As an economically important fish,
Opsariichthys bidens
has obvious sexual dimorphism and strong reproductive capacity, but no epigenetics study can well explain its phenotypic variations. In recent ...years, many microRNAs involved in the regulation of reproductive development have been explored. In this study, the small RNA libraries of
O. bidens
on the testis and ovary were constructed and sequenced. A total of 295 known miRNAs were obtained and 100 novel miRNAs were predicted. By comparing testis and ovary libraries, 115 differentially expressed (DE) miRNAs were selected, of which 53 were up-regulated and 62 were down-regulated. A total of 64 GO items (padj < 0.01) and 206 KEGG pathways (padj < 0.01) were enriched in the target gene of miRNA. After that, the expression levels of nine DE miRNAs, including let-7a, miR-146b, miR-18c, miR-202-5p, miR-135c, miR-9-5p, miR-34c-3p, miR-460-5p and miR-338 were verified by qRT-PCR. Furthermore, bidirectional prediction of DE miRNAs and sex-related genes was carried out and the targeting correlation between miR-9-5p and
nanos1
was verified by Dual-Luciferase reporter assay. Our findings identified the differentially expressed miRNA and paved the way to new possibilities for the follow-up study on the mechanism of miRNA-mRNA interaction in the gonads of
O. bidens
.
As a small farmed fish, Opsariichthys bidens shows sex phenotypic difference between male and female individuals, but the molecular mechanism of gonadal development is still unclear. This study ...performed transcriptome sequencing on the adult testis and ovary of O. bidens to identify sex-related genes. A total of 50,321 unigenes were obtained from six libraries of gonadal tissues, with a total length of 69,070,743 bp and an average length of 1372 bp. Moreover, 29,889, 24,874, 14,564, 18,519, 27,195 and 22,558 unigenes were annotated in the non-redundant protein database, Swissprot, Kyoto Encyclopedia of Genes and Genomes (KEGG), eukaryotic orthologous group (KOG), evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG) and Gene Ontology (GO) databases, respectively. By comparing testis and ovary libraries, 12,609 differentially expressed genes were identified, of which 5975 were up-regulated in the testis and 6634 were up-regulated in the ovary. Enrichment analysis of differentially expressed genes resulted in 3119 enriched GO items (padj<0.01) and 47 enriched KEGG pathways (padj<0.01). Subsequently, the expression levels of nine sex-related genes, including dazl, dnd, nanog, piwi, vasa, zp4, amh, cyp19a and dmrt1 were confirmed by qRT-PCR. Furthermore, molecular cloning and in situ hybridization demonstrated that both cyp19a and vasa were conserved in O. bidens. In addition, cyp19a was mainly expressed in early developmental stages of oocytes in the ovary but not in the testis. While vasa was present in both testis and ovary, and was highly expressed in the early stages of gametogenesis in gonads. These findings provide basic support for further research on the function of sex-related genes and the molecular mechanisms of sex determination in O. bidens.
•Transcriptome sequencing on the adult gonads of O. bidens was performed and analyzed.•Sex-related genes dazl, dnd, nanog, piwi, vasa, zp4, amh, cyp19a and dmrt1 were identified in O. bidens.•Cyp19a and vasa were specifically expressed in gonads among fish and their sub-cellular distributions were revealed in O. bidens.
PCR-based DNA amplification has been one of the major methods in aquaculture research for decades, although its use outside the modern laboratory environment is limited due to the relatively complex ...methods and high costs. To this end, we investigated a swabbing and disc protocol for the collection of DNA samples from fish which could extract DNA from fish skin mucus by a non-invasion technique costing only $0.02 (USD) and requiring less than 30 seconds. The disc method that we chose could use the cheap filter paper to extract DNA from above 10
crucian carp blood cells, which is comparable to the commercial kit. By using skin mucus swabbing and the disc method, we can obtain amplification-ready DNA from mucus to distinguish different species from our smallest fish (medaka, ~2.5 cm and crucian carp, ~7 cm) to our biggest fish (tilapia, ~15 cm). Furthermore, the viral pathogen
herpesvirus (CaHV) of crucian carp was detected using our method, which would make performing molecular diagnostic assays achievable in limited-resource settings including aquafarms and aqua stores outside the laboratory environment.
