Perovskite is a promising photosensor material for direct integration on integrated circuit (IC) chips due to the low-temperature growth process. However, the pixeled perovskite integration ...technology for high-resolution imaging faces challenges because of the poor perovskite stability and complex substrates. This work demonstrates a pixeled perovskite integration method on complex substrates with polydopamine (PDA)-assisted hydrophilic-hydrophobic treatment. Compared with conventional hydrophilic-hydrophobic treatment, the rich functional group in PDA can endow patterned wetting-dewetting regions on complex substrates. Besides, the proper band alignment between PDA and perovskite film enables a 6.8-fold suppression of dark current and a detectivity improvement from 1.6 × 1010 to 5.8 × 1010 Jones. We demonstrate the pixeled Cs2AgBiBr6 perovskite photosensor array in SiO2-Au hybrid substrates and confirm the optical imaging function. These results can contribute to the direct integration of photosensors on IC chips in the future.
•A hydrophilic-hydrophobic treatment method is proposed with polydopamine assistance.•Pixeled lead-free perovskite array is fabricated on SiO2-Au complex substrates.•The improved detectivity is obtained in the perovskite-polydopamine photosensor.
We previously associated the cytoskeleton linker protein, Ezrin, with the metastatic phenotype of pediatric sarcomas, including osteosarcoma and rhabdomyosarcoma. These studies have suggested that ...Ezrin contributes to the survival of cancer cells after their arrival at secondary metastatic locations. To better understand this role in metastasis, we undertook two noncandidate analyses of Ezrin function including a microarray subtraction of high-and low-Ezrin-expressing cells and a proteomic approach to identify proteins that bound the N-terminus of Ezrin in tumor lysates. Functional analyses of these data led to a novel and unifying hypothesis that Ezrin contributes to the efficiency of metastasis through regulation of protein translation. In support of this hypothesis, we found Ezrin to be part of the ribonucleoprotein complex to facilitate the expression of complex messenger RNA in cells and to bind with poly A binding protein 1 (PABP1; PABPC1 ). The relevance of these findings was supported by our identification of Ezrin and components of the translational machinery in pseudopodia of highly metastatic cells during the process of cell invasion. Finally, two small molecule inhibitors recently shown to inhibit the Ezrin metastatic phenotype disrupted the Ezrin/PABP1 association. Taken together, these results provide a novel mechanistic basis by which Ezrin may contribute to metastasis.
Curcumin, a yellow pigment derived from Curcuma longa Linn, has been favored by the Eastern as dietary ingredients for centuries. During the past decade, extensive investigations have revealed ...curcumin sensitized various chemotherapeutic agents in human breast, colon, pancreas, gastric, liver, brain and hematological malignant disorders in vivo and in vitro. Several pathways and specific targets including NF-κB, STAT3, COX-2, Akt and multidrug resistant protein have been identified to facilitate curcumin as a chemosensitizer. Recent studies suggest HIF-1α participated in the development of drug resistance in cancer cells and targeting HIF-1α either by RNAi or siRNA successfully overcame chemotherapeutic resistance. To investigate the mechanism basis of curcumin as a chemosensitizer in lung cancer, we examined curcumin's effects on HIF-1α in cis-platin (DDP) sensitive A549 and resistant A549/DDP cell lines by RT-PCR and Western blot. HIF-1α in A549/DDP cells was found to be overexpressed at both mRNA and protein levels together with a poor response to DDP. Results from transient transfection and flow cytometry showed the HIF-1α abnormality contributed to DDP resistance in A549/DDP lung cancer cells. Combined curcumin and DDP treatment markedly inhibited A549/DDP cells proliferation, reversed DDP resistance and triggered apoptotic death by promoting HIF-1α degradation and activating caspase-3, respectively. Expression of HIF-1α-dependent P-gp also seemed to decrease as response to curcumin in a dose-dependent manner. Our findings shed light on drug resistant reversing effect of curcumin in lung cancer cells by inhibiting HIF-1α expression and activating caspase-3.
Implant‐related infection (IRI) is a devastating complication in orthopedic procedures. Traditional materials used in orthopedics are susceptible to bacterial infection. In this study, we developed a ...copper‐bearing 316L stainless steel (316L‐Cu SS) for the prevention of IRI. This 316L‐Cu SS allowed stable and continuous release of copper ions with a rate of 5.079 ng/cm2/day. Compared with 316L stainless steel (316L SS), 316L‐Cu SS exhibited a broad‐spectrum antibacterial effect against Staphylococcus aureus, Escherichia coli, and Staphylococcus epidermidis with the bacterial reduction percentages of 95.2, 94.8, and 94.1%, respectively. The antibiofilm activity was confirmed by crystal violet assay, scanning electron microscopy, and confocal laser scanning microscopy. The in vivo antibacterial performance was tested on a rat model. When nails were treated with a low concentration of bacteria, 316L SS group exhibit a bone infection with a radiographic score of 8.9 ± 1.1 and a histological score of 10.4 ± 1.0, which were higher than 316L‐Cu SS group (1.2 ± 0.2 and 0.9 ± 0.2), indicating IRI was reduced by 316L‐Cu SS. When nails were treated with a high concentration of bacteria, IRI was also alleviated by 316L‐Cu SS. Together, these results demonstrated that 316L‐Cu SS is a promising material for preventing IRI.
