ABSTRACT
In this paper, we present CALSAGOS: Clustering ALgorithmS Applied to Galaxies in Overdense Systems which is a PYTHON package developed to select cluster members and to search, find, and ...identify substructures. CALSAGOS is based on clustering algorithms, and was developed to be used in spectroscopic and photometric samples. To test the performance of CALSAGOS, we use the S-PLUS’s mock catalogues, and we found an error of 1–6 per cent on member selection depending on the function that is used. Besides, CALSAGOS has a F1-score of 0.8, a precision of 85 per cent and a completeness of 100 per cent in the identification of substructures in the outer regions of galaxy clusters (r > r200). The F1-score, precision, and completeness of CALSAGOS fall to 0.5, 75, and 40 per cent when we consider all substructure identifications (inner and outer) due to the function that searches, finds, and identifies the substructures works in 2D, and cannot resolve the substructures projected over others.
The control of apoptosis in mammals has been historically associated with the activity of the BCL-2 family of proteins at the mitochondria. In the past years, a novel group of cell death regulators ...have emerged, known as the Transmembrane BAX Inhibitor-1 Motif-containing (TMBIM) protein family. This group of proteins is composed of at least six highly conserved members expressed in mammals, with homologs in insects, fish, plants, viruses and yeast. Different studies indicate that all TMBIM family members have inhibitory activities in different setting of apoptosis. Here, we overview and integrate possible mechanisms underlying the impact of the TMBIM protein family in the regulation of cell death, which include activities at diverse subcellular compartments, including death receptor regulation, modulation of endoplasmic reticulum (ER) calcium homeostasis, ER stress signaling, autophagy, reactive oxygen species production, among other effects. The possible intersection between the BCL-2 and TMBIM family in the control of cell death is also discussed, in addition to their implication in the progression of cancer.
Les hommes ayant des relations sexuelles avec des hommes (HSH) constituent une population clé du virus de l’immunodéficience humaine (VIH) en Haïti. Cependant, peu de données existent sur cette ...population et les facteurs associés à cette infection. Notre étude s’intéresse aux facteurs liés à un test rapide positif au VIH chez les HSH dans le cadre d’un dispositif de dépistage communautaire en Haïti entre 2015 et 2018. Parmi les 1 416 HSH dépistés, un tiers déclaraient leur premier test VIH et 7,0 % avaient un résultat positif. Avec un âge médian de 25 ans 21–29, plus de la moitié d’entre eux vivaient en milieu urbain (60,7 %) et étaient en situation de précarité financière (68,6 %). Une analyse multivariée a montré que deux facteurs étaient significativement associés à un résultat positif au VIH : avoir eu une infection sexuellement transmissible dans les 12 derniers mois, facteur accentué lorsqu’il est combiné à une consommation de produits psychoactifs ; la pratique du sexe transactionnel dans les 12 derniers mois, facteur accentué par l’appartenance à la classe d’âge des 18–20 ans. Ces résultats doivent se traduire dans la mise en place de futurs programmes et services de prévention du VIH vers les jeunes HSH en Haïti.
A novel Janus micromotor-based strategy for the direct determination of diphenyl phthalate (DPP) in food and biological samples is presented. Mg/Au Janus micromotors are employed as novel analytical ...platforms for the degradation of the non-electroactive DPP into phenol, which is directly measured by difference pulse voltammetry on disposable screen-printed electrodes. The self-movement of the micromotors along the samples result in the generation of hydrogen microbubbles and hydroxyl ions for DPP degradation. The increased fluid transport improves dramatically the analytical signal, increasing the sensitivity while lowering the detection potential. The method has been successfully applied to the direct analysis of DPP in selected food and biological samples, without any sample treatment and avoiding any potential contamination from laboratory equipment. The developed approach is fast (∼5 min) and accurate with recoveries of ∼100%. In addition, efficient propulsion of multiple Mg/Au micromotors in complex samples has also been demonstrated. The advantages of the micromotors-assisted technology, i.e., disposability, portability, and the possibility to carry out multiple analysis simultaneously, hold considerable promise for its application in food and biological control in analytical applications with high significance.
Jets and Mirror Mode Waves in Earth's Magnetosheath Blanco‐Cano, X.; Rojas‐Castillo, D.; Kajdič, P. ...
Journal of geophysical research. Space physics,
July 2023, 2023-Jul, 2023-07-00, 20230701, Letnik:
128, Številka:
7
Journal Article
Recenzirano
Odprti dostop
Magnetosheath jets are localized plasma structures with high dynamic pressure which are frequently observed downstream of the Earth's bow shock. In this work we analyze Magnetospheric MultiScale ...magnetic field and plasma data and show that jets can be found in the quasi‐perpendicular magnetosheath in regions permeated by Mirror mode waves (MMWs). We show that structures identified as jets by their enhanced dynamic pressure can have very different internal structure, with variable signatures in magnetic field magnitude and components, velocity, and density and can be associated to ion distribution functions of various types. This suggests that jets observed in the quasi‐perpendicular magnetosheath are generated by different mechanisms. We find that jets can be related to traveling foreshocks, flux transfer events, and some have MMWs inside them. Our results suggest that some jets have a local source and their formation does not depend on upstream structures. We find that different types of ion distributions can exist inside the jets, while in some cases anisotropic distributions are present, in others counterstreaming distributions exist. We also show that for jets with MMWs inside them, ion distributions can be modulated. This highlights the importance of using ion distributions to identify and classify different types of jets.
