To analyse the ongoing epidemiology of KPC-producing Enterobacteriaceae after a non-ST258 KPC-3-producing Klebsiella pneumoniae outbreak in a university hospital in Madrid, Spain.
Enterobacterial ...isolates (one per patient based on bacterial identification and typing patterns) with carbapenem MICs higher than the EUCAST epidemiological cut-off values, a positive modified Hodge test and carbapenem/boronic acid combination disc test results were studied (16 March 2010 to 31 January 2012) and compared with KPC-producing isolates previously described in our institution (September 2009 to February 2010). The bacterial population structure (PFGE and multilocus sequence typing), carbapenemase genes and KPC plasmids were studied. Patients' clinical records were reviewed.
Twenty-four KPC-producing Enterobacteriaceae (20 K. pneumoniae, 2 Escherichia coli and 2 Enterobacter cloacae) from 23 patients (13 males, median age 72 years) were studied. Most KPC-producer strains were considered as colonizers. Six K. pneumoniae clones (ST11, ST20, ST384, ST454, ST659 and ST971), two E. coli clones (ST224 and ST357) and one E. cloacae clone were identified. blaKPC-3 was located on IncFIIK plasmids of ∼100 kb (E. coli ST357 and K. pneumoniae ST384, ST454, ST659 and ST971 clones) and on IncN plasmids of ∼40 kb (K. pneumoniae ST11 clone). Non-typeable plasmids of ∼20 kb containing blaKPC-2 were detected in scarcely represented clones (K. pneumoniae ST20, E. coli ST224 and E. cloacae).
During the 2 year period following the emergence of non-ST258 KPC-3-producing K. pneumoniae isolates in our institution, the blaKPC-3 and blaKPC-2 genes efficiently penetrated other Enterobacteriaceae lineages. Non-ST258 K. pneumoniae isolates were mostly responsible for the dissemination of KPC enzymes, producing a complex epidemiological picture.
The hake fishery (Merluccius hubbsi and Merluccius australis) is one of the most important ones for Spanish vessels operating in the Southwest Atlantic. Both species are widely distributed along the ...Patagonian shelf, although M. australis occupies mainly more southern waters. This paper presents a historical review of fishery data on the Spanish fishing fleet operating in the SW Atlantic collected and collated during the EC Study Project 99/016 “Data collection for stock assessment of two hakes (Merluccius hubbsi and M. australis) in International and Falkland waters of the SW Atlantic”, in order to analyse the evolution of catch and effort in the hake fishery since 1983 to 2000. Data on landings and effort by Spanish vessels from 1983 onwards were utilised to study trends and shifts within the fishery. These data were provided by Asociación Nacional de Armadores de Buques Congeladores de Pesca de Merluza (ANAMER), the most important Spanish ship-owners fishing association operating in distant fishing grounds. Catch and effort data collected by observer’s programmes carried out by Instituto Español de Oceanografía (IEO) and the Falkland Islands Government Fisheries Department (FIGFD), as well as by observers provided by the project were used to estimate CPUE by area and season.The data included landings in kgs by commercial size category of hake (both species together) and effort made by vessels appertaining to ANAMER in number of fishing days and number of vessels by vessel size category. Conversion factors obtained by scientific observers onboard of these vessels were used to obtain the whole catch from landings. Total effort of the Spanish fleet was estimated from ANAMER logbooks, assuming a similar pattern for the whole fleet and taking into account the different ratio of ANAMER fleet compared to the total Spanish fleet. An increase of the catches and effort was observed from 1983 to a maximum in 1990 coinciding with the closure of Namibian fisheries. After that, catches and effort decreased corresponding to the development of the Greenland halibut fishery in the NW Atlantic, until its stabilisation from 1993. CPUE showed different patterns of fishing activity by area and season.
The genus Patagonotothen is the most common Nototheniid on the Patagonian Shelf and slope and is part of the by-catch species in the bottom trawl fisheries. This paper presents preliminary results ...from the EC CRAFT project “Promoting higher added value to a finfish species rejected to sea”, aiming to develop the research and the technology necessary to promote higher added value to fishing activity by taking profit from a finfish species (Rockcod, Patagonotothen spp.) not known to consumers and currently discarded by the EU fishing fleet operating in the South West Atlantic in order to supply the EU seafood industry with a good quality raw material for human food manufacturing. Historical fishery data series (26 168 commercial hauls of which 12 745 were positive), including effort, catches and discards, as well as biological and environmental information, from 1988 onwards was used to describe and quantify patterns and spatio-temporal changes in the rockcod fishery. These data was collected by scientific observers on board commercial vessels.GIS analysis was carried out in order to study the seasonal geographical changes in the distribution of abundance, SST and densities calculated from CPUE. Fishery and environmental data as well as SST data derived from the NOAA Advanced Very High Resolution Radiometer (AVHRR), were analysed in order to find monthly variations in spatial and depth distribution of Patagonotothen spp. Preliminary results demonstrated that, within areas of occurrence, there are significant correlations between fish abundance (CPUE), the oceanographic conditions of the area (SST gradients) and depth in certain months.