Bacterial load in meconium Jin, Wen‐Yu; Peng, Jing; Dai, Jinping ...
iMeta,
February 2024, Letnik:
3, Številka:
1
Journal Article
Recenzirano
Odprti dostop
The spike‐in plasmid method was utilized to perform an analysis on meconium and second‐pass feces, yielding both relative and absolute quantitative results. With the absolute quantitative data, the ...abundance of bacteria in 17 meconium samples and 17 second‐pass fecal samples were found to be 1.14 × 107 and 1.59 × 109 copies/g, respectively. The mode of delivery can significantly influence the alterations and compositions of gut bacteria in a newborn within 72 h.
Phenol and p-cresol are two common toxic small molecules related to various diseases. Existing reports confirmed that high L-tyrosine in the daily diet can increase the concentration of phenolic ...compounds in blood and urine. L-tyrosine is a common component of protein-rich foods. Some anaerobic bacteria in the gut can convert non-toxic l-tyrosine into these two toxic phenolic compounds, phenol and p-cresol. Existing methods have been constructed for measuring the concentration of phenolic compound in feces. However, there is still a lack of direct visual evidence to measure the phenolic compounds in the intestine. In this study, we aimed to construct a whole-cell biosensor for phenolic compounds detection based on the dmpR, the regulator from the phenol metabolism cluster. The commensal bacterium Citrobacter amalonaticus PS01 was selected and used as the chassis. Compared with the biosensor based on ECN1917, the biosensor PS01dmpR could better implant into the mouse gut through gavage and showed a higher sensitive to phenolic compound. And the concentration of phenolic compounds in the intestines could be observed with the help of in vivo imaging system using PS01dmpR. This paper demonstrated endogenous phenol synthesis in the gut and the strategy of using commensal bacteria to construct whole-cell biosensors for detecting small molecule compounds in the intestines.
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•A plasmid for detecting phenol and p-cresol was constructed.•The biosensor was constructed with a native bacterium chassis from the host.•The limit of quantitation of the biosensor was 948.4 nmol/L for phenol and 9.52 µmol/L for p-cresol.•The whole biosensor could be used in vivo to detect the level of toxic phenolic compounds in the host.
Implantable meshes used in tension‐free repair operations facilitate treatment of internal soft‐tissue defects. However, clinical meshes fail to achieve anti‐deformation, anti‐adhesion, and ...pro‐healing properties simultaneously, leading to undesirable surgery outcomes. Herein, inspired by the peritoneum, a novel biocompatible Janus porous poly(vinyl alcohol) hydrogel (JPVA hydrogel) is developed to achieve efficient repair of internal soft‐tissue defects by a facile yet efficient strategy based on top‐down solvent exchange. The densely porous and smooth bottom‐surface of JPVA hydrogel minimizes adhesion of fibroblasts and does not trigger any visceral adhesion, and its loose extracellular‐matrix‐like porous and rough top‐surface can significantly improve fibroblast adhesion and tissue growth, leading to superior abdominal wall defect treatment to commercially available PP and PCO meshes. With unique anti‐swelling property (maximum swelling ratio: 6.4%), JPVA hydrogel has long‐lasting anti‐deformation performance and maintains high mechanical strength after immersion in phosphate‐buffered saline (PBS) for 14 days, enabling tolerance to the maximum abdominal pressure in an internal wet environment. By integrating visceral anti‐adhesion and defect pro‐healing with anti‐deformation, the JPVA hydrogel patch shows great prospects for efficient internal soft‐tissue defect repair.
A peritoneum‐inspired Janus porous poly(vinyl alchohol) (PVA) hydrogel patch with unique asymmetric cell‐adhesion behaviors and excellent anti‐swelling characteristics demonstrates anti‐adhesion, pro‐healing, and anti‐deformation properties simultaneously, leading to superior repair effect of abdominal wall defects.
