7% and 92.5%. After combined detection of both, the area under ROC curve was 0.719, and its sensitivity and specificity of diagnosis were 96.3% and 43.4%.
Serum CA125 and Ki67 may be used to evaluate DMI in patients with endometrial adenocarcinoma, and the diagnostic value of combination is higher, which provide reference for clinical treatment.
Flexible tactile sensors are considered as an effective way to realize the sense of touch, which can perform the synchronized interactions with surrounding environment. Here, the utilization of ...bionic microstructures on natural lotus leaves is demonstrated to design and fabricate new‐type of high‐performance flexible capacitive tactile sensors. Taking advantage of unique surface micropattern of lotus leave as the template for electrodes and using polystyrene microspheres as the dielectric layer, the proposed devices present stable and high sensing performance, such as high sensitivity (0.815 kPa−1), wide dynamic response range (from 0 to 50 N), and fast response time (≈38 ms). In addition, the flexible capacitive sensor is not only applicable to pressure (touch of a single hair), but also to bending and stretching forces. The results indicate that the proposed capacitive tactile sensor is a promising candidate for the future applications in electronic skins, wearable robotics, and biomedical devices.
The utilization of bionic microstructures on natural lotus leaves are demonstrated to design and fabricate a new‐type flexible capacitive tactile sensor, the proposed device exhibit high sensitivity (0.815 kPa−1), wide dynamic response range (from 0 to 50 N), and fast response time (≈38 ms), which is perfectly applicable to pressure, bending, and stretching forces.
Objective
To explore the application of neutrophils to lymphocytes ratio (NLR), platelet to lymphocyte ratio (PLR) and monocyte to lymphocyte ratio (MLR) in the differential diagnosis of benign ...uterine diseases and endometrial malignant tumors.
Methods
80 patients with endometrial malignant tumor diagnosed in our hospital from January 2019 to December 2022 were selected as the study group, and 74 patients with benign uterine diseases confirmed by pathology in our hospital during the same period were randomly selected as the control group. The differences of NLR, PLR and MLR in the peripheral blood of patients in each group were compared, and the value of individual indicators in the diagnosis of endometrial malignant tumor was evaluated using the Receiver Operating Characteristic (ROC) curve.
Results
In peripheral blood, the NLR, PLR and MLR value in patients with endometrial cancer (EC) were significantly higher than those in patients with benign uterine diseases (P < 0.05). The area under the curve (AUC) of NLR, PLR, MLR in peripheral blood were 0.777, 0. 675 and 0.698. The best cutoff values were 2.02, 132.06 and 0.166. The sensitivity and specificity were 62.5% and 79.7%, 62.5% and 63.5%, 81 3% and 47.3%. The combination of these three indicators can significantly improved the diagnostic efficiency in endometrial cancer (AUC = 0.780), and the sensitivity and specificity were 60% and 83.8%.
Conclusions
In peripheral blood, NLR, PLR and MLR have certain diagnostic value in the differential diagnosis of endometrial cancer. When NLR, PLR and MLR are elevated, we should be alert to the occurrence of endometrial malignant tumors, and the combined diagnostic efficiency is high.
Currently, it is still a significant challenge to simultaneously boost various reactions by one electrocatalyst with high activity, excellent durability, as well as low cost. Herein, hybrid ...trifunctional electrocatalysts are explored via a facile one‐pot strategy toward an efficient oxygen reduction reaction (ORR), oxygen evolution reaction (OER), and hydrogen evolution reaction (HER). The catalysts are rationally designed to be composed by FeCo nanoparticles encapsuled in graphitic carbon films, Co2P nanoparticles, and N,P‐codoped carbon nanofiber networks. The FeCo nanoparticles and the synergistic effect from Co2P and FeCo nanoparticles make the dominant contributions to the ORR, OER, and HER activities, respectively. Their bifunctional activity parameter (∆E) for ORR and OER is low to 0.77 V, which is much smaller than those of most nonprecious metal catalysts ever reported, and comparable with state‐of‐the‐art Pt/C and RuO2 (0.78 V). Accordingly, the as‐assembled Zn–air battery exhibits a high power density of 154 mW cm−2 with a low charge–discharge voltage gap of 0.83 V (at 10 mA cm−2) and excellent stability. The as‐constructed overall water‐splitting cell achieves a current density of 10 mA cm−2 (at 1.68 V), which is comparable to the best reported trifunctional catalysts.
