To investigate the division, proliferation and differentiation abilities of nestin+/GFAP+ cell after spinal cord injury and to identify whether it has the characteristic of neural stem cells (NSCs).
...Twelve male SD rats, aged 8 weeks and weighing 200-250 g, were randomized into 2 groups (n=6 per group): model group in which the spinal cord injury model was established by aneurysm clip compression method, and control group in which no processing was conducted. At 5 days after modeling, T8 spinal cord segment of rats in each group were obtained and the gray and the white substance of spinal cord outside the ependymal region around central tube were isolated to prepare single cell suspension. Serum-free NSCs culture medium was adopted to culture and serum NSCs culture medium was applied to induce differentiation. Immunohistochemistry detection and flow cytometry were applied to observe and analyze the type of cells and their capability of division, proliferation and differentiation.
At 3-7 days after injury, the
The present study aimed to analyze the indications, feasibility, safety and clinical effects of total spondylectomy and spine reconstruction through posterior or combined anterior-posterior ...approaches for thoracic lumbar and sacral vertebrae tumors. Between December 2009 and May 2012, 10 patients with thoracic lumbar and sacral vertebrae tumors were retrospectively analyzed. Different surgical indications and approaches were used according to the affected segments, the extent of lesion involvement and the specific pathology results. One-stage posterior or combined anterior-posterior total spondylectomy and reconstruction was used for the treatment of complicated thoracic lumbar and sacral vertebral malignant tumors and invasive benign tumors. The duration of surgery, levels of intraoperative blood loss and transfusions, and the clinical effects were observed. The average surgical duration was 6.8 h (range, 4.8-12 h), with an average blood loss level of 3,200 ml (range, 1,500-10,000 ml) and an average transfusion level of 2,500 ml. During the average 15 months (range, 3-29 months) follow up, two patients succumbed and one patient experienced tumor recurrence. Neither tumor reoccurrence nor metastasis was observed in all other patients. Personalized surgical indications and approaches according to the affected segments, the extent of lesion involvement and the specific pathology results would aid in the reduction of pain, the improvement of nerve function and the reduction of tumor recurrence.
The aim of the present study was to determine whether hyaluronic acid (HA) or physical therapy agents (PTA) can improve functional parameters in patients with knee Kashin-Beck disease (KBD). For 2 ...years, patients (n=55) were treated with HA weekly for 5 weeks, then received 6th and 7th injections on the 3rd and 6th month, respectively, for 7 injections in total. Patients (n=53) were treated with PTA five times a week for 3 weeks every month for 6 months. The patients were evaluated with the Western Ontario and McMaster University Osteoarthritis Index (WOMAC) and the visual analog pain scale (VAS). Trial registration, ChiCTR-TRC-12002189 (http://www.chictr.org/). During the study, following treatment interruption, pain increased in the PTA group (from a mean value of 85.7±83.8 mm at month 12 to 145.2±128.8 mm at month 18 and 201.3±150.5 mm at month 24), while it remained stable in the HA group (from a mean value of 80.7±70.6 mm at month 12 to 90.1±95.2 mm at month 18 and 82.6±85.3 mm at month 24), with a statistically significant difference in favor of HA at month 18 (P<0.05) and month 24 (P<0.05). Joint stiffness, physical function and total WOMAC showed the same trend as pain. The global efficacy judgments by the patients and the investigators showed a statistically significant difference in favor of HA at month 18 (P<0.05) and month 24 (P<0.05). In conclusion, although all the patients improved in terms of pain and function, HA was superior to PTA alone for pain relief and lasting effect.
Background The aim of our study was to evaluate the clinical efficacy of posterior vs. anterior instrumentation for the treatment of spinal tuberculosis in adults. Methods The electronic databases ...such as PubMed, MEDLINE, Springer, EMBASE, Google scholar, and Cochrane library were searched to select the potentially relevant reports that compared the efficacy of posterior instrumentation group (group A) with anterior instrumentation group (group B) in the treatment of spinal tuberculosis. Outcome assessments were correction of angle, loss of correction, fusion rate of the grafting bone, and complications after surgery. Results This meta-analysis included four trials published between 2006 and 2012, involving 291 adult patients (group A, 154; group B, 137) with spinal tuberculosis. The overall meta-analysis showed that there were no significant differences (P > 0.01) between group A and group B in correction of angle and loss of correction at final follow-up after operation The pooled WMD (weighted mean difference) of group A and group B was 2.85 (95% CI (confidence interval) = -1.25 ~ 6.94) and 1.14 (95% CI = -3.07 ~ 5.34), respectively. Besides, no significant differences (P > 0.01) were observed in fusion rate of the grafting bone and complications after operation between group A and group B, and the pooled ORs (odds ratio) were 0.65 (95% CI = -0.23 ~ 1.85) and (95% CI = -0.19 ~ 1.50), respectively. Conclusions Our results suggested that the posterior instrumentation appeared to have the same clinical outcome with the anterior instrumentation in the treatment of the adult patients with spinal tuberculosis. Keywords: Adult spinal tuberculosis, Thoracic and lumbar, Meta-analysis
This study tested the biomechanics of artificial atlantoodontoid joint replacement (AAOJR) in a dog model. Dogs were divided into the artificial AAOJR group (n=10), the decompression group (n=10), ...and the healthy control group (n=10) using a random number table.
