ABSTRACT Plant growth-promoting bacteria (PGPB) are found in plant tissues and promote plant growth by secretion of hormones and enzymes, or by facilitating the nutrient uptake. This study assessed ...forty PGPB isolates to determine their effects on maize and sorghum growth. These isolates were also compared with uninoculated plants, as negative (-N; without N fertilization) and positive (+N; with N fertilization) controls. Plant height, stem diameter, shoot and root dry mass, leaf N accumulation and chlorophyll content were evaluated. For both the maize and sorghum, the height, stem diameter and shoot dry mass in plants inoculated with PGPB were similar to those of uninoculated plants supplied with N, and the responses for root mass were higher than in plants supplied with N. However, the PGPB isolates did not promote N accumulation and chlorophyll content similar to those of uninoculated plants supplied with N. The IPACC26 and IPACC30 isolates, both identified as Bacillus subtilis, resulted in better responses for plant growth and N accumulation than the other isolates.
RESUMO Bactérias promotoras de crescimento em plantas (BPCP) são encontradas em tecidos vegetais e promovem o crescimento vegetal por meio da secreção de hormônios e enzimas, ou auxiliando a absorção de nutrientes. Avaliaram-se quarenta isolados de BPCP para determinar seus efeitos sobre o crescimento de milho e sorgo. Estes isolados foram também comparados com plantas não inoculadas, como controles negativos (-N; sem fertilização nitrogenada) e positivos (+N; com fertilização nitrogenada). A altura, diâmetro de caule, massas secas da parte aérea e raízes, acúmulo de N e conteúdo de clorofila foram avaliados. Em ambos o milho e o sorgo, a altura, diâmetro do caule e a massa seca da parte aérea em plantas inoculadas com BPCP foram similares aos das plantas não inoculadas e fertilizadas com N, e as respostas da massa das raízes foram maiores que das plantas fertilizadas com N. Entretanto, os isolados de BPCP não promoveram acúmulo de N e conteúdo de clorofila semelhantes aos das plantas não inoculadas e fertilizadas com N. IPACC26 e IPACC30, ambos identificados como Bacillus subtilis, apresentaram melhores respostas para o crescimento das plantas e acúmulo de N do que os outros isolados.
Thyroid dysfunction is common in patients with heart failure (HF). Impaired conversion of free T4 (FT4) into free T3 (FT3) is thought to occur in these patients, decreasing the availability of FT3 ...and contributing to HF progression. In HF with preserved ejection fraction (HFpEF), it is not known whether changes in conversion of thyroid hormones (THs) are associated with clinical status and outcomes.
The objective of this study was to evaluate the association of FT3/FT4 ratio and TH with clinical, analytical, and echocardiographic parameters, as well as their prognostic impact in individuals with stable HFpEF.
We evaluated 74 HFpEF participants of the NETDiamond cohort without known thyroid disease. We performed regression modeling to study the associations of TH and FT3/FT4 ratio with clinical, anthropometric, analytical, and echocardiographic parameters, and survival analysis to evaluate associations with the composite of diuretic intensification, urgent HF visit, HF hospitalization, or cardiovascular death over a median follow-up of 2.8 years.
The mean age was 73.7 years and 62% were men. The mean FT3/FT4 ratio was 2.63 (standard deviation: 0.43). Subjects with lower FT3/FT4 ratio were more likely to be obese and have atrial fibrillation. Lower FT3/FT4 ratio was associated with higher body fat (β = -5.60 kg per FT3/FT4 unit, p = 0.034), higher pulmonary arterial systolic pressure (PASP) (β = -10.26 mm Hg per FT3/FT4 unit, p = 0.002), and lower left ventricular ejection fraction (LVEF) (β = 3.60% per FT3/FT4 unit, p = 0.008). Lower FT3/FT4 ratio was associated with higher risk for the composite HF outcome (HR = 2.50, 95% CI: 1.04-5.88, per 1-unit decrease in FT3/FT4, p = 0.041).
In patients with HFpEF, lower FT3/FT4 ratio was associated with higher body fat, higher PASP, and lower LVEF. Lower FT3/FT4 predicted a higher risk of diuretic intensification, urgent HF visits, HF hospitalization, or cardiovascular death. These findings suggest that decreased FT4 to FT3 conversion might be a mechanism associated with HFpEF progression.
Heart failure (HF) patients often experience poor health-related quality-of-life (HR-QoL). The Kansas City Cardiomyopathy Questionnaire (KCCQ) is frequently used for assessing HR-QoL in HF. Whether ...KCCQ scores vary in a clinical meaningful manner according to the setting (home vs office) where patients respond to the questionnaire is currently unknown.
Assess the differences in the responses to KCCQ-23 questionnaire when completed at home or office.
Randomized parallel-group study, including patients with HF with reduced ejection fraction (HFrEF). Primary outcome was home vs office comparison of overall summary score (KCCQ-OSS). Main secondary outcomes were clinical summary score (KCCQ-CSS) and total symptom score (KCCQ-TSS).