A new series of diaryl urea derivatives bearing N-acylhydrazone moiety were designed and synthesized. All the target compounds were evaluated for their antiproliferative activities against human ...leukemia cell line （HL-60）, human lung adenocarcinoma epithelial cell line （A549） and human breast cancer cell line （MDA-MB-231） in vitro by standard MTT assay. The pharmacological results indicated that some compounds exhibited promising antitumor activities. Compound 1j showed the most potent antiproliferative activity against the tested three cell lines with IC50 values of 0.13 μmol/L, 0.7 μmol/L and 0.5μmol/L, respectively.
In order to construct the multimedia remote interactive operations for the locked-in patients, this paper proposed a novel BCI system that constructed by the EEG signals with convolutional neural ...network. To constructing the remote interactive operations, the temporal and spatial features of electroencephalogram (EEG) signals were extracted by longitudinal convolution kernel and transverse convolution kernel respectively. The classification of motion imagination was completed by using two fully-connected layers. Finally, the classification results will be transferred as the multimedia remote interactive results in response to the multimedia remote interactive operations. The experimental results showed that the proposed EEG-BCI system had a good performance and efficiency for the multimedia remote interactive operations, which will facilitate the fields of multimedia remote interactive operations.
•HKUST-1 coated capillary were fabricated by in situ layer-by-layer self-assembly method.•The multilayer coated capillary columns were characterized by SEM, XRD and ICP-AES.•Neutral analytes were ...baseline separated on 10, 15, 20layers coated capillary columns.•Phenolic analytes were baseline separated on 10, 15 layers coating capillary columns.•10, 15, 20 layers coating capillary columns were used for more than 120 runs.
The popularity of novel nanoparticles coated capillary column has aroused widespread attention of researchers. Metal organic frameworks (MOFs) with special structure and chemical properties have received great interest in separation sciences. This work presents the investigation of HKUST-1 (Hong Kong University of Science and Technology-1, called Cu3(BTC)2 or MOF-199) nanoparticles as a new type of coating material for capillary electrochromatography. For the first time, three layers coating (3-LC), five layers coating (5-LC), ten layers coating (10-LC), fifteen layers coating (15-LC), twenty layers coating(20-LC) and twenty-five layers coating (25-LC) capillary columns coated with HKUST-1 nanoparticles were synthesized by covalent bond with in situ, layer-by-layer self-assembly approach. The results of scanning electron microscopy (SEM), X-ray diffraction (XRD) and plasma atomic emission spectrometry (ICP-AES) indicated that HKUST-1 was successfully grafted on the inner wall of the capillary. The separating performances of 3-LC, 5-LC, 10-LC, 15-LC, 20-LC and 25-LC open tubular (OT) capillary columns were studied with some neutral small organic molecules. The results indicated that the neutral small organic molecules were separated successfully with 10-LC, 15-LC and 20-LC OT capillary columns because of the size selectivity of lattice aperture and hydrophobicity of organic ligands. In addition, 10-LC and 15-LC OT capillary columns showed better performance for the separation of certain phenolic compounds. Furthermore, 10-LC, 15-LC and 20-LC OT capillary columns exhibited good intra-day repeatability with the relative standard deviations (RSDs; %) of migration time and peak areas lying in the range of 0.3–1.2% and 0.5–4.2%, respectively. For inter-day reproducibility, the RSDs of the three OT capillary columns were found to be lying in the range of 0.3–5.5% and 0.3–4.5% for migration time and peak area, respectively. The RSDs of retention times for column-to-column for three batches of 10-LC, 15-LC and 20-LC OT capillary columns were in the range from 2.3% to 7.2%. Moreover, the fabricated 10-LC, 15-LC and 20-LC OT capillary columns exhibited good repeatability and stability for separation, which could be used successively for more than 120 runs with no observable changes on the separation efficiency.
A rapid and sensitive competitive fluorescence-linked immunosorbent assay (cFLISA) based on quantum dot−streptavidin conjugate (QDs−SA) was developed for the detection of chlorpyrifos in drinking ...water. The QDs−SA conjugate, which consists of 3-mercaptopropyl acid-stabilized CdTe nanoparticle QDs and streptavidin (SA) made through the active ester method, was employed to improve the sensitivity of QDs−SA-cFLISA. The 50% inhibition concentration (IC50) and the limit of detection (LOD) were 28.5 and 3.8 ng mL−1, respectively. QDs−SA-cFLISA increased sensitivity 5.5-fold and reduced detection time by 1 h compared with conventional enzyme-linked immunosorbent assay (ELISA). With chlorpyrifos concentrations of 100, 50, and 20 ng mL−1, recoveries ranged from 85.9% to 105.3% with coefficients of variation ranging from 6.3% to 13.5%. This study demonstrated that QDs−SA-cFLISA was more rapid and sensitive than conventional ELISA. Therefore, it can be used as a novel screening method for the detection of pesticide residues.
This article is devoted to estimating simultaneously both the states and the inputs of linear time-invariant fractional-order systems (LTI-FOSs) with the order
0
<
α
<
2
. Firstly, a necessary and ...sufficient stability criterion for LTI-FOSs with the order
0
<
α
<
1
is derived by the linear matrix inequality technique. Secondly, a novel fractional-order observer combined with state vectors and ancillary vectors is given, which can generalize several forms of existing observers. Moreover, the parameter matrices of the desired observer for both the order
0
<
α
<
1
and
1
<
α
<
2
are solved on the basis of the stability theorem and the solution to the generalized inverse matrix. Finally, the fractional-order observer design algorithm is proposed and then applied to an illustrated example, in which the simulation results are reported to verify the effectiveness of the proposed approach.
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