The bacterial type III secretion system, or injectisome, is a syringe shaped nanomachine essential for the virulence of many disease causing Gram-negative bacteria. At the core of the injectisome ...structure is the needle complex, a continuous channel formed by the highly oligomerized inner and outer membrane hollow rings and a polymerized helical needle filament which spans through and projects into the infected host cell. Here we present the near-atomic resolution structure of a needle complex from the prototypical Salmonella Typhimurium SPI-1 type III secretion system, with local masking protocols allowing for model building and refinement of the major membrane spanning components of the needle complex base in addition to an isolated needle filament. This work provides significant insight into injectisome structure and assembly and importantly captures the molecular basis for substrate induced gating in the giant outer membrane secretin portal family.
We report the discovery of TOI 837b and its validation as a transiting planet. We characterize the system using data from the NASA Transiting Exoplanet Survey Satellite mission, the ESA Gaia mission, ...ground-based photometry from El Sauce and ASTEP400, and spectroscopy from CHIRON, FEROS, and Veloce. We find that TOI 837 is a T = 9.9 mag G0/F9 dwarf in the southern open cluster IC 2602. The star and planet are therefore million years old. Combining the transit photometry with a prior on the stellar parameters derived from the cluster color-magnitude diagram, we find that the planet has an orbital period of and is slightly smaller than Jupiter ( ). From radial velocity monitoring, we limit to less than 1.20 MJup (3 ). The transits either graze or nearly graze the stellar limb. Grazing transits are a cause for concern, as they are often indicative of astrophysical false-positive scenarios. Our follow-up data show that such scenarios are unlikely. Our combined multicolor photometry, high-resolution imaging, and radial velocities rule out hierarchical eclipsing binary scenarios. Background eclipsing binary scenarios, though limited by speckle imaging, remain a 0.2% possibility. TOI 837b is therefore a validated adolescent exoplanet. The planetary nature of the system can be confirmed or refuted through observations of the stellar obliquity and the planetary mass. Such observations may also improve our understanding of how the physical and orbital properties of exoplanets change in time.
ABSTRACT
We report on the detection of pulsations of three pulsating subdwarf B stars observed by the Transiting Exoplanet Survey Satellite (TESS) satellite and our results of mode identification in ...these stars based on an asymptotic period relation. SB 459 (TIC 067584818), SB 815 (TIC 169285097), and PG 0342 + 026 (TIC 457168745) have been monitored during single sectors resulting in 27 d coverage. These data sets allowed for detecting, in each star, a few tens of frequencies that we interpreted as stellar oscillations. We found no multiplets, though we partially constrained mode geometry by means of period spacing, which recently became a key tool in analyses of pulsating subdwarf B stars. Standard routine that we have used allowed us to select candidates for trapped modes that surely bear signatures of non-uniform chemical profile inside the stars. We have also done statistical analysis using collected spectroscopic and asteroseismic data of previously known subdwarf B stars along with our three stars. Making use of high precision trigonometric parallaxes from the Gaia mission and spectral energy distributions we converted atmospheric parameters to stellar ones. Radii, masses, and luminosities are close to their canonical values for extreme horizontal branch stars. In particular, the stellar masses are close to the canonical one of 0.47 M⊙ for all three stars but uncertainties on the mass are large. The results of the analyses presented here will provide important constrains for asteroseismic modelling.
The bacterial cell wall plays a key role in viability and is an important drug target. The cell wall is made of elongated polymers that are cross-linked to one another to form a load-bearing mesh. An ...alternative cell wall cross-linking mechanism used by the l,d-transpeptidase YcbB has been implicated in the stress-regulated roles of β-lactam resistance, outer membrane defect rescue, and typhoid toxin release. The role for this stress-linked cross-linking in the context of a host infection was unclear. Here, we resolve the crystallographic structures of both
Typhi YcbB and
YcbB acylated with ertapenem that delineate the conserved structural characteristics of YcbB. In parallel, we show that the general involvement of YcbB in peptidoglycan reinforcement under conditions of bacterial outer envelope stress does not play a significant role in acute infections of mice by
and
Typhimurium. Cumulatively, in this work we provide a foundation for the development of novel YcbB-specific antibacterial therapeutics to assist in treatment of increasingly drug-resistant
Typhi infections.
(1) Background: We aimed to analyze the oxidative status of patients with unstable angina pectoris (UA), as well as to determine the correlation of these parameters between coronary arterial and ...peripheral venous blood samples. (2) Methods: The study included 47 human subjects with UA and 45 control subjects. We performed clinical examinations, hemodynamic and coronary angiography measures. Also, in the blood samples, we measured routine laboratory markers and the concentration of pro-oxidants: index of lipid peroxidation (TBARS), superoxide anion radical (O
), hydrogen peroxide (H
O
) and nitrites (NO
), while antioxidant parameters were determined from red blood cells: reduced glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD). All parameters were determined spectrophotometrically. (3) Results: Significantly higher values of TBARS and all measured antioxidants SOD, CAT and GSH were observed in the coronary arterial blood of the UA group relative to coronary arterial blood of the control subjects. On the other hand, in the peripheral venous blood samples, a significantly lower GSH value was found in the UA group compared to the control. (4) Conclusions: This study has shown that the majority of changes in all measured redox markers are found in coronary blood, especially related to the activity of antioxidant components. In patients with an unstable form of angina, prooxidants (superoxide anion radical and index of lipid peroxidation) and endogenous antioxidants (catalase, superoxide dismutase and reduced glutathione) are in direct correlation with the course of ischemic disease. Future studies, where participants would be randomized depending on symptom duration, are necessary to confirm these conclusions.