A total of 100 patients were included in the study: 50 home vs 50 office. Mean age was 71 yrs. Most baseline characteristics were well balanced between groups, except male sex, MRA use, and prior HF hospitalizations which were more frequent in the home group. No statistically-significant between-group differences were found regarding KCCQ-OSS (median percentile25–75) scores: home 69.1 (42.0–86.5) vs office 63.1 (44.3–82.3) points, P-value = 0.59, or main secondary outcomes: KCCQ-CSS home 62.2 (46.5–79.9) vs office 68.1 (51.9–79.2) points, P-value = 0.69, and KCCQ-TSS home 84.7 (59.7–97.2) vs office 76.4 (66.7–94.4) points, P-value = 0.85. Results remained similar after adjustment for differences in baseline characteristics and using non-parametric regressions.
No major differences were found in KCCQ-23 scores regardless of whether the questionnaire was completed at home or office. These findings can be useful to make HR-QoL more accessible, allowing patients to respond at home using email or cell-phone applications.
•KCCQ is increasingly used to assess patient reported outcomes.•Whether KCCQ scores vary according to the setting where the questionnaire is filled-in is unknown.•No differences between KCCQ scores were found irrespective of the questiionnaire being filled-in at home or at the office.
Plant growth-promoting bacteria (PGPB) are found in plant tissues and promote plant growth by secretion of hormones and enzymes, or by facilitating the nutrient uptake. This study assessed forty PGPB ...isolates to determine their effects on maize and sorghum growth. These isolates were also compared with uninoculated plants, as negative (-N; without N fertilization) and positive (+N; with N fertilization) controls. Plant height, stem diameter, shoot and root dry mass, leaf N accumulation and chlorophyll content were evaluated. For both the maize and sorghum, the height, stem diameter and shoot dry mass in plants inoculated with PGPB were similar to those of uninoculated plants supplied with N, and the responses for root mass were higher than in plants supplied with N. However, the PGPB isolates did not promote N accumulation and chlorophyll content similar to those of uninoculated plants supplied with N. The IPACC26 and IPACC30 isolates, both identified as Bacillus subtilis, resulted in better responses for plant growth and N accumulation than the other isolates.
Este trabalho investigou a capacidade de ferramentas baseadas em modelos de linguagem e técnicas tradicionais de recuperação de informações, como o Generative Pre-trained Transformer (GPT-3) e ...CROKAGE respectivamente, em gerar respostas legíveis para perguntas de programação (Q&A). Para isso, foi realizada uma análise qualitativa e quantitativa de correção sintática e semântica estática, correção semântica, foco de código e legibilidade em um conjunto de questões e respostas, bem como um experimento piloto com sujeitos humanos. Os resultados sugerem que essas ferramentas são capazes, de forma complementar, de gerar código completo e executável que pode ser útil para desenvolvedores iniciantes, mas ainda enfrentam desafios em relação à semântica e ao foco no código.
Nodulation results of a host-specific association between rhizobia and leguminous plants. However, it has been observed a rich and diverse bacterial community inside nodules. This study assessed the ...bacterial community in nodule of cowpea grown in sites with distinct soil properties to address the hypothesis that these differences could influence the bacterial community inside the nodules. Soil samples and nodules of cowpea were collected in six different sites from Northeastern, Brazil. There was a different bacterial community structure and composition comparing bulk soil and nodules, with lower diversity in the last. However, the bacterial communities inside the nodules were not different comparing all sites. The bacterial communities found inside the nodules belonged to Proteobacteria (Bradyrhizobium and Rhizobium with 97% and 2.63%, respectively), Actinobacteria (Conexibacter and Amycolatopsis with 0.047% and 0.015%, respectively), and Firmicutes (Bacillus and Salipaludibacillus with 0.042% and 0.040%, respectively). Our analysis also showed that bulk soil and nodules presented 99.3% and 0.03% of exclusive OTUs, respectively, while these pools shared 0.4% of the bacterial groups. These results indicate that cowpea selected a specific bacterial group that colonizes the nodules, and it is not influenced by soil properties.
Propolis is a plant-derived bee product rich in volatile constituents that are vital to its aroma and biological properties. The aims of this study were to determine, for the first time, the volatile ...oil composition of green propolis (produced by
Apis mellifera
) from the Caatinga biome in northeastern Brazil and to evaluate the influence of the rainy and dry seasons on its composition. Forty-nine components belonging to monoterpene (hydrocarbons and oxygenates), sesquiterpene (hydrocarbons and oxygenates), and aliphatic hydrocarbon classes were identified. Monoterpenes tended to be more abundant in the dry season, whereas the opposite was true for aliphatic hydrocarbons. Sesquiterpenes were identified as the predominant volatile component in green propolis (88.48–92.29%) and no significant seasonal variation was observed. This lack of variation probably does not have a significant influence on the properties of the matrix. The major components identified were β-caryophyllene (12.28–15.31%), 7-
epi
-α-selinene (11.82–16.31%), and δ-cadinene (7.12–28.09%).
Graphical abstract
Microgravity has proved to be an ideal condition to grow crystals. In article number 2101777, Raphael Pfattner, Tiago Sotto Mayor, Daniel Ruiz‐Molina, Josep Puigmartí‐Luis, and co‐workers demonstrate ...how to generate simulated microgravity on Earth to grow 2D porous crystalline molecular frameworks such as 2D metal–organic frameworks and 2D covalent organic frameworks.
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