The genus Patagonotothen is the most common Nototheniid on the Patagonian Shelf and slope and is part of the by-catch species in the bottom trawl fisheries. This paper presents preliminary results ...from the EC CRAFT project “Promoting higher added value to a finfish species rejected to sea”, aiming to develop the research and the technology necessary to promote higher added value to fishing activity by taking profit from a finfish species (Rockcod, Patagonotothen spp.) not known to consumers and currently discarded by the EU fishing fleet operating in the South West Atlantic in order to supply the EU seafood industry with a good quality raw material for human food manufacturing. Historical fishery data series (26 168 commercial hauls of which 12 745 were positive), including effort, catches and discards, as well as biological and environmental information, from 1988 onwards was used to describe and quantify patterns and spatio-temporal changes in the rockcod fishery. These data was collected by scientific observers on board commercial vessels.GIS analysis was carried out in order to study the seasonal geographical changes in the distribution of abundance, SST and densities calculated from CPUE. Fishery and environmental data as well as SST data derived from the NOAA Advanced Very High Resolution Radiometer (AVHRR), were analysed in order to find monthly variations in spatial and depth distribution of Patagonotothen spp. Preliminary results demonstrated that, within areas of occurrence, there are significant correlations between fish abundance (CPUE), the oceanographic conditions of the area (SST gradients) and depth in certain months
The fishing grounds of the Patagonian Shelf support some of the most important fisheries in the world. The great abundance of marine resources among parallels 35º and 54º South, is associated with ...the Subtropical Convergence formed by the Brazil and Falkland/Malvinas currents. The mixing of the flow of La Plata River and the western branch of the Falkland/Malvinas Current generates areas of high plankton production on the shelf. Hakes (Merluccius hubbsi and Merluccius australis) and cephalopods (Illex argentinus and Loligo gahi) have been found the main commercial species, with important amounts of accompanying species in the catches such as Patagonian toothfish (Dissostichus eleginoides), Kingclip (Genypterus blacodes), Hoki (Macruronus magellanicus), Red cod (Salilota australis), Southern blue whiting (Micromesistius australis), etc. These fisheries are currently among the most important to the Spanish bottom trawler freezing fleet that has around 40 vessels fishing in this area, besides another 20 that operate in joint ventures sailing Falkland flag. It is estimated that this fleet generates approximately 2,000 direct offshore jobs, and more than 10,000 indirect onshore jobs. This paper presents results from the EC Study Project 99/016 “Data collection for stock assessment of two hakes (Merluccius hubbsi and M. australis) in international and Falkland waters of the SW Atlantic”. Historical fishery and biological data series available from the Instituto Español de Oceanografía (IEO) and the Falkland Islands Fishery Department (FIFD) were used during the project to describe fishing patterns and spatio-temporal changes in the abundance and distribution of target and accompanying species, as well as to give an overview of these important fisheries.
Study Project 99/016 “Data collection for stock assessment of two hakes (Merluccius hubbsi and M. australis) in international and Falkland waters of the SW Atlantic” ran from January 2000 to December ...2001. The main objective of the project was the collection and collation of already existing and newly acquired fishery and biological data needed for preliminary assessment of two hake species occurring in the study area. In addition to this basic remit, additional objectives included the creation of a common database, study of spawning seasons and areas, discard pattern and length-frequency composition of target and non-target species, estimation of annual by-catch rates, analysis of trophic relationships, marine mammals by-catch and sightings, morphometric analysis for stock differentiation, and developing GIS applications for analysis of the data collected. Historical fishery and biological data series available from IEO and FIGFD (since 1988 and 1987 respectively) were provided to the project. New fishery and biological data were collected by scientific observers provided by IEO, ANAMER and FIGFD, and placed on board Spanish fishing vessels operating in the study area during the project period. Data on fishing activity included effort, catches and discards of target and non-target species on a haul-by-haul basis. Biological information (size, sex, maturity stage, etc) of target and non-target species was recorded on a daily basis. Data on landings and effort were provided by ANAMER to its subcontractor (MG OTERO) for processing and estimation of total catch and effort of the whole Spanish fishing fleet in the area; MG OTERO was also responsible for organisation of observers in collaboration with ANAMER staff in Vigo and Port Stanley. Ancillary data on location, time of fishing, depth, SST, SBT, sea roughness, wind, etc, was recorded on a haul-by-haul basis. This type of information was essential for development of GIS at AU to relate the species distribution to physical and environmental factors. Other information collected was about by-catches and sightings of small cetaceans and seabirds, and biological samples such as otoliths, stomachs and whole specimens of hakes for subsequent studies on growth (IEO, FIGFD), diet and morphometrics (AU). All the historical and new data collected during the project were collated and integrated into a common database designed by all participants and built at IEO. The information was used for preliminary assessment of two hake populations co-ordinated by RRAG during a workshop held in London in July 2001. All these data will be analysed and written up for future publications. Discard rates of target species were generally low in all areas and seasons with the highest discard rate for Notothen sp. (around 100% of the catch). Illex squid was found to be the major by-catch for hake fishery in the 46 S area. IEO observers reported data on incidental catches of marine mammals and sea birds since 1993 and the analysis of this information was made by AU. The observed mortality in the fishing gears comprised small numbers of black-browed albatross, gentoo penguin and the hourglass dolphin. The species most frequently sighted was the Peale’s dolphin, although this species did not appear in by-catches, followed by the hourglass dolphin. The project provided an opportunity to collect and integrate for the first time at European level the necessary fishery and biological data for the development of partial stock assessment for the future rational management of the fisheries in the area. Such management is needed for the sustainability of the commercial fisheries, the conservation of the onshore and offshore jobs and the supply of fish to the most important markets worldwide.
