Učestvovanje u sportskoj rekreaciji postaje sve važnije kako ista ima veliki uticaj na kvalitet ljudskog života; poboljšavajući i opšte zdravlje i fizičko stanje. Ples se ne posmatra samo kao ...profesionalni sport, nego je među ljudima stekao i značajnu popularnost kao vrsta rekreacije. Istraživanje je sprovedeno na 93 kandidata koji pohađaju časove rekreativnog plesa sa ciljem da se otkrije kakva je intenzivnost fizičkog opterećenja i potrošnje energije kod plesača. Podaci prikupljeni uz pomoć Polar Team2 sistema su korišćeni za direknto poređenje razlika u intenzitetu fizičkog opterećenja i potrošnje energije između suprotnih polova. Razlike između intenziteta fizičkog opterećenja i potrošnje energije su određene uz pomoć ANOVA metoda. Ukratko, rezultati su pokazali da su statistički značajne razlike najočiglednije u potrošnji energije, između muških i ženskih plesača. Razlike u intenzitetu fizičkog opterećenja između polova nisu pokazale nikakav statistički značaj.
•OH column removes majority of DNA, protein and media with 100% phage recovery.•PrimaS and H-Bond column substantially reduce endotoxin levels (7 log reduction).•Fast AEX-MALS analytical method can ...be used for bacteriophage quantification.
Bacteriophages represent immense potential as therapeutic agents. Many of the most compelling applications of bacteriophages involve human therapy, some pertinent to gene therapy, others involving antibiotic replacement. Phages themselves are considered safe for humans. However, phage lysates may contain many kinds of harmful by-products, especially endotoxins of gram-negative bacteria and protein toxins produced by many pathogenic bacterial species. In bacteriophage research and therapy, most applications ask for highly purified phage suspensions, as such it is crucial to reduce proteins, endotoxins, DNA and other contaminants.
In this article we present an efficient two-step chromatographic purification method for P. aeruginosa bacteriophage PP-01, using Convective Interaction Media (CIM®) monoliths, that is cGMP compliant and easy to scale-up for most stringent production of the therapeutic phage. First chromatographic step on CIMmultus OH resulted in 100% bacteriophage recovery with a reduction of 98 % protein and more than 99 % DNA content. Polishing was conducted using three different column options, CIMmultus with QA, H-Bond and PrimaS ligands. For PP-01 bacteriophage all three different options worked, but multimodal ligands H-Bond and PrimaS outperformed traditional QA in endotoxin removal (7 log step reduction). Additionally, an HPLC analytical method was developed to estimate phage concentration and impurity profile in different in-process samples. The HPLC method shows good correlation with drop assay titration, provides useful insights and can be run very fast with just 20 min per sample analysis.
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