Establishing an ultra-high-precision link for time–frequency comparisons between satellites and ground stations is critically important. This endeavor is fundamental to the advancement of pioneering ...space science exploration and the development of a robust space-based time–frequency system featuring ultra-high-precision space atomic clocks. In response to the requirements for assessing the long-term stability of high-precision space atomic clocks, we have designed and implemented a satellite–ground microwave time–frequency comparison system and method based on a three-frequency mode. Ground-based experimental results demonstrate that the equipment layer can achieve a satellite–ground time comparison accuracy better than 0.4 ps (RMS), with the equipment delay stability (ADEV) for all three frequencies being better than 8 × 10−18 at 86,400. By leveraging the ground-based experimental results, we constructed a satellite–ground time–frequency comparison simulation and verification platform. This platform realizes ultra-high-precision satellite–ground time–frequency comparison based on the China Space Station (CSS). After correcting various transmission delay errors, the satellite–ground time comparison achieved an accuracy better than 0.8 ps and an ADEV better than 2 × 10−17 at 86,400. This validation of our novel satellite–ground time–frequency comparison system and method, capable of achieving an 10−17 magnitude stability, is not only a significant contribution to the field of space time–frequency systems but also paves the way for future advancements and applications in space science exploration.
A highly sensitive temperature sensor based on seven-core multicore fiber-polarization maintaining fiber (MCF-PM) structure fiber loop mirror is proposed and experimentally demonstrated. The effects ...of lengths of seven-core MCF and PM fiber on the temperature and strain sensitivities were studied. The results show that both the lengths of the MCF and PM fiber have significant impact on the temperature sensitivity of the sensor while have weak influence on the strain sensitivity. The maximum temperature sensitivity can reach −2.38nm/°C when a 12cm length of MCF and a 10cm length of PM fiber are used. The achieved temperature sensitivity is at nanometer level per Celsius, which is higher than that of the sensors with non-filled or no-coated multicore fiber reported in the literatures. The experiment results also show that the sensor has a high extinction ratio of 36dB due to the adding of the PM fiber. During the temperature stability measurement of 6 hours, the sensor exhibits a stable response to the temperatures with the maximum wavelength deviation of ~0.4nm, equivalent to 0.17°C. <graphic position="float" orientation="portrait" xlink:href="hu10-2946360.eps"/>
A cost-efficient P-D fiber structure-based Sagnac loop sensor is proposed and experimentally demonstrated for measuring temperature and liquid refractive index (RI). The P-D structure is fabricated ...by fusion splicing a section of polarization-maintaining fiber (PMF) to a piece of multimode D-shaped optical fiber (MMDF). Then the P-D structure is built into a Sagnac loop using a 3dB coupler. The temperature and RI characteristics of the sensor are investigated experimentally. The results show that two resonant dips have different spectral responses of temperature and RI, which indicate that the sensor can realize simultaneous temperature and RI measurement. The high sensitivities of -1.804nm/°C and -131.49nm/RIU are achieved. The obtained resolutions of temperature and RI of the proposed sensor can reach 0.01°C and 2.46 × 10
RIU, respectively. The proposed sensor has the potential application in biological and chemical fields.
Presented here is long-range distributed vibration sensing based on internal-modulation optical frequency domain reflectometry (OFDR). In the proposed system with internal modulation, a silicon-based ...photonic-chip laser is used as the laser source, and by controlling the output voltage curve of an arbitrary waveform generator to induce temperature change in the external cavity of the laser, a 10-GHz optical frequency tuning range is achieved. The complexity of the proposed internal-modulation system is lower than that of the traditional external-modulation OFDR system that combines a narrow-linewidth laser with a single-sideband modulator to achieve wavelength tuning. Cross-correlation analysis is used as a sensing mechanism to evaluate the similarity between Rayleigh scatter signals and to achieve vibration event localization. Experimental comparison is made of the vibration sensing performance of the external- and internal-modulation systems, and for a vibration event generated at a distance of 100.95 km, they locate it with a sensing spatial resolution of 43.0 m and 16.8 m, respectively. The results indicates that the proposed distributed vibration sensing based on internal modulation has better sensing performance and lower complexity compared to the traditional external-modulation system. In addition, the proposed system is single-ended and involves no optical amplification, which makes it very suitable for ultra-long-range sensing.
