The optical navigation camera telescope (ONC-T) is a telescopic framing camera with seven colors onboard the
Hayabusa2
spacecraft launched on December 3, 2014. The main objectives of this instrument ...are to optically navigate the spacecraft to asteroid Ryugu and to conduct multi-band mapping the asteroid. We conducted performance tests of the instrument before its installation on the spacecraft. We evaluated the dark current and bias level, obtained data on the dependency of the dark current on the temperature of the charge-coupled device (CCD). The bias level depends strongly on the temperature of the electronics package but only weakly on the CCD temperature. The dark-reference data, which is obtained simultaneously with observation data, can be used for estimation of the dark current and bias level. A long front hood is used for ONC-T to reduce the stray light at the expense of flatness in the peripheral area of the field of view (FOV). The central area in FOV has a flat sensitivity, and the limb darkening has been measured with an integrating sphere. The ONC-T has a wheel with seven bandpass filters and a panchromatic glass window. We measured the spectral sensitivity using an integrating sphere and obtained the sensitivity of all the pixels. We also measured the point-spread function using a star simulator. Measurement results indicate that the full width at half maximum is less than two pixels for all the bandpass filters and in the temperature range expected in the mission phase except for short periods of time during touchdowns.
Different regions of the striatum regulate different types of behavior. However, how dopamine signals differ across striatal regions and how dopamine regulates different behaviors remain unclear. ...Here, we compared dopamine axon activity in the ventral, dorsomedial, and dorsolateral striatum, while mice performed a perceptual and value-based decision task. Surprisingly, dopamine axon activity was similar across all three areas. At a glance, the activity multiplexed different variables such as stimulus-associated values, confidence, and reward feedback at different phases of the task. Our modeling demonstrates, however, that these modulations can be inclusively explained by moment-by-moment
in the expected reward, that is the temporal difference error. A major difference between areas was the overall activity level of reward responses: reward responses in dorsolateral striatum were positively shifted, lacking inhibitory responses to negative prediction errors. The differences in dopamine signals put specific constraints on the properties of behaviors controlled by dopamine in these regions.
Programmed cell death (PCD) is an integral part of plant development and of responses to abiotic stress or pathogens. Although the morphology of plant PCD is, in some cases, well characterised and ...molecular mechanisms controlling plant PCD are beginning to emerge, there is still confusion about the classification of PCD in plants. Here we suggest a classification based on morphological criteria. According to this classification, the use of the term 'apoptosis' is not justified in plants, but at least two classes of PCD can be distinguished: vacuolar cell death and necrosis. During vacuolar cell death, the cell contents are removed by a combination of autophagy-like process and release of hydrolases from collapsed lytic vacuoles. Necrosis is characterised by early rupture of the plasma membrane, shrinkage of the protoplast and absence of vacuolar cell death features. Vacuolar cell death is common during tissue and organ formation and elimination, whereas necrosis is typically found under abiotic stress. Some examples of plant PCD cannot be ascribed to either major class and are therefore classified as separate modalities. These are PCD associated with the hypersensitive response to biotrophic pathogens, which can express features of both necrosis and vacuolar cell death, PCD in starchy cereal endosperm and during self-incompatibility. The present classification is not static, but will be subject to further revision, especially when specific biochemical pathways are better defined.
Interactions between cells and the extracellular matrix are at the core of tissue engineering and biology. However, most studies of these interactions have used traditional two-dimensional (2D) ...tissue culture, which is less physiological than three-dimensional (3D) tissue culture. In this study, we compared cell behavior in four types of commonly used extracellular matrix under 2D and 3D conditions. Specifically, we quantified parameters of cell adhesion and migration by human foreskin fibroblasts in cell-derived matrix or hydrogels of collagen type I, fibrin, or basement membrane extract (BME). Fibroblasts in 3D were more spindle shaped with fewer lateral protrusions and substantially reduced actin stress fibers than on 2D matrices; cells failed to spread in 3D BME. Cell-matrix adhesion structures were detected in all matrices. Although the shapes of these cell adhesions differed, the total area per cell occupied by cell-matrix adhesions in 2D and 3D was nearly identical. Fibroblasts migrated most rapidly in cell-derived 3D matrix and collagen and migrated minimally in BME, with highest migration directionality in cell-derived matrix. This identification of quantitative differences in cellular responses to different matrix composition and dimensionality should help guide the development of customized 3D tissue culture and matrix scaffolds for tissue engineering.
The development of highly active and reusable supported catalysts for Suzuki‐Miyaura coupling and catalytic C−H arylation is important for fundamental and applied chemistry, with these reactions ...being used to produce medical compounds and functional materials. Herein, we found that a mesoporous composite made of a linear poly(4‐vinylpyridine) and tetrachloropalladate acted as a dual‐mode catalyst for a variety of cross‐coupling reactions, with both Pd nanoparticles and a Pd complex catalyst being observed under different conditions. The polyvinylpyridine‐palladium composite 1 was readily prepared via the molecular convolution of poly(4‐vinylpyridine) and sodium tetrachloropalladate to provide a hardly soluble polymer‐metal composite. The Suzuki‐Miyaura coupling and the C−H arylation of aryl chlorides and bromides with arylboronic acids, thiophenes, furans, benzene, and anisole proceeded in the presence of 0.004 mol% (40 mol ppm) to 1 mol% Pd of 1 to afford the corresponding coupling products in high yields. Furthermore, the catalyst was reused without an appreciable loss of activity. Pharmaceutical compounds and functional materials were synthesized via the coupling reactions. N2 gas adsorption/desorption analysis indicated that the catalyst had a mesoporous nature, which played a crucial role in the catalysis. In the Suzuki‐Miyaura couplings, in situ generated palladium nanoparticles in the polymer matrix were catalytically active, while a polymeric Pd(II) complex was crucial in the C−H arylations. These catalytic species were investigated via XAFS, XPS, far‐infrared absorption, and Raman spectroscopies, as well as DFT calculations.
We have discovered that basement membrane and its major components can induce rapid, strikingly robust fibronectin organization. In this new matrix assembly mechanism, α5β1 integrin-based focal ...adhesions slide actively on the underlying matrix toward the ventral cell center through the dynamic shortening of myosin IIA-associated actin stress fibers to drive rapid fibronectin fibrillogenesis distal to the adhesion. This mechanism contrasts with classical fibronectin assembly based on stable or fixed-position focal adhesions containing αVβ3 integrins plus α5β1 integrin translocation into proximal fibrillar adhesions. On basement membrane components, these sliding focal adhesions contain standard focal adhesion constituents but completely lack classical αVβ3 integrins. Instead, peripheral α3β1 or α2β1 adhesions mediate initial cell attachment but over time are switched to α5β1 integrin-based sliding focal adhesions to assemble fibronectin matrix. This basement-membrane-triggered mechanism produces rapid fibronectin fibrillogenesis, providing a mechanistic explanation for the well-known widespread accumulation of fibronectin at many organ basement membranes.
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•Basement membrane matrix molecules can rapidly induce robust fibronectin assembly•A contractile actomyosin winch drives focal adhesion sliding and matrix assembly•Integrin switching mediates focal adhesion dynamics and fibronectin matrix assembly
Lu et al. report that basement membranes can induce robust cellular fibronectin assembly using a contractile actomyosin winch, pulling on sliding focal adhesions to generate abundant fibronectin fibrils. This process involves integrin switching and can help to explain the widespread accumulation of fibronectin at many organ basement membranes.
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