Key Points
We study high speed magnetosheath jets coexisting with mirror mode waves
We use Magnetospheric MultiScale data to show that high speed magnetosheath jets have a much more complex structure than previously thought. Velocity distribution functions can be a tool to describe the micro‐physics inside jets
High speed jets in the quasi‐perpendicular magnetosheath could have different generation mechanisms that those in the quasi‐parallel sheath
Abstract
The type I interferon (IFN) signaling pathway has important functions in resistance to viral infection, with the downstream induction of interferon stimulated genes (ISG) protecting the host ...from virus entry, replication and spread.
Listeria monocytogenes
(
Lm
), a facultative intracellular foodborne pathogen, can exploit the type I IFN response as part of their pathogenic strategy, but the molecular mechanisms involved remain unclear. Here we show that type I IFN suppresses the antibacterial activity of phagocytes to promote systemic
Lm
infection. Mechanistically, type I IFN suppresses phagosome maturation and proteolysis of
Lm
virulence factors ActA and LLO, thereby promoting phagosome escape and cell-to-cell spread; the antiviral protein, IFN-induced transmembrane protein 3 (IFITM3), is required for this type I IFN-mediated alteration.
Ifitm3
−/−
mice are resistant to systemic infection by
Lm
, displaying decreased bacterial spread in tissues, and increased immune cell recruitment and pro-inflammatory cytokine signaling. Together, our findings show how an antiviral mechanism in phagocytes can be exploited by bacterial pathogens, and implicate IFITM3 as a potential antimicrobial therapeutic target.
Attention-deficit/hyperactivity disorder (ADHD) diagnosis is based on reported symptoms, which carries the potential risk of over- or under-diagnosis. A biological marker that helps to objectively ...define the disorder, providing information about its pathophysiology, is needed. A promising marker of cognitive states in humans is pupil size, which reflects the activity of an 'arousal' network, related to the norepinephrine system. We monitored pupil size from ADHD and control subjects, during a visuo-spatial working memory task. A sub group of ADHD children performed the task twice, with and without methylphenidate, a norepinephrine-dopamine reuptake inhibitor. Off-medication patients showed a decreased pupil diameter during the task. This difference was no longer present when patients were on-medication. Pupil size correlated with the subjects' performance and reaction time variability, two vastly studied indicators of attention. Furthermore, this effect was modulated by medication. Through pupil size, we provide evidence of an involvement of the noradrenergic system during an attentional task. Our results suggest that pupil size could serve as a biomarker in ADHD.
In a central force system the angle between two successive passages of a body through pericenters is called the apsidal angle. In this paper we prove that for central forces of the form
f
(
r
)
∼
λ
r
...-
(
α
+
1
)
with
α
<
2
the apsidal angle is a monotonous function of the energy, or equivalently of the orbital eccentricity.
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•- Analysis of plastic additives in mussels from two different harvesting areas.•- Plastic additives contamination in raw, steamed and canned mussels were compared.•- MSPD ...methodologies were validated for steamed and canned mussel samples.•- Processed have a positive impact in terms of dietary exposure to plastic additives.•- Food safety implications of processing food commodities contaminated with microplastics.
Microplastics are a complex mix of chemicals containing polymers and certain plastic additives such as bisphenols and phthalates. These particles are porous materials that can also sorb contaminants from their surroundings, and leach chemicals from the particle under certain circumstances. Aquatic animals can ingest microplastic particles, which mostly bioaccumulate in the gastrointestinal tract of animals. In terms of dietary exposure, small animals consumed whole such as mussels, contribute more to the dietary intake of microplastic particles. Plastic additives and contaminants are not chemically bound to the polymers, and certain processing methods or cooking processes result in the release of these chemicals that leach from the plastic particles, leaving them more available for absorption when ingested. Analytical methods are crucial for a better understanding of the occurrence of plastic additives and contaminants in aquatic products, and to know certain circumstances and treatments that influence human exposure. This study uses an MSPD-HPLC methodology for the simultaneous determination of 9 analytes (BPA, BPF, BPS, DEP, DBP, DEHP, DDD, DDT, and DDE) analyzing, for the first time, the occurrence of these chemicals in raw, steamed and canned mussels of two different harvesting areas (Atlantic and the Mediterranean), becoming one of the most efficient methodologies for determining the presence of these analytes in very complex food matrices, able to define the changes in cooking and processing activities. The results showed that the heat and pressure treatment could influence the migration of plastic additives from microplastic particles present in mussels to the cooking liquids.
A multicountry monkeypox disease (MPX) outbreak began in May 2022 in Europe, leading to the assessment as a potential Public Health Emergency of International Concern (PHEIC) on June 23, 2022. Some ...observational studies have partially characterised clinical features, hospitalisations, and deaths. However, no systematic reviews of this MPX outbreak have been published.
We performed a systematic review with meta-analysis, using five databases to assess clinical features, hospitalisations, complications and deaths of MPX confirmed or probable cases. Observational studies, case reports and case series, were included. We performed a random-effects model meta-analysis to calculate the pooled prevalence and 95% confidence interval (95% CI). In addition, we carried out a subgroup analysis according to the continents and a sensitivity analysis excluding studies classified as having a high risk of bias.
A total of 19 articles were included, using only 12 articles in the quantitative synthesis (meta-analysis). For 1958 patients, rash (93%, 95% CI 80-100%), fever (72%, 95% CI 30-99%), pruritus (65%, 95% CI 47-81%), and lymphadenopathy (62%, 47-76%), were the most prevalent manifestations. Among the patients, 35% (95% CI 14-59%) were hospitalised. Some 4% (95% CI 1-9%) of hospitalised patients had fatal outcomes (case fatality rate, CFR).
MPX is spreading rapidly, with a third of hospitalised patients, but less than 5% with fatal outcomes. As this zoonotic virus spreads globally, countries must urgently prepare human resources, infrastructure and facilities to treat patients according to the emerging guidelines and the most reliable clinical information.
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