Were magnetic materials useful in cancer therapy? Baghban, Roghayyeh; Afarid, Mehrdad; Soleymani, Jafar ...
Biomedicine & pharmacotherapy,
December 2021, 2021-Dec, 2021-12-00, 20211201, 2021-12-01, Letnik:
144
Journal Article
Recenzirano
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Cancer is one of the major challenges fronting the biomedical basic researches in our time. The study and development of effective therapeutic strategies for cancer therapy are vital. Among the many ...probable core constituents of nanoparticles, magnetite-based nanoparticles have been widely studied for cancer therapy owing to their inherent magnetic features, multifunctional design, biodegradable and biocompatible properties. Magnetic nanoparticles have been also designed for utilizing as contrast enhancer agents for magnetic resonance imaging, drug delivery systems, and most recently as a therapeutic element in inducing cellular death in tumor ablation therapies. This review aimed to provide an overview of the various applications of magnetic nanoparticles and recent achievements in developing these advanced materials for cancer therapy.
A simple and sensitive dual-emission ratiometric fluorescent probe was developed using zeolitic imidazolate framework 8 (ZIF8) and L-ascorbic acid Au-doped dopamine nanoparticles (ZIF8/L-ASC-AuNP/DA ...NP) for the determination of pioglitazone (Pio), an oral hypoglycemic agent and insulin sensitizer, in real samples. The prepared system was based on the Pio-enhanced dual-emission intensity of ZIF8/L-ASC-AuNP/DA NP. The potential impact of various parameters on the system’s emission intensity was tested. According to the findings, there is a strong linear correlation between the system's turn-on fluorescence intensity and Pio concentrations in the range 0.3 nM to 30.0 μM. The obtained value for the limit of detection (LOD) was 0.14 nM. In addition, the intra- and inter-day accuracy of the nanoprobe was studied and the findings revealed satisfactory precision and accuracy of the system. The short-term and freeze-thaw stability of Pio in plasma samples was evaluated and the results indicated the high stability of the developed nanoprobe under the test conditions. Pio was accurately detected in human plasma samples under ideal conditions with analytical recoveries in the range 86.0 − 109.3%. The results showed that the devised probe may be employed as an easy, sensitive, and precise approach for detecting Pio in real samples.
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A novel ratiometric fluorescent sensor was fabricated for the fast and facile determination of cyclosporine A (CsA). Due to the narrow therapeutic index of CsA, its desired therapeutic effects are ...evident within a limited range of blood concentration, indicating the fundamental role of therapeutic drug monitoring in CsA pharmacological response. In this study, a two-photon fluorescence probe based on the zeolitic imidazolate framework (ZIF-8) and norepinephrine-capped silver nanoparticles (AgNPs@NE) was employed for the quantification of the CsA in human plasma samples. In the presence of CsA, the fluorescent emission intensity of ZIF-8-AgNPs@NE was quenched. Under the optimum conditions, the proposed probe determines CsA in plasma samples in two linear ranges of 0.01 to 0.5 μg mL
−1
and 0.5 to 10 μg mL
−1
. The developed probe demonstrates the advantages of a facile and fast platform with limit of detection as low as 0.007 μg mL
−1
. At last, this method was applied to find CsA concentration in four patients receiving oral CsA regimen which indicates it as a promising method for on-site detection applications.
Graphical abstract
The technology of ink-jet printing has been an appealing alternative to traditional photolithography for fabricating flexible and elastic circuits, having low-cost and ease of control. In this work, ...a novel, unique and sensitive immunoassay designed based on application of silver nanoparticles-reduced graphene oxide nanocomposite (Ag/RGO) ink for fabrication of paper based immunosensor, Cysteamine coped gold nanoparticles (CysA/Au NPs) as a signal amplification element for determination of CA15-3 protein. Fully electrochemically methodology was applied to prepare a new transducer based on the Ag/RGO surface which provides high surface area for CA15-3 antibody immobilization in high amount. Under optimized experimental conditions, the Ag-RGO/CysA-Au NPs based immunosensor shows good specificity and sensitivity. The obtained LLOQ of immunosensor for CA 15-3 biomarker was 15 U/mL as well as calibration curve was linear in the range of 15–125 U/mL. The technique was successfully employed assay of CA15-3 biomarker in unprocessed human plasma samples.
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•A novel immunoassay for detection of breast cancer specific protein CA 15-3 was successfully developed.•New platform based on conductive ink technology for sensitive voltammetric detection of CA 15-3 was designed.•The amperometric response of the CA 15-3 exhibited suitable results compared with the other type of bioassays.
According to the reports of the World Health Organization and the International Agency for Research on Cancer, cancer is the second leading cause of human death worldwide. However, early-stage ...detection of cancers can efficiently enhance the chance of therapy and saving lives. Metabolomics strategies apply a variety of approaches to discover new potential diagnoses, prognoses, and/or therapeutic biomarkers of various diseases. Metabolomics aims to identify and measure different low-molecular-weight biomolecules in physiological environments. In these studies, special metabolites are extracted from biological samples and identified using analytical techniques. Afterward, using data processing programs discovering significantly associated biomarkers is pursued. In the present review, we aimed to discuss recently reported analytical approaches on the metabolomics studies of gastrointestinal cancers including gastric, colorectal, and esophageal cancers. The gas- and liquid-chromatography with different detectors have been shown that are the main analytical techniques and for metabolites quantification, nuclear magnetic resonance has been utilized as a master method.
