The line structured light plane calibration method using a plane target cannot produce satisfactory calibration results due to inaccurate positioning of the calibrated points. Field of view noise and ...sensor noise affect the target light stripe extraction and camera parameter calculation during the calibration process. These factors will cause the calculation of the coordinates of the calibrated point to deviate, and thus affect the light plane calibration. To solve this problem, we propose a new method to calculate the calibrated point based on spatial geometry. Firstly, for the projection line corresponding to the feature point on the light stripe and the corresponding line on the target, a common perpendicular of these two lines above is established, and since the sum of the squares of the distances from the midpoint to the two straight lines is the smallest, the midpoint of the common perpendicular is taken as the calibrated point. Secondly, the target is moved to different positions, and the non-collinear calibrated points are calculated. Finally, the parameters of the light plane are obtained by fitting these calibrated points. This method requires only a checkerboard target, and has a simple calibration process. The experimental results show that the average error of the calibration method proposed in this paper is 0.011 mm, which is less than the 0.031 mm of the calibration method based on the plane target with cross-ratio invariant.
High-speed steel is widely used for cutting tools due to its convenience of preparation and cost-effectiveness. Previous research has shown that deep cryogenic treatments improve the mechanical ...properties of high-speed steel, due to the transformation of the residual austenite and the precipitation of carbide, while few studies have researched martensitic changes. The variations in martensite multi-level microstructures in AISI M35 high-speed steel, treated over different deep cryogenic time periods, were investigated in this study. Meanwhile, the effect of these variations on the mechanical properties of the selected steel was discussed. It was found that prolonging deep cryogenic time facilitated an increase in dislocation, low-angle grain boundary, and the coincident-site lattice boundary (especially the twin boundary) of martensite. The size of the martensite block (db) and lath (dl) decreased with deep cryogenic time. However, the effect on the microstructure was limited when the cryogenic treatment time exceeded 5 h. The increase in dislocation decreased the temperature for carbide precipitation and promoted fine carbide precipitation during tempering. The refinement of martensite multi-level microstructures and the greater precipitation of fine carbides gave the tempered specimens excellent impact toughness. The impact toughness of the tempered samples undergoing deep cryogenic treatment for more than 5 h was about 32% higher than the sample without deep cryogenic treatment.
In response to the real-time imaging detection requirements of structural defects in the R region of rib-stiffened wing skin, a defect detection algorithm based on phased-array ultrasonic imaging for ...wing skin with stiffener is proposed. We select the full-matrix-full-focusing algorithm with the best imaging quality as the prototype for the required detection algorithm. To address the problem of poor real-time performance of the algorithm, a sparsity-based full-focusing algorithm with symmetry redundancy imaging mode is proposed. To address noise artifacts, an adaptive beamforming method and an equal-acoustic-path echo dynamic removal scheme are proposed to adaptively suppress noise artifacts. Finally, within 0.5 s of imaging time, the algorithm achieves a detection sensitivity of 1 mm and a resolution of 0.5 mm within a single-frame imaging range of 30 mm × 30 mm. The defect detection algorithm proposed in this paper combines phased-array ultrasonic technology and post-processing imaging technology to improve the real-time performance and noise artifact suppression of ultrasound imaging algorithms based on engineering applications. Compared with traditional single-element ultrasonic detection technology, phased-array detection technology based on post-processing algorithms has better defect detection and imaging characterization performance and is suitable for R-region structural detection scenarios.
In this paper, a novel method for the effective extraction of the light stripes in rail images is proposed. First, a preprocessing procedure that includes self-adaptive threshold segmentation and ...brightness enhancement is adopted to improve the quality of the rail image. Secondly, center of mass is utilized to detect the center point of each row of the image. Then, to speed up the procedure of centerline optimization, the detected center-points are segmented into several parts based on the geometry of the rail profile. Finally, piecewise fitting is adopted to obtain a smooth and robust centerline. The performance of this method is analyzed in detail, and experimental results show that the proposed method works well for rail images.
Deep cryogenic treatment applied in high-speed steel yielded promising results, and it was found that tempering played a vital role. This paper systematically studied the effect of tempering ...temperatures on the microstructural composition (residual austenite, martensite, and carbides) and mechanical properties (Vickers hardness and impact toughness) of AISI M35 high-speed steel subjected to deep cryogenic treatment. An increase in the tempering temperatures facilitated the transformation of residual austenite and the formation of martensite blocks corresponding to a decrease in the number of dislocations. Moreover, the carbide precipitation (secondary carbides and nanoscale carbides) began at a tempering temperature of 350 °C, increased at 450 °C, and reached its maximum at 550 °C. The fracture mechanism on the micro-level could be interpreted as follows: cracks occurred in the carbides with larger sizes (primary carbides and large secondary carbides) and at the carbide/matrix interface, and small secondary carbides decohered at the interface, forming microvoids and facilitating plastic deformation. In addition, the specimen tempered at 150 °C exhibited the highest hardness of 880.4 HV1 due to the highest number of dislocations. The impact toughness of the sample tempered at 550 °C was the best, namely 2.50 MJ m−2, due to an increase in the number of martensite block boundaries and the more homogeneous carbide precipitation.