Trifunctional electrocatalysts based on FeCo/Co2P hybrid nanoparticles are reported, which have excellent activities and stabilities toward the oxygen reduction reaction, oxygen evolution reaction, and hydrogen evolution reaction. The as‐assembled Zn–air battery exhibits charge–discharge voltage gap of 0.83 V at 10 mA cm−2, and an as‐constructed overall water‐splitting cell achieves a current density of 10 mA cm−2 at 1.68 V.
Domestic trade plays a key role in China’s rapid economic progress. However, the increased domestic trade causes significant variations in carbon emission transfer among provinces. This study adopted ...the multi-region input-output (MRIO) model and social network analysis (SNA) to estimate the carbon emission transfer. Furthermore, the carbon emission transfer network characteristics among 30 provinces and 27 sectors were analyzed by using interprovincial input-output tables for 2007, 2010, and 2012. The results showed that (1) Large differences exist in carbon emission transfer flow and its network characteristics between provinces. (2) The three industrial sectors of metal smelting and pressing sector, power, heat production, and supply sector, petroleum processing, coking, and nuclear fuel processing sector have high carbon emission transfer and pose a strong influence on the carbon emission transfer network. (3) Provinces of the eastern region have a “bidirectional spillover” role, while those of the western region have a mediating role as an “agent.” Provinces of the central region have a “main inflow” role. Finally, useful policy implications and suggestions of this study are summarized.
Receptor mediated transcytosis (RMT) is a common mechanism used for nanotherapeutics to traverse the blood–brain barrier (BBB). However, the transcytosis of ligand modified nanoparticles via RMT is ...likely to be trapped within brain capillary endothelial cells due to the high binding affinity of ligand with receptors, which greatly reduces the amount of nanoparticles across BBB. Here, P‐aminophenyl‐α‐D‐mannopyranoside (MAN) decorated doxorubicin‐loaded dendrigraft poly‐l‐lysine with acid‐cleavable transferrin (Tf) coating outside (DD‐MCT) is proposed. The DD‐MCT is engineered to specifically recognize the Tf receptor (TfR) on the luminal side of BBB endothelium. Then the DD‐MCT undergoes an acid‐responsive cleavage of Tf, leading to the separation of MAN‐decorated DGL‐DOX (DD‐M) from the Tf–TfR complex in endo/lysosomes. The detached DD‐M is more prone to escape from endo/lysosomes and can further be exocytosed into brain parenchyma via the mediation of glucose transporter located on the abluminal endothelial membrane. Moreover, the DD‐M in brain parenchyma can target glioma cells. Significantly, the DD‐MCT enters into brain parenchyma in greater amounts, resulting in enhanced accumulation at glioma site and thus improved antiglioma therapeutic outcome. This strategy pioneers a new path for reducing the trapping of nanotherapeutics within BBB endothelium but increasing their transcytosis into brain parenchyma.
Acid‐cleavable transferrin and P‐aminophenyl‐α‐D‐mannopyranoside co‐modified doxorubicin‐tethered dendrigraft poly‐l‐lysine are successfully designed and synthesized. The functional nanoparticles undergo a four‐step journey within endothelial cells for programmed targeting delivery to glioma cells. The nanoparticles exhibit increased transcytosis across blood–brain barriers and precise glioma targeting effect, as well as improved chemotherapeutic outcome.
Nanovaccine‐based immunotherapy (NBI) has received greater attention recently for its potential to prime tumor‐specific immunity and establish a long‐term immune memory that prevents tumor ...recurrence. Despite encouraging results in the recent studies, there are still numerous challenges to be tackled for eliciting potent antitumor immunity using NBI strategies. Based on the principles that govern immune response, here it is proposed that these challenges need to be addressed at the five critical cascading events: Loading tumor‐specific antigens by nanoscale drug delivery systems (L); Draining tumor antigens to lymph nodes (D); Internalization by dendritic cells (DCs) (I); Maturation of DCs by costimulatory signaling (M); and Presenting tumor‐peptide‐major histocompatibility complexes to T cells (P) (LDIMP cascade in short). This review provides a detailed and objective overview of emerging NBI strategies to improve the efficacy of nanovaccines in each step of the LDIMP cascade. It is concluded that the balance between each step must be optimized by delicate designing and modification of nanovaccines and by combining with complementary approaches to provide a synergistic immunity in the fight against cancer.
Nanovaccine‐based immunotherapy has received significant attention recently since it can trigger antigen‐specific immunity and establish long‐term immune memory for preventing tumor recurrence. However, there are still many challenges to be addressed for eliciting potent immunity. This review provides a detailed overview of emerging nanovaccine‐based strategies to improve the efficiency of each process within the challenges in the whole vaccination cascade.
