Point-scanning imaging systems are among the most widely used tools for high-resolution cellular and tissue imaging, benefiting from arbitrarily defined pixel sizes. The resolution, speed, sample ...preservation and signal-to-noise ratio (SNR) of point-scanning systems are difficult to optimize simultaneously. We show these limitations can be mitigated via the use of deep learning-based supersampling of undersampled images acquired on a point-scanning system, which we term point-scanning super-resolution (PSSR) imaging. We designed a 'crappifier' that computationally degrades high SNR, high-pixel resolution ground truth images to simulate low SNR, low-resolution counterparts for training PSSR models that can restore real-world undersampled images. For high spatiotemporal resolution fluorescence time-lapse data, we developed a 'multi-frame' PSSR approach that uses information in adjacent frames to improve model predictions. PSSR facilitates point-scanning image acquisition with otherwise unattainable resolution, speed and sensitivity. All the training data, models and code for PSSR are publicly available at 3DEM.org.
Libraries of stellar spectra are fundamental tools for the study of stellar populations, and both empirical and synthetic libraries have been used for this purpose. In this paper, a new library of ...high resolution synthetic spectra is presented, ranging from the near-ultraviolet (300 nm) to the near-infrared (1.8 mu m). The library spans all the stellar types that are relevant to the integrated light of old and intermediate-age stellar populations in the involved spectral region (spectral types F through M and all luminosity classes). The grid was computed for metallicities ranging from Fe/H = -2.5 to +0.5, including both solar and alpha -enhanced ( alpha /Fe = 0.4) chemical compositions. The synthetic spectra are a good match to observations of stars throughout the stellar parameter space encompassed by the library and over the whole spectral region covered by the computations.
We present a new grid of model photospheres for the SDSS-III/APOGEE survey of stellar populations of the Galaxy, calculated using the ATLAS9 and MARCS codes. New opacity distribution functions were ...generated to calculate ATLAS9 model photospheres. MARCS models were calculated based on opacity sampling techniques. The metallicity (M/H) spans from -5 to 1.5 for ATLAS and -2.5 to 0.5 for MARCS models. There are three main differences with respect to previous ATLAS9 model grids: a new corrected H2O line list, a wide range of carbon (C/M) and alpha element alpha /M variations, and solar reference abundances from Asplund et al. The added range of varying carbon and alpha -element abundances also extends the previously calculated MARCS model grids. Altogether, 1980 chemical compositions were used for the ATLAS9 grid and 175 for the MARCS grid. Over 808,000 ATLAS9 models were computed spanning temperatures from 3500 K to 30,000 K and log g from 0 to 5, where larger temperatures only have high gravities. The MARCS models span from 3500 K to 5500 K, and log g from 0 to 5. All model atmospheres are publicly available online.
Red giants in the updated APOGEE-Kepler catalogue, with estimates of mass, chemical composition, surface gravity and effective temperature, have recently challenged stellar models computed under the ...standard assumption of solar calibrated mixing length. In this work, we critically reanalyse this sample of red giants, adopting our own stellar model calculations. Contrary to previous results, we find that the disagreement between the Teff scale of red giants and models with solar calibrated mixing length disappears when considering our models and the APOGEE-Kepler stars with scaled solar metal distribution. However, a discrepancy shows up when α-enhanced stars are included in the sample. We have found that assuming mass, chemical composition and effective temperature scale of the APOGEE-Kepler catalogue, stellar models generally underpredict the change of temperature of red giants caused by α-element enhancements at fixed Fe/H. A second important conclusion is that the choice of the outer boundary conditions employed in model calculations is critical. Effective temperature differences (metallicity dependent) between models with solar calibrated mixing length and observations appear for some choices of the boundary conditions, but this is not a general result.