Antibiotics in waste activated sludge (WAS) has drawn increasing attention because of their persistent and bioaccumulation characteristics. Most study illustrated the role of antibiotics in anaerobic ...fermentation from WAS, but lacking the analysis at microbial level as well as the possible interaction between them. This study investigated the effect of three sulfonamide antibiotics (sulfamethoxazole (SMX), sulfaquinoxaline (SQX), and sulfadiazine (SD)) on WAS fermentation and explored its microbiological mechanism. Results indicated that the production of short-chain fatty acids (SCFAs) was significantly improved by 1.9 folds with a peak value at 4626.1 mg COD L−1 in the existence of SD. This was attributed to the promoted release of soluble proteins and polysaccharides with the existence of sulfonamide antibiotics (SAs) as revealed by the excitation-emission matrix (EEM) spectrum. Analysis of microbial community structure showed that the total abundance of the fermenters in groups with SAs was1.2–1.6 times of that in Control. Specifically, the acid-forming genus Tissierella in SMX and SQX increased by 12.1%–15.0% compared with the Control, while the proteolytic genus Proteinivorax dominated in SD with 39.5%. Molecular ecological networks (MENs) analysis further revealed the potential cooperative relationships among different fermenters. This study was anticipated to provide some valuable information for the behavior of antibiotics in WAS fermentation.
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•The existence of SMX, SQX and SD facilitated SCFAs production from WAS anaerobic fermentation.•The presence of SMX, SQX and SD enhanced the solubility and biodegradability of proteins and polysaccharides.•The abundance of fermenters was increased in fermentation systems containing SMX, SQX and SD.•The potential cooperative relationships were established among the key microbial community.
Synergistic control of the risks posed by emerging antimicrobials and antibiotic resistance genes (ARGs) is crucial for ensuring ecological safety. Although electrogenic respiration can enhance the ...biodegradation of several antimicrobials and reduce ARGs accumulation, the association mechanisms of antimicrobial biodegradation (trimethoprim, TMP) with the fate of the antimicrobial resistome remain unclear. Here, the biotransformation pathway of TMP, microbial associations, and functional gene profiles (e.g., degradation, antimicrobial resistance, and electron transfer) were analyzed. The results showed that the microbial electrogenic respiration significantly enhanced the biodegradation of TMP, especially with a cosubstrate sodium acetate supply. Electroactive bacteria enriched in the electrode biofilm positively correlated with potential TMP degraders dominated in the planktonic communities. These cross-niche microbial associations may contribute to the accelerated catabolism of TMP and extracellular electron transfer. Importantly, the evolution and dissemination of overall ARGs and mobile genetic elements (MGEs) were significantly weakened due to the enhanced cometabolic biodegradation of TMP. This study provides a promising strategy for the synergistic control of the water ecological risks of antimicrobials and their resistome, while also highlighting new insights into the association of antimicrobial biodegradation with the evolution of the resistome in an electrically integrated biological process.
•Micron- and nano-droplets of emulsified kerosene were prepared.•Nanodroplets enhanced bubble coalescence and froth drainage better.•Nanodroplets inhibited entrained gangue and favored selective ...flotation of graphite.
In this study, kerosene emulsions with different average droplet sizes (700 nm, 854 nm, and 996 μm) were prepared to investigate their effects on the entrainment of gangue materials and the selectivity index in the flotation of aphanitic graphite. The calculation results based on Neethling and Cillers’s model indicate that a decrease in the average droplet size of emulsified kerosene would result in a decrease in the entrainment of gangue materials, which corresponds to the observations of froth layer height, maximum water recovery rate, and bubble size in the top section of the froth zone. This may be ascribed to the enhanced collision probability between graphite particles and oil droplets, as well as the increased modified flotation rate constant compared to the micro-scale droplets. It is also noted that the selectivity index in graphite flotation increases as the average droplet size of kerosene emulsions decreases. This is largely due to the synthetic effect of the mitigated gangue entrainment and the intensified collision between graphite particles and oil droplets in the presence of nano-scale oil droplets.
