The emergence of a news recommendation system can effectively improve the news reading experience of users. The most important task of the system is how to learn news and user representations ...accurately. In the process of learning news representations, most of the current research works do not fully utilize the news features, which makes it difficult to learn more comprehensive news representations. Most research work only learns user representations from a single perspective, which may not be sufficient to learn diverse and dynamic user representations. Therefore, we propose a news recommendation system with a multiview graph convolutional network (NRMG). It contains two parts: news representation and user representation. The knowledge-content collaboration network is adapted to learn news representations from news content and entities, while the multiview graph convolutional network (GCN) is utilized to learn user representations from the user's click history. The advantage of the NRMG system is that we not only expand the available features by constructing a subclass knowledge graph (KG), but also effectively improve the ability of the news recommendation system to accurately learn news and user representations. Experimental results on the real dataset MIcrosoft News Dataset (MIND) show that the NRMG achieves a 2.25% improvement in area under the receiver operating characteristic (ROC) curve (AUC) value compared with state-of-the-art methods.
Two 2D wavy hexagonal hexahydroxyl cyclotricatechylene （CTC） based COFs, CTC-COF-2 and CTC-COF-3were synthesized through solvothermal reaction. The bowl-shaped conformation caused CTC skeletons ...packed in acolumnar manner with the same oriented units, thus forming an undulated structure. The gas adsorption propertiesof CTC-COFs were investigated, which show the potential application abilities in hydrogen storage of CTC-COFs.The introduction of pyrene into CTC-COF-3 makes it a potential semiconducting π-conjugated material.
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Recently, zwitterionic materials have been developed as alternatives to PEG for prolonging the circulation time of nanoparticles without triggering immune responses. However, ...zwitterionic coatings also hindered the interactions between nanoparticles and tumor cells, leading to less efficient uptake of nanoparticles by cancer cells. Such effect significantly limited the applications of zwitterionic materials for the purposes of drug delivery and the development to novel therapeutic agents. To overcome these issues, surface-adaptive mixed-shell micelles (MSMs) with poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC)/poly(β-amino ester) (PAE) heterogeneous surfaces were constructed. Owing to the synergistic effect of zwitterionic coatings and micro-phase-separated surfaces, PMPC mixed-shell micelles exhibited the improved blood circulation time compared to single-PEG-shell micelles (PEGSMs) and single-PMPC-shell micelles (PMPCSMs). Moreover, such MSMs can convert their surface to positively charged ones in response to the acidic tumor microenvironment, leading to a significant enhancement in cellular uptake of MSMs by tumor cells. This strategy demonstrated a general approach to enhance the cellular uptake of zwitterionic nanoparticles without compromising their long circulating capability, providing a practical method for improving the tumor-targeting efficiency of particulate drug delivery systems.
Herein we demonstrate a general strategy to integrate non-fouling zwitterionic surface on the nanoparticles without compromising their capability of tumor accumulation, by constructing a surface-adaptive mixed-shell micelles (MSMs) with poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC)/poly(β-amino ester) (PAE) heterogeneous surfaces. At the blood pH (7.4), PAE chains collapsed to the inner of the shell due to the deprotonation, and the forming micro-phase separation structure was synergistic with zwitterionic surface to prolong the circulation time of MSMs in the blood. While at the tumor sites, PAE was protonated, and the positively charged surface of MSMs enhanced cellular uptake. This self-assembly-based strategy is compatible to other zwitterionic materials, endowing a great flexibility for the construction of responsive drug delivery systems particularly to the novel chemotherapeutic agents.
