Self-assembling peptides are biomedical materials with unique structures that are formed in response to various environmental conditions. Governed by their physicochemical characteristics, the ...peptides can form a variety of structures with greater reactivity than conventional non-biological materials. The structural divergence of self-assembling peptides allows for various functional possibilities; when assembled, they can be used as scaffolds for cell and tissue regeneration, and vehicles for drug delivery, conferring controlled release, stability, and targeting, and avoiding side effects of drugs. These peptides can also be used as drugs themselves. In this review, we describe the basic structure and characteristics of self-assembling peptides and the various factors that affect the formation of peptide-based structures. We also summarize the applications of self-assembling peptides in the treatment of various diseases, including cancer. Furthermore, the in-cell self-assembly of peptides, termed reverse self-assembly, is discussed as a novel paradigm for self-assembling peptide-based nanovehicles and nanomedicines.
Prion diseases are neurodegenerative disorders in humans and animals for which no therapies are currently available. Here, we report that
Valeton (Zingiberaceae) (
) extract was partly effective in ...decreasing prion aggregation and propagation in both in vitro and in vivo models.
extract inhibited self-aggregation of recombinant prion protein (PrP) in a test tube assay and decreased the accumulation of scrapie PrP (PrP
) in ScN2a cells, a cultured neuroblastoma cell line with chronic prion infection, in a concentration-dependent manner.
extract also modified the course of the disease in mice inoculated with mouse-adapted scrapie prions, completely preventing the onset of prion disease in three of eight mice. Biochemical and neuropathological analyses revealed a statistically significant reduction in PrP
accumulation, spongiosis, astrogliosis, and microglia activation in the brains of mice that avoided disease onset. Furthermore, PrP
accumulation in the spleen of mice was also reduced.
extract precluded prion infection in cultured cells as demonstrated by the modified standard scrapie cell assay. This study suggests that
extract could contribute to investigating the modulation of prion propagation.
Prions are infectious protein particles known to cause prion diseases. The biochemical entity of the pathogen is the misfolded prion protein (PrP
Sc
) that forms insoluble amyloids to impair brain ...function. PrP
Sc
interacts with the non-pathogenic, cellular prion protein (PrP
C
) and facilitates conversion into a nascent misfolded isoform. Several small molecules have been reported to inhibit the aggregation of PrP
Sc
but no pharmacological intervention was well established thus far. We, here, report that acylthiosemicarbazides inhibit the prion aggregation. Compounds 7x and 7y showed almost perfect inhibition (EC
50
= 5 µM) in prion aggregation formation assay. The activity was further confirmed by atomic force microscopy, semi-denaturing detergent agarose gel electrophoresis and real-time quaking induced conversion assay (EC
50
= 0.9 and 2.8 µM, respectively). These compounds also disaggregated pre-existing aggregates in vitro and one of them decreased the level of PrP
Sc
in cultured cells with permanent prion infection, suggesting their potential as a treatment platform. In conclusion, hydroxy-2-naphthoylthiosemicarbazides can be an excellent scaffold for the discovery of anti-prion therapeutics.
Design of a Host Interface Logic for GC-Free SSDs Jung, Myoungsoo; Choi, Wonil; Kwon, Miryeong ...
IEEE transactions on computer-aided design of integrated circuits and systems,
08/2020, Letnik:
39, Številka:
8
Journal Article
Recenzirano
Odprti dostop
Garbage collection (GC) and resource contention on I/O buses (channels) are among the critical bottlenecks in solid-state drives (SSDs) that cannot be easily hidden. Most existing I/O scheduling ...algorithms in the host interface logic (HIL) of state-of-the-art SSDs are oblivious to such low-level performance bottlenecks in SSDs. As a result, SSDs may violate quality of service (QoS) requirements by not being able to meet the deadlines of I/O requests. In this paper, we propose a novel host interface I/O scheduler that is both GC aware and QoS aware. The proposed scheduler redistributes the GC overheads across noncritical I/O requests and reduces channel resource contention. Our experiments with workloads from various application domains revealed that the proposed client-level SSD scheduler reduces the standard deviation for latency by 52.5% and the worst-case latency by 86.6%, compared to the state-of-the-art I/O schedulers used for the HIL. In addition, for I/O requests smaller than a superpage, the proposed scheduler avoids channel resource conflicts and reduces latency by 29.2% in comparison to the state-of-the-art I/O schedulers. Furthermore, we present an extension of the proposed I/O scheduler for enterprise SSDs based on the NVMe protocol.
To evaluate correlations between values of articulation tests and language tests for children with articulation disorder in Korea.
Data of outpatients with chief complaint of an articulation problem ...were retrospectively collected. Patients who underwent Urimal Test of Articulation and Phonation (U-TAP) with Assessment of Phonology and Articulation for Children (APAC), Preschool Receptive-Expressive Language Scale (PRES), or Receptive and Expressive Vocabulary Test (REVT) simultaneously were identified. Patients whose word-level percentages of correct consonants in U-TAP (UTAP_wC) were more than 2 standard deviations below the mean as diagnostic criteria for articulation disorder were selected. Those whose receptive language age (P_RLA), expressive language age (P_ELA), or combined language age (P_CLA) in PRES was delayed more than 24 months compared to their chronological age in months as diagnostic criteria for language disorder were excluded.
Thirty-three children aged 3-6 years were enrolled retrospectively. PRES and U-TAP showed significant correlations for most of value relationships. PRES and APAC showed significant correlations for all value relationships except for receptive language age. All values of REVT were significantly correlated with all values from U-TAP, but not with any value from APAC. Articulation tests U-TAP and APAC showed significant correlations between percentages of correct consonants. Language tests PRES and REVT showed significant correlations for all value relationships.
This study suggests that articulation abilities and language abilities might be correlated in children with articulation disorder.
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