Understanding organic carbon (OC) sequestration in aggregates and OC stability under different fertilization practices is of key importance in improving soil quality and crop productivity and in ...mitigating the causes of climate change. A long-term field experiment established in 1990 was used to assess the influence of organic and inorganic fertilizers on aggregate-associated OC and its mineralization and on the SOC stock at a soil depth of 0–20cm under an intensive wheat-maize cropping system on Anthrosol in North China. The study involved three treatments: CK, control without fertilization; NPK, nitrogen (N), phosphorus (P) and potassium (K) fertilizers; MNPK, manure (M) combined with N, P and K fertilizers. Soil samples were collected and analyzed to determine the size distribution of aggregates, which were separated by dry sieving; the concentrations of OC and N in aggregates (>2, 0.25–2 and <0.25mm) and bulk soils; and respiration from aggregates and bulk soils in the 0–10 and 10–20cm layers after 21years. Fertilization did not affect the size distribution of aggregates in the surface soil layer, but there was a significant change in the subsurface layer in response to manure addition. Application of NPK and MNPK significantly and evenly augmented OC and N sequestration in the three aggregate classes tested. However, the OC mineralization rate was substantially higher in micro- than in macro-aggregates. Application of NPK and MNPK considerably increased OC mineralization in both aggregates and bulk soils; MNPK yielded the highest values. OC mineralization rates in bulk soils were comparable among the three treatments for the surface layer, but they increased in the subsurface layer because of adding manure. Overall, soils treated for 21years with NPK and MNPK showed increases in the OC pools in the top 20cm of 7.42 and 22.83MgCha−1, respectively, compared with the control treatment, and had average sequestration rates of 353 and 1087kgCha−1yr−1 respectively. Thus, appropriate application of NPK, alone or in combination with manure, can lead to improved OC sequestration by evenly augmenting aggregate-associated OC and providing the same degree of protection for OC in both macro-aggregates and micro-aggregates under a winter wheat-summer maize rotation on Anthrosol.
•Fertilization evenly augmented OC and N sequestration in all aggregates.•OC respiration rate was markedly higher in micro- than in macro-aggregates.•SOC respiration rates in bulk soil were same among treatments at 0–10cm layer.•SOC sequestration rates were 353kgCha−1yr−1 under NPK treatment.•SOC sequestration rates were 1087kgCha−1yr−1 under MNPK treatment.
The veterinary antibiotics contamination in agroecosystems is a substantial problem globally. However, little is known about their toxicity to crops, especially in wheat. This study evaluated the ...phytotoxic effects of the two most representative antibiotics, namely oxytetracycline (OTC) and enrofloxacin (ENR), on seed germination, seedling growth, root elongation and antioxidant status in wheat, and investigated the response of abscisic acid (ABA) to antibiotic stress and its underlying mechanism. The results showed that OTC and ENR under the experimental concentrations (5, 10, 20, 40 and 80 mg·L−1) had no influence on seed germination of wheat. The reduced root length, fresh weight and surface area were observed when the concentrations of OTC and ENR were higher than 10 mg·L−1 and 5 mg·L−1, respectively. High concentrations (＞40 mg·L−1) of antibiotics dramatically decreased the root length, fresh weight, root numbers and surface area as well as the number of stele cells and stele area. The activity of catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD), and malondialdehyde (MDA) content in shoots and roots were increased with the increasing OTC and ENR concentrations. High concentrations (＞40 mg·L−1) of antibiotics improved ABA content and enhanced the transcription levels of genes related to ABA biosynthesis (TaNCED1 and TaNCED2) and metabolism (TaABA8′OH1-A and TaABA8′OH2-A) in shoots and roots of wheat seedlings. Wheat seedlings had relatively strong sensitivity to low concentration (5 mg·L−1) of ENR. These results suggest that OTC and ENR modulate root development and seedling growth by regulating ABA level and antioxidant defense system in wheat.
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•Oxidative stress induced by antibiotics enhances the antioxidant status of wheat.•Wheat seedlings have relatively strong sensitivity to low concentration of ENR.•OTC and ENR at 80 mg·L−1 have no influence on the seed germination of wheat.•OTC and ENR improves ABA content in shoots and roots of wheat seedlings.•Antibiotics enhance the expression of genes related to ABA biosynthesis and metabolism.
