In this paper, we report on the original global fit procedure of synchrotron small-angle X-ray scattering (SAXS) data applied to a model protein, met-myoglobin, in dilute solution during temperature- ...and pressure-induced denaturation processes at pH 4.5. Starting from the thermodynamic description of the protein unfolding pathway developed by Hawley (Hawley, S. A. Biochemistry 1971, 10, 2436), we have developed a new method for analyzing the set of SAXS curves using a global fitting procedure, which allows us to derive the form factor of all the met-myoglobin species present in the solution, their aggregation state, and the set of thermodynamic parameters, with their p and T dependence. This method also overcomes a reasonably poor quality of the experimental data, and it is found to be very powerful in analyzing SAXS data. SAXS experiments were performed at four different temperatures from hydrostatic pressures up to about 2000 bar. As a result, the presence of an intermediate, partially unfolded, dimeric state of met-myoglobin that forms during denaturation has been evidenced. The obtained parameters were then used to derive the met-myoglobin p, T phase diagram that fully agrees with the corresponding phase diagram obtained by spectroscopic measurements.
Context: Insulin resistance (IR) and obesity, especially abdominal obesity, are regarded as central pathophysiological features of a cluster of cardiovascular risk factors (CVRFs), but their relative ...roles remain undefined. Moreover, the differential impact of IR viz. insulin response has not been evaluated.
Objective: The objective of this study was to dissect out the impact of obesity, abdominal obesity, and IR/insulin response on CVRF.
Design: This was a cross-sectional study.
Setting: The study was conducted at 21 research centers in Europe.
Subjects: The study included a cohort of 1308 nondiabetic subjects 718 women and 590 men, age 30–60 yr, body mass index (BMI) 17–44 kg·m−2.
Main Outcome Measures: We measured IR (by a standardized euglycemic insulin clamp), waist girth, insulin response to an oral glucose tolerance test, and major CVRF, and analyzed their associations by multivariate models and factor analysis.
Results: BMI was positively related to all CVRFs. Waist circumference was related to higher blood pressure and serum triglycerides and lower high-density lipoprotein-cholesterol, IR to reduced glucose tolerance, higher free fatty acids, triglyceride and low-density lipoprotein-cholesterol, and lower high-density lipoprotein-cholesterol, and insulin response to higher heart rate, blood pressure and fasting glucose, and the same dyslipidemic profile as IR (P ≤ 0.05 for all). By factor analysis, three main factors (related to IR, age, and fatness, respectively) appeared to underlie this pattern of associations. Each of BMI, waist girth, IR, and insulin response was independently associated with total CVRF load (all P < 0.001).
Conclusions: When IR, fat mass and distribution, and insulin response are measured simultaneously in a large cohort, no one factor stands out as the sole driving force of the CVRF cluster, each being associated with one or more physiological pathways according to known cause-effect relationships.
Quasi-stellar objects (QSOs) whose spectral energy distributions (SEDs) are reddened by dust either in their host galaxies or in intervening absorber galaxies are to a large degree missed by optical ...color selection criteria like the ones used by the Sloan Digital Sky Survey (SDSS). To overcome this bias against red QSOs, we employ a combined optical and near-infrared (near-IR) color selection. In this paper, we present a spectroscopic follow-up campaign of a sample of red candidate QSOs which were selected from the SDSS and the UKIRT Infrared Deep Sky Survey (UKIDSS). The spectroscopic data and SDSS/UKIDSS photometry are supplemented by mid-infrared photometry from the Wide-field Infrared Survey Explorer. In our sample of 159 candidates, 154 (97%) are confirmed to be QSOs. We use a statistical algorithm to identify sightlines with plausible intervening absorption systems and identify nine such cases assuming dust in the absorber similar to Large Magellanic Cloud sightlines. We find absorption systems toward 30 QSOs, 2 of which are consistent with the best-fit absorber redshift from the statistical modeling. Furthermore, we observe a broad range in SED properties of the QSOs as probed by the rest-frame 2 μm flux. We find QSOs with a strong excess as well as QSOs with a large deficit at rest-frame 2 μm relative to a QSO template. Potential solutions to these discrepancies are discussed. Overall, our study demonstrates the high efficiency of the optical/near-IR selection of red QSOs.
Quasi-stellar objects (QSOs) whose spectral energy distributions (SEDs) are reddened by dust either in their host galaxies or in intervening absorber galaxies are to a large degree missed by optical ...color selection criteria like the ones used by the Sloan Digital Sky Survey (SDSS). To overcome this bias against red QSOs, we employ a combined optical and near-infrared (near-IR) color selection. In this paper, we present a spectroscopic follow-up campaign of a sample of red candidate QSOs which were selected from the SDSS and the UKIRT Infrared Deep Sky Survey (UKIDSS). The spectroscopic data and SDSS/UKIDSS photometry are supplemented by mid-infrared photometry from the Wide-field Infrared Survey Explorer. In our sample of 159 candidates, 154 (97%) are confirmed to be QSOs. We use a statistical algorithm to identify sightlines with plausible intervening absorption systems and identify nine such cases assuming dust in the absorber similar to Large Magellanic Cloud sightlines. We find absorption systems toward 30 QSOs, 2 of which are consistent with the best-fit absorber redshift from the statistical modeling. Furthermore, we observe a broad range in SED properties of the QSOs as probed by the rest-frame 2 mu m flux. We find QSOs with a strong excess as well as QSOs with a large deficit at rest-frame 2 mu m relative to a QSO template. Potential solutions to these discrepancies are discussed. Overall, our study demonstrates the high efficiency of the optical/near-IR selection of red QSOs.