To evaluate whether the use of AAOJR for repair of atlantoaxial instability retains rotation and restores stability.
Atlantoaxial instability is characterized by excessive movement or laxity at the junction between the atlas (C1) and axis (C2). Pure decompression can lead to considerable loss of head and neck rotation and postoperative impairment. A series of biomechanical tests on cadavers found that the artificial AAOJR might rebuild the stability and retain the rotation function.
We designed the AAOJ based on the radiologic and anatomic data of the dog atlas and axis, and established an animal model by resecting the odontoid and implanting the AAOJ into dogs. The biomechanical experiments measured the range of motion (ROM), neutral zone (NZ), and stiffness of flexion, extension, lateral bending, and axial rotation in the intact state, the decompressed state, after AAOJR, and after a fatigue test.
Compared with the intact state, after decompression operation, ROM and NZ in all directions, and stiffness during flexion were increased, and stiffness in all other directions was decreased. Compared with the after decompression state, AAOJR before and after the fatigue test resulted in decreased ROM in all directions (all P<0.05), decreased NZ during flexion/extension and lateral bending (all P<0.05), an increased NZ during axial rotation (both P<0.05), and increased stiffness in all directions (all P<0.05).
These results indicate that AAOJR could reconstruct the vertebral stability of the C1-C2 segment and retain some axial rotation function.
To explore the expression of nestin and glial fibrillary acidic protein (GFAP) at different time and sites after spinal cord injury in adult rats.
Seventy-two adult Sprague-Dawley rats, aging 8 weeks ...and weighing from 180 to 220 g, were randomly divided into 11 experimental groups (66, n=6) and 1 control group (n= 6). In the experimental groups, the rat spinal cord injury models were established by aneurysm clip compression, and the expression and proliferation of nestin and GFAP at different time (1 day, 3 days, 5 days, 1 week, 2 weeks, 3 weeks, weeks, 5 weeks, 6 weeks, 7 weeks and 8 weeks) and at different sites (injured site and adjacent site) were observed with toluidine blue staining, immunofluorescent staining and the analytical system of photographs. In control group, the same site of the rat spinal cord was exposed without aneurysm clip compression. The same preparation and examination were done as the experimental groups.
Toluidine blue staining results showed that contour of neurite and pericaryon w
碩士
國立成功大學
航空太空工程學系碩博士班
98
This study focuses on the temperature effects on tensile and compressive behavior of the single-walled open-ended carbon nanocones. A simulation system model was built by ...using software based on the molecular dynamics simulation method. This study uses the Tersoff potential function to simulate the interaction between carbon atoms. Single-walled open-ended carbon nanocones with the apex angle of 19.2 degree were illustrated.
This study mainly includes three items as follows:
1. To investigate the temperature effect on the tensile and compressive behavior of the single-walled open-ended carbon nanocone.
2. To inspect the length effect on the tensile and compressive behavior of the single-walled open-ended carbon nanocone.
3. To examine the apex angle effect on the tensile and compressive behavior of the single-walled open-ended carbon nanocone.
The simulation results tell us that, for the tensile behavior of a single-walled open-ended carbon nanocone with the geometry parameter fixed,
Forty-eight New Zealand rabbits were divided into normal group (n=18), control group (n=18) and Chinese herbs treatment group (n=12) randomly. The rabbits in the normal group received sham-operation, ...and the OA model was established by Hulth's method. All the rabbits in the treatment group were given bushen huoxue decoction from the 6th week after the operation. At 6th, 8th and 12th week after the operation, the NO concentrations of the serum, joint cartilage and synovium were examined.
Indicated that the NO concentrations of the serum, joint cartilage and synovium in the control group were all significantly higher than those in the normal group, with the joint cartilage more obvious (P<0.05). In the Chinese herbs treatment group the NO concentrations in all the parts obviously decreased as compared with the control group (P<0.05). It is suggested that bushen huoxue decoction decrease the levels of NO in the serum, synovium and joint cartilage in the OA rabbit.
The nodal regulatory properties of microRNAs (miRNA) in metastatic cancer may offer new targets for therapeutic control. Here, we report that upregulation of miR-483-5p is correlated with the ...progression of human lung adenocarcinoma. miR-483-5p promotes the epithelial-mesenchymal transition (EMT) accompanied by invasive and metastatic properties of lung adenocarcinoma. Mechanistically, miR-483-5p is activated by the WNT/β-catenin signaling pathway and exerts its prometastatic function by directly targeting the Rho GDP dissociation inhibitor alpha (RhoGDI1) and activated leukocyte cell adhesion molecule (ALCAM), two putative metastasis suppressors. Furthermore, we found that downregulation of RhoGDI1 enhances expression of Snail, thereby promoting EMT. Importantly, miR-483-5p levels are positively correlated with β-catenin expression, but are negatively correlated with the levels of RhoGDI1 and ALCAM in human lung adenocarcinoma. Our findings reveal that miR-483-5p is a critical β-catenin-activated prometastatic miRNA and a negative regulator of the metastasis suppressors RhoGDI1 and ALCAM.
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