The cast-grown monocrystalline-like silicon (mono-like Si) technology has been reactivated recently for the manufacture of high-efficiency solar cells at low cost. In this paper, we have provided a ...progressive research, both experimentally and theoretically, to improve the efficiency of mono-like Si passivated emitter and rear cells (PERCs) through production lines. By employing rear surface passivation of AlOx/triple-SiNx:H layers, the average efficiency has increased to 21.87% with a champion efficiency of 22.32% from the standard mono-like Si PERCs of 21.72% efficiency with AlOx capped with double-SiNx:H layers. We have further optimized the rear electrode contact patterns and introduced the selective emitter (SE) technology, yielding an absolute efficiency improvement of 0.19% and 0.26%, respectively. Integrating all approaches of the triple-SiNx:H, rear contact pattern optimization and SE technology, we have demonstrated that the mono-like Si PERCs can reach the average efficiency of 22.2% with a maximum efficiency of 22.5%, similar to the current Czochralski monocrystalline Si counterparts.
Abstract Engineered nanoparticles have the potential to expand the breadth of pulmonary therapeutics, especially as respiratory vaccines. Notably, cationic nanoparticles have been demonstrated to ...produce superior local immune responses following pulmonary delivery; however, the cellular mechanisms of this increased response remain unknown. To this end, we systematically investigated the cellular response of lung APCS following pulmonary instillation of anionic and cationic charged nanoparticles. While nanoparticles of both surface charges were capable of trafficking to the draining lymph node and were readily internalized by alveolar macrophages, both CD11b and CD103 lung dendritic cell (DC) subtypes preferentially associated with cationic nanoparticles. Instillation of cationic nanoparticles resulted in the upregulation of Ccl2 and Cxc10 , which likely contributes to the recruitment of CD11b DCs to the lung. In total, these cellular mechanisms explain the increased efficacy of cationic formulations as a pulmonary vaccine carrier and provide critical benchmarks in the design of pulmonary vaccine nanoparticles.
Accurate and robust pathological image analysis for colorectal cancer (CRC) diagnosis is time-consuming and knowledge-intensive, but is essential for CRC patients' treatment. The current heavy ...workload of pathologists in clinics/hospitals may easily lead to unconscious misdiagnosis of CRC based on daily image analyses.
Based on a state-of-the-art transfer-learned deep convolutional neural network in artificial intelligence (AI), we proposed a novel patch aggregation strategy for clinic CRC diagnosis using weakly labeled pathological whole-slide image (WSI) patches. This approach was trained and validated using an unprecedented and enormously large number of 170,099 patches, > 14,680 WSIs, from > 9631 subjects that covered diverse and representative clinical cases from multi-independent-sources across China, the USA, and Germany.
Our innovative AI tool consistently and nearly perfectly agreed with (average Kappa statistic 0.896) and even often better than most of the experienced expert pathologists when tested in diagnosing CRC WSIs from multicenters. The average area under the receiver operating characteristics curve (AUC) of AI was greater than that of the pathologists (0.988 vs 0.970) and achieved the best performance among the application of other AI methods to CRC diagnosis. Our AI-generated heatmap highlights the image regions of cancer tissue/cells.
This first-ever generalizable AI system can handle large amounts of WSIs consistently and robustly without potential bias due to fatigue commonly experienced by clinical pathologists. It will drastically alleviate the heavy clinical burden of daily pathology diagnosis and improve the treatment for CRC patients. This tool is generalizable to other cancer diagnosis based on image recognition.
The fruit of Forsythia suspense (Thunb.) Vahl, a well-known Chinese Materia Medica, has been traditionally used in traditional Chinese medicine for the treatment of diabetes and some other diseases, ...but the rational for the usage of this plant is unclear. The aim of this study was to investigate the therapeutic effect and potential mechanism of the fruit of F. suspensa using streptozotocin (STZ)-induced diabetic mice.
Crude methanol extract of F. suspense fruit was fractionated with different solvents and the ethyl acetate fraction (EAF) was selected for in vivo studies based on the in vitro α-amylase and HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl coenzyme A) inhibiting activities. For in vivo study, diabetes mellitus was induced in mice with STZ. Diabetic mice were orally administrated with 50, 100 and 200mg/kg body weight of EAF for 4 weeks. Mouse body weight, blood glucose, glucose tolerance, biochemical parameters and gene expression related to pancreas and liver function were analyzed after EAF administration.
After 4 weeks of EAF intervention, a significant decrease in blood glucose, triglyceride, creatinine total cholesterol, acid phosphatase, alkaline phosphatase, aspartate transaminase, alanine transaminase, and hepatic lipid (triglycerides and cholesterol) content as well as a significant increase in body weight, insulin secretion and glucose tolerance was observed in EAF treated diabetic mice. qRT-PCR analysis revealed that EAF antagonized STZ-induced alteration of the expression of rate-limiting enzymes (glucokinase and phosphorenolpyruvate carboxykinase) in liver and insulin secretion related genes insulin-1, insulin-2 and duodenal homeobox factor-1 in pancreas.
The ethyl acetate extract of Forsythia suspense (Thunb.) Vahl fruit has potency to develop an antihyperglycemic and antihyperlipidemic agent for the treatment of diabetes mellitus via modulation of oxidative stress, the hepatic glucose metabolism and pancreatic insulin secretion.
