Deciphering the dynamic changes in antibodies against SARS-CoV-2 is essential for understanding the immune response in COVID-19 patients. Here we analyze the laboratory findings of 1,850 patients to ...describe the dynamic changes of the total antibody, spike protein (S)-, receptor-binding domain (RBD)-, and nucleoprotein (N)-specific immunoglobulin M (IgM) and G (IgG) levels during SARS-CoV-2 infection and recovery. The generation of S-, RBD-, and N-specific IgG occurs one week later in patients with severe/critical COVID-19 compared to patients with mild/moderate disease, while S- and RBD-specific IgG levels are 1.5-fold higher in severe/critical patients during hospitalization. The RBD-specific IgG levels are 4-fold higher in older patients than in younger patients during hospitalization. In addition, the S- and RBD-specific IgG levels are 2-fold higher in the recovered patients who are SARS-CoV-2 RNA negative than those who are RNA positive. Lower S-, RBD-, and N-specific IgG levels are associated with a lower lymphocyte percentage, higher neutrophil percentage, and a longer duration of viral shedding. Patients with low antibody levels on discharge might thereby have a high chance of being tested positive for SARS-CoV-2 RNA after recovery. Our study provides important information for COVID-19 diagnosis, treatment, and vaccine development.
Ammonia (NH3) is a promising energy carrier to store and transport renewable hydrogen (H2) that can be generated using, e.g., wind and solar energy. Direct combustion of NH3 is one of the possible ...methods to utilize the energy by the end users. To understand the combustion characteristics of NH3 as a fuel, the laminar burning velocities of NH3/air, NH3/H2/air, NH3/CO/air and NH3/CH4/air premixed flames were investigated experimentally using the heat flux method. Measurements are reported for a wide range of equivalence ratios and blending ratios. Kinetic modeling was also performed using available chemical kinetic mechanisms, namely the GRI-Mech 3.0, the Okafor et al. and the San Diego mechanisms. The experimental results for NH3/air flames agree well with the literature data and it is found that blending NH3 with H2 is the most effective manner to increase the burning velocity of NH3 based fuel mixtures. None of the kinetic mechanisms used can accurately predict most of the measured data. Sensitivity and reaction path analyses indicate that the oxidation of NH3 blended with the additive fuels considered can be understood as the parallel oxidation processes of the individual fuels, and that the source of discrepancy between the experimental and modeling results is related to the inaccuracy of the rate parameters of the N-containing reactions. In this regard, the present detailed and reliable experimental data is of special value for model development and validation.
Oxidative stress is one of the important factors involved in the pathogenesis of idiopathic pulmonary fibrosis (IPF). The equilibrium of Nuclear factor-erythroid-related factor 2 (Nrf2)/BTB ...(broad-complex, tramtrack and bric-a-brac) and CNC (cap'n'collar protein) homology 1, Bach1 determines the expression level of antioxidant factors, further regulating the function of oxidation/antioxidation capacity. Pirfenidone (PFD) is one of two currently for IPF therapy approved drugs. PFD regulates intracellular antioxidants, inhibits secretion of inflammatory cytokines and collagen synthesis. However the mechanisms of its antioxidant effects remain elusive.
Effects of PFD treatment were studied in mouse lung fibroblasts (MLF) following induction by transforming-growth factor beta 1 (TGF-β1) and in mice following bleomycin-induced lung fibrosis. The mRNA and protein levels of oxidative stress-related factors Nrf2/Bach1 and their downstream antioxidant factors heme oxygenase-1 (Ho-1) and glutathione peroxidase 1 (Gpx1) were determined by RT-PCR and Western blot. Fibrosis-related cytokines interleukin-6 (IL-6) and myofibroblast markers type 1 collagen α1 (COL1A1) levels in supernate of MLF, serum, and bronchoalveolar lavage fluid (BALF) as well as malondialdehyde (MDA) in serum and BALF were detected by ELISA, reactive oxygen species (ROS) generation was measured by 2',7'- dichlorofluorescin diacetate (DCFH-DA) assay and lung pathological/morphological alterations in mice were observed by HE and Masson to assess the antioxidant mechanism and therapeutic effects on pulmonary fibrosis induced by bleomycin.
PFD inhibited Bach1 mRNA and protein expressions in mouse lung fibroblasts induced by TGF-β1 and lung tissues with pulmonary fibrosis induced by bleomycin. Furthermore, it improved Nrf2, Ho-1 and Gpx1 mRNA and protein expressions. After PFD treatment, COL1A1and IL-6 levels in supernate of MLF, serum, and BALF as well as ROS in lung tissues and MDA in serum and BALF from a mouse with pulmonary fibrosis were significantly decreased, and the infiltration of lung inflammatory cells and fibrosis degree were alleviated.
Theraputic effects of PFD for IPF were involved in Nrf2/Bach1 equilibrium which regulated the capacity of oxidative stress. The study provided new insights into the antioxidant mechanism of PFD.
A fully-integrated 77 GHz frequency doubling two-path phased-array frequency-modulated continuous-wave (FMCW) transceiver for automotive radar applications is proposed. By utilizing the frequency ...doubling scheme, the chirp bandwidth is improved, and the complexity of the frequency synthesizer and the insertion loss of the local-oscillating (LO) distribution network are both reduced. Top-injected coupled resonator based wide locking range technique is proposed in the frequency doublers to minimize the required injection power to cover the chirp bandwidth plus enough PVT variation margin, and therefore reduce the power consumption of the LO distribution network. Current-reused coupled resonator technique is utilized to implement the LO phase shifting in each receiving path. The digitally controlled artificial dielectric-based transmission lines are inserted in the low noise amplifiers to provide the operation frequency calibration capability. The receiving two-path signals are converted into intermediate frequency by low flicker noise current-mode passive mixers and then combined in the trans-impedance amplifier, followed by the reconfigurable analog baseband processing. Fabricated in 65 nm CMOS, the FMCW transceiver has achieved 1.93 GHz maximum chirp bandwidth, 12.9 ~ 13.2 dBm maximum transmitting power, and 47.8 ~ 100.7 dB programmable receiving conversion gain. The transceiver consumes 343 mW power and 4.64 mm 2 chip area including all of the pads.