We studied changes in the concentrations of aggregate-cementing agents after different reclamation times and with different fertilization regimes, as well as the formation mechanism of aggregates in ...reclaimed soil, to provide a theoretical basis for rapid reclamation of soil fertility in the subsidence area of coal mines in Shanxi Province, China. In this study, soil samples of 0–20 cm depth were collected from four fertilization treatments of a long-term experiment started in 2008: no fertilizer (CK), inorganic fertilizer (NPK), chicken manure compost (M), and 50% inorganic fertilizer plus 50% chicken manure compost (MNPK). The concentrations of cementing agents and changes in soil aggregate size distribution and stability were analysed. The results showed that the formation of >2 mm aggregates, the aggregate mean weight diameter (MWD), and the proportion of >0.25 mm water-stable aggregates (WR0.25) increased significantly after 6 and 11 years of reclamation. The concentration of organic cementing agents tended to increase with reclamation time, whereas free iron oxide (Fed) and free aluminium oxide (Ald) concentrations initially increased but then decreased. In general, the MNPK treatment significantly increased the concentrations of organic cementing agents and CaCO3, and CaCO3 increased by 60.4% at 11 years after reclamation. Additionally, CaCO3 had the greatest effect on the stability of aggregates, promoting the formation of >0.25 mm aggregates and accounting for 54.4% of the variance in the proportion and stability of the aggregates. It was concluded that long-term reclamation is beneficial for improving soil structure. The MNPK treatment was the most effective measure for increasing maize grain yield and concentration of organic cementing agents and CaCO3.
In recent years, silver nanoparticles (AgNPs) have gradually been widely used, especially in the field of anticancer medicine. Ovarian cancer (OC) is the gynaecological malignancy with the highest ...mortality rate, and the current treatment is still based on surgery, chemotherapy and postoperative targeted therapy. Therefore, the development of safe and effective nanoparticles for targeted therapy of OC is very important. This study aimed to prepare a new type of triangular silver nanoparticles (tAgNPs) and evaluate the anticancer properties for OC in vitro and in vivo.
The tAgNPs were chemically synthesized and characterized using scanning electron microscopy (SEM), ultraviolet (UV) spectrophotometry and other techniques. By performing cell-based tests, such as cell counting kit-8 (CCK-8), plate colony formation, cell apoptosis, reactive oxygen species (ROS), and western blot (WB) assays, the inhibitory effects and related mechanisms of tAgNPs on OC cells were analysed.The anticancer effect of tAgNPs in vivo was verified by a SKOV3 tumor-bearing mouse model.
Five types of tAgNPs with different colours were successfully synthesized, with a particle size of 25-50 nm and a good dispersion. The results of in vitro experiments showed that tAgNPs treatment reduced the viability and proliferation of SKOV3 cells, arrested the cell cycle in G0/G1 phase, inhibited the expression levels of proliferation-related factors and cyclins, and promoted cell apoptosis by producing ROS and increasing caspase-3 activity. Consistent with the results of in vitro experiments, in vivo animal experiments also showed that tAgNPs significantly inhibited the proliferation of ovarian cancer. More importantly, no obvious toxic and side effects were observed.
In this study, a novel triangular AgNPs was successfully prepared. tAgNPs are very stable, significantly inhibit the proliferation of OC cells and tumour growth in tumour-bearing mice, providing a promising nanotargeted therapy for OC.
Large-scale distributed renewable energy connected to the rural distribution network has given birth to a new rural distribution system with a high proportion of new energy typical characteristics, ...and the optimal scheduling of the new rural distribution system has become an important issue to ensure the safe and stable operation of the power grid. This paper proposes a method of active-reactive power collaborative optimization scheduling for rural power distribution system with a high proportion of renewable energy. Firstly, the active support capability evaluation model is established, and the active power support capability and reactive power support capability of rural power distribution system are quantitatively evaluated, which provides data basis and boundary conditions for the scheduling part. Then, considering power-loss cost, distribution network operation cost, and penalty cost, a method of active-reactive power collaborative optimization scheduling for rural power distribution systems with a high proportion of renewable energy is proposed. Finally, the active support capability evaluation and regulation platform of the rural power distribution system is built to provide technical support services for the safe and stable operation of the rural power distribution system. Given the problems of overload and overvoltage faced by rural power distribution systems with a high proportion of renewable energy, this paper aims to solve the key technical challenges of optimization and regulation of new rural power distribution systems. The results show that the optimized control method proposed in this paper has better security and economy, and is conducive to promoting the construction and operation of the new rural power distribution system.
