The development of multifunctional particles using polymeric scaffolds is an emerging technology for many nanobiotechnological applications. Here we present a system for the production of ...multifunctional complexes, based on the high affinity non-covalent interaction of cohesin and dockerin modules complementary fused to decameric
Brucella abortus
lumazine synthase (BLS) subunits, and selected target proteins, respectively. The cohesin-BLS scaffold was solubly expressed in high yield in
Escherichia coli
, and revealed a high thermostability. The production of multienzymatic particles using this system was evaluated using the catalytic domain of
Cellulomonas fimi
endoglucanase CenA recombinantly fused to a dockerin module. Coupling of the enzyme to the scaffold was highly efficient and occurred with the expected stoichiometry. The decavalent enzymatic complexes obtained showed higher cellulolytic activity and association to the substrate compared to equivalent amounts of the free enzyme. This phenomenon was dependent on the multiplicity and proximity of the enzymes coupled to the scaffold, and was attributed to an avidity effect in the polyvalent enzyme interaction with the substrate. Our results highlight the usefulness of the scaffold presented in this work for the development of multifunctional particles, and the improvement of lignocellulose degradation among other applications.
Key points
• New system for multifunctional particle production using the BLS scaffold
• Higher cellulolytic activity of polyvalent endoglucanase compared to the free enzyme
• Amount of enzyme associated to cellulose is higher for the polyvalent endoglucanase
Graphical Abstract
Abstract
An incremental version of the fourth catalog of active galactic nuclei (AGNs) detected by the Fermi Large Area Telescope is presented. This version (4LAC-DR3) derives from the third data ...release of the 4FGL catalog based on 12 yr of
E
> 50 MeV gamma-ray data, where the spectral parameters, spectral energy distributions (SEDs), yearly light curves, and associations have been updated for all sources. The new reported AGNs include 587 blazar candidates and four radio galaxies. We describe the properties of the new sample and outline changes affecting the previously published one. We also introduce two new parameters in this release, namely the peak energy of the SED high-energy component and the corresponding flux. These parameters allow an assessment of the Compton dominance, the ratio of the inverse-Compton to the synchrotron-peak luminosities, without relying on X-ray data.
The Fermi Large Area Telescope (LAT) has opened the way for comparative studies of cosmic rays (CRs) and high-energy objects in the Milky Way (MW) and in other, external, star-forming galaxies. Using ...2 yr of observations with the Fermi LAT, Local Group galaxy M31 was detected as a marginally extended gamma-ray source, while only an upper limit has been derived for the other nearby galaxy M33. We revisited the gamma-ray emission in the direction of M31 and M33 using more than 7 yr of LAT Pass 8 data in the energy range 0.1 - 100 GeV , presenting detailed morphological and spectral analyses. M33 remains undetected, and we computed an upper limit of 2.0 × 10 − 12 erg cm − 2 s − 1 on the 0.1 - 100 GeV energy flux (95% confidence level). This revised upper limit remains consistent with the observed correlation between gamma-ray luminosity and star formation rate tracers and implies an average CR density in M33 that is at most half of that of the MW. M31 is detected with a significance of nearly 10 . Its spectrum is consistent with a power law with photon index Γ = 2.4 0.1 stat + syst and a 0.1 - 100 GeV energy flux of ( 5.6 0.6 stat + syst ) × 10 − 12 erg cm − 2 s − 1 . M31 is detected to be extended with a 4 significance. The spatial distribution of the emission is consistent with a uniform-brightness disk with a radius of 0 4 and no offset from the center of the galaxy, but nonuniform intensity distributions cannot be excluded. The flux from M31 appears confined to the inner regions of the galaxy and does not fill the disk of the galaxy or extend far from it. The gamma-ray signal is not correlated with regions rich in gas or star formation activity, which suggests that the emission is not interstellar in origin, unless the energetic particles radiating in gamma rays do not originate in recent star formation. Alternative and nonexclusive interpretations are that the emission results from a population of millisecond pulsars dispersed in the bulge and disk of M31 by disrupted globular clusters or from the decay or annihilation of dark matter particles, similar to what has been proposed to account for the so-called Galactic center excess found in Fermi-LAT observations of the MW.
First Fermi-LAT Solar Flare Catalog Ajello, M.; Baldini, L.; Bastieri, D. ...
