Many lines of evidence link antimicrobial-resistant human infections to foodborne pathogens of animal origin. Types of evidence reviewed include: (1) direct epidemiologic studies; (2) temporal ...evidence; (3) additional circumstantial evidence; (4) trends in antimicrobial resistance among Salmonella isolates; and (5) trends in antimicrobial resistance among other pathogens, such as Campylobacter jejuni. Commensal microorganisms in animals and humans may contribute to antimicrobial resistance among pathogens that cause disease among humans. For instance, enterococci of food-animal origin, particularly strains that are vancomycin resistant, have been linked to strains found in the human gastrointestinal tract. The latent period between the introduction of a given antimicrobial and emergence of resistance varies considerably, but once the prevalence in a population reaches a certain level, control becomes extremely difficult.
Vaccination against coliform mastitis has become part of mastitis control programs in the past 3 decades, as a means of reducing the severity of clinical mastitis. Our study objective was to evaluate ...the effect of 2 commercially available vaccines on clinical, behavioral, and antibody response following Escherichia coli intramammary challenge in cows near peak lactation. Cows (n = 12 per group) were vaccinated with vaccine 1 (V1) or vaccine 2 (V2) at dry-off, 21 d pre-calving, and 14 d post-calving. Twelve cows served as unvaccinated controls (CTL). Cows were challenged with E. coli in a rear quarter at approximately 100 d in milk. Milk samples were collected pre- and post-challenge to enumerate E. coli and determine somatic cell count. Serum was collected before each vaccination and at d 0, 1, 2, 3, 6, 30, and 60 relative to challenge, to study antibody response. Milk IgA and tumor necrosis factor-α concentrations were determined in whey. Vaginal temperature, cow activity, and milk yield and components were monitored post-challenge. Bacterial count, somatic cell score, milk yield and component decline, vaginal temperature, activity measures, and antibody and cytokine response were analyzed for treatment differences. The effects of parity, breed, and a repeated measure of time were also tested. Seven cows had to be removed from the study post-challenge for antibiotic treatment (CTL and V1, n = 3 each; V2, n = 1), 2 of which were euthanized (both CTL). Vaccinated cows exhibited fever (vaginal temperature ≥39.4°C) 3 h earlier than CTL cows, but we found no differences between treatments for bacterial count, somatic cell score, or milk yield reduction. Vaccinated cows spent more time lying per rest bout 2 d post-challenge, but total daily lying time was not different from CTL cows during the 7 d post-challenge. The vaccines differed in antibody response: V1 cows had greater serum IgG1 and IgG2 post-challenge. A parity effect was also evident: primiparous cows had lower bacterial counts, somatic cell score and a smaller milk yield decline than multiparous cows, but also had lower antibody production. Immunization with either J5 bacterin did not reduce clinical signs of mastitis in cows challenged at 100 d in milk, demonstrating that the effects of J5 vaccination had diminished at peak lactation.
About 5% of patients admitted to acute-care hospitals acquire nosocomial infections. A variety of factors contribute: increasing age of patients; availability, for treatment of formerly untreatable ...diseases, of extensive surgical and intensive medical therapies; and frequent use of antimicrobial drugs capable of selecting a resistant microbial flora. Nosocomial infections due to resistant organisms have been a problem ever since infections due to penicillinase-producing Staphylococcus aureus were noted within a few years of the introduction of penicillin. By the 1960s aerobic Gram-negative bacilli had assumed increasing importance as nosocomial pathogens, and many strains were resistant to available antimicrobials. During the 1980s the principal organisms causing nosocomial bloodstream infections were coagulase-negative staphylococci, aerobic Gram-negative bacilli, S. aureus, Candida spp., and Enterococcus spp. Coagulase-negative staphylococci and S. aureus are often methicillin-resistant, requiring parenteral use of vancomycin. Prevalence of vancomycin resistance among enterococcal isolates from patients in intensive care units has increased, likely due to increased use of this drug. Plasmid-mediated gentamicin resistance in up to 50% of enterococcal isolates, along with enhanced penicillin resistance in some strains, leaves few therapeutic options. The emergence of Enterobacteriaceae with chromosomal or plasmid-encoded extended spectrum β-lactamases presents a world-wide problem of resistance to third generation cephalosporins. Control of these infections rests on (i) monitoring infections with such resistant organisms in an ongoing fashion, (ii) prompt institution of barrier precautions when infected or colonized patients are identified, and (iii) appropriate use of antimicrobials through implementation of antibiotic control programs.
To characterize acute bacterial meningitis in adults, we reviewed the charts of all persons 16 years of age or older in whom acute bacterial meningitis was diagnosed at Massachusetts General Hospital ...from 1962 through 1988. We included patients who were admitted after initial treatment at other hospitals.
