Selenium nanoparticles (SeNPs) represent novel selenium (Se) formulation characterized by improved biocompatibility and a wider therapeutic range in comparison to inorganic Se. The aim of this work ...was to investigate the possibilities of functionalization of SeNPs with olive pomace extract (OPE), rich in health-promoting polyphenols, and to obtain innovative forms of nutraceuticals. Cytotoxic and antioxidative activities of four types of SeNPs (polyvinylpyrrolidone stabilized (PVP SeNPs), polysorbate stabilized (PS SeNPs), polyvinylpyrrolidone stabilized and functionalized using OPE (f PVP SeNPs) and polysorbate stabilized and functionalized using OPE (f PS SeNPs) were investigated. SeNPs showed lower toxicity on human hepatocellular carcinoma (HepG2) and human colorectal adenocarcinoma (Caco2) cells compared to selenite. Functionalization with polyphenols significantly improved their direct antiradical (f PVP SeNPs: 24.4 ± 1.84 and f PS SeNPs: 30.9 ± 2.47 mg TE/mmol Se) and reducing properties (f PVP SeNPs: 50 ± 3.16 and f PS SeNPs: 53.6 ± 3.22 mg GAE/mmol) compared to non-functionalized SeNPs. The significant impact of tested SeNPs on intracellular antioxidative mechanisms has been observed and it was dependent on both cell type and physico-chemical properties of SeNPs, indicating the complexity of involved mechanisms.
Garlic is a valuable source material for medicines due to its known antitumor, hypolipidaemic, antioxidant, and immunomodulatory effects. This study compares the protective effects of conventionally ...grown (CG) and
propagated garlic (PG) against hydrogen peroxide-induced cytotoxicity in HepG2 cells and their antioxidant activity. Garlic used in this study was obtained by planting garlic cloves or by planting the transplants of PG directly in the field. At the end of the vegetation period, CG and PG were sampled and extracts prepared for the experiment. Compared to conventionally grown garlic bulbs, PG leafy part yielded significantly higher content of polyphenols, flavonoids and alliin, and also showed equal or higher antioxidant activity, measured by the cell viability test, GSH and ROS level. Moreover, PG can be produced in less time (shorter vegetation period) and with significantly less material (cloves). Significantly higher content of alliin, polyphenols, and flavonoids and significantly higher yield of plant biomass in PG has a great potential to become a new production model with improved garlic properties as a medicine material.
The increasing development of different nanomaterials, such as silver nanoparticles (AgNPs), and their practical use in agriculture and biotechnology has created a strong need for elucidations of ...biological effects and risk assessments of AgNPs in plants. This study was aimed to investigate AgNPs effects on metal uptake and their biodistribution in pepper plants as well as on morphological parameters and hormonal responses of the isoprenoid cytokinin (CK) family. In addition, the comparison of effects silver form, nanoparticles vs. ionic, has also been examined. To the best of our knowledge, this is the first study describing CK responses in plants exposed to metallic NPs.
The obtained results indicate that both AgNPs and Ag+ ions significantly increased total content of Ag+ in pepper tissues in a dose-dependent manner and affected on plant development by decreasing both plant height and biomass in a similar way. This study evidenced for the first time the role of CKs in abiotic stress in plants caused by AgNPs. The hormonal analysis, conducted by an ultra-high performance liquid chromatography-electrospray tandem mass spectrometry, revealed a significant increase in total CKs in the leaves and also highlighted the importance of cis-zeatin type CKs in plants treated with AgNPs. Our observations suggest potential risks of AgNPs on plant ecosystems upon their release into the environment.
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•Citrate-coated silver nanoparticles significantly affected development of pepper.•Nano and ionic form of Ag showed similar phytotoxicity effects in pepper plants.•Both Ag forms increased total Ag in pepper tissues in a dose-dependent manner.•This is first study establishing the role of cytokinins in abiotic stress in plants.•Results indicate possible importance of cZ cytokinins in nanostressed plants.