Visual electrophysiology measurements are important for ophthalmic diagnostic testing. Electrodes with combined optical transparency and softness are highly desirable, and sometimes indispensable for ...many ocular electrophysiology measurements. Here we report the fabrication of soft graphene contact lens electrodes (GRACEs) with broad-spectrum optical transparency, and their application in conformal, full-cornea recording of electroretinography (ERG) from cynomolgus monkeys. The GRACEs give higher signal amplitude than conventional ERG electrodes in recordings of various full-field ERG responses. High-quality topographic mapping of multifocal ERG under simultaneous fundus monitoring is realized. A conformal and tight interface between the GRACEs and cornea is revealed. Neither corneal irritation nor abnormal behavior of the animals is observed after ERG measurements with GRACEs. Furthermore, spatially resolved ERG recordings on rabbits with graphene multi-electrode array reveal a stronger signal at the central cornea than the periphery. These results demonstrate the unique capabilities of the graphene-based electrodes for in vivo visual electrophysiology studies.
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•Preparation of FePc/PDINH heterojunction photocatalyst via electrostatic interactions.•More efficient phenol degradation was achieved compared with FePc and PDINH.•FePc/PDINH ...promoted photogenerated carrier transfer and hindered recombination.•The influence of co-existing anions on photocatalytic performance was investigated.
The photocatalytic removal of organic pollutants from wastewater has received attention due to its environmental friendliness and low cost in recent years. Herein, a type-II heterojunction iron(II) phthalocyanine/perylene-3,4,9, 10-tetracarboxylic diimide (FePc/PDINH) composite photocatalyst was fabricated via electrostatic interactions, and its photocatalytic degradation performance and mechanism were explored. The FePc/PDINH photocatalyst exhibited a photocatalytic phenol removal efficiency of 95.7 % within 30 min. The degradation rate was 13.6 and 248.8-times higher than that of single PDINH and FePc under the optimal reaction conditions. The photocurrent response and photoluminescence spectroscopy results indicated that electron-hole pair recombination was hindered, and the migration rate of photogenerated carriers was improved due to the formation of a type-II heterojunction. The quenching and electron paramagnetic resonance spectroscopy results showed that ·O2− and 1O2 were the main reactive oxygen species involved in phenol degradation. Some common coexisting anions significantly inhibited phenol degradation. The density functional theory (DFT) calculations confirmed that electrostatic interactions between FePc and PDINH enhanced the photocatalytic activity. This allowed remarkable electron transfer at the interface and facilitated efficient interfacial charge migration and separation. This study offers a fresh design strategy for PDINH-based photocatalytic composites for organic pollutant degradation.
Adsorptive separation membranes are widely utilized for the removal of toxic dyeing pollutants from dyeing wastewater. However, developing novel adsorption membranes with large adsorption capacities ...and enhanced adsorption performance for dyes in actual wastewater poses a significant challenge. This study focuses on the fabrication of crown ether-containing copolymer porous membrane (CRPM) and investigation of the adsorption performance of dyes from aqueous solutions. The morphology structure and pore size distribution revealed that the membrane was endowed with rich micropores and hierarchical porous structures. Three typical cationic dyes (MB, RhB, CV) and an anionic dye (MO) were selected to evaluate the adsorption behavior. The results of adsorption isotherms and kinetics demonstrated that the adsorption data could be well-fitted using the Freundlich and pseudo-first-order kinetic models, the thermodynamic parameters revealed that the adsorption process of dyes on CRPM is a spontaneous endothermic reaction. The membrane exhibited excellent adsorption performance for cationic dyes, with RhB displaying a higher maximum adsorption capacity than previously reported porous membranes. Notably, dynamic adsorption-desorption filtration demonstrated a rapid removal efficiency, with RhB, MB, and CV achieving removal rates of 99.09%, 98.63%, and 99.14% respectively, after five cycles. The filtration volume of the CRPM membrane was 2.4-fold greater than that of a traditional PVDF membrane when applied to actual dyeing wastewater. DFT theoretical calculations were employed to elucidate the adsorption mechanism. These calculations confirmed the significant roles of electrostatic interactions, H-bonds and π–π interactions in facilitating the high-efficiency adsorption of cationic dyes. These findings highlight the potential of the crown ether-containing copolymer as a promising material for adsorption separation membranes in the treatment of dyeing wastewater.
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•The membrane possessed abundant micropores and a hierarchical porous structure.•The membrane indicated significant enhanced adsorption performance for cationic dyes.•Dynamic adsorption-desorption achieved >99% removal of cationic dyes in five cycles.•The membrane exhibited a 2.4-fold volume increase vs. traditional PVDF in dyeing wastewater filtration.•DFT calculations highlight electrostatic attraction and H-bonds' vital role in enhancing adsorption.
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