To investigate the association between eating behaviours (eating speed and energy intake of main meals) and overweight in pre-school children.
Cross-sectional study. Data consisted of measurements ...(height and weight), questionnaire information (eating behaviours of eating speed and overeating) and on-site observation data (meal duration and energy intake of main meals).
Seven kindergartens in Beijing, China.
Pre-school children (n 1138; age range 3·1-6·7 years old) from seven kindergartens participated in the study.
The multivariate-adjusted odds ratio of overweight in participants with parent-reported 'more than needed food intake' was 3·02 (95 % CI 2·06, 4·44) compared with the 'medium food intake' participants, and higher eating speed was associated with childhood overweight. For the two observed eating behaviours, each 418·7 kJ (100 kcal) increase of lunch energy intake significantly increased the likelihood for overweight by a factor of 1·445, and each 5-min increase in meal duration significantly decreased the likelihood for overweight by a factor of 0·861. Increased portions of rice and cooked dishes were significantly associated with overweight status (OR = 2·274; 95 % CI 1·360, 3·804 and OR = 1·378; 95 % CI 1·010, 1·881, respectively).
Eating speed and excess energy intake of main meals are associated with overweight in pre-school children.
Thermophilic proteins have various practical applications in theoretical research and in industry. In recent years, the demand for thermophilic proteins on an industrial scale has been increasing; ...therefore, the engineering of thermophilic proteins has become a hot direction in the field of protein engineering. However, the exact mechanism of thermostability of proteins is not yet known, for engineering thermophilic proteins knowing the basis of thermostability is necessary. In order to understand the basis of the thermostability in proteins, we have made a statistical analysis of the sequences, secondary structures, hydrogen bonds, salt bridges, DHA (Donor–Hydrogen–Accepter) angles, and bond lengths of ten pairs of thermophilic proteins and their non-thermophilic orthologous. Our findings suggest that polar amino acids contribute to thermostability in proteins by forming hydrogen bonds and salt bridges which provide resistance against protein denaturation. Short bond length and a wider DHA angle provide greater bond stability in thermophilic proteins. Moreover, the increased frequency of aromatic amino acids in thermophilic proteins contributes to thermal stability by forming more aromatic interactions. Additionally, the coil, helix, and loop in the secondary structure also contribute to thermostability.
Escherichia coli (E. coli) is one of the important causative pathogens of neonatal invasive infection. The epidemiological and clinical profile of invasive E. coli infection in Chinese newborns is ...not well characterized.
Ninety-four infants with invasive E. coli infection were categorized into E. coli early onset disease (EOD) group (onset ≤72 h after birth) (n = 46) and E. coli late onset disease (LOD) group (onset > 72 h) (n = 48). We compared and analyzed the clinical characteristics and drug sensitivity profile of early-onset and late-onset E. coli invasive infection in neonates.
The incidence of E. coli-EOD and E.coli-LOD was 0.45/1000 live births (LBs) and 0.47/1000 LBs, respectively. The incidence of gestational diabetes mellitus, perinatal fever, urinary tract infection, chorioamnionitis, and positive E. coli culture among mothers in the E. coli-EOD group were significantly higher than that in E. coli-LOD group. The incidence of premature birth, low-birth-weight, nosocomial infection, and hospitalization time were significantly higher in the E. coli-LOD group. The main disease in E. coli-EOD group was pneumonia (main clinical manifestation: dyspnea). The main disease in E. coli-LOD group was sepsis (main clinical manifestation: fever). The sensitivity rates of E. coli strains to ampicillin and piperacillin were low (25.00-28.79%); sensitivity to cephalosporins was also low except ceftazidime (lowest sensitivity rate: 57.14%). Sensitivity to compound preparations containing β-lactamase inhibitors was high, even for extended spectrum β-lactamase-positive strains (nearly 100%).
E. coli is an important cause of invasive infection of newborns in Xiamen, China. E. coli-EOD was largely attributable to perinatal factors, while E. coli-LOD was largely related to nosocomial infection. Compound preparations containing β-lactamase inhibitor or carbapenem antibiotics should be preferred for neonatal invasive infection by E. coli.
Site-saturation mutagenesis is a powerful tool for protein optimization due to its efficiency and simplicity. A degenerate codon NNN or NNS (K) is often used to encode the 20 standard amino acids, ...but this will produce redundant codons and cause uneven distribution of amino acids in the constructed library. Here we present a novel "small-intelligent" strategy to construct mutagenesis libraries that have a minimal gene library size without inherent amino acid biases, stop codons, or rare codons of
by coupling well-designed combinatorial degenerate primers with suitable PCR-based mutagenesis methods. The designed primer mixture contains exactly one codon per amino acid and thus allows the construction of small-intelligent mutagenesis libraries with one gene per protein. In addition, the software tool DC-Analyzer was developed to assist in primer design according to the user-defined randomization scheme for library construction. This small-intelligent strategy was successfully applied to the randomization of halohydrin dehalogenases with one or two randomized sites. With the help of DC-Analyzer, the strategy was proven to be as simple as NNS randomization and could serve as a general tool to efficiently randomize target genes at positions of interest.