In order to achieve a highly sensitive detection of procymidone in vegetables, three paper-based biosensors based on a core biological immune scaffold (CBIS) were developed, which were time-resolved ...fluorescence immunochromatography strips with Europium (III) oxide (Eu-TRFICS). Goat anti-mouse IgG and europium oxide time-resolved fluorescent microspheres formed secondary fluorescent probes. CBIS was formed by secondary fluorescent probes and procymidone monoclonal antibody (PCM-Ab). The first type of Eu-TRFICS (Eu-TRFICS-(1)) fixed secondary fluorescent probes on a conjugate pad, and PCM-Ab was mixed with a sample solution. The second type of Eu-TRFICS (Eu-TRFICS-(2)) fixed CBIS on the conjugate pad. The third type of Eu-TRFICS (Eu-TRFICS-(3)) was directly mixed CBIS with the sample solution. They solved the problems of steric hindrance of antibody labeling, insufficient exposure of antigen recognition region and easy loss of activity in traditional methods. They realized multi-dimensional labeling and directional coupling. They replaced the loss of antibody activity. And the three types of Eu-TRFICS were compared, among which Eu-TRFICS-(1) was the best detection choice. Antibody usage was reduced by 25% and sensitivity was increased by 3 times. Its detection range was 1–800 ng/mL, the limit of detection (LOD) was 0.12 ng/mL with the visible LOD (vLOD) of 5 ng/mL.
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•Time-resolved fluorescent microspheres were applied to CBIS.•The monoclonal antibody usage was reduced by 25% prepared by the CBIS.•The CBIS connect multiple microspheres to one antibody molecule.•The linear range of Eu-TRFICS-(1) used to detect procymidone is 1–800 ng/mL.•Three kinds of Eu-TRFICS used to detect procymidone are constructed and compared.
Persulfate oxidation (PS) is widely employed as a promising alternative for waste activated sludge pretreatment due to the capability of generating free radicals. The product differences and ...microbiological mechanisms by which PS activation triggers WAS digestion through multiple modes need to be further investigated. This study comprehensively investigated the effects of persulfate oxidation activated through multiple modes, i.e., ferrous, zero-valent iron (ZVI), ultraviolet (UV) and heat, on the performance of sludge digestion. Results showed that PS_ZVI significantly accelerated the methane production rate to 12.02 mL/g VSS. By contrast, PS_Heat promoted the sludge acidification and gained the maximum short-chain fatty acids (SCFAs) yield (277.11 ± 7.81 mg COD/g VSS), which was 3.41-fold compared to that in PS_ZVI. Moreover, ferrous and ZVI activated PS achieved the oriented conversion of acetate, the proportions of which took 73% and 78%, respectively. MiSeq sequencing results revealed that PS_Heat and PS_UV evidently enriched anaerobic fermentation bacteria (AFB) (i.e., Macellibacteroides and Clostridium XlVa). However, PS_Ferrous and PS_ZVI facilitated the enrichment of Woesearchaeota and methanogens. Furthermore, molecular ecological network and mantel test revealed the intrinsic interactions among the multiple functional microbes and environmental variables. The homo-acetogens and sulfate-reducing bacterial had potential cooperative and symbiotic relationships with AFB, while the nitrate-reducing bacteria displayed distinguishing ecological niches. Suitable activation modes for PS pretreatments resulted in an upregulation of genes expression responsible for digestion. This study established a scientific foundation for the application of sulfate radical-based oxidation on energy or high value-added chemicals recovery from waste residues.
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•Effect of multiple activated persulfate oxidation on sludge digestion was studied.•Heat and UV activated persulfate effectively promoted the accumulation of SCFAs.•ZVI and Ferrous activated persulfate were beneficial for methane production.•Microbial community structures were altered by multiple persulfate activation modes.•Suitable activation modes for persulfate upregulated the critical genes expression.
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