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Mineral grains and porosity are two common properties of geomaterials. This study focuses on establishing a macroscopic yield criterion for geomaterials having pores and mineral grains at the ...mesoscale which is embedded in a porous matrix. At the microscale, the solid phase is pressure-sensitive and obeys to the Drucker–Prager criterion which considers the asymmetric property between tension and compression. Based on a two-step homogenization procedure and the modified secant method, a macroscopic yield criterion is firstly derived which takes into account the effects of microstructure on the macroscopic mechanical behaviors of the studied material, such as the meso-porosity and mineral grains content at the mesoscale, the micro-porosity and the pressure-sensitive of the solid phase at the microscale. However, it overestimates the material strength in compressive loading by comparing with the Fast Fourier Transform (FFT) based numerical results. Then, this criterion is improved by considering the exact solution in pure hydrostatic loading of a porous medium with a Drucker–Prager matrix. By comparing to the numerical results with different microstructures and to experimental data, the new macroscopic yield criterion is estimated and validated. The main features of the studied geomaterial are well captured by this criterion.
•Plastic yield criteria are derived for geomaterial with a complex microstructure•The effects of meso-grains, meso- and micro-porosities are explicitly considered•The pressure sensitivity of the solid phase at the microscale is taken into account•The criterion is validated by comparing with numerical and experimental results•The present work provides a sound background for various future works.
Accurate, rapid, and comprehensive biodiversity assessments are critical for investigating ecological processes and supporting conservation efforts. Environmental DNA (eDNA) surveys show promise as a ...way to effectively characterize fine-scale patterns of community composition. We tested whether a single PCR survey of eDNA in seawater using a broad metazoan primer could identify differences in community composition between five adjacent habitats at 19 sites across a tropical Caribbean bay in Panama. We paired this effort with visual fish surveys to compare methods for a conspicuous taxonomic group. eDNA revealed a tremendous diversity of animals (8,586 operational taxonomic units), including many small taxa that would be undetected in traditional in situ surveys. Fish comprised only 0.07% of the taxa detected by a broad COI primer, yet included 43 species not observed in the visual survey. eDNA revealed significant differences in fish and invertebrate community composition across adjacent habitats and areas of the bay driven in part by taxa known to be habitat-specialists or tolerant to wave action. Our results demonstrate the ability of broad eDNA surveys to identify biodiversity patterns in the ocean.
Because apoptosis of infected cells can limit virus production and spread, some viruses have co-opted prosurvival genes from the host. This includes the Epstein-Barr virus (EBV) gene BHRF1, a homolog ...of human Bcl-2 proteins that block apoptosis and are associated with cancer. Computational design and experimental optimization were used to generate a novel protein called BINDI that binds BHRF1 with picomolar affinity. BINDI recognizes the hydrophobic cleft of BHRF1 in a manner similar to other Bcl-2 protein interactions but makes many additional contacts to achieve exceptional affinity and specificity. BINDI induces apoptosis in EBV-infected cancer lines, and when delivered with an antibody-targeted intracellular delivery carrier, BINDI suppressed tumor growth and extended survival in a xenograft disease model of EBV-positive human lymphoma. High-specificity-designed proteins that selectively kill target cells may provide an advantage over the toxic compounds used in current generation antibody-drug conjugates.
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•A specific inhibitor of an EBV prosurvival Bcl-2 protein was computationally designed•The likelihood of designed proteins correctly folding correlates with activity•The designed inhibitor can initiate apoptosis in EBV-infected cancer cells•Intracellular delivery with a micelle carrier suppresses xenograft tumor growth
A novel computationally designed protein, called BINDI, binds to Epstein-Barr virus Bcl-2 protein with picomolar affinity. BINDI initiates apoptosis in EBV-infected cancer lines, can suppress tumor growth, and extends survival in a xenograft disease model of EBV-positive human lymphoma.
•The overall phosphorylation of sarcoplasmic proteins was altered by adding inhibitors.•Meat color stability is significantly affected by protein phosphorylation.•Protein phosphorylation regulates ...glycolysis in postmortem muscle.•Phosphorylation regulates meat color by regulating glycolysis and the redox state of myoglobin.
The influence of protein phosphorylation on meat color stability was investigated in this study. Phosphatase and protein kinase inhibitors were added to minced ovine Longissimus thoracis et lumborum (LTL) muscle to manipulate the global phosphorylation of sarcoplasmic proteins. The data obtained show that the rate and extent of pH decline, along with lactate accumulation in postmortem muscle, were related to protein phosphorylation. Analysis of meat color and the relative content of myoglobin redox forms revealed that meat color stability was inversely related to the phosphorylation of sarcoplasmic proteins. Thus, this study suggests that protein phosphorylation may be involved in meat color development by regulating glycolysis and the redox stability of myoglobin.
Prime editing enables search-and-replace genome editing but is limited by low editing efficiency. We present a high-throughput approach, the Peptide Self-Editing sequencing assay (PepSEq), to measure ...how fusion of 12,000 85-amino acid peptides influences prime editing efficiency. We show that peptide fusion can enhance prime editing, prime-enhancing peptides combine productively, and a top dual peptide-prime editor increases prime editing significantly in multiple cell lines across dozens of target sites. Top prime-enhancing peptides function by increasing translation efficiency and serve as broadly useful tools to improve prime editing efficiency.
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