ABSTRACT We report on the detection, from observations obtained with the Apache Point Observatory Galactic Evolution Experiment spectroscopic survey, of a metal-poor (Fe/H = −1.3 dex) field giant ...star with an extreme Mg-Al abundance ratio (Mg/Fe = −0.31 dex; Al/Fe = 1.49 dex). Such low Mg/Al ratios are seen only among the second-generation population of globular clusters (GCs) and are not present among Galactic disk field stars. The light-element abundances of this star, 2M16011638-1201525, suggest that it could have been born in a GC. We explore several origin scenarios, studying the orbit of the star in particular to check the probability of its being kinematically related to known GCs. We performed simple orbital integrations assuming the estimated distance of 2M16011638-1201525 and the available six-dimensional phase-space coordinates of 63 GCs, looking for close encounters in the past with a minimum distance approach within the tidal radius of each cluster. We found a very low probability that 2M16011638-1201525 was ejected from most GCs; however, we note that the best progenitor candidate to host this star is GC Centauri (NGC 5139). Our dynamical investigation demonstrates that 2M16011638-1201525 reaches a distance from the Galactic plane and minimum and maximum approaches to the Galactic center of Rmin < 0.62 kpc and Rmax < 7.26 kpc in an eccentric (e ∼ 0.53) and retrograde orbit. Since the extreme chemical anomaly of 2M16011638-1201525 has also been observed in halo field stars, this object could also be considered a halo contaminant, likely to have been ejected into the Milky Way disk from the halo. We conclude that 2M16011638-20152 is also kinematically consistent with the disk but chemically consistent with halo field stars.
We report the first detailed chemical abundance analysis of the exoplanet-hosting M-dwarf stars Kepler-138 and Kepler-186 from the analysis of high-resolution (R ∼ 22,500) H-band spectra from the ...SDSS-IV-APOGEE survey. Chemical abundances of 13 elements-C, O, Na, Mg, Al, Si, K, Ca, Ti, V, Cr, Mn, and Fe-are extracted from the APOGEE spectra of these early M-dwarfs via spectrum syntheses computed with an improved line list that takes into account H2O and FeH lines. This paper demonstrates that APOGEE spectra can be analyzed to determine detailed chemical compositions of M-dwarfs. Both exoplanet-hosting M-dwarfs display modest sub-solar metallicities: Fe/HKepler-138 = −0.09 0.09 dex and Fe/HKepler-186 = −0.08 0.10 dex. The measured metallicities resulting from this high-resolution analysis are found to be higher by ∼0.1-0.2 dex than previous estimates from lower-resolution spectra. The C/O ratios obtained for the two planet-hosting stars are near-solar, with values of 0.55 0.10 for Kepler-138 and 0.52 0.12 for Kepler-186. Kepler-186 exhibits a marginally enhanced Si/Fe ratio.
ABSTRACT
The Milky Way provides an ideal laboratory to test our understanding of galaxy evolution, owing to our ability to observe our Galaxy over fine scales. However, connecting the Galaxy to the ...wider galaxy population remains difficult, due to the challenges posed by our internal perspective and to the different observational techniques employed. Here, we present a sample of galaxies identified as Milky Way analogues on the basis of their stellar masses and bulge-to-total ratios, observed as part of the Mapping Nearby Galaxies at Apache Point Observatory survey. We analyse the galaxies in terms of their stellar kinematics and populations as well as their ionized gas contents. We find our sample to contain generally young stellar populations in their outskirts. However, we find a wide range of stellar ages in their central regions, and we detect central active galactic nucleus-like or composite-like activity in roughly half of the sample galaxies, with the other half consisting of galaxies with central star-forming emission or emission consistent with old stars. We measure gradients in gas metallicity and stellar metallicity that are generally flatter in physical units than those measured for the Milky Way; however, we find far better agreement with the Milky Way when scaling gradients by galaxies’ disc scale lengths. From this, we argue much of the discrepancy in metallicity gradients to be due to the relative compactness of the Milky Way, with differences in observing perspective also likely to be a factor.
Mitochondria are essential, dynamic organelles that respond to a number of stressors with changes in morphology that are linked to several mitochondrial functions, though the mechanisms involved are ...poorly understood. We show that the levels of the regulatory GTPase ARL2 and its GAP, ELMOD2, are specifically increased at mitochondria in immortalized mouse embryo fibroblasts deleted for Mitofusin 2 (MFN2), but not MFN1. Elevated ARL2 and ELMOD2 in MEFs deleted for MFN2 could be reversed by re-introduction of MFN2, but only when the mitochondrial fragmentation in these MEFs was also reversed, demonstrating that reversal of elevated ARL2 and ELMOD2 requires the fusogenic activity of MFN2. Other stressors with links to mitochondrial morphology were investigated and several, including glucose or serum deprivation, also caused increases in ARL2 and ELMOD2. In contrast, a number of pharmacological inhibitors of energy metabolism caused increases in ARL2 without affecting ELMOD2 levels. Together we interpret these data as evidence of two ARL2-sensitive pathways in mitochondria, one affecting ATP levels that is independent of ELMOD2 and the other leading to mitochondrial fusion involving MFN2 that does involve ELMOD2.