Hotel Room-inventory Management Toh, Rex S.; Dekay, Frederick
Cornell Hotel and Restaurant Administration Quarterly,
08/2002, Letnik:
43, Številka:
4
Journal Article
Category:
Basic Sciences/Biologics
Introduction/Purpose:
New approaches to improve tendon repair after injury are an active area of research. Critical properties of tendons are governed by the ...production and assembly of fibrillar collagens. Cross-linking of fibrillar collagen is a primary factor in determining the function and mechanical properties of the collagen fibers comprising Enzymatic cross-linking by lysyl oxidase in the telopeptide domain of collagen I and III is one determinant of collagen fibril assembly and is the best characterized biochemical cross-link. Transglutaminase catalyzes the modification of lysine residues that in turn form an n-e-glutamyl lysine bond between proteins in the extracellular space. We hypothesize that transglutaminase-dependent modification of collagen in tendons is also a principal determinant of tendon strength and function and is dependent upon tension.
Methods:
3-D collagen gels were generated from acid solubilized type I collagen with telopeptides (Advanced BioMatrix). Collagen gels were plated and loaded into a MechanoCulture FX apparatus (CellScale). Gels were subjected to a 10% stretch for 24 hrs at 37°C at 2hz (dynamic) or no stretch, static controls. Gels exposed to enzymatic cross-linking were incubated with either 2.4 ng of recombinant Transglutaminase 2 (Axxora) in a 10 mM Ca2+ solution. Inhibition and labeling of transglutaminase substrates was performed by incubation of collagen gels with 0.2 mM aminopentyl biotinamide in DMSO. Soluble collagen was separated from insoluble collagen by centrifugation at 10,000G. Insoluble fractions were boiled in SDS-Laemmli buffer prior to separation by SDS-PAGE. Collagen in soluble and insoluble fractions was evaluated by Coomassie stain whereas transglutaminase modification was detected via western blot using streptavidin conjugated horse radish peroxidase to detect biotinylated proteins.
Results:
Evaluation of collagen gels subjected to dynamic versus static stretch revealed minor differences in insoluble collagen incorporation in the two conditions. Notably, higher molecular weight cross-linked forms of collagen appeared to be higher in dynamic versus static gels. In the presence of transglutaminase, differences in higher molecular weight cross-linked forms of collagen, beta-bands, were also detected. Finally, incorporation of biotinylated transglutaminase substrate into collagen alpha bands was enriched in dynamic versus static cultures. Hence, preliminary results support a differential role for transglutaminase modification in collagen under cyclic tension versus static conditions.
Conclusion:
A better understanding of the role of dynamic stretching and differential tension in the regulation of collagen cross- link formation is predicted to contribute to improved strategies to treat injured tendons.
We report our preliminary experience with the use of preformed “peel‐away” guiding sheaths and “side‐wire” pacing leads for permanent biventricular pacemaker insertion in 13 patients with heart ...failure. Three of these patients were undergoing an upgrade of a preexistent VVIR pacing system after prior His ablation for medically refractory atrial fibrillation. Six of the patients had undergone attempted biventricular pacemaker insertion, but required left ventricular lead repositioning after total implantation failure or late displacement of the lead. The remaining patients were undergoing new system implantation. Target vessel cannulation was achieved in all patients. However, in one patient, diaphragmatic pacing throughout the target vessel length prevented successful implantation. All other implants were ultimately successful (92% success rate). We conclude that device implantation using a preformed sheath and side‐wire pacing lead is feasible and may offer significant benefits over implantation with currently available technology.
Despite his importance, no substantial study has been devoted to the career of Abū 'l-'Abbās Ahmad b. Idrīs al-Hasanī al-'Arā'ishī al-Fāsī (d. 1837); most accounts of him appear by way of a preface ...to studies of his pupils. And yet through his teachings, pupils, and family, he was undoubtedly one of the key religious figures of the early 19th century Arab Muslim world. Indeed, his influence, direct and indirect, appears to have stretched from North Africa to Indonesia. Three of his pupils from his immediate circle established major brotherhoods, the Sanūsiyya, Khatmiyya, and Rāshidiyya, from which stemmed several other orders.
Asian Contagion: impact on Singapore tourism Khan, Habibullah; Toh, Rex S; Fathima, Khurrath
Annals of tourism research,
2001, 2001-1-00, Letnik:
28, Številka:
1
Journal Article
In this contribution nuclear structure studies with post-accelerated radioactive ion beams from the REX-ISOLDE facility at CERN are presented. The method employed is y-ray spectroscopy with the ...MINIBALL array following "safe" Coulomb excitation. Recent results concerning the investigation of nuclear shapes are presented and discussed.
Managers of long-term care organizations lack usable information about patterns of stay, because the time spent by residents typically exceeds the length of reporting and reimbursement periods. ...Formulas for estimating lengths of stay often yield unstable estimates of long-term stays. Consequently, little insight is gained into changes that may be occurring in stay patterns as the organization adapts its service processes to shifts in markets. An alternative length of stay statistic is proposed from which patterns of stay can be more clearly discerned. Examples utilizing both simulated and field data are presented to highlight the advantages associated with the new statistic.
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