Context.
Wide hot subdwarf B (sdB) binaries with main-sequence companions are outcomes of stable mass transfer from evolved red giants. The orbits of these binaries show a strong correlation between ...their orbital periods and mass ratios. The origins of this correlation have, so far, been lacking a conclusive explanation.
Aims.
We aim to find a binary evolution model which can explain the observed correlation.
Methods.
Radii of evolved red giants, and hence the resulting orbital periods, strongly depend on their metallicity. We performed a small but statistically significant binary population synthesis study with the binary stellar evolution code MESA. We used a standard model for binary mass loss and a standard metallicity history of the Galaxy. The resulting sdB systems were selected based on the same criteria as was used in observations and then compared with the observed population.
Results.
We have achieved an excellent match to the observed period-mass ratio correlation without explicitly fine-tuning any parameters. Furthermore, our models produce a very good match to the observed period-metallicity correlation. We predict several new correlations, which link the observed sdB binaries to their progenitors, and a correlation between the orbital period, metallicity, and core mass for subdwarfs and young low-mass helium white dwarfs. We also predict that sdB binaries have distinct orbital properties depending on whether they formed in the Galactic bulge, thin or thick disc, or the halo.
Conclusions.
We demonstrate, for the first time, how the metallicity history of the Milky Way is imprinted in the properties of the observed post-mass transfer binaries. We show that Galactic chemical evolution is an important factor in binary population studies of interacting systems containing at least one evolved low-mass (
M
init
< 1.6
M
⊙
) component. Finally, we provide an observationally supported model of mass transfer from low-mass red giants onto main-sequence stars.
The aim of this paper is to emphasize the influence of the magnetization vector misalignment to the magnetic force. In this concrete example the force between permanent ring magnet and soft magnetic ...cylinder was analyzed. Configuration that can be seen in different devices that incorporate permanent magnets. Magnetic force between permanent ring magnet and soft magnetic cylinder is calculated using hybrid boundary element method and semi-analytical approach based on fictitious magnetization charges. By selecting the angle of the magnetization vector inclination, obtained expression can be used for the force calculation of axially or radially magnetized permanent magnet and magnetic body of finite dimension.
In this paper we will present an analysis of the current state of practice in project management in the energy sector in Serbia, wherein the mentioned analysis is based on the results of original ...research on project maturity in this field. This paper includes a literature review about the current situation in the Serbian energy system, the basic concepts and significance of the research of project maturity in organizations, the basic concepts related to the projects in the energy sector, as well as the general state of project management in Serbia. After this, an analysis of the results of the research is presented, which is structured according to the elements of project maturity included in the research, and the methods of analysis. The analysis of the results includes a general analysis as well as statistical and cluster analysis. At the end, based on the analysis results, recommendations for improvements in the area of project management in the energy sector in Serbia are presented.
Choristomas are small aggregates of normal tissue components in aberrant locations, and may be mistaken for true neoplasms. We report a case of an incidentally found mature cartilage island in the ...portal tract of a two-month old piglet. All other examined organs were normal. The pig belonged to the control group of animals in a short-term experiment, was fed with wheat and corn, and did not receive any external or internal treatment. To the best of our knowledge, this is the first case of a mature cartilaginous choristoma in pig liver.
Cyclotron production of 99mTc through the (100)Mo(p,2n)99mTc reaction channel is actively being investigated as an alternative to reactor-based (99)Mo generation by nuclear fission of (235)U. Like ...most radioisotope production methods, cyclotron production of 99mTc will result in creation of unwanted impurities, including Tc and non-Tc isotopes. It is important to measure the amounts of these impurities for release of cyclotron-produced 99mTc (CPTc) for clinical use. Detection of radioactive impurities will rely on measurements of their gamma (γ) emissions. Gamma spectroscopy is not suitable for this purpose because the overwhelming presence of 99mTc and the count-rate limitations of γ spectroscopy systems preclude fast and accurate measurement of small amounts of impurities. In this article we describe a simple and fast method for measuring γ emission rates from radioactive impurities in CPTc. The proposed method is similar to that used to identify (99)Mo breakthrough in generator-produced 99mTc: one dose calibrator (DC) reading of a CPTc source placed in a lead shield is followed by a second reading of the same source in air. Our experimental and theoretical analysis show that the ratio of DC readings in lead to those in air are linearly related to γ emission rates from impurities per MBq of 99mTc over a large range of clinically-relevant production conditions. We show that estimates of the γ emission rates from Tc impurities per MBq of 99mTc can be used to estimate increases in radiation dose (relative to pure 99mTc) to patients injected with CPTc-based radiopharmaceuticals. This enables establishing dosimetry-based clinical-release criteria that can be tested using commercially-available dose calibrators. We show that our approach is highly sensitive to the presence of 93gTc, 93mTc, 94gTc, 94mTc, 95mTc, 95gTc, and 96gTc, in addition to a number of non-Tc impurities.
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