Features determined from keypoints or edges are widely used in multi-source image matching. However, for cases that exhibit non-linear intensity changes and significant noise, identifying sufficient ...identical features for multi-source image pairs can be complicated. Motivated by problems encountered in existing multi-source image matching algorithms, a robust and effective algorithm for multi-source image matching was proposed in this paper. First, keypoints uniformly and sufficiently distributed on the significant edge were extracted by a location-based boosting detector. Second, in order to effectively describe the corresponding region and reduce the influence of noise and non-linear intensity changes, a novel descriptor, denoted as the histogram of point-edge orientation (HPEO) was proposed for multi-source image matching. A bilateral matching process was then used to remove the incorrect matches. Experiments were performed with standard infrared-visible datasets and the results demonstrate that the proposed algorithm achieves a more accurate matching performance.
Epithelial-mesenchymal transition (EMT) has been associated with wound healing, tumorigenesis, and metastasis. Circular RNAs (circRNAs) are functional non-coding RNAs involved in multiple human ...cancers. However, whether and how circRNAs contribute to the EMT in hepatocellular carcinomas (HCC) remains to be deciphered. In this study, we investigated the regulation and function of hsa_circ_0003288 on programmed death-1 ligand 1 (PD-L1) during EMT and HCC invasiveness.
Hsa_circ_0003288 expression was measured by real-time quantitative reverse transcriptase PCR (qRT-PCR). Luciferase reporter assays, RNA pull-down assay and fluorescence in situ hybridization (FISH) were used to determine the correlation between hsa_circ_0003288 and miR-145 and between miR-145 and PD-L1. Furthermore, ectopic overexpression and siRNA-mediated downregulation of hsa_circ_0003288, transwell assays, and in vivo studies were used to determine the function of hsa_circ_0003288 on the EMT and invasiveness of L02 and HCC cells.
miR-145 directly targeted the PD-L1 3'-untranslated region (UTR) region, and hsa_circ_0003288 acted as a miR-145 sponge to regulate PD-L1 expression. Overexpression of hsa_circ_0003288 increased PD-L1 levels and promoted EMT, migration, and invasiveness of L02 cells. These observations were reversed after knockdown of hsa_circ_0003288 in HepG2 and Huh7 cells. Overexpression of PD-L1 rescued EMT, migration, and invasiveness of HepG2 and Huh7 cells after knockdown of hsa_circ_0003288. Furthermore, hsa_circ_0003288 knockdown reduced EMT in in vivo studies. Hsa_circ_0003288/PD-L1 axis was found to mediate the metastatic phenotypes via the PI3K/Akt pathway in HCC. Additionally, expression levels of hsa_circ_0003288 were increased and positively correlated with PD-L1 expression in HCC tissues.
Our findings demonstrated that hsa_circ_0003288 promoted EMT and invasion of HCC via the hsa_circ_0003288/miR-145/PD-L1 axis through the PI3K/Akt pathway. Targeting hsa_circ_0003288 may be a therapeutic strategy for the treatment of HCC.
The quality inspection of solder joints on aviation plugs is extremely important in modern manufacturing industries. However, this task is still mostly performed by skilled workers after welding ...operations, posing the problems of subjective judgment and low efficiency. To address these issues, an accurate and automated detection system using fine-tuned YOLOv5 models is developed in this paper. Firstly, we design an intelligent image acquisition system to obtain the high-resolution image of each solder joint automatically. Then, a two-phase approach is proposed for fast and accurate weld quality detection. In the first phase, a fine-tuned YOLOv5 model is applied to extract the region of interest (ROI), i.e., the row of solder joints to be inspected, within the whole image. With the sliding platform, the ROI is automatically moved to the center of the image to enhance its imaging clarity. Subsequently, another fine-tuned YOLOv5 model takes this adjusted ROI as input and realizes quality assessment. Finally, a concise and easy-to-use GUI has been designed and deployed in real production lines. Experimental results in the actual production line show that the proposed method can achieve a detection accuracy of more than 97.5% with a detection speed of about 0.1 s, which meets the needs of actual production
The effect of porosity and pore size on the quasi-static compression properties and energy absorption characteristics of the steel foam was investigated in this paper. The 316L steel foams were ...prepared through powder metallurgy using urea as the space holder. The macrostructure of steel foam and microstructure of the pore walls were characterized, and the quasi-static compression experiments were conducted on the specimens in the axial direction at a strain rate of 10
s
. The results show that the increase in porosity decreases the yield strength and plastic modulus of the steel foam but increases the densification strain of the steel foam. The yield strength of the steel foam decreases significantly when the pore size is 2.37 mm. However, the pore size has little effect on the plastic modulus. Moreover, the energy absorption per volume of the steel foam decreases with increasing porosity at the same strain. The effect of porosity on energy absorption efficiency is greater than that of pore size.
In this study, slow strain rate tensile tests under in situ hydrogen electrochemical charge are performed to investigate the hydrogen embrittlement (HE) behavior of selective laser-melted (SLM) and ...post-heat-treated Inconel 718 alloys in tandem with microstructure evolution and cracking feature characterization. The results indicate that the high density of dislocations and local strain along the cellular structure walls in the as-SLM sample increase local hydrogen concentration, leading to a crack along the cell walls of the cellular structure. The tensile strength and the HE susceptibility are significantly enhanced by the precipitation of the γ′, γ″, and δ phases generated by solution aging. Solution-aged samples suffer from the severe HE susceptibility caused by the δ phase formation along the grain boundaries and the cell walls, which lead to the δ/γ interface cracking.