The aim of the experiment is to study in vitro regeneration efficiency of international potato center (CIP)-bred salt tolerant potato genotypes under salt stress condition and to identify effective ...potato genotype(s) for saline belt areas of Bangladesh. An in vitro shoot bioassay of eight CIP-bred potato genotypes viz. CIP 102, CIP 106, CIP 111, CIP 117, CIP 124, BARI Alu 72 (CIP 139), and BARI Alu 73 (CIP 127) and CIP 136 were used. In this study, single node of these genotypes was cultured in MS media supplemented with 0, 80, 100, 120, 140 and 160 mM NaCl. Among the eight genotypes, BARI Alu 72 (CIP 139) showed the highest tolerance against salinity up to 160 mM NaCl (14.61 dS/m) for all studied parameters (except shoot and root initiation) with the highest plant height (9.67 cm), leaves number (13.60), nodes number (9.50), root length (6.50 cm), roots number (7.80), fresh weight of shoot (536.1 mg) and root (205.60 mg). On the other hand, CIP 106 was found the most susceptible genotype against salinity showing its highest salinity tolerance up to 120 mM NaCl (10.96 dS/m) with maximum plant height (7.17 cm), leaves number (12.50), nodes number (6.50), root length (7.50 cm), roots number (9.7), fresh weight of shoot (572.3 mg) and root (250 mg). The experiment's findings corroborated CIP's findings that they were salt tolerant, as well as recommended for their cultivation suitability in saline-affected area in Bangladesh.
Foot and mouth disease (FMD) is a highly contagious, transboundary, and re-emerging viral disease that mostly affects cloven-hoofed animals specially cattle, goats, swine, sheep, etc. resulting in ...significant economic losses. Despite regular vaccination, outbreaks of the disease have become a yearly occurrence across the country. RT-PCR was used to determine the prevalence and molecular detection of serotype A of FMDV in clinically affected goats in Goat research farm, BLRI, Savar, Dhaka, Bangladesh during August 2018. A total of 9 samples were taken from 1 to 2 years old Black Bengal goats suspected to have FMD. FMDV was detected in 55.56% (5/9) of the suspected samples using RT-PCR. The serotype of positive samples was also determined using gsRT-PCR. However, FMDV serotype A was prevalent in 100% (5) positive samples. Additionally, considering the age, the prevalence of confirmed FMD outbreak was 40% (2), 40% (2), and 20% (1) at the age of 1, 1.5 and 2 years, respectively. It was found that young goats are more susceptible to FMDV than adults. However, it can be concluded that suspected goats were infected with FMDV serotype A and trivalent FMD vaccine is suggested for prevention and control of FMD outbreak.
Asian J. Med. Biol. Res. 2022, 8 (1), 30-36
Cancer is a class of diseases characterized by uncontrolled cell growth. The current treatment options of cancer are radiotherapy, chemotherapy, hormone therapy, and surgery, where all of them have ...unpleasant side effects. Due to their adverse side effects, it is challenging to develop new drug for cancer treatment. Hence, the scientists are trying to seek for noble compounds from natural sources to treat cancer. Therefore, in the present investigation, a widely consumable vegetable Basella alba was subjected to evaluate its antiproliferative effect along with molecular signaling of apoptosis in Ehrlich ascites carcinoma (EAC) cell line. Cell growth inhibition was determined by haemocytometer whereas apoptosis of cancer cells were studied by florescence microscope using Hoechst-33342 stain and result was supported by DNA fragmentation and certain cancer related genes expression through PCR analysis. B. alba leaf and seed extract exhibit a considerable scavenging activity in comparison to a standard antioxidant BHT. Moreover, the leaf and seed extracts were able to agglutinate 2% RBC of goat blood at minimum 12.5μg/ml and 50.0μg/ml concentration, respectively. A significant cytotoxic activity was also found in both leaf and seed extract. In haemocytometic observation, the leaf and seed extracts exhibit about 62.54±2.41% and 53.96±2.34% cell growth inhibition, respectively, whereas standard anticancer drug Bleomycin showed 79.43±1.92% growth inhibition. Morphological alteration under fluorescence microscope showed nuclear condensation and fragmentation which is the sign of apoptosis. Apoptosis induction was also confirmed by DNA laddering in leaf and seed treated EAC cells. Upregulation of the tumor suppressor gene P53 and downregulation of antiapoptotic gene Bcl-2 enumerate apoptosis induction. Therefore, current study manifested that leaf and seed extracts of B. alba have antiproliferative activity against EAC cell line and can be a potent source of anticancer agents to treat cancer.
