Architects and engineers are beginning to consider a new dimension of indoor air: the structure and composition of airborne microbial communities. A first step in this emerging field is to understand ...the forces that shape the diversity of bioaerosols across space and time within the built environment. In an effort to elucidate the relative influences of three likely drivers of indoor bioaerosol diversity – variation in outdoor bioaerosols, ventilation strategy, and occupancy load – we conducted an intensive temporal study of indoor airborne bacterial communities in a high‐traffic university building with a hybrid HVAC (mechanically and naturally ventilated) system. Indoor air communities closely tracked outdoor air communities, but human‐associated bacterial genera were more than twice as abundant in indoor air compared with outdoor air. Ventilation had a demonstrated effect on indoor airborne bacterial community composition; changes in outdoor air communities were detected inside following a time lag associated with differing ventilation strategies relevant to modern building design. Our results indicate that both occupancy patterns and ventilation strategies are important for understanding airborne microbial community dynamics in the built environment.
Abstract Background context Achieving solid implant fixation to osteoporotic bone presents a clinical challenge. New techniques and devices are being designed to increase screw–bone purchase of ...pedicle screws in the lumbar spine via a novel cortical bone trajectory that may improve holding screw strength and minimize loosening. Preliminary clinical evidence suggests that this new trajectory provides screw interference that is equivalent to the more traditionally directed trajectory for lumbar pedicle screws. However, a biomechanical study has not been performed to substantiate the early clinical results. Purpose Evaluate the mechanical competence of lumbar pedicle screws using a more medial-to-lateral path (ie, “cortical bone trajectory”) than the traditionally used path. Study design Human cadaveric biomechanical study. Methods Each vertebral level (L1–L5) was dual-energy X-ray absorptiometry (DXA) scanned and had two pedicle screws inserted. On one side, the traditional medially directed trajectory was drilled and tapped. On the contralateral side, the newly proposed cortical bone trajectory was drilled and tapped. After qCT scanning, screws were inserted into their respective trajectories and pullout and toggle testing ensued. In uniaxial pullout, the pedicle screw was withdrawn vertically from the constrained bone until failure occurred. The contralateral side was tested in the same manner. In screw toggle testing, the vertebral body was rigidly constrained and a longitudinal rod was attached to each screw head. The rod was grasped using a hydraulic grip and a quasi-static, upward displacement was implemented until construct failure. The contralateral pedicle screw was tested in the same manner. Yield pullout (N) and stiffness (N/mm) as well as failure moment (N-m) were compared and bone mineral content and bone density data were correlated with the yield pullout force. Results New cortical trajectory screws demonstrated a 30% increase in uniaxial yield pullout load relative to the traditional pedicle screws (p=0.080), although mixed loading demonstrated equivalency between the two trajectories. No significant difference in construct stiffness was noted between the two screw trajectories in either biomechanical test or were differences in failure moments (p=0.354). Pedicle screw fixation did not appear to depend on bone quality (DXA) yet positive correlations were demonstrated between trajectory and bone density scans (qCT) and pullout force for both pedicle screws. Conclusions The current study demonstrated that the new cortical trajectory and screw design have equivalent pullout and toggle characteristics compared with the traditional trajectory pedicle screw, thus confirming preliminary clinical evidence. The 30% increase in failure load of the cortical trajectory screw in uniaxial pullout and its juxtaposition to higher quality bone justify its use in patients with poor trabecular bone quality.
Biomass burning is a major source of light‐absorbing organic aerosol (brown carbon), but its composition, chemical evolution, and lifetime are not well known. We measured water‐soluble brown carbon ...absorption from 310 to 500 nm on the National Oceanic and Atmospheric Administration Twin Otter aircraft during flights downwind of western United States wildfires in summer 2019. The sampling strategy was near‐Lagrangian and the plume ages spanned 0–5 hr. Trends in brown carbon mass absorption coefficient with plume age varied between flights, and did not show an exponential decay over these short time scales. The measured absorption spectra were smoothly varying, without identifiable contributions from individual chromophores with structured absorption. Using aerosol tracer ions and reference absorption spectra, the calculated contribution of 4‐nitrocatechol to total absorption was <22 ± 9% and <11 ± 5%, although spectral fitting showed that it may be as low as <1.1% and <0.6% at 365 and 405 nm, respectively.
Plain Language Summary
Wildfires are a major source of light‐absorbing particles that affect the Earth's radiative budget, but the lifetime and composition of these particles are not well known. We measured the light absorption and concentration of water‐soluble organic aerosol during aircraft flights through wildfire smoke plumes that were less than 5 hr old. We found that light absorption does not decrease over these short time scales, and may instead increase or remain constant. We examined the absorption spectra and aerosol composition to determine the contribution of individual species to the total absorption by organic aerosol.