Primary lung cancer is one of the most common malignant tumors in China. Approximately 60% of lung cancer patients have distant metastasis at the initial diagnosis, so it is necessary to find new ...tumor markers for early diagnosis and individualized treatment. Tumor markers contribute to the early diagnosis of lung cancer and play important roles in early detection and treatment, as well as in precision medicine, efficacy monitoring, and prognosis prediction. The pathological diagnosis of lung cancer in small biopsy specimens determines whether there are tumor cells in the biopsy and tumor type. Because biopsy is traumatic and the compliance of patients with multiple biopsies is poor, liquid biopsy has become a hot research direction. Liquid biopsies are advantageous because they are nontraumatic, easy to obtain, reflect the overall state of the tumor, and allow for real-time monitoring. At present, liquid biopsies mainly include circulating tumor cells, circulating tumor DNA, exosomes, microRNA, circulating RNA, tumor platelets, and tumor endothelial cells. This review introduces the research progress and clinical application prospect of liquid biopsy technology for lung cancer.
Adrenomedullin (ADM) exerts anti-oxidant, anti-inflammatory and anti-apoptotic effects in Leydig cells. However, the role and mechanism of ADM in the pyroptosis of Leydig cells are poorly understood. ...This study first showed the protective effects of ADM on the pyroptosis and biological functions of Leydig cells exposed to lipopolysaccharide (LPS) by promoting autophagy. Primary rat Leydig cells were treated with various concentrations of LPS and ADM, together with or without N-acetyl-L-cysteine (NAC) or 3-methyladenine (3-MA). Cell proliferation was detected through CCK-8 and BrdU incorporation assays, and ROS level was measured with the DCFDA assay. Real-time PCR, western blot, immunofluorescence, transmission electron microscopy, TUNEL and flow cytometry were performed to examine ADM's effect on the pyroptosis, autophagy and steroidogenic enzymes of Leydig cells and AMPK/mTOR signalling. Like NAC, ADM dose-dependently reduced LPS-induced cytotoxicity and ROS overproduction. ADM also dose-dependently ameliorated LPS-induced pyroptosis by reversing the increased expression of NLRP3, ASC, caspase-1, IL-1β, IL-18, GSDMD, caspase-3, caspase-7, TUNEL-positive and PI and active caspase-1 double-stained positive rate, DNA fragmentation and LDH concentration, which could be rescued via co-incubation with 3-MA. ADM dose-dependently increased autophagy in LPS-induced Leydig cells, as confirmed by the increased expression of LC3-I/II, Beclin-1 and ATG-5; decreased expression of p62 and autophagosomes formation; and increased LC3-II/LC3-I ratio. However, co-treatment with 3-MA evidently decreased autophagy. Furthermore, ADM dose-dependently rescued the expression of steroidogenic enzymes, including StAR, P450scc, 3β-HSD and CYP17, and testosterone production in LPS-induced Leydig cells. Like rapamycin, ADM dose-dependently enhanced AMPK phosphorylation but reduced mTOR phosphorylation in LPS-induced Leydig cells, which could be rescued via co-incubation with 3-MA. In addition, pyroptosis was further decreased, and autophagy was further promoted in LPS-induced Leydig cells upon co-treatment with ADM and rapamycin. ADM may protect the steroidogenic functions of Leydig cells against pyroptosis by activating autophagy via the ROS-AMPK-mTOR axis.
Summary
Influence lines (ILs) have recently been proposed as an emerging index for structural damage localization. This study investigated a novel damage detection and quantification method that is ...based on deflection ILs (DILs) for beam structures. By reconstructing DIL matrices using a matrix decomposition method, the relationship between structural damage and changes in DILs was revealed. Subsequently, a DIL‐based method was proposed to locate and quantify damage in beam structures. Numerical case studies and laboratory experiments were conducted to investigate the effectiveness of the proposed method in different scenarios, namely, single or multiple damages in a one‐span simply supported beam and a two‐span continuous beam. Satisfactory damage localization and quantification results were achieved even with noise interference in the measurements. The influences of sensor number and locations were also examined in the numerical studies. In general, an increasing number of sensors and short distance from the measurement points to the damage locations benefited the damage detection accuracy. The numerical and experimental results demonstrated that the proposed DIL‐based method will be a promising field detection technique for the localization and quantification of damage in bridges.
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