This paper presents a CMOS broadband millimeter wave power amplifier (PA) based on magnetically coupled resonator (MCR) matching network. The MCR matching network is analyzed theoretically. Design ...method for MCR-based broadband PA is proposed. For the PA's output matching network, the inductance ratio should be equal to the load/source resistance ratio to achieve broadband impedance transformation. And the coupling coefficient (k) of the MCR can be determined from the no gain ripple condition. Fabricated in 65-nm CMOS process, the PA chip achieves 32.9% peak power added efficiency, 15.3-dBm saturated output power (P sat ), and 12.9-dBm output 1-dB compression point (P1 dB). The fractional bandwidth of the PA is 63.3% from 21.6 to 41.6 GHz, which covers the full Ka-band (26.5 to 40 GHz).
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•The sensing performance of 3DOM WO3 was greatly improved by Pd loading.•The Pd loaded 3DOM WO3 exhibited high response at low working temperature.•The Pd loaded 3DOM WO3 displayed ...fast response and recovery time and good selectivity to hydrogen.•The enhanced hydrogen sensing performance was attributed to the sensitization of Pd loading and the structural defects.
Three-dimensionally ordered macroporous (3DOM) WO3 materials were synthesized via colloidal crystal template method. Pd loading, aimed to improve the sensing performance of the sensor to hydrogen, was carried out by reducing Pd (II) centers. The obtained Pd-loaded 3DOM WO3 samples were carefully characterized by XRD, Raman, SEM, TEM, XPS, UV–vis, Hall and the gas sensing properties targeting ppm level hydrogen were examined. The response of the Pd-loaded 3DOM WO3 to 50 ppm H2 is up to 382 at a low working temperature of 130 °C, which is approximately 225 times higher than that of the pure 3DOM WO3. Additionally, the sensor displays fast response (10 s) and recovery (50 s). More importantly, the Pd-loaded 3DOM WO3 shows superior response to hydrogen with negligible response towards other interfering gases, indicating that the sensor exhibits excellent selectivity to hydrogen. Furthermore, the possible gas sensing mechanism based on the model of depletion layer is proposed to demonstrate that, both the structural defects and the sensitization exerted by Pd are responsible for the outstanding performance in hydrogen sensing.
Epigenetic reprogramming is a critical process of pathological gene induction during cardiac hypertrophy and remodeling, but the underlying regulatory mechanisms remain to be elucidated. Here we ...identified a heart-enriched long noncoding (lnc)RNA, named cardiac-hypertrophy-associated epigenetic regulator (Chaer), which is necessary for the development of cardiac hypertrophy. Mechanistically, Chaer directly interacts with the catalytic subunit of polycomb repressor complex 2 (PRC2). This interaction, which is mediated by a 66-mer motif in Chaer, interferes with PRC2 targeting to genomic loci, thereby inhibiting histone H3 lysine 27 methylation at the promoter regions of genes involved in cardiac hypertrophy. The interaction between Chaer and PRC2 is transiently induced after hormone or stress stimulation in a process involving mammalian target of rapamycin complex 1, and this interaction is a prerequisite for epigenetic reprogramming and induction of genes involved in hypertrophy. Inhibition of Chaer expression in the heart before, but not after, the onset of pressure overload substantially attenuates cardiac hypertrophy and dysfunction. Our study reveals that stress-induced pathological gene activation in the heart requires a previously uncharacterized lncRNA-dependent epigenetic checkpoint.
Combustion of ammonia (NH3) as a carbon-free alternative fuel has been recently widely studied, with vast majority of the burning velocity data obtained at room temperature. In the present study, the ...laminar burning velocity SL of NH3/air mixtures has been measured at unburnt gas temperature Tu from 298 K to 448 K, covering equivalence ratios from 0.85 to 1.25 and at 1 atm using the heat flux method. Kinetic simulations were made with five literature mechanisms developed for NH3 combustion, i.e., Nakamura et al., Otomo et al., San Diego, Okafor et al., and Mei et al. mechanisms, and the influence of radiation heat losses was considered. Using the obtained burning velocity data at different temperatures, the temperature dependence coefficients α in SLSL0=(TuTu0)α were derived, and compared with different models’ predictions. Further analyses of the temperature dependence of SL were carried out through examination of the overall activation energy, temperature and species profiles as well as the reaction paths, and a unique flame structure at the rich side of adiabatic NH3/air flames was found, which resembles ‘over-rich’ phenomena in hydrocarbon flames. At equivalence ratio larger than 1.1 ± 0.05, the NH3/air flames become so rich that (1) the NH2 radical overwhelms the H and OH radicals in maximum mole fraction; (2) after the flame front, H2O converts back to H2 with NO formed at the same time, causing the superadiabatic flame temperature phenomena, i.e. adiabatic flame temperature being lower than the maximum achieved in the flame. Moreover, local minimum NO concentration is found right after the over-rich NH3/air flame front, which may be helpful in reducing NO emissions from NH3 flames in practical applications.
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