The malignant lung cancer has a high morbidity rate and very poor 5-year survival rate. About 80% - 90% of protein degradation in human cells is occurred through the ubiquitination enzyme pathway. ...Ubiquitin ligase (E3) with high specificity plays a crucial role in the ubiquitination process of the target protein, which usually occurs at a lysine residue in a substrate protein. Different ubiquitination forms have different effects on the target proteins. Multiple short chains of ubiquitination residues modify substrate proteins, which are favorable signals for protein degradation. The dynamic balance adapted to physiological needs between ubiquitination and deubiquitination of intracellular proteins is beneficial to the health of the organism. Ubiquitination of proteins has an impact on many biological pathways, and imbalances in these pathways lead to diseases including lung cancer. Ubiquitination of tumor suppressor protein factors or deubiquitination of tumor carcinogen protein factors often lead to the progression of lung cancer. Ubiquitin proteasome system (UPS) is a treasure house for research and development of new cancer drugs for lung cancer, especially targeting proteasome and E3s. The ubiquitination and degradation of oncogene proteins with precise targeting may provide a bright prospect for drug development in lung cancer; Especially proteolytic targeted chimerism (PROTAC)-induced protein degradation technology will offer a new strategy in the discovery and development of new drugs for lung cancer.
An altered expression of these circRNAs was confirmed using reverse transcription quantitative polymerase chain reaction (RT-qPCR), followed by receiver operating characteristic (ROC) analysis using ...clinical data to evaluate the diagnostic and prognostic potentials of the circRNAs of interest. Liver tissues from 68 patients with BA and 41 patients with CC were immediately stored in RNA later (ThermoFisher, AM7020, Waltham, MA, USA) at −80°C. Sample collection was performed after receiving the consent of patients and relatives, with approval from the ethics committee of Shenzhen Children's Hospital (No. See PDF Moreover, both hsa_circ_0009096 and hsa_circ_0072697 showed higher expression levels in the BA group compared with the CC group; the expression level of hsa_circ_0009096 remained consistent with our circRNA sequencing analysis, whereas hsa_-circ_0072697 was downregulated in BA samples Supplementary Figure 2, http://links.lww.com/CM9/A995.
To discover new potential pesticide candidates, recently, structural modification of natural bioactive products has received much attention. In this work, a series of new piperine-type ester ...derivatives were regio- and stereoselectively synthesized based on a natural alkaloid piperine isolated from
. Their structures were characterized by IR, mp,
H NMR (
C NMR), and high-resolution mass spectrometry (HRMS). Against
Boisduval (Acari: Tetranychidae), compounds
,
,
, and
displayed the most significant acaricidal activity with LC
values of 0.155, 0.117, 0.177, and 0.164 mg/mL, respectively. Particularly, compound
showed >120-fold higher acaricidal activity than piperine (LC
: 14.198 mg/mL). Notably, the acaricidal activity of
was equivalent to that of the commercial acaricide spirodiclofen (LC
: 0.115 mg/mL). Additionally, against
Hausmann (Hemiptera: Aphididae), compounds
and
showed 1.8-fold aphicidal activity of piperine. Furthermore,
the scanning electron microscope (SEM) imaging method, the obvious destruction of the construction of the cuticle layer of
treated
was observed. Compound
could be further studied as a lead acaricidal agent.
Objective: This study aims to compare the efficacy of the potassium-titanyl-phosphate (KTP) laser and cold steel surgery in treating oropharyngeal papilloma. Methods: Between 2017 and 2020, we ...enrolled 242 patients with oropharyngeal papilloma who were treated with either the KTP laser (n = 160) or cold steel surgery (n = 82). Patient charts were reviewed for demographic data (age and gender), pathology, anatomical location of lesions, operative duration, pain rating, residual disease, and recurrence. Results: The oropharyngeal papillomas were successfully removed in all patients, except one with a significant pharyngeal reflex. There was no significant difference in the average time for lesion resection between KTP laser and cold steel group (18.11 ± 13.96 s vs 19.43 ± 16.91 s, P > .05). However, all patients who underwent cold steel surgery experienced bleeding during the operation and required postoperative observation (about 20 min), making the total procedure time longer than that of the KTP laser procedure, which did not cause any intraoperative bleeding or require postoperative observation. After KTP laser treatment, the pain rating was .49 ± .98, whereas after cold steel surgery, it was .74 ± 1.12 (P = .058). Twenty-five samples were sent for human papillomavirus (HPV) testing, and one tested positive for both HPV 6 and 11 strains, while another tested positive for HPV 16. No residual disease or recurrence was observed at the treatment sites after a long period of follow-up (M = 15.35 ± 10.79 mo; range = 6-39 mo). Conclusion: The KTP laser provided a better hemostasis effect and a good surgical field of vision during the operation, allowing the surgeon to complete the procedure in less time. No significant difference in terms of pain rating, incision recovery, and postoperative recurrence between the KTP laser treatment and cold steel surgery.