A stationary field experiment of a reduced soil tillage was implemented at a Hypogley (Hypogleyic soils A–Gso–Gr soil horizon sequence) soil type of Eastern Croatia during three seasons and set up as ...a split-plot randomized block design in four repetitions. The tillage systems (TS) were as follows: 1) conventional tillage, i.e., plowing at 30 cm (CT), 2) disking up 10-12 cm (DT), 3) soil loosening up to 35 cm (LT), 4) no-tillage (NT). The experiment was designed to compare the penetration resistance (PR), soil moisture (SM), and bulk density (BD) at different TSs and soil depths. A cone penetrometer was used to measure the PR with 10 prods per TS, accompanied with a measurement of SM with a soil auger on every 10 cm, with four samples up to a 40-cm depth. The BD was determined by metal cylinders on every 10 cm up to a 30-cm depth, being weighed and dried thereafter to obtain an absolutely dry sample, and then calculated using absolutely a dry soil sample mass (m_s) and the soil volume (V). The PR and SM were significantly influenced by the TS and soil depth. The CT had the significantly lowest PR at all depths, while the DT has manifested a significantly higher PR at a soil depth amounting to 10 to 20 cm. The PR on NT were significantly diverse from the CT at all soil depths. The BD varied significantly concerning the TS and the soil depth. Subsequent to the three years, the CT had a significantly smaller BD at a depth amounting from 0 to 10 cm, and a significantly higher BD at 20- to 30-cm depth, compared to reduce the TS.
Stacionarni poljski pokus reducirane obrade tla proveden je u istočnoj Hrvatskoj na hipogleju tijekom triju sezona. Pokus je postavljen kao randomizirani blok-sustav u četiri ponavljanja. Sustavi obrade tla (TS) bili su sljedeći: 1) konvencionalna obrada tla oranjem na 30 cm (CT); 2) tanjuranje na 10–12 cm (DT); 3) rahljenje tla na 35 cm (LT); 4) no-tillage (NT). U eksperimentu su praćeni otpori tla (PR), vlaga tla (SM) i volumna gustoća tla (BD) na različitim TS-ovima i dubinama tla. Konusni penetrometar korišten je za mjerenje PR-a s 10 uboda po TS-u, praćeno sondiranjem za SM na svakih 10 cm, s četirima uzorcima do 40 cm dubine. BD je određen metalnim cilindrima na svakih 10 cm do 30 cm dubine. Izvagani uzorak je osušen do apsolutno suhoga uzorka, a zatim izračunan BD koristeći se apsolutno suhom masom uzorka tla (m_s) i volumena tla (V). PR i SM su bili pod značajnim utjecajem TS-a i dubine tla. CT je imao najniži PR na svim dubinama, DT je pokazao značajno veći PR na dubini tla od 10 do 20 cm u odnosu na CT. PR na NT bio je značajno različit od CT-a na svim dubinama tla. BD se znatno razlikovao po TS-u i dubini tla. Nakon tri godine CT je imao signifikantno manji BD na dubini 0-10 cm te signifikantno veći BD na dubini 20-30 cm u odnosu prema reduciranim sustavima obrade tla.
Olive pomace extract (OPE) was investigated as a potential surface modifier for the development of the green synthesis process of selenium nanoparticles (SeNPs). In order to evaluate them as ...potential nutraceuticals, the obtained nanosystems were characterized in terms of size distribution, shape, zeta potential, stability in different media, gastrointestinal bioaccessibility and biocompatibility. Systems with a unimodal size distribution of spherical particles were obtained, with average diameters ranging from 53.3 nm to 181.7 nm, depending on the type of coating agent used and the presence of OPE in the reaction mixture. The nanosystems were significantly affected by the gastrointestinal conditions. Bioaccessibility ranged from 33.57% to 56.93% and it was significantly increased by functionalization of with OPE. Biocompatibility was investigated in the HepG2 and Caco2 cell models, proving that they had significantly lower toxicity in comparison to sodium selenite. Significant differences were observed in cellular responses depending on the type of cells used, indicating differences in the mechanisms of toxicity induced by SeNPs. The obtained results provide new insight into the possibilities for the utilization of valuable food-waste extracts in the sustainable development of nanonutraceuticals.