Microplastic (MP) pollution in aquatic systems poses a great threat, as these tiny particles contaminate water, harm marine life, and may eventually enter the food chain, endangering ecosystems and ...human health. The purpose of this study was to assess the presence of MPs in surface-level water samples taken from Chattogram Coastal Area of the Bay of Bengal in Bangladesh. A total of 20 water samples were collected from four sea beaches, spanning the period from September to December 2023. A total of 452 MPs were enumerated from the collected water samples, with 29% observed in Kattoli Beach, 26% in Patenga Beach, 24% in Anowara Beach, and 21% in Banshkhali Beach. MPs ranging from 1-5mm in size were identified as the most prevalent in the study areas. Anowara Beach exhibited a dominant composition of fibers (53%), Banshkhali Beach was characterized by particles (55%), and Patenga Beach and Kattoli Beach displayed a high abundance of fragments (65% and 56%, respectively). The abundance of MPs at Kattoli Beach was significantly high (p<0.05) compared to other beaches. The pollution load index ranged from 1.25-1.49 with the highest index values at Kattoli Beach. The results from this study could be applied as a guide to efficient environmental management for the long-term health of the beaches by reducing the degree of MP load from the coastal and marine ecosystems of Bangladesh.
This research explores the impact of personal factors on resistance to technology adoption in university libraries in Bangladesh, particularly among LIS (Library and Information Science) ...professionals. The study aims to reveal specific challenges and opportunities influencing attitudes toward technology integration, offering a nuanced understanding of resistance factors in this unique cultural and educational context. Through a qualitative case study with 21 LIS professionals from seven university libraries, conducted via semi-structured interviews guided by a questionnaire, recurring themes such as fear of job displacement, perceived technological self-efficacy, and concerns about disruptions to workflows emerge. Fear of job displacement refers to professionals’ apprehension about potential job loss due to technological advancements, while perceived technological self-efficacy reflects individuals’ confidence in using and adapting to new technologies. Concerns about disruptions to workflows highlight worries regarding the impact of technology implementation on existing work processes. Despite challenges, participants acknowledge potential benefits, emphasizing improved services. The study advocates for crucial strategies like comprehensive training programs and inclusive decision-making processes to alleviate resistance. This research provides a focused exploration, original insights, and practical value for advancing technology adoption in university libraries in Bangladesh, contributing to a deeper comprehension of complexities in technology integration within academic institutions.
The experiment was conducted at the Horticulture Farm of the Department of Horticulture, Bangladesh Agricultural University, Mymensingh to explore the influence of seedling age and mulching materials ...on growth and yield of broccoli. The experiment consisted of three seedling ages viz., 21, 28, 35 days and five mulching materials viz., control (Non-mulch), black polythene, rice straw, water hyacinth and white polythene. The experiment was laid out following randomized complete block design with three replications. Results showed that the highest plant height (34.91 cm), number of leaves (15.81), yield (12.19 t/ha) were recorded in 35-day-old seedling (A3 treatment). Among the mulching treatments, the highest plant height (33.55 cm), number of leaves (16.66), yield (11.11 t/ha) were recorded under black polythene mulch (T2 treatment) while non-mulch control condition (T1 treatment) performed inferior on most of the studied parameters. Regarding the combined effects of seedling age and mulching materials, it was observed that maximum number of leaves (16.80) and highest plant height (36.58 cm). Maximum diameter of primary curd (15.60 cm), minimum days to curd initiation (64.39 days after transplanting), weight of primary curd (410.31 g), fresh weight of leaves (745.33 gm), dry matter of primary curd (19.43%), yield (13.41 t/ha) were obtained from A3T2 treatment (35-day-old seedling with black polythene) and the lowest were recorded from A1T1 (21-day-old seedling with non-mulch control condition) treatment. It was found that 35-day-old seedling with black polythene performed superior on plant growth and yield of broccoli whereas the lowest results obtained from 21-day-old seedling with non-mulch control condition. However, it can be concluded that the highest production of broccoli is obtained from 35-day-old seedling and black polythene mulch. Fundam Appl Agric 2021; 6(2.000): 134-143
The study was conducted to develop a technique for early diagnosis of pregnancy in goats from July 2016 to June 2017. About 100 goats of different age groups were randomly selected for the trial and ...goats were categorized under four groups according to their gestation lengths (0.5 to 1 month, >1 to 1.5 months, >1.5 to 2 months and >2 to 2.5 months). Blood from jugular vein and morning urine samples were collected from four groups and brought to the laboratory, and serum was separated from the clotted blood. Then 1%, 1.5% and 2% barium chloride solution were prepared and test was done by using 1 ml barium chloride of each concentration mixing with 1 ml of urine sample, and allowed for 5 and 10 minutes for interpretation. Presence of precipitation was interpreted as negative for pregnancy and absence of precipitation was interpreted as positive for pregnancy. For each blood sample, 3-5 drops of serum was added in each sample insertion hole of the Bovipreg kit and 5 minutes was allowed to observe the presence of one or two red line in the kit. One red line was interpreted as negative pregnancy and two red lines were interpreted as positive pregnancy. In our study 1% barium chloride, showed 93.5% accuracy, whereas 1.5% barium chloride provided 92% accuracy for the goats having 0.5 to 1 month of gestation. In case of 2% barium chloride, the highest accuracy was 91.5% for the goats having >1 to 1.5 month of gestation. Conversely, the accuracy for progesterone-based early pregnancy diagnostic kit was 96.5%. Therefore, 1% barium chloride can be utilized for the diagnosis of pregnancy in goats.
Asian J. Med. Biol. Res. 2022, 8 (1), 42-46
PDF