The damaged endometrium and the formation of fibrosis are key barriers to pregnancy and further lead to infertility. However, how to promote endometrium repair is always a challenge. Here, a ...bioactive injectable and self‐healing hydrogel is developed by physically combination of thiolated polyethylene (PEG), Cu2+ and cell‐free fat extract (CEFFE, CF) for endometrial regeneration and fertility. By inheriting the advantages of various active proteins contained in CEFFE, it could induce the overall repair of endometrial microenvironment for intrauterine adhesion (IUA). In vitro, CF@Cu‐PEG reduces endometrial cell apoptosis by more than 50%, and increases angiogenesis by 92.8%. In the IUA mouse, injection of CF@Cu‐PEG significantly reduces the rate of uterine hydrometra and prevents the formation of endometrial fibrosis. Remarkably, CF@Cu‐PEG contributes to the repair of endometrial microstructure, especially increases the number of endometrial pinopodes, significantly improves endometrial receptivity, and increases the pregnancy rate of IUA mice from 7.14% to 66.67%. In summary, through the physically combination of CEFFE and Cu‐PEG, the construction of loaded bioactive injectable hydrogel not only inhibits the IUA, but also induces the self‐repair of endometrial cells in situ and improves fertility, providing a new strategy for IUA repair in clinical application.
A bioactive injectable and self‐healing hydrogel is developed by non‐covalent binding of Cu2+ to ‐SH and dynamic coordination with physically embedded cell‐free fat extract (CEFFE, CF) for intrauterine adhesion (IUA). It could promote the endometrial regeneration and the recovery of fertility which is expected to be an effective material composite for clinical treatment of IUA.
Cold stimulation reduces the quality of animal products and increases animal mortality, causing huge losses to the livestock industry in cold regions. Long non-coding RNAs (lncRNAs) take part in many ...biological processes through transcriptional regulation, intracellular material transport, and chromosome remodeling. Although cold stress-related lncRNAs have been reported in plants, no research is available on the characteristic and functional analysis of lncRNAs after cold stress in rats. Here, we built a cold stress animal model firstly. Six SPF male Wistar rats were randomly divided to the acute cold stress group (4 °C, 12 h) and the normal group (24 °C, 12 h). lncRNA libraries were constructed by high-throughput sequencing (HTS) using rat livers. 2,120 new lncRNAs and 273 differentially expressed (DE) lncRNAs were identified in low temperature environments. The target genes of DElncRNA were predicted by cis and trans, and then functional and pathway analysis were performed to them. GO and KEGG analysis revealed that lncRNA targets were mainly participated in the regulation of nucleic acid binding, cold stimulation reaction, metabolic process, immune system processes, PI3K-Akt signaling pathway and pathways in cancer. Next, a interaction network between lncRNA and its targets was constructed. To further reveal the mechanism of cold stress, DElncRNA and DEmRNA were extracted to reconstruct a co-expression sub-network. We found the key lncRNA MSTRG.80946.2 in sub-network. Functional analysis of key lncRNA targets showed that targets were significantly enriched in fatty acid metabolism, the PI3K-Akt signaling pathway and pathways in cancer under cold stress. qRT-PCR confirmed the sequencing results. Finally, hub lncRNA MSTRG.80946.2 was characterized, and verified its relationship with related mRNAs by antisense oligonucleotide (ASO) interference and qRT-PCR. Results confirmed the accuracy of our analysis. To sum up, our work was the first to perform detailed characterization and functional analysis of cold stress-related lncRNAs in rats liver. lncRNAs played crucial roles in energy metabolism, growth and development, immunity and reproductive performance in cold stressed rats. The MSTRG.80946.2 was verified by network and experiments to be a key functional lncRNA under cold stress, regulating ACP1, TSPY1 and Tsn.
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