Silibinin is effective in significantly inhibiting the growth of cancer cells which shown significant anti-neoplastic effects in a variety of in vitro and in vivo cancer models, including skin, ...breast, lung, colon, bladder, prostate and kidney carcinomas. So, development of a new method to its biomedical analysis in clinical samples in highly demanded. In this study, an innovative electroanalysis method for the accurate, sensitive and rapid recognition of silibinin in human plasma samples was proposed and validated. The sensing platform was designed using silver nanoparticles (AgNPs) dispersed on the polymeric layer of β-cyclodextrin (β-CD). AgNPs with cubic shape providing a large effective surface area for β-CD electropolymerization. So, a layer with high electron conductivity boosting the detection electrochemical signals. Also, poly(β-CD) providing an efficient substrate with cavities to interact with silibinin and its oxidation. Differential pulse voltammetry technique was conducted to measure silibinin concentration in human real samples. Under optimized conditions, proposed sensor indicated linear relationship between the anodic peak current and concentration of silibinin in the range of 0.0103–10.3 µM on the standard and human plasma samples. Based on obtained results, proposed sensor is an efficient platform to efficient therapy of cancer based on recognition of silibinin in clinical samples.
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Regulation and modulation of PTEN activity Naderali, Elahe; Khaki, Amir Afshin; Rad, Jafar Soleymani ...
Molecular biology reports,
12/2018, Letnik:
45, Številka:
6
Journal Article
Recenzirano
PTEN (Phosphatase and tensin homolog deleted on chromosome ten) is a tumor suppressor that is frequently mutated in most human cancers. PTEN is a lipid and protein phosphatase that antagonizes ...PI3K/AKT pathway through lipid phosphatase activity at the plasma membrane. More recent studies showed that, in addition to the putative role of PTEN as a PI(3,4,5)P3 3-phosphatase, it is a PI(3,4)P2 3-phosphatase during stimulation of class I PI3K signaling pathway by growth factor. Although PTEN tumor suppressor function via it’s lipid phosphatase activity occurs primarily in the plasma membrane, it can also be found in the nucleus, in cytoplasmic organelles and extracellular space. PTEN has also shown phosphatase independent functions in the nucleus. PTEN can exit from the cell through exosomal export or secretion and has a tumor suppressor function in adjacent cells. PTEN has a critical role in growth, the cell cycle, protein synthesis, survival, DNA repair and migration. Understanding the regulation of PTEN function, activity, stability, localization and its dysregulation outcomes and also the intracellular and extracellular role of PTEN and paracrine role of PTEN-L in tumor cells as an exogenous therapeutic agent can help to improve clinical conceptualization and treatment of cancer.
In this study, a novel nano-composite based on poly‑taurine/beta‑cyclodextrin and graphene quantum dot used for the modification of gold electrode by simple electrodeposition approach. The prepared ...interface was applied for the sensing of doxorubicin hydrochloride (DOX) in human plasma samples without any pretreatment procedure. The synergistic influence of poly-taurine, beta-cyclodextrin, and graphene quantum dot not only remarkably enhances electroactivity, but also speeds up the electron shift between DOX and the electrode substrates. Cyclic voltammetry results indicated that the DOX is oxidized via two electrons and two protons process at the optimum pH = 4 using poly-taurine/beta-cyclodextrin and graphene quantum dots thin film modified gold electrode. More momentously electrostatic repulsion takes place between the prepared polymeric film and DOX resulting in the signal amplification on oxidation of this drug and lowering its overpotential and thereby sensitive and selective detection of DOX in real samples. The apparent electron transfer rate constant and transfer coefficients were determined by cyclic voltammetry. Using differential-pulse voltammetric (DPV) technique sensitive determination of DOX in human plasma samples was performed with wide dynamic range and good limit of detection.
•The use of beta cyclodextrin as a bioactive monosaccharide on the detection of DOX in human real samples was developed.•A novel bioanalytical approach based on P(TA-β-CD)-GQDs was used for quantification of DOX in human biofluids was investigated.•Real time bioanalysis of DOX for a direct evaluation of chemotherapy efficiency is applicable.
Tacrolimus (Tac) is a well-documented immunosuppressive agent for the prevention of graft-vs-host diseases in several types of organ transplants. The narrow therapeutic window and the ...individual-variable pharmacokinetics of Tac demonstrate the importance of regular therapeutic drug monitoring (TDM) as an imperative concept for its oral medication regimens. A simple, one-step, selective, and sensitive colorimetric platform is fabricated for the determination of Tac by surface modification of the silver nanoparticles (AgNPs) via norepinephrine (NE) molecules. The attachment of NE and Tac induces the aggregation of the AgNPs, which is observed by color distinction (yellow to brown) and a noteworthy shifting of the absorption peak in the visible region. The fabricated nanoprobe can detect Tac concentrations in plasma samples in two linear ranges from 2 ng/mL to 70 ng/mL and 70 ng/mL to 1000 ng/mL with R2 > 0.99. The limit of detection (LOD) was calculated as low as 0.1 ng/mL. The developed method was applied for the determination of Tac in patient’s plasma samples under Tac medication therapy.
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