During the 27-year period, 445 adults were treated for 493 episodes of acute bacterial meningitis, of which 197 (40 percent) were nosocomial. Gram-negative bacilli (other than Haemophilus influenzae) caused 33 percent of the nosocomial episodes but only 3 percent of the community-acquired episodes. In the 296 episodes of community-acquired meningitis, the most common pathogens were Streptococcus pneumoniae (37 percent), Neisseria meningitidis (13 percent), and Listeria monocytogenes (10 percent); these organisms accounted for only 8 percent of the nosocomial episodes. Only 19 of the 493 episodes of meningitis (4 percent) were due to H. influenzae. Nine percent of all patients had recurrent meningitis; many had a cerebrospinal fluid leak. Seizures occurred in 23 percent of patients with community-acquired meningitis, and 28 percent had focal central nervous system findings. Risk factors for death among those with single episodes of community-acquired meningitis included older age (> or = 60 years), obtunded mental state on admission, and seizures within the first 24 hours. Among those with single episodes, the in-hospital mortality rate was 25 percent for community-acquired and 35 percent for nosocomial meningitis. The overall case fatality rate was 25 percent and did not vary significantly over the 27 years.
In our large urban hospital, a major proportion of cases of acute bacterial meningitis in adults were nosocomial. Recurrent episodes of meningitis were frequent. The overall mortality rate remained high.
Knowledge of the low-lying monopole strength in C12—the Hoyle state in particular—is crucial for our understanding of both the astrophysically important 3α reaction and of α-particle clustering. ...Multiple theoretical models have predicted a breathing mode of the Hoyle State at Ex≈9 MeV, corresponding to a radial in-phase oscillation of the underlying α clusters. The C12(α,α′)C12 and C14(p,t)C12 reactions were employed to populate states in 12C in order to search for this predicted breathing mode. A self-consistent, simultaneous analysis of the inclusive spectra with R-matrix lineshapes, together with angular distributions of charged-particle decay, yielded clear evidence for excess monopole strength at Ex≈9 MeV which is highly collective. Reproduction of the experimentally observed inclusive yields using a fit, with consistent population ratios for the various broad states, required an additional source of monopole strength. The interpretation of this additional monopole resonance as the breathing-mode excitation of the Hoyle state would provide evidence supporting a D3h symmetry for the Hoyle state itself. The excess monopole strength may complicate analysis of the properties of the Hoyle state, modifying the temperature dependence of the 3α rate at T9≳2 and ultimately, the predicted nucleosynthesis in explosive stars.
Arsenic Mobility and Groundwater Extraction in Bangladesh Harvey, Charles F.; Swartz, Christopher H.; Badruzzaman, A. B. M. ...
Science (American Association for the Advancement of Science),
11/2002, Letnik:
298, Številka:
5598
Journal Article
Recenzirano
High levels of arsenic in well water are causing widespread poisoning in Bangladesh. In a typical aquifer in southern Bangladesh, chemical data imply that arsenic mobilization is associated with ...recent inflow of carbon. High concentrations of radiocarbon-young methane indicate that young carbon has driven recent biogeochemical processes, and irrigation pumping is sufficient to have drawn water to the depth where dissolved arsenic is at a maximum. The results of field injection of molasses, nitrate, and low-arsenic water show that organic carbon or its degradation products may quickly mobilize arsenic, oxidants may lower arsenic concentrations, and sorption of arsenic is limited by saturation of aquifer materials.
Heart rate (HR) and motion sensors represent promising tools for physical activity (PA) assessment, as each provides an estimate of energy expenditure (EE). Although each has inherent limitations, ...the simultaneous use of HR and motion sensors may increase the accuracy of EE estimates. The primary purpose of this study was to establish the accuracy of predicting EE from the simultaneous HR-motion sensor technique. In addition, the accuracy of EE estimated by the simultaneous HR-motion sensor technique was compared to that of HR and motion sensors used independently.
Thirty participants (16 men: age, 33.1 +/- 12.2 yr; BMI, 26.1 +/- 0.7 kg.m(-2); and 14 women: age, 31.9 +/- 13.1 yr; BMI, 27.2 +/- 1.1 kg.m(-2) (mean +/- SD)) performed arm and leg work in the laboratory for the purpose of developing individualized HR-VO2 regression equations. Participants then performed physical tasks in a field setting for 15 min each. CSA accelerometers placed on the arm and leg were to discriminate between upper and lower body movement, and HR was then used to predict EE (METs) from the corresponding arm or leg laboratory regression equation. A hip-mounted CSA accelerometer and Yamax pedometer were also used to predict EE. Predicted values (METs) were compared to measured values (METs), obtained via a portable metabolic measurement system (Cosmed K4b(2)).
The Yamax pedometer and the CSA accelerometer on the hip significantly underestimated the energy cost of selected physical activities, whereas HR alone significantly overestimated the energy cost of selected physical activities. The simultaneous HR-motion sensor technique showed the strongest relationship with VO(2) (R(2) = 0.81) and did not significantly over- or underpredict the energy cost (P = 0.341).
The simultaneous HR-motion sensor technique is a good predictor of EE during selected lifestyle activities, and allows researchers to more accurately quantify free-living PA.
PDF