Selenium (Se) is an important micronutrient which is essential for most living organisms and occurs in both organic and inorganic forms in the water system, soils, biomass, and the atmosphere. In ...addition to being essential for humans and animals, Se is beneficial for plants and is mostly involved in antioxidant activity/response, as well as a growth promoter. Se deficiency in the diet is a global problem, and Se levels in soils generally reflect its presence in food and, thus, availability to humans. Se participates in the antioxidant response mechanisms of the organism, heavy-metal detoxification, and regulation of the reproductive and immune system, as well as ensures the proper function of the thyroid gland. Plants are the main dietary source of Se for humans. Biofortification is a key strategy to increase Se in edible parts of plants. Agronomic biofortification provides an effective route to increase Se content in edible crop products via application of Se-enriched fertilizers to soil or by foliar application. The most common cereals in the human diet are wheat, rice, maize, and barley, making them the most suitable targets for agronomic biofortification. This review focuses on summarizing the most efficient form and method of Se application via agronomic biofortification corroborated by a meta-analysis of the literature reports. In the assessed literature, foliar application showed better results compared to application in soil. The selenate form appears to be the more efficient form of Se for biofortification than selenite in the most common cereals in human diet: wheat, rice, maize, and barley.
This study, motivated to fill the knowledge gap on environmental safety of selenium nanoparticles (SeNPs), provides information on the stability and environmental safety of four differently coated ...SeNPs rendering both positive and negative surface charges. The stability and dissolution behaviour of SeNPs were determined in an aquatic model media of different ionic strength to provide information regarding the environmental fate of SeNPs in different environmental conditions. The environmental safety of SeNPs was evaluated by acute regulatory toxicity tests using Daphina magna and Vibrio fischeri as model organisms. Agglomeration was observed for all studied SeNPs in test media with higher ionic strength caused by the disruption of surface charge leading to electrostatic instability. Toxicity of SeNPs on both aquatic species was dose-dependent and increased with exposure time. The obtained data indicated that all of the tested SeNPs could be classified as harmful to the natural bacteria V. fischeri and harmful to toxic to crustaceans D. magna, but dependent on the coating agent used for SeNPs stabilization. Although SeNPs have attracted great interest for use in biomedicine, this study demonstrated that their ecotoxicological effects should be considered during the design of new of SeNPs-based products.
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•We provide data on stability and environmental safety of four differently coated SeNPs.•Exposure settings included different aquatic model media.•Agglomeration of all SeNPs occurred in media with higher ionic strength.•All tested SeNPs were harmful to Vibrio fischeri and harmful/toxic to Daphnia magna.
Starch is a polysaccharide that is widely used in food and other industries; therefore, due to its great potential, it is attempted to be maximally isolated from various foods rich in starch. ...Commonly, potatoes are used for starch production due to the relatively high starch content in tubers, and the process itself is complex and includes several steps. The aim of this study was to isolate and characterize starch from eight potato varieties. First, the basic chemical composition of the potato samples was determined, and then the isolation was carried out under laboratory conditions. The isolated starch was air dried, then ground and sieved through a 400 µm sieve. The basic chemical composition, amylose content, starch color, swelling capacity and solubility index, clarity of starch pastes, texture of starch gels and thermo-physical properties (gelatinization and retrogradation) were determined in the obtained starch samples. The results showed that the SL 13–25 potato variety had the lowest starch content, while the Stilleto variety had the highest starch content. The content of protein, fat, ash and crude fiber was relatively low in all of the isolated starches, indicating their high purity. Also, the difference in the color of the isolated starches was difficult to see. The highest amylose content had starch from Saprodi, while the lowest was observed in starch from SL 13–25. Starch from the cv. Dartiest had the highest, while starch from the cv. Sereno had the lowest gel strength. The starch of the cv. Dartiest also had the highest clarity value. The retrogradation transition temperatures and enthalpies were lower compared to the gelatinization temperatures and enthalpies. With the increasing temperature, both the swelling capacity and the solubility index of all the samples increased. According to the obtained differences, this study confirmed the significant influence and role of different cultivars on starch characteristics.
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