Key Points
Dilution‐normalized biomass burning brown carbon showed variable, non‐exponential trends over 5 hr, contrary to recent parameterizations
Spectral fits constrain the contribution of 4‐nitrocatechol to brown carbon absorption to be <1.1% at 365% and <0.6% at 405 nm
Absorption spectra indicate a complex mixture of absorbing compounds without identifiable contributions from individual chromophores
Fungi are ubiquitous in the atmosphere and may play an important role in atmospheric processes. We investigated the composition and diversity of fungal communities over the Amazon rainforest canopy ...and compared these communities to fungal communities found in terrestrial environments. We characterized the total fungal community and the metabolically active portion of the community using high-throughput DNA and RNA sequencing and compared these data to predictions generated by a mass-balance model. We found that the total community was primarily comprised of fungi from the phylum Basidiomycota. In contrast, the active community was primarily composed of members of the phylum Ascomycota and included a high relative abundance of lichen fungi, which were not detected in the total community. The relative abundance of Basidiomycota and Ascomycota in the total and active communities was consistent with our model predictions, suggesting that this result was driven by the relative size and number of spores produced by these groups. When compared to other environments, fungal communities in the atmosphere were most similar to communities found in tropical soils and leaf surfaces. Our results demonstrate that there are significant differences in the composition of the total and active fungal communities in the atmosphere, and that lichen fungi, which have been shown to be efficient ice nucleators, may be abundant members of active atmospheric fungal communities over the forest canopy.
ABSTRACT
The centaur (2060) Chiron exhibits outgassing behaviour and possibly hosts a ring system. On 2011 November 29, Chiron occulted a fairly bright star (R ∼ 15 mag) as observed from the 3-m NASA ...Infrared Telescope Facility (IRTF) on Mauna Kea and the 2-m Faulkes Telescope North (FTN) at Haleakala. Data were taken as visible wavelength images and simultaneous, low-resolution, near-infrared (NIR) spectra. Here, we present a detailed examination of the light-curve features in the optical data and an analysis of the NIR spectra. We place a lower limit on the spherical diameter of Chiron's nucleus of 160.2 ± 1.3 km. Sharp, narrow dips were observed between 280 and 360 km from the centre (depending on event geometry). For a central chord and assumed ring plane, the separated features are 298.5–302 and 308–310.5 km from the nucleus, with normal optical depth ∼0.5–0.9, and a gap of 9.1 ± 1.3 km. These features are similar in equivalent depth to Chariklo's inner ring. The absence of absorbing/scattering material near the nucleus suggests that these sharp dips are more likely to be planar rings than a shell of material. The region of relatively increased transmission is within the 1:2 spin-orbit resonance, consistent with the proposed clearing pattern for a non-axisymmetric nucleus. Characteristics of possible azimuthally incomplete features are presented, which could be transient, as well as a possible shell from ∼900–1500 km: future observations are needed for confirmation. There are no significant features in the NIR light curves, nor any correlation between optical features and NIR spectral slope.
Summary
Bovine respiratory disease (BRD) is considered one of the most economically important diseases in the cattle industry. Ultimately, the selection of cattle that are less susceptible to disease ...will allow producers to reduce the prevalence of BRD and lessen its economic impact. The objective of this study was to validate previously identified loci associated with susceptibility to BRD in an independent population of 140 pre‐weaned Holstein calves from Wisconsin (WI). Using the McGuirk health scoring system, calves were classified as either clinically affected with BRD (n = 35) or healthy (n = 105). Additive genotypic tests were performed for genomic regions previously associated with susceptibility to BRD in calves from California (CA) and New Mexico (NM). Using this method, 4 loci (P < 0.01) consisting of 10 SNP were validated in the WI population, including 2 loci from CA, 1 locus from NM, and 1 locus from a combined CA + NM population. Most of the positional candidate genes and transcription factor binding site motifs associated with these loci have functions related to innate and adaptive immune responses. The validation of loci associated with susceptibility to BRD in independent populations allows producers to more reliably select cattle that are less susceptible to BRD, improving animal welfare, decreasing the annual revenue losses, and lowering the prevalence of the disease.
To determine the clinical utility of the Frontal Assessment Battery (FAB), a short test of frontal lobe functions, in differentiating frontotemporal lobar degeneration (FTLD) from Alzheimer disease ...(AD).
FAB total scores and subscores for 23 subjects with FTLD and 31 subjects with AD were compared for sensitivity, specificity, and positive predictive value. Concurrent validity of the FAB with the Mini-Mental State Examination (MMSE) and other scales was also assessed.
The FAB did not have positive predictive value for FTLD. Total FAB scores did not differ between the FTLD and AD groups. However, three subtests of the FAB (mental flexibility, motor programming, and environmental autonomy) demonstrated significant differences between the two groups. Total FAB scores correlated with scores on the MMSE, a more general test of cognition.
The Frontal Assessment Battery did not discriminate subjects with frontotemporal lobar degeneration from those with Alzheimer disease, though certain subtests may be helpful in differential diagnosis.
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