Atrial fibrillation (AF) can be treated with percutaneous catheter ablation procedures into the left atrium. Pulmonary veins stenosis (PV) stenosis is a severe complication of this procedure.
we ...report a case of late hemoptysis secondary to severe PV stenosis in a man who underwent AF ablation 9 months before onset of symptoms. He presented four episodes of bleeding and developed an acute respiratory failure (ARF). Parameters of respiratory mechanics and medical investigation did not show any abnormalities. Only computed tomography (CT) angiography showed stenosis of 3 out of 4 native PVs. PV balloon dilatation in all affected PVs and a stent was implanted in 1 of the 3 PVs with full restoration of respiratory function during 1 year follow-up.
PV stenosis may be the underlying cause of recurrent haemoptysis after AF ablation in the presence of normal respiratory parameters. This diagnosis can be confirmed by means of CT angiography and magnetic resonance imaging can provide accurate localization of stenosis.
Dark matter (DM) is one of the major components in the Universe. However, at present its existence is still only inferred through indirect astronomical observations. DM particles can annihilate or ...decay, producing final-state Standard Model pairs that subsequently annihilate into high-energy \(\gamma\)-rays. The dwarf spheroidal galaxies (dSphs) in the Milky Way DM halo have long been considered optimal targets to search for annihilating DM signatures in GeV-to-TeV \(\gamma\)-ray spectra due to their high DM densities (hence high astrophysical factors), as well as the expected absence of intrinsic \(\gamma\)-ray emission of astrophysical origin. For such targets, it is important to compute the amount of DM in their halos in a consistent way to optimize the \(\gamma\)-ray data analysis. Such estimates directly affect the observability of DM signals in dSphs, as well as the DM constraints that can be derived in case of null detection. In this contribution, we present the results on the sensitivity of the Cherenkov Telescope Array (CTA) for DM annihilation and decay searches using planned observations of the Milky Way dSphs. We select the most promising targets among all presently known dwarf satellites, providing new determinations of their expected DM signal. This study shows an improvement of approximately an order of magnitude in sensitivity compared to current searches in similar targets. We also discuss the results in terms of cuspy and cored DM models, and investigate the sensitivity obtained by the combination of observations from different dSphs. Finally, we explore the optimal strategies for CTA observations of dSphs.
The enhanced activity typical of the core of Seyfert galaxies can drive powerful winds where high-energy phenomena can occur. In spite of their high power content, the number of such non-jetted ...active galactic nuclei (AGN) detected in gamma rays is very limited. In this Letter, we report the identification and measurement of the gamma-ray flux from NGC 4151, a Seyfert galaxy located at about 15.8 Mpc. The source is known for hosting ultra-fast outflows (UFOs) in its innermost core through X-ray spectroscopic observations, thereby becoming the first UFO host ever recognized in gamma rays. UFOs are mildly relativistic, wide opening angle winds detected in the innermost parsecs of active galaxies where strong shocks can develop. We interpret the gamma-ray flux as a result of diffusive shock acceleration at the wind termination shock of the UFO and inelastic hadronic collisions in its environment. Interestingly, NGC 4151 is also spatially coincident with a weak excess of neutrino events identified by the IceCube neutrino observatory. We compute the contribution of the UFO to such a neutrino excess and we discuss other possible emission regions such as the AGN corona.
The search for periodicity in the multi-wavelength high variable emission of blazars is a key feature to understand dynamical processes at work in this class of active galactic nuclei. The blazar PG ...1553+113 is an attractive target due to the evidence of periodic oscillations observed at different wavelengths, with a solid proof of a 2.2-year modulation detected in the \(\gamma\)-ray, UV and optical bands. We aim at investigating the variability pattern of the PG 1553+113 X-ray emission using a more than 10-years long light curve, in order to robustly assess the presence or lack of a periodic behavior whose evidence is only marginal so far. We conducted detailed statistical analyses, studying in particular the variability properties of the X-ray emission of PG 1553+113 by computing the Lomb-Scargle periodograms, which are suited for the analyses of unevenly sampled time series, and adopting epoch folding techniques. We find out a modulation pattern in the X-ray light curve of PG 1553+113 with a period of \(\sim\)1.4 years, about 35% shorter than the one observed in the \(\gamma\)-ray domain. Our finding is in agreement with the recent spectro-polarimetric analyses and supports the presence of more dynamical phenomena simultaneously at work in the central engine of this quasar.
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