Ion-cutting of piezoelectric LiNbO3 (LN) thin film provides a material platform for the design and fabrication of novel integrated photonics and RF MEMS devices. In this paper, the ion-slicing ...mechanisms of He-implanted LN with different orientations are investigated. The anisotropy of film exfoliation is observed on LN wafers with different orientations. The Z-cut LN shows regular surface blistering and “plate-like” exfoliation, while the Y-cut LN shows the unique “rolled-up” exfoliation. Two types of defect, i.e. the pressure-related plateau defect and the stress-related crack defect, are observed to contribute to the film exfoliation. Moreover, the defect evolution in H-implanted LN is investigated. In comparison with the He-implanted LN, implanted H ions are mainly trapped by O-H bond and the implantation-induced strain is not strong enough, which are inadequate to form the continuous crack. Therefore the H ions are not favorable for the mass production of LNOI substrates.
Water pollution is a major global challenge given the increasing growth in industry and human population, and certain metals can be highly toxic and contribute to this significantly. In this study, ...polyvinylpyrrolidone-coated magnetic nanoparticles (PVP-Fe
3
O
4
NPs) were used to remove metals (Cd, Cr, Ni, and Pb) from synthetic soft water and sea water in the presence and absence of fulvic acid. Nanoparticle (NP) suspensions were added to water media at a range of metal concentrations (0.1-100 mg L
−1
). Removal at different time points (1.5, 3, 6, 12, 24 hours) was also evaluated. Results showed that 167 mg L
−1
PVP-Fe
3
O
4
NPs could remove nearly 100% of four metals at 0.1 mg L
−1
and more than 80% at 1 mg L
−1
. The removal decreased as the initial metal concentration increased, although essentially 100% of the Pb was removed under all conditions. The kinetic adsorption fitted well to the pseudo-second-order model and in general, the majority of metal adsorption occurred within the first 1.5 hours. These NPs are a reliable method to remove metals under a wide range of environmentally relevant conditions. Our previous research showed the NPs effectively removed oil from waters, so these NPs offer the possibility of combined
in situ
remediation of oil and metals.
PVP-Fe
3
O
4
NPs synthesized with no organic solvents, low toxicity reactants and low temperature/energy requirements could remove Cd, Cr, Ni, Pb efficiently in the different synthetic water media under environmentally relevant conditions.
•Splitting N in 3–4 applications improved wheat yield by 7 % and grain protein content by 5 %.•The effect of split N on yield was larger for winter wheat than for spring wheat.•Split N mitigated the ...negative relationship between yield and grain protein content.
Management of nitrogen (N) fertilizer is critical to avoid the environmental consequences of both over-fertilization and excess reactive N in the system, and chronic under-fertilization and soil mining. Split N application seeks to improve nitrogen use efficiency in both small-holdings and large-scale mechanized farms, but lack of conclusive evidence on its benefits restricts the adoption of this practice. Here we report a global meta-analysis of split N application in wheat using 129 studies with 2661 comparisons. Split N in 3–4 applications improved yield by 7.0 % and grain protein by 5.2 % in relation to single application. The effect of split N on yield was larger for winter wheat than for spring wheat, while split N improved grain protein in both winter and spring wheat. Its effects on both yield and grain protein were larger under conventional tillage, in humid climate especially for winter wheat, in coarse textured soils, on less fertile soils, and at high N rate. Splitting N in 3–4 applications would increase annual production of winter wheat by 12.9 M t grain and 1.3 M t protein globally. By identifying the conditions where split N application is more likely to deliver agronomic benefits, our study would contribute to the targeted adoption of this practice.
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