The Astrophysical journal. Supplement series,
02/2021, Letnik:
252, Številka:
2
Journal Article
Recenzirano
Odprti dostop
We present the first Fermi-Large Area Telescope (LAT) solar flare catalog covering the 24th solar cycle. This catalog contains 45 Fermi-LAT solar flares (FLSFs) with emission in the γ-ray energy band ...(30 MeV-10 GeV) detected with a significance of ≥5 over the years 2010-2018. A subsample containing 37 of these flares exhibits delayed emission beyond the prompt-impulsive hard X-ray phase, with 21 flares showing delayed emission lasting more than two hours. No prompt-impulsive emission is detected in four of these flares. We also present in this catalog observations of GeV emission from three flares originating from active regions located behind the limb of the visible solar disk. We report the lightcurves, spectra, best proton index, and localization (when possible) for all FLSFs. The γ-ray spectra are consistent with the decay of pions produced by >300 MeV protons. This work contains the largest sample of high-energy γ-ray flares ever reported and provides a unique opportunity to perform population studies on the different phases of the flare and thus allowing a new window in solar physics to be opened.
Gamma Rays from Fast Black-hole Winds Ajello, M.; Baldini, L.; Ballet, J. ...
Astrophysical journal/The Astrophysical journal,
2021, Letnik:
921, Številka:
2
Journal Article
Recenzirano
Odprti dostop
Abstract
Massive black holes at the centers of galaxies can launch powerful wide-angle winds that, if sustained over time, can unbind the gas from the stellar bulges of galaxies. These winds may be ...responsible for the observed scaling relation between the masses of the central black holes and the velocity dispersion of stars in galactic bulges. Propagating through the galaxy, the wind should interact with the interstellar medium creating a strong shock, similar to those observed in supernovae explosions, which is able to accelerate charged particles to high energies. In this work we use data from the Fermi Large Area Telescope to search for the
γ
-ray emission from galaxies with an ultrafast outflow (UFO): a fast (
v
∼ 0.1 c), highly ionized outflow, detected in absorption at hard X-rays in several nearby active galactic nuclei (AGN). Adopting a sensitive stacking analysis we are able to detect the average
γ
-ray emission from these galaxies and exclude that it is due to processes other than UFOs. Moreover, our analysis shows that the
γ
-ray luminosity scales with the AGN bolometric luminosity and that these outflows transfer ∼0.04% of their mechanical power to
γ
-rays. Interpreting the observed
γ
-ray emission as produced by cosmic rays (CRs) accelerated at the shock front, we find that the
γ
-ray emission may attest to the onset of the wind–host interaction and that these outflows can energize charged particles up to the transition region between galactic and extragalactic CRs.
We report on the Fermi-LAT detection of high-energy emission from the behind-the-limb (BTL) solar flares that occurred on 2013 October 11, and 2014 January 6 and September 1. The Fermi-LAT ...observations are associated with flares from active regions originating behind both the eastern and western limbs, as determined by STEREO. All three flares are associated with very fast coronal mass ejections (CMEs) and strong solar energetic particle events. We present updated localizations of the >100 MeV photon emission, hard X-ray (HXR) and EUV images, and broadband spectra from 10 keV to 10 GeV, as well as microwave spectra. We also provide a comparison of the BTL flares detected by Fermi-LAT with three on-disk flares and present a study of some of the significant quantities of these flares as an attempt to better understand the acceleration mechanisms at work during these occulted flares. We interpret the HXR emission to be due to electron bremsstrahlung from a coronal thin-target loop top with the accelerated electron spectra steepening at semirelativistic energies. The >100 MeV gamma-rays are best described by a pion-decay model resulting from the interaction of protons (and other ions) in a thick-target photospheric source. The protons are believed to have been accelerated (to energies >10 GeV) in the CME environment and precipitate down to the photosphere from the downstream side of the CME shock and landed on the front side of the Sun, away from the original flare site and the HXR emission.
RPE65 is essential for the biosynthesis of 11-
cis-retinal, the chromophore of rhodopsin. Here, we show that the membrane-associated form (mRPE65) is triply palmitoylated and is a chaperone for all-
...trans-retinyl esters, allowing their entry into the visual cycle for processing into 11-
cis-retinal. The soluble form of RPE65 (sRPE65) is not palmitoylated and is a chaperone for vitamin A, rather than all-
trans-retinyl esters. Thus, the palmitoylation of RPE65 controls its ligand binding selectivity. The two chaperones are interconverted by lecithin retinol acyl transferase (LRAT) acting as a molecular switch. Here mRPE65 is a palmitoyl donor, revealing a new acyl carrier protein role for palmitoylated proteins. When chromophore synthesis is not required, mRPE65 is converted into sRPE65 by LRAT, and further chromophore synthesis is blocked. The studies reveal new roles for palmitoylated proteins as molecular switches and LRAT as a palmitoyl transferase whose role is to catalyze the mRPE65 to sRPE65 conversion.
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