Bacteriophages, which are tremendously important to the ecology and evolution of bacteria, play a key role in the development of genetic engineering. Bacteriophage virion proteins are essential ...materials of the infectious viral particles and in charge of several of biological functions. The correct identification of bacteriophage virion proteins is of great importance for understanding both life at the molecular level and genetic evolution. However, few computational methods are available for identifying bacteriophage virion proteins. In this paper, we proposed a new method to predict bacteriophage virion proteins using a Multinomial Naïve Bayes classification model based on discrete feature generated from the g-gap feature tree. The accuracy of the proposed model reaches 98.37% with MCC of 96.27% in 10-fold cross-validation. This result suggests that the proposed method can be a useful approach in identifying bacteriophage virion proteins from sequence information. For the convenience of experimental scientists, a web server (PhagePred) that implements the proposed predictor is available, which can be freely accessed on the Internet.
Introduction
The aim was to investigate the effect of collagen sponges (CS) as a delivery device for osteoprotegerin (OPG)/bone morphogenetic protein 2 (BMP-2) and support matrix on the tendon-bone ...healing after anterior crusicate ligament (ACL) reconstruction in modeled rabbits.
Materials and Methods
Sixty New Zealand white rabbits were randomly divided into four groups based on treatments they received at the tendon-bone interface after left knee ACL reconstruction: the control group, OPG/BMP-2, CS, and OPG/BMP-2/CS combination. At 4, 8 and 12 weeks post-surgery, five rabbits from each group were euthanized to examine the tendon-bone healing. Levels of OPG and BMP-2 in synovial fluid, the bone tunnel enlargement value, the histomorphological typing of tendon-bone interface, and the bone tunnel area of the tendon-bone interface were compared among different treatments.
Results
The OPG/BMP-2/CS combination treatment group had the highest levels of OPG and BMP-2 in synovial fluid (both
P
< 0.05), the greatest number of Sharpey-like collagen fibers at all test points (
P
< 0.05), the most fibrocartilage enthesis on week 12, the greatest bone tunnel area (
P
< 0.05), and the greatest decrease in bone tunnel enlargement on week 12 (
P
< 0.05). Histomorphological typing of tendon-bone interface of all groups showed changes varying from tendon-bone separation to firm healing, and the change was most significant in the OPG/BMP-2/CS combination treatment group.
Conclusion
CS treatment alone serves as a fixing support, and CS combining with growth factors OPG/BMP-2 ensures slow and stable release of OPG/BMP-2, significantly improves the tendon-bone healing in the rabbit ACL model.
Eradicating poverty and improving human well-being are pivotal objectives for achieving global sustainable development. Sustainable agriculture, as a key domain, plays a crucial role in addressing ...poverty. However, to date, there remains insufficient research on the specific impact of agricultural green development on poverty. To bridge this gap, we utilize panel data from 273 prefecture-level cities in China from 2006 to 2022 to explore how agricultural green development affects poverty based on constructing a regional multidimensional poverty index and an index of green agriculture. Our study reveals that agricultural green development effectively reduces poverty, particularly in regions of deep poverty and the eastern part of China. Further mediating analysis indicates the alleviation of poverty by agricultural green development through the infrastructure, the industrial structure, and the green technology innovation effect. Our findings offer valuable insights for informing policies on agricultural green development and poverty reduction, as well as for improving government resource allocation and strengthening resilience in impoverished areas. By deepening our understanding of the link between green agriculture and poverty, this research significantly contributes to global agricultural sustainability and expedites poverty eradication worldwide.
As the emergence of the connected and autonomous vehicles (CAVs), the platooning technology is believed to play a key role in the future intelligent transportation system. However, current studies ...mainly focus on the beneficial sides of CAV platoons, and less attention is given to their negative effects. This study develops a mixed equilibrium model for CAV platoons and human-driven vehicles (HDVs), which consider both the positive and negative sides of CAV platooning. On the positive side, CAV platoons are assumed to follow user equilibrium (UE) route choice for their information advantages, while HDVs to follow stochastic user equilibrium (SUE). CAV platoons are also presumed to improve the road capacity. On the negative side, the speed of CAV platoons is slower than that of HDVs for safety stakes, which will impede the latter to overtake. The HDVs is split up into overtaking and nonovertaking flows with different speeds. Furthermore, the model is built up as a mixed UE-SUE equilibrium problem and reformulated as a nonlinear complementarity problem. In addition, an optimal path platooning method is proposed to reduce the negative effects, by integrating travel costs of both CAV platoons and HDVs into its objective function. Numerical results show that the introduction of CAV platoons may increase the travel cost at the initial stage, and the proposed method can effectively reduce the platooning disturbance, thus helps promoting the wider applications of CAV platoons.
Thermophilic proteins have important application value in biotechnology and industrial processes. The correct identification of thermophilic proteins provides important information for the ...application of these proteins in engineering. The identification method of thermophilic proteins based on biochemistry is laborious, time-consuming, and high cost. Therefore, there is an urgent need for a fast and accurate method to identify thermophilic proteins. Considering this urgency, we constructed a reliable benchmark dataset containing 1,368 thermophilic and 1,443 non-thermophilic proteins. A multi-layer perceptron (MLP) model based on a multi-feature fusion strategy was proposed to discriminate thermophilic proteins from non-thermophilic proteins. On independent data set, the proposed model could achieve an accuracy of 96.26%, which demonstrates that the model has a good application prospect. In order to use the model conveniently, a user-friendly software package called iThermo was established and can be freely accessed at http://lin-group.cn/server/iThermo/index.html. The high accuracy of the model and the practicability of the developed software package indicate that this study can accelerate the discovery and engineering application of thermally stable proteins.
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