Abstract We report the use of tigecycline, firstly with colistin and finally alone, in a patient with a persistent breakthrough bacteremia due to a Klebsiella pneumoniae isolate harboring a ...metallo-β-lactamase (VIM-1) and an extended-spectrum β-lactamase (SHV-12). Time-kill studies demonstrated that the combination of both compounds was synergistic along the first 12 h, suppressing the regrowth observed after 3 to 6 h when colistin was tested alone.
This project aimed to the development of the research and the technology necessary to promote higher added value to fishing activity. This is to be achieved by obtaining profit from a finfish species ...(“Rockcod”, Patagonotothen spp.) not known to consumers and currently discarded by the EU fishing fleet operating in the South West Atlantic, in order to supply the EU seafood industry with a good quality raw material for human food manufacturing. Use of this species, caught as a by-catch in the existing fisheries targeting hakes and cephalopods, should also increase the profitability of the fleet, contribute to maintaining employment and help to counterbalance the negative effects of fishing activity and discards in the ecosystem. The main scientific-technological objectives and expected achievements were the following: - Description of the fisheries - Improved knowledge of the biology of the species - Biomass assessment - Estimation of catches and discards - Analysis of the spatial and temporal distribution of the resource. Fishery forecasting and testing - Sensorial, Microbiological, Nutritional and Biochemical Evaluation of Rock cod - Development of the technical modifications on board commercial vessels - Development of new processed products from frozen Rock cod
Introducción: La peritonitis es una de las complicaciones más frecuentes y graves asociadas a la diálisis peritoneal (DP), siendo la primera causa de pérdida de catéter y de finalización de la ...técnica. La incidencia y etiología de cada episodio varía en función de cada región y evoluciona a lo largo del tiempo. Por esta razón, es de vital importancia conocer los factores de riesgo y pronósticos de peritonitis en cada momento. Métodos: Se realizó un estudio observacional de cohorte, multicéntrico, prospectivo, con un seguimiento máximo de 7 años (2003-2010) que incluyó 1177 pacientes, con 476 episodios de primera peritonitis (total: 1091 peritonitis). Resultados: Se presenta la descripción de las características del primer episodio de peritonitis en una serie amplia y actual. Los factores que se asociaron a un menor tiempo hasta la primera peritonitis en análisis multivariante fueron la comorbilidad cardiovascular previa (hazard ratio HR 1,25 1,04-1,58), la procedencia de hemodiálisis (HR 1,39 1,10-1,76) o trasplante renal previo (HR 1,38 1,10-1,93), iniciar DP con técnica manual (HR 1,39 1,13-1,73) y la edad de inicio > 70 años (HR 1,53 1,23-1,90). El primer episodio de peritonitis presenta una tasa de recidiva del 7,8 %, retirada de catéter del 11,7 % y una mortalidad en el primer mes tras el episodio del 1,3 %. La evolución de la peritonitis depende fundamentalmente del tipo de germen. Se estima un riesgo mayor para gramnegativos (odds ratio OR 5,31 2,26-12,48) y el agregado de peritonitis polimicrobianas, por hongos o por micobacterias (OR 38,24 13,84-105,63), comparados con la referencia de grampositivos. Conclusión: El desarrollo del primer episodio de peritonitis depende de las características del paciente al inicio de diálisis, la comorbilidad y la técnica utilizada. Los pacientes que presentan mayor riesgo deben recibir una atención especial en los procesos de entrenamiento y seguimiento.
We describe two patients who developed multiple itching nodules following immunization with vaccines adsorbed on aluminium hydroxide. Both patients had been treated with vaccines for extrinsic asthma ...and rhinitis for 4 and 10 years respectively. The lesions were persistent and lasted for several years. Histopathological findings were those of a foreign body reaction. Aluminium was most probably involved in the pathogenesis of these lesions because its presence could be demonstrated in macrophages using energy-dispersive X-ray microanalysis. Although some symptomatic relief was achieved with topical corticosteroids and oral antihistamines, treatment was unsuccessful.
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