A high molecular weight mucin fraction was prepared from human whole saliva using relatively mild conditions. The method involves ultracentrifugation of human whole saliva in the presence of 7.2 M ...urea and 0.5 M sodium chloride. The resulting preparation consists of a highly purified salivary mucin fraction, as judged by several purity criteria: molecular weight analysis of the final preparation by gel electrophoresis and analytical gel filtration indicated an apparent molecular weight greater than 10(6). Analytical isopycnic density centrifugation demonstrated that the preparation consisted of a mucin fraction with a buoyant density of approximately 1.47 g/ml. The final preparation comprised 12.8% protein, 31.8% N-acetylglucosamine, 11.5% N-acetylgalactosamine, 9.5% fucose, 21.4% galactose, 0.8% mannose, 10.3% N-acetylneuraminic acid, 1.7% sulphate and 0.16% fatty acids.
Book Reviews C van Dijk; Reid, A; J van Goor ...
Bijdragen tot de Taal-, Land- en Volkenkunde,
04/1981, Letnik:
137, Številka:
2/3
Book Review
Recenzirano
Odprti dostop
...the documents edited here have been divided into two sections, namely "politics" and "internal court affairs", and these sections in turn into subsections according to subject. ...the quality of ...this first volume is such that I look forward with interest to the appearance of the second volume announced in the preface, which "will comprise all the materials relating to economic, military and agrarian affairs". ...this rapid survey of the economic and political developments described clearly and in detail: the first English incursion in Perak takes place in 1781. ...a few words on what it can offer to structural and comparative studies of western Indonesian states.
